Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,440 G→C 13.4% S2T (AGC→ACC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4400G→C13.4% 59.4 ‑1.6 ‑0.0194S2T (AGC→ACC) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/17):  ref base (84/84):  total (93/101)
Fisher's exact test strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.44e-01

Only 100 of 200 total aligned reads displayed.

CCGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACC  >  NC_014034/2155340‑2155540
                                                                                                    |                                                                                                    
  gccgACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCg                                                                                                    >  1:2968309/1‑101 (MQ=255)
  cccgACCCGGCCGCGGTCGCCCCCACCTTCTGATGCCCTCGGGCGCCGCCACCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGTGCg                                                                                                    <  2:1501195/100‑1 (MQ=255)
    cgACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCgct                                                                                                  >  1:1396027/1‑99 (MQ=255)
    cgACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGAt                                                                                                  >  2:4569651/1‑101 (MQ=255)
     gcccgggCCGCGGTCCCCCCCGCCTTCGGAGGCCCGGGGGGGCCTTCGCCGTCGCCGCCCCCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                 <  2:2997721/96‑1 (MQ=255)
     gccccGGCCGCGGTCGCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                 <  1:2299804/99‑1 (MQ=255)
      ccccGGCCGCGGCCCCCCTCCCCTTCTGATGCCCTGGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGc                                                                                                <  2:1907878/100‑1 (MQ=255)
       cccGGCCGCGGCCCCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCCCCCGCGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                               <  2:644311/101‑1 (MQ=255)
         cgcccccgGCCGCCCTCCCCTTCTGACACCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAg                                                                                             <  1:2474940/95‑1 (MQ=255)
            ccGCGGTCGCCCTCCCCTTCTGATGCCCCCGGGCCCCGTCACCGTCACCGCCCGCCGGGGGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgggg                                                                                          <  1:5018505/101‑1 (MQ=255)
               cgGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGNAGGNTCATGAGCGATGCAAGGGGCCt                                                                                       >  1:4516838/1‑101 (MQ=255)
               cgGTCCCCCTCCCCTTCTGATGCCCTCGGGCCCCGTCCCCGTCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                       <  2:2394577/101‑1 (MQ=255)
                ggTCGCCCCCCCCTTCTGATCCCCCCGGGCGCCGCCCCCGCCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                      <  1:3622755/101‑1 (MQ=255)
                 ggcccccTCCCCTTCTGATCCCCGCGGGCGCCGTCCCCGCCACCGCCCGCCGGGGGGCAAGCCGCGAAAGTGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                     <  2:656827/97‑1 (MQ=255)
                 gTCGCCCTCCCCTTCTGATGCCCTCGGGCGCCGCCACCGCCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                     <  1:2762913/101‑1 (MQ=255)
                 gTCGCCCTCCCCTTCTGATGCCCTCGGGCCCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                     <  2:3181617/101‑1 (MQ=255)
                     cccTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCACCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                 <  2:2764786/101‑1 (MQ=255)
                     cccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                 >  2:2333980/1‑101 (MQ=255)
                      ccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                >  2:4411295/1‑101 (MQ=255)
                        tCACCTGTTGATGCCCCGGGCCGCCGTCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCt                                                                              <  2:3093001/101‑1 (MQ=255)
                            cTTCGGATCCCCCGGGGCGCCGTCCCCGTCCCCCCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                          <  2:3328182/101‑1 (MQ=255)
                             ttcggacccccGCGGGCGCCGCCACCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCTTAGAGCtcgtcg                                                                         <  1:2733105/93‑1 (MQ=255)
                             ttCTGAGCCCCTGGGGCGCCGCCCCCGCCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtcg                                                                         <  2:3633903/101‑1 (MQ=255)
                              ttggcggCCCGCGGGCCCCGCCCCCGCCACCCCCCCCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                        <  2:2860112/95‑1 (MQ=255)
                              tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                        >  2:3446505/1‑101 (MQ=255)
                              tCTGATCCCCCGGGGCGCCGCCCCCGCCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                        <  1:10003/101‑1 (MQ=255)
                               cTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGAt                                                                       >  1:4865906/1‑101 (MQ=255)
                                 gATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                     >  1:3499027/1‑101 (MQ=255)
                                 gATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                     >  2:3589043/1‑101 (MQ=255)
                                 gATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                     >  1:1367330/1‑101 (MQ=255)
                                  gtGCCCTCGGGCGCCGTCCCCGCCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                    <  1:3997391/100‑1 (MQ=255)
                                  aTGCCCTCGGGCGCCGTCACCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGAGATGCAAGGGGCCTCGTCGAGCTCGCCGATGcc                                                                    >  1:4348606/1‑101 (MQ=255)
                                    gCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                  >  2:3924846/1‑101 (MQ=255)
                                    ccccGCGGCCCCCGCCCCCGTCCCCGCCCGCCGGGCGCCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                  <  2:4328034/100‑1 (MQ=255)
                                         ggggCCCCGCCCCCGCCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                             <  2:2552409/100‑1 (MQ=255)
                                                cgtcaccgtcacCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggggc                                                      >  1:1794183/1‑101 (MQ=255)
                                                 gtcaccgtcaccGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                     >  1:3099941/1‑101 (MQ=255)
                                                 gtcaccgtcaccGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                     >  2:817946/1‑101 (MQ=255)
                                                 gtcaccgtcaccGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCACCTggggcggggcg                                                     >  2:2471868/1‑100 (MQ=255)
                                                 gcccccgcccccgcccgcCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTCGGGCGGGGcc                                                     <  1:4365658/91‑1 (MQ=255)
                                                    accgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                  >  2:2189852/1‑101 (MQ=255)
                                                     ccgcccccgcccgcGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTca                                                 <  1:4234601/95‑1 (MQ=255)
                                                      cgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcac                                                >  1:373763/1‑101 (MQ=255)
                                                       gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                               >  1:302868/1‑101 (MQ=255)
                                                       gcccccccccgccGCGTGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                               <  1:1525600/94‑1 (MQ=255)
                                                         caccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcaccac                                             >  1:3177747/1‑101 (MQ=255)
                                                             ccccgccGGGCGACAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACTATg                                         <  2:5008132/100‑1 (MQ=255)
                                                              cccgccGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                        >  1:4632391/1‑101 (MQ=255)
                                                                cgccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcg                                      >  2:1778466/1‑101 (MQ=255)
                                                                 gccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcgg                                     <  2:3498757/101‑1 (MQ=255)
                                                                  ccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                    >  1:4565002/1‑101 (MQ=255)
                                                                  ccgGGCGCCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                    <  2:3248492/101‑1 (MQ=255)
                                                                    gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                  >  2:2899127/1‑101 (MQ=255)
                                                                    gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                  >  1:2236545/1‑101 (MQ=255)
                                                                        ggcAAGCCGCGAAAGGGCAGGATCATGAGCGATCCAAGGGGCCTCGTAGAGCTCGTCGATGACATCTGGGGCGGGGCCGTCACCACGATGGCGGGGgcgca                              >  2:929780/1‑100 (MQ=255)
                                                                         gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                             <  2:4516518/101‑1 (MQ=255)
                                                                         aaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                             <  1:1932877/99‑1 (MQ=255)
                                                                           aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                           <  2:3860977/101‑1 (MQ=255)
                                                                             gCCGCGAAAGGGCAGGATCATGAGCGATGCGAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCg                         <  1:3239468/101‑1 (MQ=255)
                                                                                 cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                     >  2:3030400/1‑101 (MQ=255)
                                                                                   aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                   >  2:4185257/1‑101 (MQ=255)
                                                                                    aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                  <  2:74944/101‑1 (MQ=255)
                                                                                    aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                  >  1:74954/1‑101 (MQ=255)
                                                                                      ggagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGa                >  1:862716/11‑101 (MQ=255)
                                                                                      ggagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGa                <  1:4822812/91‑1 (MQ=255)
                                                                                       ggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAt               >  1:2380904/1‑101 (MQ=255)
                                                                                       gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt               <  1:3399939/92‑1 (MQ=255)
                                                                                       gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt               <  2:4048788/92‑1 (MQ=255)
                                                                                        gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg              >  1:1468239/1‑101 (MQ=255)
                                                                                        gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg              >  1:1848175/1‑101 (MQ=255)
                                                                                        agaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtg              >  1:1260311/9‑101 (MQ=255)
                                                                                         cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt             >  1:584831/1‑101 (MQ=255)
                                                                                          aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgtg            <  2:2806749/95‑1 (MQ=255)
                                                                                          aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg            >  2:1095847/1‑101 (MQ=255)
                                                                                            aaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGc          >  1:1072106/5‑101 (MQ=255)
                                                                                             aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa         >  2:4846322/1‑101 (MQ=255)
                                                                                              tCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGGGGCGGCGCTCGCCGGGCGGTTGATGTGGCAt        >  2:4723196/1‑101 (MQ=255)
                                                                                              tCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAt        >  2:4351166/1‑101 (MQ=255)
                                                                                               cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt       <  2:1932518/101‑1 (MQ=255)
                                                                                               cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt       >  1:3875755/1‑101 (MQ=255)
                                                                                               cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt       <  1:2413376/101‑1 (MQ=255)
                                                                                               aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt       <  2:3150435/100‑1 (MQ=255)
                                                                                               aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt       <  2:4301066/100‑1 (MQ=255)
                                                                                               aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGGGGCAtt       >  2:1635381/2‑101 (MQ=255)
                                                                                                aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      <  1:1683617/101‑1 (MQ=255)
                                                                                                aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      <  2:1001096/101‑1 (MQ=255)
                                                                                                aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      <  1:2029270/101‑1 (MQ=255)
                                                                                                aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      <  1:3458932/101‑1 (MQ=255)
                                                                                                aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc      <  1:4615716/101‑1 (MQ=255)
                                                                                                aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc      <  2:1172435/101‑1 (MQ=255)
                                                                                                aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc      >  1:2464534/1‑101 (MQ=255)
                                                                                                aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc      <  2:1269636/101‑1 (MQ=255)
                                                                                                aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc      <  2:1890265/101‑1 (MQ=255)
                                                                                                 tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg     <  1:4293254/101‑1 (MQ=255)
                                                                                                 tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg     <  1:3490670/101‑1 (MQ=255)
                                                                                                 tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg     <  2:2218517/101‑1 (MQ=255)
                                                                                                  gAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGa    >  1:523585/1‑101 (MQ=255)
                                                                                                   aGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAc   <  2:373710/101‑1 (MQ=255)
                                                                                                    gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc  <  2:4249073/101‑1 (MQ=255)
                                                                                                    gCGATGCAAGGGGCCTCGGAGAGGTCGTCGAGGCCATCGGGGGCGGGGCCGCCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc  <  1:1345252/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CCGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACC  >  NC_014034/2155340‑2155540

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG