Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,951,207 C→G 89.7% A129A (GCG→GCC ssb1 ← single‑stranded DNA‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,951,2070C→G89.7% 31.7 ‑0.5 0.0136A129A (GCG→GCCssb1single‑stranded DNA‑binding protein
Reads supporting (aligned to +/- strand):  new base (68/54):  ref base (9/5):  total (77/59)
Fisher's exact test strand distribution p-value = 5.84e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

GCCCGACGAGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGCGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CGCG.CGTAATTGCCACCGCCGCGCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCGT  >  NC_014034/1951114‑1951299
                                                                                              |                                                                                             
gccCGACGAGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAAt                                                                                       >  1:522644/1‑101 (MQ=255)
cccggcggggggggCCAAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAAt                                                                                       <  2:3665161/92‑1 (MQ=255)
ccccGAAGGGGGGGCGGAGGACCCCCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAAt                                                                                       <  1:1112113/100‑1 (MQ=255)
 cccGACGGGGGGCCCGAGGACCCCCCCCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAAtt                                                                                      <  1:3600568/101‑1 (MQ=255)
  ccGACGAGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTg                                                                                     >  2:2560194/1‑101 (MQ=255)
    gcggggggggCCGGGGACCCCCCCCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGcc                                                                                   <  2:4751800/96‑1 (MQ=255)
    gcgggggggcCCGAGGAGCCCCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGcc                                                                                   <  2:1736161/96‑1 (MQ=255)
       ggggtggCGGAGGAGCCGCCCCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCCCCATAGCCGCCGCCCTGCGAACCG.C.CGCCGTAATTGCCAcc                                                                                <  2:3099518/96‑1 (MQ=255)
       gggggggCCGAGGAGCCGCCGCCACCGCCGCCATGGCTCGGGCCGTCATCGTAGCCGGCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCAcc                                                                                <  1:4315416/99‑1 (MQ=255)
       gaGGGGGCGGAGGCGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.C.CGCCGTAATTGCCAcc                                                                                <  2:2993865/101‑1 (MQ=255)
         gggggCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CTGC.CGTAATTGCCAccgc                                                                              >  2:4540731/1‑101 (MQ=255)
          gggcCCGGGGACCCCCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.C.CGCCGTAATTGCCAccgcc                                                                             <  1:2247639/101‑1 (MQ=255)
            ggCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACcgccgc                                                                           >  1:4078702/1‑101 (MQ=255)
             ggggagctgccccccccCCCGCGCCCATAGCCCGGGCCGCCCCCGTCGCCGCCGCCATAGCCGCCGCCCTGCGACCCG.CCGC.CGTAATTGCCACcgccgcc                                                                          <  1:1313220/93‑2 (MQ=255)
             gCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACcgccgcc                                                                          >  1:1369301/1‑100 (MQ=255)
              cggaggccccgccccccccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACcgccgccc                                                                         <  1:4125664/93‑3 (MQ=255)
              ccGAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACcgccgccc                                                                         >  2:2965651/1‑99 (MQ=255)
                gaggagCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGa                                                                       >  1:3909836/1‑101 (MQ=255)
                gaggagCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CAGC.CGTAATTGCCACCGCCGCCCGa                                                                       >  2:2781164/1‑101 (MQ=255)
                 aggagCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAg                                                                      >  1:2544683/1‑101 (MQ=255)
                  ggacCCGCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAgc                                                                     <  1:12688/101‑1 (MQ=255)
                   gagCCGCCCCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAgcc                                                                    <  1:79574/101‑1 (MQ=255)
                       cgccgccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAgccgccg                                                                >  1:3561904/1‑101 (MQ=255)
                       ccccgcccccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAgccgccg                                                                <  2:4205187/99‑1 (MQ=255)
                         ccgccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAgccgccgcc                                                              >  2:4947842/1‑101 (MQ=255)
                          cgcccccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCa                                                             <  2:594984/101‑1 (MQ=255)
                          cgccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCa                                                             >  2:216888/1‑101 (MQ=255)
                          cgccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCa                                                             >  2:2283140/1‑101 (MQ=255)
                           ccccccgccgccaTAGCCGGGGCCGTCATCTTAGCCGCCGCCATAGCCGCCGCCCGGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCAt                                                            <  2:2025409/97‑1 (MQ=255)
                            ccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATc                                                           >  1:2819882/1‑101 (MQ=255)
                            ccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATc                                                           >  1:1577058/1‑101 (MQ=255)
                              ccccccgccaTAGCCCGGGCCGCCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATccc                                                         <  2:508336/97‑1 (MQ=255)
                              accgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATccc                                                         >  2:4106589/1‑101 (MQ=255)
                               ccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCg                                                        <  1:5017689/101‑1 (MQ=255)
                               cccccgccaTAGCCCGGGCCGTCATCTTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCg                                                        <  1:2952003/98‑1 (MQ=255)
                                  ccgccaTGGCCCGGCCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgcc                                                     <  2:4592788/101‑1 (MQ=255)
                                  ccgccaTCGCCCGGGCCGTCATCGTAGCCGCCCCCATAGCCCCCCCCCTGCCAACCC.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgcc                                                     <  1:117253/101‑1 (MQ=255)
                                  ccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgcc                                                     >  1:2118691/1‑101 (MQ=255)
                                   cccaagaGCCCGGCCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccg                                                    <  1:977017/95‑1 (MQ=255)
                                     ccaTGGCCGGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccgcc                                                  <  1:236186/101‑1 (MQ=255)
                                     ccaTAGCCCGGGCCGTCATCGTAGCCGCCGCTATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccgcc                                                  >  1:4478662/1‑101 (MQ=255)
                                     ccaTAGCCCGGCCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccgcc                                                  <  1:2560375/101‑1 (MQ=255)
                                       agccccggggcCGCCATCTTAGCCCCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCaa                                                <  2:1297540/92‑1 (MQ=255)
                                        tcgCCCGGGCCGTCATCGTACCCCCCGCCATAGCCGCCGCCCTGCGACCCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAag                                               <  1:4683319/99‑1 (MQ=255)
                                         gccccGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagc                                              <  1:2010051/99‑1 (MQ=255)
                                         aGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagc                                              >  1:2456081/1‑101 (MQ=255)
                                           cgggggcCGTAATCGAACCCCCCGCAATAGCCCCCGCCCTGAAAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagcag                                            <  1:4793879/96‑1 (MQ=255)
                                           cccGGGCCGTCATCTTCGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagcag                                            <  2:1278606/101‑1 (MQ=255)
                                            ccGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagcagc                                           >  1:714159/1‑101 (MQ=255)
                                             cgggcCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCg                                          >  2:181883/4‑101 (MQ=255)
                                              gggcCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGt                                         >  1:3754943/3‑101 (MQ=255)
                                              ggacagagagcGTAGCGGCCCCAAAAGCCGCCGCCCTGCGACCCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGt                                         <  2:1716243/91‑1 (MQ=255)
                                               ggcggTCATCGTAGCCCCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTc                                        <  1:53285/97‑1 (MQ=255)
                                               ggcCGTCTTCGTAGCCCCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTc                                        <  1:580680/100‑1 (MQ=255)
                                               gcccGTCATGGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTc                                        <  1:4141882/99‑1 (MQ=255)
                                                 ccGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAg                                      >  1:4696685/1‑101 (MQ=255)
                                                  cGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAgg                                     >  2:4356556/1‑101 (MQ=255)
                                                  cGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAgg                                     >  2:3459928/1‑101 (MQ=255)
                                                   gTCACCGTAGCCGCCGCCATACCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGt                                    <  2:1397507/101‑1 (MQ=255)
                                                    taaccGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGtt                                   <  1:1668799/97‑1 (MQ=255)
                                                    taaacGTGGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGtt                                   <  1:1686649/97‑1 (MQ=255)
                                                    tCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGACCCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGtt                                   <  2:3741628/101‑1 (MQ=255)
                                                    tCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGtt                                   >  2:1936247/1‑101 (MQ=255)
                                                     cATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTg                                  >  2:3846006/1‑101 (MQ=255)
                                                      aTCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGc                                 >  2:4331900/1‑101 (MQ=255)
                                                       tCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGcc                                >  2:1139716/1‑101 (MQ=255)
                                                        cGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGccc                               <  1:1059329/101‑1 (MQ=255)
                                                        cGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGccc                               >  1:3261406/1‑101 (MQ=255)
                                                        cGTACCCCCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGccc                               <  2:1730435/101‑1 (MQ=255)
                                                         gtcccggccgccAAAGCCCCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCg                              <  1:3758967/95‑1 (MQ=255)
                                                         gTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCACGCAGCGTCAGGTTGCCCg                              >  1:1163813/1‑101 (MQ=255)
                                                         gTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCg                              >  1:1959631/1‑101 (MQ=255)
                                                         gTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCg                              >  2:233357/1‑101 (MQ=255)
                                                         gTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCg                              >  1:4554358/1‑101 (MQ=255)
                                                          tAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGt                             >  2:2958629/1‑101 (MQ=255)
                                                           ccccgccccaATACCCGCCCCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGta                            <  2:751867/99‑1 (MQ=255)
                                                           aGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGta                            >  2:732261/1‑101 (MQ=255)
                                                           aGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGta                            >  2:2064770/1‑101 (MQ=255)
                                                           aGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGta                            >  2:4441941/1‑101 (MQ=255)
                                                              cccccccATAGCCGCCGCCCTGCGACCCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAg                         <  2:2819669/96‑1 (MQ=255)
                                                               gccgccATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAgg                        >  1:2138359/1‑101 (MQ=255)
                                                               cccgccATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAgg                        <  1:4630360/100‑1 (MQ=255)
                                                                  gccATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCACCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgc                     >  2:2569375/1‑101 (MQ=255)
                                                                   ccATAGCCGCCGCCCTGCGAACCG.C.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcg                    >  2:1751580/1‑101 (MQ=255)
                                                                   ccATAGCCGCCGCCCTGCGAACCG.C.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcg                    >  1:3245491/1‑101 (MQ=255)
                                                                    catgcccgccgccCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcgc                   <  2:4014088/96‑1 (MQ=255)
                                                                    cATAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcgc                   >  1:4920200/1‑101 (MQ=255)
                                                                     aTAGCCGCCGCCCTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgca                  >  2:201863/1‑101 (MQ=255)
                                                                     aTAGCCGCCGCCCTGCGAACCG.C.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgca                  >  2:102774/1‑101 (MQ=255)
                                                                      tAGCCGCCGCCCTGCGAACCGACGC..CGTAATTGCCACCGCCGCCCGAGCAGCCGCCATCCCGGCCGCCAAGCAGCGGCAGGTTGCCGGTATAGGGGCgcag                 >  2:2101619/1‑101 (MQ=255)
                                                                       agcccnnccccTGCGACCCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcagc                <  1:1820125/93‑1 (MQ=255)
                                                                       aGCCGCCGCCCTGCGAACCG.C.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcagc                >  2:3111853/1‑101 (MQ=255)
                                                                              gccCTGCGAACCG.C.CGCCGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCt         >  1:3595600/1‑101 (MQ=255)
                                                                              ccccTGCGACCCC.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCt         <  1:229354/100‑1 (MQ=255)
                                                                               cccTGCGACCCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTc        <  1:774735/101‑1 (MQ=255)
                                                                               cccTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCCTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTc        <  1:4173347/101‑1 (MQ=255)
                                                                                 cTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCgg      >  1:2955938/1‑101 (MQ=255)
                                                                                 cTGCGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCgg      >  1:3937419/1‑101 (MQ=255)
                                                                                    cGAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCg   >  1:1607412/1‑101 (MQ=255)
                                                                                     gAACCG.CCGC.CGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCgt  >  1:2207033/1‑101 (MQ=255)
                                                                                              |                                                                                             
GCCCGACGAGGGGGCCGAGGAGCCGCCGCCACCGCCGCCATAGCCCGCGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCG.CGCG.CGTAATTGCCACCGCCGCGCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCGT  >  NC_014034/1951114‑1951299

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG