BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,976,894	C→G	91.5%	intergenic (‑73/+7)	fba ← / ← gap1	fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,976,894	0	C→G	91.5%	40.1 ‑2.1 ‑0.4	212	intergenic (‑73/+7)	fba/gap1	fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand): new base (127/67): ref base (8/10): total (135/77)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.36e-01

Only 100 of 223 total aligned reads displayed.


AGTTGCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CGGCG.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976796‑1976990


       gAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCa                                                                                         >  1:1037549/1‑101 (MQ=255)


        aaGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAg                                                                                        >  2:5030993/1‑101 (MQ=255)


        aaGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAg                                                                                        >  2:4881578/1‑101 (MQ=255)


        aaGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAg                                                                                        >  2:3495253/1‑101 (MQ=255)


          gggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGAt                                                                                      >  2:466055/1‑101 (MQ=255)


           ggTGATCAGTGCCTTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATc                                                                                     <  1:1463792/101‑1 (MQ=25)


            gTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCa                                                                                    >  2:1347691/1‑101 (MQ=255)


            gTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.AA.G.CG.CCAC.CGGC..TTTTCTCAGATCa                                                                                    >  1:2012466/1‑101 (MQ=37)


             tGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAg                                                                                   >  1:4090534/1‑101 (MQ=255)


              gATCAGTGCCAGTTTCTCAGTTCCTTGCGTTCGCGCCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CGCCC.C.CGGCG..TTTCTCAGATCAGc                                                                                  >  2:3355080/1‑101 (MQ=21)


                 ccgTGCCTTTTTCTCGGTTCCTTGGTTTGGGGGCTTTTTTTTTGGCCAAAAACCCCCGGGGGCCCGGGGG.CA.G.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTg                                                                               <  1:2373218/99‑1 (MQ=255)


                 cAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTg                                                                               >  1:4291529/1‑101 (MQ=255)


                 cAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTg                                                                               >  1:483490/1‑101 (MQ=255)


                 cAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTg                                                                               >  2:2558844/1‑101 (MQ=255)


                   gTGCCATTTTCTCAGTTCCTGGAGTGCGCGGCCTTTTTCTTGGCCGAAATACGCGGGGGGTCGGGGGG.CA.G.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGcc                                                                             <  2:1335446/101‑1 (MQ=255)


                    tGCCATTTTCTCAGTTCCTGGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGccc                                                                            <  2:855785/101‑1 (MQ=25)


                      cctttttCTCGTTTCCTTGAGTTCGCGGCTTTTTTCTTGGCCAAAATCCGCGGGGGGTCGGGGGG.CA.G.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCAt                                                                          <  1:579775/98‑1 (MQ=255)


                      ccATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCAt                                                                          >  2:4946793/1‑101 (MQ=255)


                       cATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATc                                                                         <  1:4968504/101‑1 (MQ=37)


                         ttttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGc                                                                       >  2:280059/1‑101 (MQ=255)


                           ttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCa                                                                     >  1:2693925/1‑101 (MQ=255)


                            tctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.CGCGGC..TTTTCTCAGATCAGATTGCCGATCGCCAc                                                                    >  1:4362937/1‑101 (MQ=25)


                            tctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    >  2:1552808/1‑101 (MQ=255)


                             ctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATAAGCTTGCCCATCGCCaag                                                                   >  2:776944/1‑99 (MQ=25)


                                 gTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCAcggcgg                                                               >  2:3457472/1‑101 (MQ=255)


                                   tCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  1:3473021/1‑101 (MQ=255)


                                   tCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  2:3113047/1‑101 (MQ=255)


                                   tCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  1:1901015/1‑101 (MQ=255)


                                    ccTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTAt                                                            <  2:3207235/101‑1 (MQ=255)


                                    ccTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTAt                                                            >  1:4894207/1‑101 (MQ=255)


                                      ttGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCg                                                          >  1:4143675/1‑101 (MQ=255)


                                       tGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         >  1:4189820/1‑101 (MQ=255)


                                        gAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCCGATCAGCTTGCCCATAGGCACGGGGGTCTCGGc                                                        >  2:3968969/1‑101 (MQ=21)


                                        gAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGc                                                        >  2:4445526/1‑101 (MQ=255)


                                        gAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGc                                                        >  1:4567627/1‑101 (MQ=255)


                                        gAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGc                                                        >  2:4703678/1‑101 (MQ=255)


                                          gTTCGGGCCCTTTTTGTTGCCCGACAAGCGCCGGCGTTCCGGGCT.AC.G.CC.CC.C.CCGGC.TTTGCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCa                                                      <  2:3671029/101‑1 (MQ=9)


                                          gTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCa                                                      >  2:2382854/1‑101 (MQ=255)


                                          gTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCa                                                      >  2:2451316/1‑101 (MQ=255)


                                          gTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCa                                                      >  1:2432788/1‑101 (MQ=255)


                                            ttggggCCTTTTTTGTGGGCAAAAACCCCCGGGGGGCGGGGGGCCC.G.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCAt                                                     <  1:4561972/97‑1 (MQ=12)


                                                ggCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGt                                                >  1:2630936/1‑101 (MQ=255)


                                                  ccTTTTTTTTGGGCAAAATGCCGCGGGGGGGGGGGGG.CC.GCCC.CC.C.CGGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGtt                                               <  1:379648/101‑1 (MQ=12)


                                                   ttttttTTTGGCCAAAATGCGCGGGGGGCCGGGGGG.CG.G.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             <  1:2509557/100‑1 (MQ=18)


                                                   tttttcCTTGGCCGAAATCCGGGGGGGGTCGGGGGG.CA.C.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             <  1:3296459/100‑1 (MQ=18)


                                                   cTTTTTCTGGGCCGAATTCCGCGGGGGGCCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             <  1:2311652/101‑1 (MQ=21)


                                                       ttCTTGGCCGAAATCCGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         <  2:2505307/101‑1 (MQ=25)


                                                       ttCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         <  2:433495/101‑1 (MQ=255)


                                                       ttCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGGTCGAGaa                                         >  2:3687659/1‑101 (MQ=37)


                                                        tctggggcaaaaTCCGCCGGGGGTCGGGGGG.CA.G.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAg                                        <  1:2878812/92‑1 (MQ=18)


                                                        tctggcccGAAATACGCGGGGGGTCGGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAg                                        <  2:2050734/95‑1 (MQ=21)


                                                        tCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGGATCGGCCATGCGGTTCGAGAAg                                        >  2:2213867/1‑101 (MQ=37)


                                                         tttGGCCGAAATCCGCGGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGc                                       <  2:4090231/100‑1 (MQ=25)


                                                         cTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGc                                       >  1:275434/1‑101 (MQ=255)


                                                         cTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGc                                       >  1:3625380/1‑101 (MQ=255)


                                                          ttGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcc                                      >  1:1219276/1‑101 (MQ=255)


                                                           tGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGccc                                     >  1:1625984/1‑101 (MQ=255)


                                                             gCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCca                                   >  2:1418601/1‑101 (MQ=255)


                                                              ccGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGCATCGGCCATGCGGTTCGAGAAGCCCcat                                  >  1:2980633/1‑101 (MQ=25)


                                                              ccGAAAGACGGCGGGGG.CCGGGGG.CACG.CG.CC.C.CTGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcat                                  <  1:4704001/101‑1 (MQ=18)


                                                               cGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcatt                                 >  2:2283631/1‑101 (MQ=255)


                                                                gAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                >  2:1096814/1‑101 (MQ=255)


                                                                 aaaTACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               >  1:1329154/1‑101 (MQ=255)


                                                                  aaTACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              >  1:1117726/1‑101 (MQ=255)


                                                                  aaTACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              >  1:1736009/1‑101 (MQ=255)


                                                                   aTACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             >  1:411027/1‑101 (MQ=255)


                                                                   aTACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             >  2:4226907/1‑101 (MQ=255)


                                                                   aTACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             >  2:2115652/1‑101 (MQ=255)


                                                                    tcggCCGGGGCTCCGGGGG.CA.G.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTg                            <  2:1238611/98‑1 (MQ=18)


                                                                     ccGCGGGGGGTCCGGGGC.CA.C.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGt                           <  2:4656673/100‑1 (MQ=18)


                                                                     aCGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGt                           >  2:4847166/1‑101 (MQ=255)


                                                                       gCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         >  2:4725370/1‑101 (MQ=255)


                                                                       cgcGGGGGTCCGGGGGCCG.G.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTc                          <  1:3092708/99‑1 (MQ=17)


                                                                        tcGGGGGTCGCGGGG.CA.G.CC.CC.C.CGGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        <  1:1482788/100‑1 (MQ=18)


                                                                        ccGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        <  2:4773535/101‑1 (MQ=255)


                                                                        ccGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        >  2:3006445/1‑101 (MQ=255)


                                                                         cGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATa                       >  2:665285/1‑101 (MQ=255)


                                                                         cGGGGGTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATa                       >  1:3901052/1‑101 (MQ=255)


                                                                          gggggTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAc                      >  1:1954997/1‑101 (MQ=255)


                                                                           ggggTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAcc                     <  2:581528/101‑1 (MQ=255)


                                                                           ggggTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAcc                     <  2:2905499/101‑1 (MQ=255)


                                                                            ggggggggggg.CC.C.CC.CC.C.CGGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAcc                     <  1:84529/95‑1 (MQ=17)


                                                                            gggTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCa                    <  2:4101365/101‑1 (MQ=255)


                                                                             gggcgggggg.CC.G.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCAc                   <  1:2559025/96‑1 (MQ=18)


                                                                             ggTCCGGGGG.CA.C.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCAc                   <  2:796246/101‑1 (MQ=18)


                                                                              gTCCGGGGG.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  >  2:3720375/1‑101 (MQ=255)


                                                                                ccGGGGG.CA.C.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGAc                <  2:5052146/101‑1 (MQ=18)


                                                                                  ggggg.CA.G.CGCCC.C.CGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAg              >  2:1800852/1‑101 (MQ=255)


                                                                                   gggg.CA.G.CC.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAgg             <  1:1531469/101‑1 (MQ=21)


                                                                                     gg.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGAt           >  1:3339474/1‑101 (MQ=255)


                                                                                      g.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATg          >  2:4009217/1‑101 (MQ=255)


                                                                                      g.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATg          <  1:658862/101‑1 (MQ=255)


                                                                                      g.CA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATg          >  1:4738214/1‑101 (MQ=255)


                                                                                        cA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         >  1:1277145/1‑101 (MQ=255)


                                                                                        cA.G.CG.CC.C.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         >  1:2182310/1‑101 (MQ=255)


                                                                                          cagccccc.c.cGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         <  2:1985571/95‑1 (MQ=255)


                                                                                           cgccccc.c.cGGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         <  2:2630751/96‑1 (MQ=255)


                                                                                           ccccccc.c.cGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg        <  2:172154/96‑1 (MQ=255)


                                                                                               ccc.c.cGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    <  2:3924778/100‑1 (MQ=255)


                                                                                               ccc.c.cGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCAATCATTGTCATACCACGACAGGATGCGGAcc    >  2:4414902/2‑101 (MQ=255)


AGTTGCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGG.CA.G.CG.CC.C.CGGCG.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976796‑1976990

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG