Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,316 A→C 6.2% intergenic (‑112/‑193) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3160A→C 6.2% 36.7 ‑0.2 ‑0.2259intergenic (‑112/‑193)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (9/7):  ref base (121/122):  total (130/129)
Fisher's exact test strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.50e-01

Only 100 of 277 total aligned reads displayed.

CATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGAC  >  NC_014034/2249217‑2249414
                                                                                                     |                                                                                                            
cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                                              <  1:3882108/101‑1 (MQ=255)
cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                                              <  2:2184046/101‑1 (MQ=255)
cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                                              >  1:1789917/1‑101 (MQ=255)
 aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                                             >  1:1306822/1‑101 (MQ=255)
  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                            <  2:321150/101‑1 (MQ=255)
  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                            <  2:1505130/101‑1 (MQ=255)
  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                            <  2:56748/101‑1 (MQ=255)
   tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                                           <  2:2518519/101‑1 (MQ=255)
    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                                          >  1:3690304/1‑101 (MQ=255)
    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                                          <  1:4453268/101‑1 (MQ=255)
     gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCCCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                                         <  1:2957079/101‑1 (MQ=255)
     gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                                         >  1:2653259/1‑101 (MQ=255)
      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGCCCCGTGCCGGGACT.CCCCCGGGCTACTTGAGGCAAg                                                                                                        >  2:3188046/1‑101 (MQ=255)
      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGAGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                                        <  2:3570259/101‑1 (MQ=255)
       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                                       <  2:1152044/101‑1 (MQ=255)
       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                                       >  1:4682471/1‑101 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTTAGGCAAGATg                                                                                                     <  2:4976744/101‑1 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                     <  2:4414191/101‑1 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                     <  2:3509049/101‑1 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                     >  2:3980772/1‑101 (MQ=255)
          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                    <  2:4610609/101‑1 (MQ=255)
          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                    >  2:4564001/1‑101 (MQ=255)
             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                                              >  1:194776/1‑101 (MQ=255)
               gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                            <  2:4684849/101‑1 (MQ=255)
                 tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..gg                                                                                        >  2:4015715/1‑101 (MQ=255)
                 tGCACAGATACCGCAGATTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..gg                                                                                        >  2:2215056/1‑101 (MQ=255)
                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.t                                                                                     <  1:1417376/101‑1 (MQ=255)
                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.t                                                                                     <  2:1330604/101‑1 (MQ=255)
                    acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.tt                                                                                    <  1:3126344/101‑1 (MQ=255)
                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.ttt                                                                                   <  1:4517889/101‑1 (MQ=255)
                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.ttt                                                                                   <  2:1075587/101‑1 (MQ=255)
                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTc                                                                                  <  1:725887/101‑1 (MQ=255)
                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTc                                                                                  <  1:3049482/101‑1 (MQ=255)
                       gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCa                                                                                 >  1:3914683/1‑101 (MQ=255)
                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCAt                                                                                >  2:4133258/1‑101 (MQ=255)
                          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCt                                                                              >  2:4356992/1‑101 (MQ=255)
                           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCtt                                                                             <  1:1092739/101‑1 (MQ=255)
                           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCtt                                                                             <  1:473447/101‑1 (MQ=255)
                            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTg                                                                            >  1:4236661/1‑101 (MQ=255)
                            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTg                                                                            <  2:3337026/101‑1 (MQ=255)
                              cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGcc                                                                          <  1:3264893/101‑1 (MQ=255)
                              cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCACCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTAATCTTGcc                                                                          >  1:673785/1‑101 (MQ=39)
                                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGCTATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGaa                                                                       >  1:1273009/1‑101 (MQ=255)
                                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGaa                                                                       <  2:2643868/101‑1 (MQ=255)
                                  ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGaaa                                                                      >  1:16496/1‑101 (MQ=255)
                                  ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGaaa                                                                      >  1:3827198/1‑101 (MQ=255)
                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCATGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAat                                                                    <  1:4425801/101‑1 (MQ=255)
                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAat                                                                    <  2:322504/101‑1 (MQ=255)
                                      gcccccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATTAA...GGGG..GGC.TTTCATCTTGCCGAAAatat                                                                  <  1:2526471/98‑1 (MQ=37)
                                      gCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAatat                                                                  <  1:4195545/101‑1 (MQ=255)
                                         cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATccc                                                               <  1:3188297/101‑1 (MQ=255)
                                             gggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCgggg                                                           >  2:1044724/1‑101 (MQ=255)
                                                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                       <  1:4613386/101‑1 (MQ=255)
                                                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                       >  2:4806118/1‑101 (MQ=255)
                                                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                       >  2:365312/1‑101 (MQ=255)
                                                    aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                   <  1:1933932/101‑1 (MQ=255)
                                                       ggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                >  2:4840296/1‑101 (MQ=255)
                                                        gggCTCTGCCCCCGTGCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAAAGTCCCGGGGGGTGAA.TGgcg                                               <  2:1259896/101‑1 (MQ=37)
                                                         ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGgcgc                                              >  1:1234036/1‑101 (MQ=255)
                                                           ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                          <  1:4295982/101‑1 (MQ=255)
                                                            tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                         <  2:2262889/96‑1 (MQ=255)
                                                            tctgcccccGTCCCGTGCCGCGACT.CTCCCGGGATATATGAAGCAAGAAGAA...GGGG..GCC.TTACATCTTGCCGAAAATATCCCGGGGCGTGAA.TGGCGCA..Gc                                         <  2:5020550/96‑1 (MQ=25)
                                                             ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..Gcc                                        >  1:3524313/5‑101 (MQ=255)
                                                                cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                     <  1:2904333/100‑1 (MQ=255)
                                                                    cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                 >  1:231295/1‑101 (MQ=255)
                                                                    cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                 >  1:2776911/1‑101 (MQ=255)
                                                                     gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                >  1:3213276/1‑101 (MQ=255)
                                                                     gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGAGAA.TGGCGCA..GCCAGAggggg                                >  2:1403680/1‑101 (MQ=255)
                                                                      tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAgggggg                               >  2:889335/1‑101 (MQ=255)
                                                                       cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                              >  1:2415653/1‑101 (MQ=255)
                                                                       cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                              <  1:4980737/101‑1 (MQ=255)
                                                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                             <  2:608220/101‑1 (MQ=255)
                                                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                             >  2:3711690/1‑101 (MQ=255)
                                                                        ccgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAAC.CGGA..TGCATTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggg                                 >  1:4872835/9‑101 (MQ=21)
                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                            <  1:3312132/101‑1 (MQ=255)
                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                            <  1:3498506/101‑1 (MQ=255)
                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                            >  1:396248/1‑101 (MQ=255)
                                                                         cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCC.TTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                >  2:2381758/8‑101 (MQ=18)
                                                                          gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                           >  1:2566735/1‑101 (MQ=255)
                                                                              gcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCC.TTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgc                           <  1:2459501/99‑1 (MQ=18)
                                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                      <  1:2591059/101‑1 (MQ=255)
                                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                      >  1:1052733/1‑101 (MQ=255)
                                                                               gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                     >  1:929201/1‑101 (MQ=255)
                                                                                  ccT.CCCCAAGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccct                  <  2:3823789/100‑3 (MQ=255)
                                                                                  aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccct                  >  1:2140057/1‑99 (MQ=255)
                                                                                  aCT.CCCCAGGGTTATTTGAGGCAAGATGTA...GGGG..GGC.TTTCATCTTGGCGAAAATTTGCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccct                  <  2:4101922/101‑3 (MQ=25)
                                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                 <  1:4041099/101‑4 (MQ=255)
                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                <  2:1645830/101‑5 (MQ=255)
                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                <  1:2181228/101‑5 (MQ=255)
                                                                                       ccccGGGATATTTGCGGCAAGGTGAA...GGGGCATGA.TTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtg                  >  1:2863925/1‑100 (MQ=2)
                                                                                       ccccGGGATATTTGCGGCAAGATGAA...GGGGCATGA.TTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtg                  >  1:3435718/1‑100 (MQ=0)
                                                                                       ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTcgc               <  2:4298079/101‑1 (MQ=255)
                                                                                           gggATATTTGCGGCAAGATGAA...GGGGCATGA.TTGCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtgcccc              <  1:4326169/101‑6 (MQ=0)
                                                                                           gggATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc           <  1:654632/101‑1 (MQ=255)
                                                                                              atatTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGggcggc        >  2:4464893/1‑101 (MQ=255)
                                                                                              atatTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGggcggc        >  1:2251467/1‑101 (MQ=255)
                                                                                               tatTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGcc       <  2:2757136/101‑1 (MQ=255)
                                                                                                  ttGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgcg    >  2:1926736/1‑101 (MQ=255)
                                                                                                   tGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGGGGGCGGCCGCGa   >  1:477324/1‑101 (MQ=37)
                                                                                                    gAGGAAAGATGAA...GGGG..GGC.TTTCATTTTGCCGAAAATTTCGGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAc  <  2:1789784/101‑1 (MQ=37)
                                                                                                     |                                                                                                            
CATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGAC  >  NC_014034/2249217‑2249414

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG