Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,048 G→A 3.2% intergenic (+96/‑146) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0480G→A 3.2% 5.7 ‑0.4 ‑1.1157intergenic (+96/‑146)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (87/63):  total (93/64)
Fisher's exact test strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.30e-02

Only 100 of 176 total aligned reads displayed.

AGTAAGGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCA  >  NC_014034/2493948‑2494147
                                                                                                    |                                                                                                           
gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                                             >  1:551448/6‑101 (MQ=255)
gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                                             >  2:50858/6‑101 (MQ=255)
 taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCCAGATGAAACCGGGTGCCTTTCATCTTGCCCGAAATATTCCggg                                                                                                            >  2:1893391/5‑101 (MQ=18)
 taaggagggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCCAGATGAAACCGGGTGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                            >  1:333415/7‑101 (MQ=17)
  aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                                           >  1:3722445/4‑101 (MQ=255)
  aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                                           <  1:2046447/98‑1 (MQ=255)
   aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                                                          >  1:4942366/3‑101 (MQ=255)
   aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                                                          >  2:4782252/3‑101 (MQ=255)
       gggggCGCTGCCCCCCGTGCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATCAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                      <  1:5026068/101‑1 (MQ=18)
       gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                      <  2:560147/101‑1 (MQ=255)
        ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATTCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                                                     <  2:1670615/101‑1 (MQ=255)
         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                   <  1:231201/101‑1 (MQ=255)
         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                   <  1:2724890/101‑1 (MQ=255)
         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGCTATTTGCAGCAAGATGAAACCGGAAGCATTTCCTCTTTCCCGAAAAATCCCggggggggcat                                                                                                    >  1:1162576/1‑96 (MQ=17)
          ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                  <  1:2366847/101‑1 (MQ=255)
             gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                             >  2:2348482/1‑101 (MQ=255)
             ccTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                             <  1:1388401/100‑1 (MQ=255)
                ccccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                         <  2:295632/100‑3 (MQ=255)
                 cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                       <  1:2236576/101‑5 (MQ=255)
                 cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                       <  2:1958789/101‑5 (MQ=255)
                 cccccCGTCCGCAAGCGGCCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                                                        <  2:3354399/101‑4 (MQ=21)
                 cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                                                        <  1:779015/101‑4 (MQ=255)
                    cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAAAATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                     >  1:2081755/5‑95 (MQ=21)
                       gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                  <  2:4234184/100‑10 (MQ=255)
                       gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                  >  2:4627169/2‑92 (MQ=255)
                        tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                                                                 <  1:1113887/101‑11 (MQ=255)
                             aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCa                                                                            >  2:2286492/1‑101 (MQ=18)
                               gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagc                                                                          <  2:2245006/101‑3 (MQ=18)
                                cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGATATATCCCGGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                         >  2:5013465/1‑98 (MQ=17)
                                cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                         >  2:234936/1‑98 (MQ=18)
                                 ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGTGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                                                        >  1:4296424/1‑97 (MQ=17)
                                 ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                                                        >  1:4922635/1‑97 (MQ=17)
                                 ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                                                        >  1:4959002/1‑97 (MQ=17)
                                  gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c                                                                    >  1:1658484/1‑101 (MQ=17)
                                  gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c                                                                    >  1:2912258/1‑101 (MQ=18)
                                   aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c.c                                                                  >  2:380330/1‑101 (MQ=18)
                                    cTCCCCCCGGGATATTTGCAGCAAGTTGAAACCGGATGCCCTTCATCTTGCCCGAAATATCCCGGGGGGTGAG.TCGC..GAAG.CCACAGGGGGGC.AGCG.c.c.cc                                                                 >  2:1792817/1‑101 (MQ=17)
                                     tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c.ccc                                                                >  2:3924517/1‑101 (MQ=17)
                                       cccccGGAATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCGGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c.cccct                                                              <  2:4240711/101‑3 (MQ=17)
                                           cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCCTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGccc                                                           >  1:1996975/1‑101 (MQ=17)
                                            gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGcccc                                                          >  2:3722405/1‑101 (MQ=17)
                                             ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.C.CCCTGCCCcg                                                         >  1:414303/1‑101 (MQ=2)
                                             ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcg                                                         <  1:1580824/101‑1 (MQ=17)
                                             ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcg                                                         >  1:4557965/1‑101 (MQ=17)
                                              gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.C.CCCTGCCCcgc                                                        >  1:1106325/1‑100 (MQ=2)
                                              gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GCCAGAGGGGGCCAGCGCC.C.CCCTGCCCcgg                                                        >  1:1500262/1‑101 (MQ=18)
                                               atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.C.CCCTGCCCcgcc                                                       <  2:2567939/101‑3 (MQ=2)
                                               atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcggc                                                       >  1:1947906/1‑101 (MQ=17)
                                                tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcggcg                                                      >  1:2956470/1‑101 (MQ=17)
                                                tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcggcg                                                      >  2:4221845/1‑101 (MQ=18)
                                                   ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTc                                                   >  1:3754824/1‑101 (MQ=18)
                                                    tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTcc                                                  >  1:3887436/1‑101 (MQ=17)
                                                    tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTcc                                                  >  1:3037526/1‑101 (MQ=17)
                                                    tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGG.GAGCG.C.CGCCCTGCCCCGGCGGTcc                                                  >  2:1902392/1‑101 (MQ=18)
                                                    tGCACNNAGATGCAACCGGATTCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTcc                                                  <  1:1976333/101‑1 (MQ=14)
                                                      cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcggcggggcgt                                                >  2:4463382/1‑96 (MQ=17)
                                                        gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTgg                                              >  1:4272173/1‑101 (MQ=17)
                                                           aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCCGCGGTCCGTggcgg                                           >  2:3144664/1‑101 (MQ=17)
                                                            gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTggcggc                                          >  1:991197/1‑101 (MQ=17)
                                                                aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                      >  2:29184/1‑101 (MQ=17)
                                                                 aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                     <  2:2912032/101‑1 (MQ=17)
                                                                  aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                    >  2:3661741/1‑101 (MQ=18)
                                                                  aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                    <  2:3904594/101‑1 (MQ=17)
                                                                   ccGGATGCCTTTCATCTTCCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                   <  2:1609947/101‑1 (MQ=17)
                                                                     ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                 <  2:3017367/101‑1 (MQ=17)
                                                                      gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCA.CAC.CCCTGCCCCGGCGGTCCGTGGCGGGGCCCTGAcgg                                >  1:836473/1‑101 (MQ=12)
                                                                        tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                              <  1:740217/101‑1 (MQ=17)
                                                                        tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGT.GCGGCGCCTTGAcgggcg                             >  2:218413/1‑101 (MQ=17)
                                                                           cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                           >  2:416665/1‑101 (MQ=18)
                                                                            tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                          >  2:1200603/1‑101 (MQ=18)
                                                                             ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                         >  2:3039833/1‑101 (MQ=18)
                                                                             ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                         >  2:76190/1‑101 (MQ=18)
                                                                             ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGG.AAGCGCC.C.CCCTGCCCCGGCGGGCCGCGGCGGCGCCTTGCGGGGCggggg                         >  2:1531078/1‑101 (MQ=9)
                                                                             ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.CGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                         >  2:1903303/1‑101 (MQ=18)
                                                                              tCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTCCCCCGGCGGTCCGTGGCGGCGCCTTGACCGGGgggggg                        >  2:1239428/1‑100 (MQ=9)
                                                                                aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                      >  2:3072130/1‑101 (MQ=18)
                                                                                 tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                     >  2:364132/1‑101 (MQ=18)
                                                                                  cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                    <  2:4405102/101‑1 (MQ=18)
                                                                                    tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGGCTTGACGGGCGGGGGCGCGGGc                  >  2:4999236/1‑101 (MQ=18)
                                                                                    tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                  <  1:3325569/101‑1 (MQ=18)
                                                                                    tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                  <  1:380383/101‑1 (MQ=18)
                                                                                     gCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GCCAGAGGGGGCCAGCGCC.C.CCCTCCCCCGGCGGTCCGTGGCGGCGCCTTCACCTGGGGGGGCGCggggc                 >  2:621723/1‑99 (MQ=9)
                                                                                      gccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                >  2:2132524/2‑101 (MQ=18)
                                                                                      cccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                <  1:2606143/101‑1 (MQ=18)
                                                                                       ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt               <  1:2861406/101‑1 (MQ=18)
                                                                                       ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt               <  1:2961129/101‑1 (MQ=18)
                                                                                       ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt               >  2:2835915/1‑101 (MQ=18)
                                                                                         gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa             <  1:2443983/101‑1 (MQ=18)
                                                                                          aaaTATCCCGTGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAAc            >  1:3450778/1‑101 (MQ=18)
                                                                                          aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGGCGGGCGGGGGCGCGGGCATTAAc            <  2:1701948/101‑1 (MQ=18)
                                                                                          aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc            >  1:3127500/1‑101 (MQ=18)
                                                                                          aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc            >  2:2218909/1‑101 (MQ=18)
                                                                                          aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCCCGGGGATTAAc            >  2:1249508/1‑101 (MQ=18)
                                                                                            atatCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg          >  2:4307076/1‑101 (MQ=21)
                                                                                             tatCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa         >  1:4144164/1‑101 (MQ=21)
                                                                                              atCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGG.AGCCC.C.C.CCCTGCCCCGGCGGGCCGGTGGGGCGCCTTGGCCGGGGGGGGGGGGGGGATTTACCCaaa       >  1:3547767/1‑101 (MQ=2)
                                                                                              agcccGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa        <  1:305762/99‑1 (MQ=21)
                                                                                               tCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa       <  1:4718866/101‑1 (MQ=21)
                                                                                                   ggggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc   <  1:4782575/101‑1 (MQ=25)
                                                                                                    gggggTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa  >  2:3177538/1‑101 (MQ=25)
                                                                                                    |                                                                                                           
AGTAAGGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCA  >  NC_014034/2493948‑2494147

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG