Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 243,127 A→G 100% E28E (GAA→GAG mogA → molybdopterin biosynthesis mog protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034243,1270A→G100.0% 830.9 219E28E (GAA→GAGmogAmolybdopterin biosynthesis mog protein
Reads supporting (aligned to +/- strand):  new base (100/119):  ref base (0/0):  total (100/119)

Only 100 of 230 total aligned reads displayed.

GCGCGTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAAGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTCG  >  NC_014034/243029‑243224
                                                                                                  |                                                                                                 
gcgcgTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGa                                                                                                 >  1:3646270/1‑101 (MQ=255)
  gcgTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACt                                                                                               >  1:2924704/1‑101 (MQ=255)
   cgTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTg                                                                                              >  1:4206150/1‑101 (MQ=255)
    gTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTgg                                                                                             >  1:728675/1‑101 (MQ=255)
    gTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTgg                                                                                             >  1:4792222/1‑101 (MQ=255)
       gcgaAAAAATGACGCCACGGGTTGCCATCGTCTCCGCCCCCGACCGCGCCCCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTg                                                                                          <  1:1022311/101‑1 (MQ=255)
         gcAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAg                                                                                        >  2:179042/1‑101 (MQ=255)
          cAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAGGGGCGGGCCGGGCTGCGAGGACTGGCTGAgg                                                                                       <  1:4571771/101‑1 (MQ=255)
          cAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAgg                                                                                       >  2:4231615/1‑101 (MQ=255)
           aaaaaTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGGGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAggg                                                                                      >  1:1557411/1‑101 (MQ=255)
           aaaaaTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAggg                                                                                      >  2:3869231/1‑101 (MQ=255)
            aaaaTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAgggg                                                                                     <  1:188970/101‑1 (MQ=255)
            aaaaTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGAATGAGGACAAGGGCGGGACGGGCTGCGAGGACTGGCTGAgggg                                                                                     >  2:5042002/1‑101 (MQ=255)
             aaaTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGgt                                                                                    <  1:1687786/101‑1 (MQ=255)
             aaaTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGgt                                                                                    >  2:781780/1‑101 (MQ=255)
              aaTGCCGGCACGGGTTGCCATCGTCCCCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGATGCGAGGACTGGCTGAGGGgtg                                                                                   <  1:2079924/101‑1 (MQ=255)
              aaTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGgtg                                                                                   >  2:2733849/1‑101 (MQ=255)
               aTGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGgtgg                                                                                  >  2:1717530/1‑100 (MQ=255)
                   cGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTgtcgtc                                                                              <  1:448893/101‑1 (MQ=255)
                      cACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACg                                                                           <  1:800860/101‑1 (MQ=255)
                      cACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACg                                                                           <  1:2654057/101‑1 (MQ=255)
                       aCGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGa                                                                          <  2:2924477/101‑1 (MQ=255)
                        cGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGac                                                                         <  1:1252810/101‑1 (MQ=255)
                        cGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGac                                                                         >  1:4966126/1‑101 (MQ=255)
                        cGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGac                                                                         >  1:2218038/1‑101 (MQ=255)
                          ggTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGacac                                                                       >  1:2794155/1‑101 (MQ=255)
                            ttGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACAccc                                                                     >  2:1939590/1‑101 (MQ=255)
                            ttGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACAccc                                                                     <  2:728586/101‑1 (MQ=255)
                            ttGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACAccc                                                                     <  2:3056297/101‑1 (MQ=255)
                             tGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACAcccc                                                                    <  1:3127503/101‑1 (MQ=255)
                             tGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACAcccc                                                                    >  1:3870934/1‑101 (MQ=255)
                              gCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGCGTCGTCACGACACCCCt                                                                   >  2:3303897/1‑101 (MQ=255)
                              gCCATCGTCACCGTCCCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCt                                                                   <  1:2247702/101‑1 (MQ=255)
                               ccATCGTCGCCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGACGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCtt                                                                  <  2:2057894/101‑1 (MQ=255)
                               ccATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCtt                                                                  <  1:3669505/101‑1 (MQ=255)
                                cATCGTCCCCCTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCttt                                                                 <  2:1840475/101‑1 (MQ=255)
                                cATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACGCCCCttt                                                                 <  2:2485093/101‑1 (MQ=255)
                                cATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCttt                                                                 <  2:4273074/101‑1 (MQ=255)
                                cATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCttt                                                                 <  1:4822691/101‑1 (MQ=255)
                                 ctCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTc                                                                <  1:1580651/100‑1 (MQ=255)
                                 aTCGTCTCCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTc                                                                <  1:5042332/101‑1 (MQ=255)
                                 aTCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTc                                                                <  1:3304051/101‑1 (MQ=255)
                                 aTCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTc                                                                >  2:4008647/1‑101 (MQ=255)
                                 aTCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTc                                                                <  1:1939727/101‑1 (MQ=255)
                                  tCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTcc                                                               <  1:4043997/101‑1 (MQ=255)
                                   cGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCa                                                              <  1:994691/101‑1 (MQ=255)
                                   cGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGCGCTGCGAGGACTGGCTGAGGGGGGTCGCCACGACACCCCTTTCCa                                                              >  1:3757594/1‑101 (MQ=255)
                                     tccccGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCAtt                                                            <  1:591876/98‑1 (MQ=255)
                                      cACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCAttt                                                           >  1:1120992/1‑101 (MQ=255)
                                      cACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCAttt                                                           <  2:3913054/101‑1 (MQ=255)
                                        ccGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTcc                                                         <  1:1296377/101‑1 (MQ=255)
                                          gTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCg                                                       <  2:590876/101‑1 (MQ=255)
                                            ctcCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACCCCCCTTTCCATTTCCCggg                                                     >  2:4145869/1‑101 (MQ=255)
                                              ccGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTg                                                   <  1:2955327/101‑1 (MQ=255)
                                              ccGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGGCGGCACGACACCCCTTTCCATTTCCCggggg                                                   >  2:4819831/1‑99 (MQ=255)
                                                gACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGat                                                 <  2:669276/101‑1 (MQ=255)
                                                 gccGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGatc                                                <  1:2188630/100‑1 (MQ=255)
                                                  ccGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGatca                                               <  2:3842997/101‑1 (MQ=255)
                                                  ccGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGatca                                               <  2:1156765/101‑1 (MQ=255)
                                                   cgcgCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGatcat                                              <  2:395871/101‑1 (MQ=255)
                                                   cgcgCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGatcat                                              <  2:2836218/101‑1 (MQ=255)
                                                    gcgcCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGatcatc                                             <  2:2120914/101‑1 (MQ=255)
                                                     cgcCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATcc                                            <  2:1738134/101‑1 (MQ=255)
                                                     cgcCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATcc                                            >  1:28647/1‑101 (MQ=255)
                                                       ccTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATcccc                                          >  2:1551806/1‑101 (MQ=255)
                                                       ccTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATcccc                                          <  2:3627153/101‑1 (MQ=255)
                                                        cTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCg                                         <  2:4965796/101‑1 (MQ=255)
                                                          cgcgcgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGAc                                       >  2:129068/1‑101 (MQ=255)
                                                          cgcgcgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGAc                                       >  1:2310721/1‑101 (MQ=255)
                                                          cgcgcgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGAc                                       <  1:4577925/101‑1 (MQ=255)
                                                           gcgcgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCCCGACAACCCTTTCCATTTCCCGGGGGATCATCCCCGACg                                      >  1:4375557/1‑101 (MQ=255)
                                                           gcgcgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACg                                      <  2:4176986/101‑1 (MQ=255)
                                                            cgcgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACgg                                     >  1:1719367/1‑101 (MQ=255)
                                                              cgGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGcc                                   >  1:639282/1‑101 (MQ=255)
                                                               ggggAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGGCTGGCTGAGGGGTGTCGTCACGACAGCCCTTTCCCTTTCCCGGGTGATCATCCCCGACGGCcg                                  >  2:475988/1‑101 (MQ=255)
                                                               ggggAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCcg                                  >  2:82135/1‑101 (MQ=255)
                                                               ggggAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGGCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCcg                                  >  2:3122609/1‑101 (MQ=255)
                                                                 ggAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCcgcg                                >  1:590312/1‑101 (MQ=255)
                                                                 ggAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGGGTCGTCACGACACCCCTTTCCATTTCCCGTGTGATCATCCCCGACGGCcgcg                                >  2:4590555/1‑101 (MQ=255)
                                                                  gAGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCcgcgt                               <  1:4613226/101‑2 (MQ=255)
                                                                   aGTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGAc                              <  2:2240027/101‑1 (MQ=255)
                                                                    gTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACa                             >  1:4672085/1‑101 (MQ=255)
                                                                    gTATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACa                             >  2:2971750/1‑101 (MQ=255)
                                                                     tATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAg                            >  1:3031092/1‑101 (MQ=255)
                                                                     tATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAg                            >  1:429704/1‑101 (MQ=255)
                                                                     tATGAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAg                            <  1:704395/101‑1 (MQ=255)
                                                                        gAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGt                         >  2:1054115/1‑101 (MQ=255)
                                                                        gAGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGt                         >  2:530241/1‑101 (MQ=255)
                                                                         aGGACAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTc                        <  1:1079198/101‑1 (MQ=255)
                                                                            aCAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGcc                     <  1:1204373/101‑1 (MQ=255)
                                                                             cAAGGGCGGGCCGGGCTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCg                    <  2:1369711/101‑1 (MQ=255)
                                                                                   cgggccgggcTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGActgcc              >  1:3609182/1‑101 (MQ=255)
                                                                                          ggcTGCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCgg       >  2:3264455/1‑101 (MQ=255)
                                                                                              gCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTc   <  2:3534931/101‑1 (MQ=255)
                                                                                              gCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTc   >  1:3214182/1‑101 (MQ=255)
                                                                                              gCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTc   <  1:3097855/101‑1 (MQ=255)
                                                                                              gCGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTc   >  1:1841851/1‑101 (MQ=255)
                                                                                               cGAGGACTGGCTGAGGGGTGTCGTCACGACCCCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTCg  <  2:2590765/101‑1 (MQ=255)
                                                                                               cGAGGACTGGCTGAGGGGTGTCGTCACGACCCCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTCg  <  1:2228949/101‑1 (MQ=255)
                                                                                               cGAGGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTCg  >  1:3469142/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGCGTGGCGCAAAAATGACGGCACGGGTTGCCATCGTCACCGTCTCCGACCGCGCCTCGCGCGGGGAGTATGAGGACAAGGGCGGGCCGGGCTGCGAAGACTGGCTGAGGGGTGTCGTCACGACACCCCTTTCCATTTCCCGGGTGATCATCCCCGACGGCCGCGACAGCGTCGCCGACTGCCTGCGCGGCTTCG  >  NC_014034/243029‑243224

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG