Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,564 C→G 39.2% R43R (CGC→CGG)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,5640C→G39.2% 0.0 ‑0.9 ‑1.3278R43R (CGC→CGG) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (63/46):  ref base (88/81):  total (151/127)
Fisher's exact test strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.51e-02

Only 100 of 311 total aligned reads displayed.

CGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTG  >  NC_014034/2155465‑2155663
                                                                                                   |                                                                                                   
 atcgATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGtt                                                                                                   >  2:664067/2‑101 (MQ=255)
   cgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGAAGCGCCGCTTcc                                                                                                 >  1:3188271/1‑101 (MQ=255)
   cgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTcc                                                                                                 >  1:169872/1‑101 (MQ=255)
    gATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGttttt                                                                                                >  1:2414548/1‑98 (MQ=255)
    gATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCt                                                                                                >  1:3721017/1‑101 (MQ=255)
    gATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCt                                                                                                >  1:645722/1‑101 (MQ=255)
     aTGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTg                                                                                               <  1:455021/101‑1 (MQ=255)
     aTGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTg                                                                                               <  2:3649947/101‑1 (MQ=255)
      tGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGGGCAAGCGCCGCTTCCTGa                                                                                              >  1:2659680/1‑101 (MQ=255)
           tCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGGGCGGGCGCGCAAGAGCCGCTTCCTGAGGcgc                                                                                         >  2:4544308/1‑101 (MQ=255)
            cTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCcgcg                                                                                        >  2:1542905/1‑101 (MQ=255)
            cTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCcgcg                                                                                        <  2:875961/101‑1 (MQ=255)
            cTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCcgcg                                                                                        <  2:2732689/101‑1 (MQ=255)
            cTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGATGCGGGCGCGCAAGCGCCGCTTCCTGAGCcgcg                                                                                        >  1:4346627/1‑101 (MQ=255)
               gggcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgc                                                                                     <  2:2496168/101‑1 (MQ=255)
               gggcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgc                                                                                     <  1:818048/101‑1 (MQ=255)
                   ggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgctgct                                                                                 <  1:3276908/101‑1 (MQ=255)
                   gagcccGTGACCACGATGGCGCGGGCGTTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgctgct                                                                                 <  2:4838878/97‑1 (MQ=255)
                       ccGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTgg                                                                             >  2:4317962/1‑101 (MQ=255)
                         gTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGa                                                                           >  1:465729/1‑101 (MQ=255)
                         gTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGa                                                                           >  1:3062001/1‑101 (MQ=255)
                          tCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAg                                                                          >  1:2494593/1‑101 (MQ=255)
                          gcaccacGATGGCGGCGGCGCGCTCCGGGCGGCTGATGGGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAg                                                                          <  2:755415/100‑1 (MQ=255)
                           cacgacGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGc                                                                         >  2:1620912/1‑101 (MQ=255)
                             cgacGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTg                                                                       <  2:1402292/99‑1 (MQ=255)
                              cacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGc                                                                      <  2:3829360/101‑1 (MQ=255)
                                cGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGccc                                                                    <  1:2763002/101‑1 (MQ=255)
                                 gATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCg                                                                   >  2:2532220/1‑101 (MQ=255)
                                  gtGGCGGCGGCGGTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGt                                                                  <  2:1147415/100‑1 (MQ=255)
                                    ggcggcggcGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTcg                                                                <  1:1095960/101‑1 (MQ=255)
                                     gcggcggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTggc                                                               <  1:1514508/101‑1 (MQ=255)
                                     gcggcggcgCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTcgc                                                               >  2:100434/1‑101 (MQ=255)
                                       ggcggcgCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCgg                                                             >  2:2797444/1‑101 (MQ=255)
                                           gcgcTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGgg                                                         >  1:80099/1‑101 (MQ=255)
                                           gcgcTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGgg                                                         >  1:1368484/1‑101 (MQ=255)
                                              cTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCAt                                                      <  1:4411614/101‑1 (MQ=255)
                                               ttgCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATg                                                     <  2:3071385/99‑1 (MQ=255)
                                               tggCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATg                                                     <  1:3558085/99‑1 (MQ=255)
                                               tggCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATg                                                     >  1:1459799/3‑101 (MQ=255)
                                               taggcGGGCGGCTGATGTGGCATTCGACCGGGGTTCGGGCGCGCAAGCCCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATg                                                     <  1:4723519/97‑1 (MQ=255)
                                               ccGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATg                                                     <  1:3446770/100‑1 (MQ=255)
                                                ggCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATgg                                                    >  2:2639875/2‑101 (MQ=255)
                                                cGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATgg                                                    <  1:2091770/101‑1 (MQ=255)
                                                 gcggggCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGc                                                   <  1:3353819/98‑1 (MQ=255)
                                                 gCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGc                                                   >  1:1146519/1‑101 (MQ=255)
                                                 gCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGc                                                   >  2:1883477/1‑101 (MQ=255)
                                                  ccGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCg                                                  >  2:3730564/1‑101 (MQ=255)
                                                   cGGGCGGCTGATGAGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCGGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGa                                                 >  2:3318192/1‑101 (MQ=255)
                                                   cGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGa                                                 >  2:3037776/1‑101 (MQ=255)
                                                    gggCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGat                                                <  2:2249429/101‑1 (MQ=255)
                                                       cgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCCGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcat                                             <  2:1193076/98‑1 (MQ=255)
                                                        ggcTAATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcatc                                            <  1:1917112/101‑1 (MQ=255)
                                                        gccggATGTGGAATTCGACCGAGGTGCGGGCGCGGCGACGCCGTTTTTTCGGACGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCTatcatc                                            <  2:1184268/97‑1 (MQ=255)
                                                        gccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcatc                                            >  1:4174251/3‑101 (MQ=255)
                                                         gcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCg                                           >  1:2549874/1‑101 (MQ=255)
                                                         ccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCg                                           <  2:3191278/100‑1 (MQ=255)
                                                         ccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCg                                           >  1:4006041/2‑101 (MQ=255)
                                                          cTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCgg                                          <  1:1780815/101‑1 (MQ=255)
                                                            gATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCg                                        >  2:3443905/1‑101 (MQ=255)
                                                            gATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCg                                        >  2:1102618/1‑101 (MQ=255)
                                                            gATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGGCGGCAAGGCgcccttccggg                                        >  2:4031215/1‑91 (MQ=255)
                                                               gtgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAgg                                     <  1:1278794/101‑1 (MQ=255)
                                                               gtgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAgg                                     >  1:4096304/1‑101 (MQ=255)
                                                                tgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGc                                    <  2:3119968/101‑1 (MQ=255)
                                                                 ggCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCg                                   >  1:2617636/1‑101 (MQ=255)
                                                                  gCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCgg                                  >  1:4565736/1‑101 (MQ=255)
                                                                   cATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTCTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGt                                 <  1:3680288/101‑1 (MQ=255)
                                                                    aTTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTg                                >  2:1378967/1‑101 (MQ=255)
                                                                     ttCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTgg                               >  1:681137/1‑101 (MQ=255)
                                                                     ttCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTgg                               <  2:3554078/101‑1 (MQ=255)
                                                                     ttCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTgg                               <  2:1352836/101‑1 (MQ=255)
                                                                      tCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggg                              >  2:1276772/1‑100 (MQ=255)
                                                                      tCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggc                              <  1:1598773/101‑1 (MQ=255)
                                                                        gACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggcgg                            <  2:4148401/101‑1 (MQ=255)
                                                                        gACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGGggcgg                            >  1:4836969/1‑101 (MQ=255)
                                                                         aCCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggcggc                           <  2:4565415/101‑1 (MQ=255)
                                                                         aCCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggcggc                           >  2:3927268/1‑101 (MQ=255)
                                                                          ccGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGGggggggt                          >  1:3940222/1‑99 (MQ=255)
                                                                           cGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCtt                         <  2:615915/101‑1 (MQ=255)
                                                                           cGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCtt                         <  2:2074346/101‑1 (MQ=255)
                                                                           cGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCtt                         >  2:1935586/1‑101 (MQ=255)
                                                                            gAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                        >  2:4990325/1‑101 (MQ=255)
                                                                            gAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                        >  2:2195880/1‑101 (MQ=255)
                                                                             aGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAt                       >  1:2154302/1‑101 (MQ=255)
                                                                              ggTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAtt                      >  2:2951013/1‑101 (MQ=255)
                                                                              ggTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAtt                      <  1:2349258/101‑1 (MQ=255)
                                                                              ggTGCGGGCCCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAtt                      <  1:4808277/101‑1 (MQ=255)
                                                                               gTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAttt                     <  1:1021106/101‑1 (MQ=255)
                                                                               gTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAttt                     <  1:3645116/101‑1 (MQ=255)
                                                                               gTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGGGGCGGCTTTttt                     >  1:4474098/1‑101 (MQ=255)
                                                                               gTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAttt                     <  2:3720739/101‑1 (MQ=255)
                                                                                 gggggCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCg                   <  2:2143908/99‑1 (MQ=255)
                                                                                     gcgcgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCt               <  2:3399039/101‑1 (MQ=255)
                                                                                         gcAAGCGCCGCTTCCTGAGCCGCGAGCTTCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGAGGCGGCTTATTTCGAGCTCgcc           >  1:1329218/1‑99 (MQ=255)
                                                                                         gcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGc           <  1:2196035/101‑1 (MQ=255)
                                                                                         gcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGc           >  2:2441127/1‑101 (MQ=255)
                                                                                            aGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCgg        <  1:3012073/101‑1 (MQ=255)
                                                                                               gccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGAcc     >  2:1773943/1‑101 (MQ=255)
                                                                                                ccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGAACg    >  2:4740641/1‑101 (MQ=255)
                                                                                                  gcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTg  <  1:1390891/101‑1 (MQ=255)
                                                                                                   |                                                                                                   
CGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTG  >  NC_014034/2155465‑2155663

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG