BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,990,962	T→A	100%	Y555F (TAC→TTC)	glgB ←	1,4‑alpha‑glucan branching protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,990,962	0	T→A	100.0%	1125.7	284	Y555F (TAC→TTC)	glgB	1,4‑alpha‑glucan branching protein
Reads supporting (aligned to +/- strand): new base (137/146): ref base (0/0): total (137/147)

Only 100 of 287 total aligned reads displayed.


GAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGTACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACT  >  NC_014034/1990864‑1991059


gAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAAc                                                                                                 >  2:689992/1‑101 (MQ=255)


 aaGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACg                                                                                                <  1:3874973/101‑1 (MQ=255)


    gTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGGTTCTTGCCCGGATGCGGCCATTGGAACGCAt                                                                                             <  2:2774106/101‑1 (MQ=255)


    gTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCAt                                                                                             <  2:1028065/101‑1 (MQ=255)


    gTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCAt                                                                                             <  2:3965524/101‑1 (MQ=255)


    gTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCAt                                                                                             <  1:1567775/101‑1 (MQ=255)


    gTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCAt                                                                                             >  1:3726921/1‑101 (MQ=255)


    gTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCAt                                                                                             <  1:2792508/101‑1 (MQ=255)


    gTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCAt                                                                                             <  1:2718084/101‑1 (MQ=255)


     tGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATa                                                                                            >  1:693753/1‑101 (MQ=255)


     tGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATa                                                                                            <  2:4905603/101‑1 (MQ=255)


      gCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAg                                                                                           >  2:455873/1‑101 (MQ=255)


      gCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAg                                                                                           <  1:3322406/101‑1 (MQ=255)


      gCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAg                                                                                           <  2:2055219/101‑1 (MQ=255)


       ccAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGa                                                                                          <  1:1048713/101‑1 (MQ=255)


        cAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGaa                                                                                         <  1:3988643/101‑1 (MQ=255)


        cAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGaa                                                                                         <  2:2256113/101‑1 (MQ=255)


        cAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGaa                                                                                         <  1:1749877/101‑1 (MQ=255)


         aGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAg                                                                                        <  1:3056166/101‑1 (MQ=255)


          gTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAgg                                                                                       >  1:3507598/1‑101 (MQ=255)


           tCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTCGCCCGGATGCGCCCATTGGAACGCATAGAAGGc                                                                                      >  1:4296374/1‑101 (MQ=255)


            cAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAGCGCCTGCCCCATCAAGAACAGCTTCTTCCGCGGATGCGCCCCTCGGAACGCATAGAAggtc                                                                                     >  2:847181/1‑99 (MQ=255)


             aaGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGccc                                                                                    >  2:2930741/1‑101 (MQ=255)


             aaGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGccc                                                                                    >  2:2860556/1‑101 (MQ=255)


             aaGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGccc                                                                                    <  1:63783/101‑1 (MQ=255)


             aaGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGccc                                                                                    >  2:280083/1‑101 (MQ=255)


               gggCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGc                                                                                  >  1:1068567/1‑101 (MQ=255)


                ggCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCa                                                                                 <  2:361809/101‑1 (MQ=255)


                ggCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCa                                                                                 <  1:1882342/101‑1 (MQ=255)


                 gCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAg                                                                                >  2:4066734/1‑101 (MQ=255)


                 gCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAg                                                                                <  2:1832764/101‑1 (MQ=255)


                   ccccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGt                                                                              <  1:3796135/101‑1 (MQ=255)


                   ccccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGt                                                                              >  2:3117533/1‑101 (MQ=255)


                   ccccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGt                                                                              >  1:3305503/1‑101 (MQ=255)


                    cccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGtt                                                                             >  1:3555976/1‑101 (MQ=255)


                      cTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTgg                                                                           <  1:2141367/101‑1 (MQ=255)


                      cTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTgg                                                                           >  1:4221432/1‑101 (MQ=255)


                       tGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCCGCTTCTTGCCCGGATGCGCCCATTGGAACGCCTAGAAGGCCCGCAGGTTggg                                                                          >  2:1564142/1‑100 (MQ=255)


                        gATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCg                                                                         >  1:362031/1‑101 (MQ=255)


                        gATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCg                                                                         >  1:2574046/1‑101 (MQ=255)


                        gATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCg                                                                         >  2:1692129/1‑101 (MQ=255)


                           gcgcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAt                                                                      >  2:2411143/1‑101 (MQ=255)


                            cgcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAtt                                                                     >  2:3436458/1‑101 (MQ=255)


                             gcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAttt                                                                    <  2:2545159/101‑1 (MQ=255)


                              cccATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTc                                                                   >  1:224604/1‑101 (MQ=255)


                               ccATTCGCGCCCCTCCGCGAACTCCTGCCCCATAAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTCGCGAATTTCt                                                                  <  1:4364121/101‑1 (MQ=255)


                                 aTTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCGCGGATGCGCCCATTGGCACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGc                                                                <  2:3355574/101‑1 (MQ=255)


                                  ttCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCa                                                               <  1:959486/101‑1 (MQ=255)


                                  ttCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCa                                                               <  2:3181544/101‑1 (MQ=255)


                                   tCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCaa                                                              >  2:2646622/1‑101 (MQ=255)


                                       gcCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCg                                                          >  1:3177294/1‑101 (MQ=255)


                                         cccTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGtc                                                        <  2:4857945/101‑1 (MQ=255)


                                         cccTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGtc                                                        <  1:4736661/101‑1 (MQ=255)


                                                ngAACNCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGc                                                 <  1:1278411/100‑1 (MQ=255)


                                                 gAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCa                                                >  1:4444475/1‑101 (MQ=255)


                                                 gAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCa                                                >  2:3565807/1‑101 (MQ=255)


                                                  aaCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCAt                                               >  1:4840817/1‑101 (MQ=255)


                                                  aaCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCAt                                               <  2:4124745/101‑1 (MQ=255)


                                                  aaCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCAt                                               <  1:1225144/101‑1 (MQ=255)


                                                    cTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCt                                             >  2:4958396/1‑101 (MQ=255)


                                                     tCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCtt                                            <  1:2198197/101‑1 (MQ=255)


                                                      ccTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTg                                           <  1:4468556/101‑1 (MQ=255)


                                                      ccTGCACCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGACTCCCGGCATCTTg                                           >  2:3338040/1‑101 (MQ=255)


                                                       cTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTCGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGc                                          <  1:2007027/101‑1 (MQ=255)


                                                         gCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGccc                                        >  2:3305045/1‑101 (MQ=255)


                                                         gCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGccc                                        >  2:764528/1‑101 (MQ=255)


                                                         gCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGccc                                        >  1:89294/1‑101 (MQ=255)


                                                          cttccgatctagcagcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCa                                       <  2:4512015/91‑1 (MQ=255)


                                                              aTGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCAt                                   <  1:2770682/101‑1 (MQ=255)


                                                              aTGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCAt                                   <  1:3202659/101‑1 (MQ=255)


                                                              aTGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCAt                                   <  2:3477160/101‑1 (MQ=255)


                                                              aTGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCAt                                   >  1:1861182/1‑101 (MQ=255)


                                                               tGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATc                                  >  1:2480559/1‑101 (MQ=255)


                                                               tGAAGACCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATc                                  <  1:817980/101‑1 (MQ=255)


                                                                gAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCg                                 >  2:872005/1‑101 (MQ=255)


                                                                gAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCACGCATcc                                 >  2:1982822/1‑100 (MQ=255)


                                                                  agagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAc                               >  2:2097951/1‑101 (MQ=255)


                                                                   gagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAcc                              <  2:5034737/101‑1 (MQ=255)


                                                                   gagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAcc                              <  1:4046920/101‑1 (MQ=255)


                                                                     gcagcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAcccc                            <  1:1015009/101‑1 (MQ=255)


                                                                      cagcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCt                           <  2:2724989/101‑1 (MQ=255)


                                                                        tcttcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTg                         <  2:4224500/100‑1 (MQ=255)


                                                                        gcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTg                         >  1:4974781/1‑101 (MQ=255)


                                                                         cttcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGc                        <  1:4615553/101‑1 (MQ=255)


                                                                          ttcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGcc                       >  1:1666878/1‑101 (MQ=255)


                                                                           tcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCg                      <  2:4632666/101‑1 (MQ=255)


                                                                           tcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCg                      >  2:1370221/1‑101 (MQ=255)


                                                                              tGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGa                   <  2:1204881/101‑1 (MQ=255)


                                                                              tGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGa                   >  1:2450385/1‑101 (MQ=255)


                                                                                cccGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAAc                 <  2:3776417/101‑1 (MQ=255)


                                                                                     aTGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCt            <  2:2190105/101‑1 (MQ=255)


                                                                                       gcgcCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtcg          <  2:4806683/101‑1 (MQ=255)


                                                                                         gcCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtcgtc        >  2:1354213/1‑101 (MQ=255)


                                                                                           ccATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTcgtcgt      >  1:2992542/1‑101 (MQ=255)


                                                                                            cATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTg     >  1:98677/1‑101 (MQ=255)


                                                                                            cATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTg     <  2:4426491/101‑1 (MQ=255)


                                                                                            cATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTg     >  1:4129134/1‑101 (MQ=255)


                                                                                             aTTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGa    >  2:2106908/1‑101 (MQ=255)


                                                                                              ttGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGAc   >  1:347090/1‑101 (MQ=255)


                                                                                               tGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACt  >  1:3101063/1‑101 (MQ=255)


GAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGTACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACT  >  NC_014034/1990864‑1991059

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG