Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,693 T→C 27.5% Y86Y (TAT→TAC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,6930T→C27.5% 229.4 ‑3.3 ‑0.7298Y86Y (TAT→TAC) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (51/31):  ref base (105/111):  total (156/142)
Fisher's exact test strand distribution p-value = 3.85e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.00e-01

Only 100 of 307 total aligned reads displayed.

TGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCT  >  NC_014034/2155593‑2155792
                                                                                                    |                                                                                                   
 gCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtc                                                                                                    >  2:3747170/1‑101 (MQ=255)
    ggtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCg                                                                                                 >  1:543347/11‑101 (MQ=255)
    ggtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCg                                                                                                 >  2:3021954/11‑101 (MQ=255)
    ggtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCg                                                                                                 >  2:293766/11‑101 (MQ=255)
    cGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCg                                                                                                 <  1:1795234/101‑1 (MQ=255)
     gtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCgg                                                                                                >  2:2670381/10‑101 (MQ=255)
      tggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGGGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGc                                                                                               >  2:1344039/9‑101 (MQ=255)
       ggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGcc                                                                                              >  1:2617969/8‑101 (MQ=255)
       ggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGcc                                                                                              <  1:1446865/94‑1 (MQ=255)
        gccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGccc                                                                                             >  1:1674250/7‑101 (MQ=255)
         cgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcg                                                                                            <  2:1107839/101‑1 (MQ=255)
         cgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcg                                                                                            <  1:4741053/101‑1 (MQ=255)
         ccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcg                                                                                            <  1:3444169/96‑1 (MQ=255)
          ggTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCCCCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcgc                                                                                           <  1:4544628/101‑1 (MQ=255)
           gtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgcg                                                                                          >  2:1065228/4‑101 (MQ=255)
             gggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgcgcg                                                                                        >  1:4599592/2‑101 (MQ=255)
              ggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCgcgg                                                                                       >  1:57581/1‑101 (MQ=255)
                gaTGGGGAGCACCGGGGAGGGGGTGGGGGGTTATTTCGAGCTCGGCCGCGCCGTGTCCCCCGGGGCGGTCTCACCGCTCGCCTATCTCGGCCCGCgcggcg                                                                                     <  2:4620869/100‑1 (MQ=255)
                cATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCgcggcg                                                                                     >  1:643329/1‑101 (MQ=255)
                 aTGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGgggc                                                                                    >  2:2435056/1‑101 (MQ=255)
                   ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCgcggggatc                                                                                  >  2:657213/1‑96 (MQ=255)
                   ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGccg                                                                                  <  2:4329140/101‑2 (MQ=255)
                   ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGccg                                                                                  <  2:4650178/101‑2 (MQ=255)
                   ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGccc                                                                                  >  1:1062479/1‑101 (MQ=255)
                    gCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGccga                                                                                 >  2:3300032/1‑99 (MQ=255)
                     cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAg                                                                                <  1:717314/101‑1 (MQ=255)
                     cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGACCAg                                                                                <  1:1025173/101‑1 (MQ=255)
                     cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAg                                                                                >  1:1534461/1‑101 (MQ=255)
                      gATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAgg                                                                               <  1:1621044/101‑1 (MQ=255)
                       atcatcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAggg                                                                              >  1:2137585/1‑100 (MQ=255)
                       atcatcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCGCGCTCGCCTACCTCGGCCCGGGCGGGGCCGAggg                                                                              >  1:4339367/1‑100 (MQ=255)
                       atcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAggg                                                                              >  1:242906/1‑100 (MQ=255)
                          atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgc                                                                           >  1:2826242/1‑101 (MQ=255)
                            cGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgc                                                                         <  1:2276021/101‑5 (MQ=255)
                             ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCCGGTGgcgcc                                                                        >  2:1806489/1‑100 (MQ=255)
                             ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                                                        >  1:5045421/1‑101 (MQ=255)
                             ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCACt                                                                        >  2:4257648/1‑101 (MQ=255)
                              gCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTg                                                                       <  2:4832633/101‑1 (MQ=255)
                              gCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTg                                                                       <  1:3069288/101‑1 (MQ=255)
                              gCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTg                                                                       <  2:4536005/101‑1 (MQ=255)
                               cGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGa                                                                      >  1:1856721/1‑101 (MQ=255)
                                 aGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatg                                                                    <  2:2517934/101‑3 (MQ=255)
                                 aGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAcc                                                                    >  1:1893611/1‑101 (MQ=255)
                                  ggCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAccc                                                                   <  1:2532401/101‑1 (MQ=255)
                                   gCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCg                                                                  <  1:4213065/101‑1 (MQ=255)
                                    cGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCgg                                                                 <  2:4171771/101‑1 (MQ=255)
                                    cGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCgg                                                                 <  2:4664861/101‑1 (MQ=255)
                                     ggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagga                                                                <  2:2318369/101‑7 (MQ=255)
                                     ggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagga                                                                >  2:218375/1‑95 (MQ=255)
                                     ggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagga                                                                >  2:194606/1‑95 (MQ=255)
                                     ggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagga                                                                >  1:4003604/1‑95 (MQ=255)
                                      gTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGGCCCgggc                                                               >  2:4065156/1‑100 (MQ=255)
                                       tGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtc                                                              >  2:3349506/1‑101 (MQ=255)
                                        ggcggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGGCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGACCAGGGGGCGCTGACCCGGGtct                                                             >  2:4754951/1‑101 (MQ=255)
                                          cggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttc                                                           <  2:3061763/101‑1 (MQ=255)
                                             cTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCttt                                                        >  2:2806160/1‑101 (MQ=255)
                                              ttATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTc                                                       <  1:4082054/101‑1 (MQ=255)
                                              ttATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTc                                                       <  1:1400850/101‑1 (MQ=255)
                                                aTTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcac                                                     >  1:4844634/1‑101 (MQ=255)
                                                aTTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcac                                                     <  1:1149620/101‑1 (MQ=255)
                                                 tttCGAGCTCGGACGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcaca                                                    >  1:2737876/1‑101 (MQ=255)
                                                  ttCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaa                                                   >  1:2467923/1‑101 (MQ=255)
                                                     gAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCAcaacaa                                                >  2:1454750/1‑101 (MQ=255)
                                                      aGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAg                                               <  1:848923/101‑1 (MQ=255)
                                                       gCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAgg                                              <  2:3550330/101‑1 (MQ=255)
                                                         tCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAg                                            <  1:4990655/101‑1 (MQ=255)
                                                          cGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGt                                           <  1:286953/101‑1 (MQ=255)
                                                            gCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCg                                         <  2:383749/101‑1 (MQ=255)
                                                            gCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCg                                         <  1:1179878/101‑1 (MQ=255)
                                                             ccGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGt                                        >  2:511140/1‑101 (MQ=255)
                                                              cGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTg                                       >  2:2083009/1‑101 (MQ=255)
                                                               ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTga                                      >  1:754513/1‑101 (MQ=255)
                                                               ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTga                                      <  2:4891037/101‑1 (MQ=255)
                                                                gACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgag                                     <  2:169856/101‑1 (MQ=255)
                                                                 aCCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgaga                                    <  1:3952493/101‑1 (MQ=255)
                                                                  ccGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGAt                                   <  1:3747448/101‑1 (MQ=255)
                                                                   cGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATg                                  <  1:3927560/101‑1 (MQ=255)
                                                                     tGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgac                                >  1:1206286/1‑101 (MQ=255)
                                                                     tGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgac                                <  1:2938204/101‑1 (MQ=255)
                                                                      gACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacg                               <  1:945247/101‑1 (MQ=255)
                                                                       accaccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacga                              >  1:4874466/1‑101 (MQ=255)
                                                                        ccaccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacgac                             <  2:3622408/101‑1 (MQ=255)
                                                                          accGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAg                           <  1:209422/101‑1 (MQ=255)
                                                                            cGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGAc                         <  2:1505722/101‑1 (MQ=255)
                                                                            cGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGAc                         <  1:1912696/101‑1 (MQ=255)
                                                                                  cgtcgCAACGCTCGCCTATCTCGGCCCGCGCGGCTCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCgg                   >  1:980011/1‑101 (MQ=255)
                                                                                   gtcgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCggg                  >  2:4963885/1‑101 (MQ=255)
                                                                                     cgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGcc                <  1:3688424/101‑1 (MQ=255)
                                                                                       cAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGcgg              >  1:3148888/1‑100 (MQ=255)
                                                                                         aCGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCgcgc            <  1:3553917/101‑1 (MQ=255)
                                                                                           gCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCCTGAGATGACGACAGACTGGCGGGCCGCGCAg          <  1:4477196/101‑1 (MQ=255)
                                                                                              cGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTg       <  2:1404211/101‑1 (MQ=255)
                                                                                               gCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCcgccgggcggc      >  1:3837256/1‑93 (MQ=255)
                                                                                                 cTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGGGCGCCGCTGGgg    >  1:1305746/1‑101 (MQ=255)
                                                                                                 cTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCGGcgtcgg    >  2:753403/1‑96 (MQ=255)
                                                                                                   aTCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACGAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCt  <  2:4371175/101‑1 (MQ=255)
                                                                                                   aTCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCGGCCGCgggc  >  1:2704559/1‑99 (MQ=255)
                                                                                                   aTCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCCGCGGCgggc  >  2:3911463/1‑99 (MQ=255)
                                                                                                   aTCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGAGCAGCTggcgcg  >  2:1271889/1‑96 (MQ=255)
                                                                                                   aTCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCCCGCGGCCGCGGcg  >  1:4913391/1‑100 (MQ=255)
                                                                                                    |                                                                                                   
TGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCT  >  NC_014034/2155593‑2155792

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG