Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,977,552 G→A 100% V117V (GTC→GTT gap1 ← glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,977,5520G→A100.0% 994.3 254V117V (GTC→GTTgap1glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (135/119):  ref base (0/0):  total (135/119)

Only 100 of 267 total aligned reads displayed.

GAGGCGTTCGACACGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGGACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGCCCCAGGGCA  >  NC_014034/1977454‑1977649
                                                                                                  |                                                                                                 
gaggCGTTCGACACGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAAc                                                                                                 <  2:1691536/101‑1 (MQ=255)
gaggCGTTCGACACGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAAc                                                                                                 <  1:3308160/101‑1 (MQ=255)
     gTTCGACACGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCt                                                                                            >  1:1021912/1‑101 (MQ=255)
      ttCGACACGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCtt                                                                                           >  1:3658418/1‑101 (MQ=255)
      ttCGACACGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCtt                                                                                           <  2:2919481/101‑1 (MQ=255)
       tCGACACGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTc                                                                                          <  1:3546788/101‑1 (MQ=255)
       tCGACACGACCAGCTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTc                                                                                          <  1:4343780/101‑1 (MQ=255)
        cGACACGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCg                                                                                         <  2:2068520/101‑1 (MQ=255)
        cGACACGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGAGCTTCg                                                                                         >  2:1937116/1‑101 (MQ=255)
          acacGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAg                                                                                       >  1:2366848/1‑101 (MQ=255)
           cacGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGc                                                                                      >  2:609047/1‑101 (MQ=255)
             cGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCa                                                                                    <  1:746420/101‑1 (MQ=255)
              gACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCAt                                                                                   >  2:3242508/1‑101 (MQ=255)
                ccAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCAttt                                                                                 >  1:573584/1‑101 (MQ=255)
                  aGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCAtttag                                                                               >  1:3251947/1‑99 (MQ=255)
                   ggTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGc                                                                              <  1:2075182/101‑1 (MQ=255)
                   ggTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGc                                                                              >  1:194611/1‑101 (MQ=255)
                   ggTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGc                                                                              <  2:2172131/101‑1 (MQ=255)
                    gTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCa                                                                             >  2:1131275/1‑101 (MQ=255)
                     tCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAg                                                                            <  2:4558497/101‑1 (MQ=255)
                     tCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAg                                                                            >  1:991881/1‑101 (MQ=255)
                     tCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAg                                                                            >  1:4036116/1‑101 (MQ=255)
                      cTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGa                                                                           >  1:1792801/1‑101 (MQ=255)
                        tCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATg                                                                         >  1:2947661/1‑101 (MQ=255)
                          ggCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATggg                                                                       <  1:2426399/101‑1 (MQ=255)
                          ggCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATggg                                                                       >  2:16077/1‑101 (MQ=255)
                           gCACTCAGCGTGGCGGGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGc                                                                      >  1:4259260/1‑101 (MQ=255)
                            cACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGcc                                                                     <  2:2725645/101‑1 (MQ=255)
                              cTCGGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCcgc                                                                   <  2:4401220/101‑1 (MQ=255)
                               tCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCcgcg                                                                  <  2:4677969/101‑1 (MQ=255)
                                cAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCgg                                                                 >  2:3030420/1‑101 (MQ=255)
                                 aGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGc                                                                <  2:2908648/101‑1 (MQ=255)
                                 aGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGc                                                                <  2:68057/101‑1 (MQ=255)
                                 aGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGc                                                                >  1:4163124/1‑101 (MQ=255)
                                    gtggcgtggTTCACGCCAAAAACGATCTTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCtt                                                             <  2:1279941/101‑1 (MQ=255)
                                     tggcgtggTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCGGATGGGCCGCGGCCTtc                                                            >  1:1551526/1‑101 (MQ=255)
                                     tggcgtggTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTtc                                                            <  2:4074223/101‑1 (MQ=255)
                                         gtggTTCACGCCAAAAACGATCGTCTTGTCGTCATTGTCGCAGGGCGCCGAGACCAGAACGCGGTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCt                                                        >  1:4312962/1‑101 (MQ=255)
                                         gtggTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCt                                                        >  2:1813438/1‑101 (MQ=255)
                                           ggTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTtg                                                      >  1:4924872/1‑101 (MQ=255)
                                           ggTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTtg                                                      <  1:4782517/101‑1 (MQ=255)
                                           ggTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTtg                                                      >  1:3118052/1‑101 (MQ=255)
                                           ggTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTtg                                                      >  2:4950477/1‑101 (MQ=255)
                                            gTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTtgg                                                     >  2:2480294/1‑101 (MQ=255)
                                             ttCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTtggt                                                    >  2:2662206/1‑101 (MQ=255)
                                              tCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTtggtg                                                   >  2:3750031/1‑101 (MQ=255)
                                                aCGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTggtggt                                                 <  2:2050539/101‑1 (MQ=255)
                                                  gCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGa                                               <  2:2255711/101‑1 (MQ=255)
                                                    cAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAg                                             >  1:4722903/1‑101 (MQ=255)
                                                    cAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAg                                             >  1:1261338/1‑101 (MQ=255)
                                                      aaaaCGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGAt                                           <  1:2695422/101‑1 (MQ=255)
                                                      aaaaCGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGAt                                           <  2:3658146/101‑1 (MQ=255)
                                                       aaaCGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATg                                          <  2:3899086/101‑1 (MQ=255)
                                                       aaaCGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATg                                          <  2:1860309/101‑1 (MQ=255)
                                                         aCGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGcc                                        >  1:4076480/1‑101 (MQ=255)
                                                         aCGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGcc                                        >  1:3371962/1‑101 (MQ=255)
                                                           gATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCgg                                      >  1:3847845/1‑101 (MQ=255)
                                                              cGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGc                                   >  1:1555107/1‑101 (MQ=255)
                                                               gTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGACGCCGGTGCa                                  >  2:91901/1‑101 (MQ=255)
                                                                 cTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCAtt                                >  1:1715769/1‑101 (MQ=255)
                                                                 cTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCAtt                                >  2:5019521/1‑101 (MQ=255)
                                                                 cTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCAtt                                >  1:530573/1‑101 (MQ=255)
                                                                  ttGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTc                               >  2:689442/1‑101 (MQ=255)
                                                                  ttGGCGGCAGTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTc                               <  2:1527607/101‑1 (MQ=255)
                                                                   tGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTcc                              <  2:2367440/101‑1 (MQ=255)
                                                                   tGTCGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTcc                              <  2:394590/101‑1 (MQ=255)
                                                                   tGTCGGCATTGTCGCAGGCCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTcc                              <  2:1219159/101‑1 (MQ=255)
                                                                      cGGCATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAgc                           >  1:3394566/1‑101 (MQ=255)
                                                                         cATTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGcgcg                        >  1:53932/1‑101 (MQ=255)
                                                                          aTTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcga                       >  1:2593167/1‑101 (MQ=255)
                                                                          aTTGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcga                       >  1:2783449/1‑101 (MQ=255)
                                                                           ttGTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgac                      <  1:2245475/101‑1 (MQ=255)
                                                                             gTCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacat                    >  2:2369909/1‑101 (MQ=255)
                                                                              tCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacatc                   >  1:4674141/1‑101 (MQ=255)
                                                                              tCGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacatc                   <  2:479002/101‑1 (MQ=255)
                                                                              gcGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacatc                   <  1:2193788/100‑1 (MQ=255)
                                                                               cGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacatcg                  >  1:2657435/1‑101 (MQ=255)
                                                                               cGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacatcg                  >  2:3349976/1‑101 (MQ=255)
                                                                               cGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacatcg                  >  1:3795221/1‑101 (MQ=255)
                                                                               cGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacatcg                  >  1:3888516/1‑101 (MQ=255)
                                                                               cGCAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacatcg                  <  2:1762473/101‑1 (MQ=255)
                                                                                 cAGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacatcgac                >  1:3039187/1‑101 (MQ=255)
                                                                                  aGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacatcgaca               <  2:2788924/101‑1 (MQ=255)
                                                                                  aGGGCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacatcgaca               >  2:3577577/1‑101 (MQ=255)
                                                                                   gggCGCCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGcgacatcgacat              <  1:3214255/101‑1 (MQ=255)
                                                                                      cgtcGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGc           <  2:4258943/98‑1 (MQ=255)
                                                                                      cgcCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACAtttc           >  1:4540700/1‑99 (MQ=255)
                                                                                       gcCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGcc          >  1:1131023/1‑101 (MQ=255)
                                                                                       gcCGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGcc          >  1:1043599/1‑101 (MQ=255)
                                                                                         cGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGcccc        <  1:2382102/101‑1 (MQ=255)
                                                                                         cGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGcccc        >  1:1306189/1‑101 (MQ=255)
                                                                                         cGAGACCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGcccc        <  1:66691/101‑1 (MQ=255)
                                                                                          gagaCCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGCCCCa       <  2:4162807/101‑1 (MQ=255)
                                                                                          gagaCCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGCCCCa       >  1:1445895/1‑101 (MQ=255)
                                                                                           agaCCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGCCCCAg      <  2:1554977/101‑1 (MQ=255)
                                                                                             aCCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGCCCCAggg    <  2:3117814/101‑1 (MQ=255)
                                                                                             aCCAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGCCCCAggg    >  1:496876/1‑101 (MQ=255)
                                                                                              ccAGAACGCGTTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGCCCCAGGGc   <  2:1924519/101‑1 (MQ=255)
                                                                                              ccAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGCCCCAGGGc   <  1:3586775/101‑1 (MQ=255)
                                                                                              ccAGAACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGCCCCAGGGc   >  2:820213/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GAGGCGTTCGACACGACCAGGTCTTCGGCACTCAGCGTGGCGTGGTTCACGCCAAAAACGATCGTCTTGTCGGCATTGTCGCAGGGCGCCGAGACCAGGACGCGCTTCGAGCCATTTTGCAGATGGGCCGCGGCCTTCTCCTTGGTGGTGAAGATGCCGGTGCATTCCAGCGCGACATCGACATTGCCCCAGGGCA  >  NC_014034/1977454‑1977649

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG