Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,186,012	G→A	100%	intergenic (+285/‑220)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,186,012	0	G→A	100.0%	174.6	51	intergenic (+285/‑220)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (28/23):  ref base (0/0):  total (28/23)

ACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCC...GC.CGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGAA  >  NC_014034/2185920‑2186109                                                                                                      |                                                                                                  aCGCCGACCCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc                                                                                            <  2:4712730/101‑1 (MQ=2)      gAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGGCGCCGCCGGG.G.AAGGCCGAGGCGGAAAAcccccccgc                                                                                       >  1:638168/1‑96 (MQ=2)         ccccccccTTCCACCTTGGCCAAAAGACCCCGGGGGCCGGGGGCCGTA..GC.CCC...TCGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCac                                                                                    <  2:4195431/97‑1 (MQ=2)                 tttCACCTGGGCTCAAATACCCCGGGGGGCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACcgaccgac                                                                            <  1:1354669/101‑1 (MQ=9)                     cccTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGc                                                                        <  2:2483931/100‑1 (MQ=9)                     cccTTGGCTCAAATACCCGGGGGGCCGGGGGGCGGACCC..CCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGc                                                                        <  2:4581361/100‑1 (MQ=9)                      ccTTGGCTCAAATACCCCGGGGGTCCGGGGGGGGACCC..CCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCg                                                                       <  2:2305330/101‑1 (MQ=9)                        ttGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGAc                                                                     <  1:2471680/101‑1 (MQ=14)                                   atCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGGCGCCCCCGG..CCAAGCCCCAGGCGAAAAACCCCAGCGCCCCCCCCCCACCTGCGACGGCCCGGcgcg                                                          >  2:4058630/1‑101 (MQ=2)                                     cccGGGGGGCCGGGGGCGGA..G..CCCCCGGG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCgcgc                                                         <  1:737388/101‑1 (MQ=12)                                        gggggTCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaaa                                                     <  1:1619847/101‑1 (MQ=18)                                                     ggcggacccccc...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcagg                                          <  2:631719/92‑1 (MQ=21)                                                       cggagccccc...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggca                                        <  1:2714778/94‑1 (MQ=21)                                                                                cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc                     >  1:2619064/10‑101 (MQ=255)                                                                                cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc                     >  1:77378/10‑101 (MQ=255)                                                                                cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc                     <  2:1261239/92‑1 (MQ=255)                                                                                 ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc                    >  1:1442725/9‑101 (MQ=255)                                                                                 ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc                    >  1:4896283/9‑101 (MQ=255)                                                                                   cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                  >  1:2288401/7‑101 (MQ=255)                                                                                    gggcaagccccGAGGCGAAACACGCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 <  2:1147350/92‑1 (MQ=255)                                                                                    gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCTTTGACCCTTc                 >  2:229589/6‑101 (MQ=255)                                                                                    gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 <  1:3881146/96‑1 (MQ=255)                                                                                    gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 <  2:2825219/96‑1 (MQ=255)                                                                                    gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 >  2:4496888/6‑101 (MQ=255)                                                                                     ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg                <  1:1728182/97‑1 (MQ=255)                                                                                     ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACGTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCCTCg                >  2:867851/5‑101 (MQ=255)                                                                                      gcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCgg               <  2:1935390/98‑1 (MQ=255)                                                                                      gcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCgg               >  2:3629111/4‑101 (MQ=255)                                                                                      gcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCgg               <  1:4960547/98‑1 (MQ=255)                                                                                       caaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGa              >  1:1134741/3‑101 (MQ=255)                                                                                        aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc             >  2:1056730/2‑101 (MQ=255)                                                                                        aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc             <  1:2421863/100‑1 (MQ=255)                                                                                        aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc             >  1:3985588/2‑101 (MQ=255)                                                                                         aaGCCCGAGGCGAAACCCCCCAGCGCCACCGCCCGGCTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGGcc            <  2:2176489/101‑1 (MQ=255)                                                                                         aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc            <  1:3292523/101‑1 (MQ=255)                                                                                         aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc            >  1:3277916/1‑101 (MQ=255)                                                                                         aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc            >  1:3329145/1‑101 (MQ=255)                                                                                          agcccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCg           <  2:868764/98‑1 (MQ=255)                                                                                          agcccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCg           >  1:3595973/4‑101 (MQ=255)                                                                                             ccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGt        <  2:4454473/101‑1 (MQ=255)                                                                                             ccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGt        >  2:2548941/1‑101 (MQ=255)                                                                                             ccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGt        >  2:317200/1‑101 (MQ=255)                                                                                              cGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGtt       >  2:4395223/1‑101 (MQ=255)                                                                                              cGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGtt       >  1:2972218/1‑101 (MQ=255)                                                                                              cGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGtt       >  1:1499736/1‑101 (MQ=255)                                                                                              cGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGtt       >  2:904031/1‑101 (MQ=255)                                                                                              cGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGtt       >  2:3961692/1‑101 (MQ=255)                                                                                              cGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGtt       <  1:4025534/101‑1 (MQ=255)                                                                                                aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg     >  1:3772125/1‑101 (MQ=255)                                                                                                aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg     >  2:153968/1‑101 (MQ=255)                                                                                                 ggCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTggg    >  1:2981914/1‑101 (MQ=255)                                                                                                 ggCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTggg    <  1:3774403/101‑1 (MQ=255)                                                                                                 ggCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTggg    >  1:4450454/1‑101 (MQ=255)                                                                                                 ggCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTggg    <  1:470586/101‑1 (MQ=255)                                                                                                  gCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGa   >  1:4759942/1‑101 (MQ=255)                                                                                                   cGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGaa  >  2:39429/1‑101 (MQ=255)                                                                                                      |                                                                                                  ACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCC...GC.CGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGAA  >  NC_014034/2185920‑2186109 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG