Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 221,940 A→G 5.8% H239H (CAT→CAC RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034221,9400A→G 5.8% 17.4 ‑1.5 ‑0.6188H239H (CAT→CACRCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/3):  ref base (87/90):  total (95/93)
Fisher's exact test strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.24e-01

Only 100 of 219 total aligned reads displayed.

CACGGTCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGA  >  NC_014034/221841‑222039
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cccGGTCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGAt                                                                                                    <  2:4471038/99‑1 (MQ=255)
cccGGTCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGAt                                                                                                    <  2:3227967/99‑1 (MQ=255)
 acGGGCGAGCGGGTTTCGCCGGTGCCCGACGCCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGGGATCATCGACGGTGGGCGTCGGATg                                                                                                   <  2:4106941/101‑1 (MQ=255)
    gTCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGt                                                                                                >  2:4146334/1‑101 (MQ=255)
      cGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTgg                                                                                              <  1:1248340/101‑1 (MQ=255)
      cGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTgg                                                                                              >  2:2705006/1‑101 (MQ=255)
       gAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTggg                                                                                             <  1:4278883/101‑1 (MQ=255)
       gAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGGGGGCGTCGGATGCGTggg                                                                                             >  2:3969058/1‑101 (MQ=255)
        aGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGc                                                                                            >  2:88906/1‑101 (MQ=255)
           cGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCtcg                                                                                         <  2:434375/101‑1 (MQ=255)
            ggTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCCCATCGACGGTGGGCGTCGGGTGGGTGGGCtcgg                                                                                        >  1:4343487/1‑101 (MQ=255)
             gTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCtcggt                                                                                       >  2:1191249/1‑101 (MQ=255)
              tttCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCtcggtc                                                                                      <  1:3575717/101‑1 (MQ=255)
              tttCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCtcggtc                                                                                      <  1:2248286/101‑1 (MQ=255)
                tCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCtcggtcgg                                                                                    <  2:3244220/101‑1 (MQ=255)
                 cGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCgga                                                                                   <  2:2027219/101‑1 (MQ=255)
                  gCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggat                                                                                  >  2:3533109/1‑101 (MQ=255)
                  gCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggat                                                                                  <  1:2008377/101‑1 (MQ=255)
                  gCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGGGGGCGTCGGATGCGTGGGCTCGGTCggat                                                                                  >  2:3078627/1‑101 (MQ=255)
                  cccGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggat                                                                                  <  2:1457259/100‑1 (MQ=255)
                    cGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGGGGGCGTCGGATGCGTGGGCTCGGTCggatcg                                                                                <  1:4537028/101‑1 (MQ=255)
                     ggTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcgg                                                                               >  2:4720356/1‑101 (MQ=255)
                     ggTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGCGGGCGTCGGATGCGGGGGCCCGGTCggatcgg                                                                               >  2:1468594/1‑101 (MQ=255)
                      gTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggt                                                                              >  1:470096/1‑101 (MQ=255)
                       tGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtc                                                                             <  2:605323/101‑1 (MQ=255)
                        gCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcg                                                                            >  1:622820/1‑101 (MQ=255)
                        gCCCGACACCGTCGCCGAGGCTTCGGGGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGCGCCCGGTCggatcggtcg                                                                            >  2:1895466/1‑101 (MQ=255)
                            gACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGGGGGCTCGGTCggatcggtcgggtc                                                                        >  2:4701216/1‑101 (MQ=255)
                             acacCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtcc                                                                       <  2:664049/101‑1 (MQ=255)
                                    cGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtggggt                                                                >  1:1831086/1‑99 (MQ=255)
                                     gCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatc                                                               <  2:2754476/101‑1 (MQ=255)
                                        gAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggt                                                            <  2:2657097/101‑1 (MQ=255)
                                        gAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggt                                                            >  1:2002773/1‑101 (MQ=255)
                                         aGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGGGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtggggtcggtc                                                           >  1:3037129/1‑101 (MQ=255)
                                            cTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcggg                                                        >  2:4639133/1‑101 (MQ=255)
                                              tCGGTGTTGGGTGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcgggcgggtccgtgggatcggtcgggtc                                                      <  2:156938/101‑1 (MQ=255)
                                               cGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggtcc                                                     >  1:4428982/1‑101 (MQ=255)
                                               cGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggtcc                                                     <  1:3016054/101‑1 (MQ=255)
                                                 gtgtTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggtccgt                                                   <  2:3196840/101‑1 (MQ=255)
                                                 gtgtTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcggggccgtgggatcggtcgggtccgt                                                   >  1:3411082/1‑101 (MQ=255)
                                                  tgtTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggtccgtg                                                  >  1:2454752/1‑101 (MQ=255)
                                                   gtTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGgatcggtcgggtccgtgggaccggtcggggccgggg                                                 >  1:691933/1‑101 (MQ=255)
                                                    ttGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGatcggtcgggtccgtgggatcggtcgggtccgtggg                                                >  1:4638630/1‑101 (MQ=255)
                                                    ttGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGatcggtcgggtccgtgggatcggtcgggtccgtggg                                                >  1:2319833/1‑101 (MQ=255)
                                                     tggtggTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGatcggtcgggtccgtgggatcggtcgggtccgcgggg                                               >  2:1784458/1‑100 (MQ=255)
                                                       gtggtCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCggtcgggtccgtgggatcggtcgggtccgtgggatc                                             <  2:3697826/101‑1 (MQ=255)
                                                         ggtCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGAGGCGTGGGCTCGGTCGGATCGGtcgggtccgtgggatcggtcgggtccgtgggatcgg                                           <  1:2083003/101‑1 (MQ=255)
                                                         ggtCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGGCGGATGCGTGGGCTCGGTCGGATCGGtcgggtccgtgggatcggtcgggtccgtgggatcgg                                           >  2:2214221/1‑101 (MQ=255)
                                                            cAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGggtccgtgggatcggtcggggccgtgggatcggtcg                                        >  2:2802623/1‑101 (MQ=255)
                                                            cAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGggtccgtgggatcgggcgggtccgtggggtcggtcg                                        >  2:2374426/1‑101 (MQ=255)
                                                            cAGCGTCCCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGgggccgtgggatcggggggggccgtgggagcggtcg                                        >  2:2900789/1‑101 (MQ=255)
                                                             aGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGgtccgtgggatcggtcgggtccgtgggatcggtcgg                                       <  2:3851115/101‑1 (MQ=255)
                                                             aGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGgtccgtgggatcggtcgggtccgtgggatcggtcgg                                       <  2:4505861/101‑1 (MQ=255)
                                                               cGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGAt                                     >  1:1030726/1‑101 (MQ=255)
                                                                gTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATc                                    <  2:819951/101‑1 (MQ=255)
                                                                gTCGCCGGATCGCGCTCATCGACGGTGGGCGCCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTTGGGTCCGTGGGTTCGGTCGGGTc                                    >  1:2679678/1‑101 (MQ=37)
                                                                 tCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATcc                                   <  1:4146651/101‑1 (MQ=255)
                                                                  cGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCg                                  >  1:2898827/1‑101 (MQ=255)
                                                                   gCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGt                                 <  2:2191448/101‑1 (MQ=255)
                                                                    ccGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTg                                >  1:2768716/1‑101 (MQ=255)
                                                                     cGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTgg                               >  1:212985/1‑101 (MQ=255)
                                                                     cGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTgg                               >  2:4575958/1‑101 (MQ=255)
                                                                      ggATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTggg                              >  1:4045183/1‑101 (MQ=255)
                                                                       gATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGa                             <  1:1594880/101‑1 (MQ=255)
                                                                         tCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                           <  1:4821728/101‑1 (MQ=255)
                                                                         tCGCGCTCATCGACGGTGGGCGGCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                           <  1:528603/101‑1 (MQ=255)
                                                                          cgcgCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcg                          <  1:719765/101‑1 (MQ=255)
                                                                          cgcgCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGCGCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCCTGCGGGTCCGTCGGAccg                          >  2:858960/1‑101 (MQ=25)
                                                                           gcgcTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcgg                         >  2:1598434/1‑101 (MQ=255)
                                                                            cgcTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggt                        <  2:4428663/101‑1 (MQ=255)
                                                                             gcTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                       <  2:2566223/101‑1 (MQ=255)
                                                                              cTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtcg                      <  1:1433662/101‑1 (MQ=255)
                                                                               tCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtcgg                     >  2:4029109/1‑101 (MQ=255)
                                                                                cATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGGCggg                    >  2:1688485/1‑101 (MQ=255)
                                                                                 aTCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGt                   <  1:4867085/101‑1 (MQ=255)
                                                                                  tCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTc                  <  2:2158529/101‑1 (MQ=255)
                                                                                   cGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTcc                 >  1:2565137/1‑101 (MQ=255)
                                                                                    gACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGACGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCg                >  2:4204723/1‑101 (MQ=255)
                                                                                     gtggggtcggTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCTGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGt               >  2:4904909/10‑101 (MQ=11)
                                                                                     aCGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGt               >  2:3025185/1‑101 (MQ=255)
                                                                                      tgggatcggTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGtc              <  1:3231168/93‑2 (MQ=1)
                                                                                      cGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCggg              >  2:1027618/1‑99 (MQ=255)
                                                                                       gggggcggTCGGTTCCGTGGGGTCGGGGGGGTCGGTGGGGTCGGTGGGATCGGTGGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGtcg             <  2:1831133/94‑3 (MQ=2)
                                                                                       gggatcggTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGtcg             >  2:3460898/8‑99 (MQ=11)
                                                                                       ggTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg             >  2:336155/1‑101 (MQ=255)
                                                                                       ggTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGGCCGTgg             >  2:2460259/1‑101 (MQ=255)
                                                                                         tGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGCCCGTgcat           >  1:1523878/1‑98 (MQ=255)
                                                                                           tcggTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCgccgggtc         >  2:3348417/4‑94 (MQ=11)
                                                                                            gCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcg        <  1:1895603/101‑1 (MQ=255)
                                                                                            cggTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTtggggcc        >  1:843734/3‑97 (MQ=11)
                                                                                            ccggctgATGCGTCTGATCCGTGGGATCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcg        <  2:4414395/95‑1 (MQ=11)
                                                                                             cGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGCCCGTgcatcaag       <  2:1523748/101‑8 (MQ=255)
                                                                                              gTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGGCCGTCGGGtccgt      >  1:927309/1‑101 (MQ=11)
                                                                                              gTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggt      >  1:2181092/1‑101 (MQ=255)
                                                                                               tCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATAGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGtccgtg     <  1:3836763/101‑2 (MQ=11)
                                                                                               tCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc     >  1:3150941/1‑101 (MQ=255)
                                                                                               tCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc     >  1:3720793/1‑101 (MQ=255)
                                                                                                cGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcg    <  1:3350266/101‑1 (MQ=255)
                                                                                                 ggATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGtcggtcgg   >  2:1120016/1‑101 (MQ=255)
                                                                                                 ggATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcgggcgg   >  1:988919/1‑101 (MQ=255)
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CACGGTCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGA  >  NC_014034/221841‑222039

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG