Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,531,575 G→A 100% L542L (CTC→CTT RCAP_rcc02362 ← competence protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,531,5750G→A100.0% 560.8 144L542L (CTC→CTTRCAP_rcc02362competence protein
Reads supporting (aligned to +/- strand):  new base (81/63):  ref base (0/0):  total (81/63)

Only 100 of 161 total aligned reads displayed.

AGCGCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACGAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCG  >  NC_014034/2531477‑2531672
                                                                                                  |                                                                                                 
aGCGCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaag                                                                                                 >  1:1079950/1‑101 (MQ=255)
aGCGCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaag                                                                                                 <  1:2629850/101‑1 (MQ=255)
aGCGCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaag                                                                                                 <  1:579939/101‑1 (MQ=255)
   gcccgcccCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaagcgc                                                                                              <  1:4720351/101‑1 (MQ=255)
   gcccgcccCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaagcgc                                                                                              <  1:592185/101‑1 (MQ=255)
    cccgcccCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCGGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaagcgcg                                                                                             <  2:4372371/101‑1 (MQ=255)
    cccgcccCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCAcaagcgcg                                                                                             >  1:452471/1‑101 (MQ=255)
          ccTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGcc                                                                                       <  1:2548368/101‑1 (MQ=255)
           cTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCa                                                                                      <  1:3871981/101‑1 (MQ=255)
           cTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCa                                                                                      >  2:1822023/1‑101 (MQ=255)
             cGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGc                                                                                    <  1:1046134/101‑1 (MQ=255)
              gggCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCa                                                                                   >  2:1674990/1‑101 (MQ=255)
               ggCGTCATCAGCCGGACAAGCGCGCGCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCAt                                                                                  <  2:1138039/101‑1 (MQ=255)
                gCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATc                                                                                 <  1:2772893/101‑1 (MQ=255)
                gCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGAGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATc                                                                                 <  1:2711961/101‑1 (MQ=255)
                 cGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcg                                                                                <  2:3242833/101‑1 (MQ=255)
                 cGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcg                                                                                <  2:73004/101‑1 (MQ=255)
                 cGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcc                                                                                >  1:1717464/1‑100 (MQ=255)
                  gTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgc                                                                               <  2:4480792/101‑1 (MQ=255)
                  gTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgc                                                                               <  1:1298512/101‑1 (MQ=255)
                   tcagcaGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAACCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATcgcc                                                                              <  1:1561926/101‑1 (MQ=255)
                      tcaGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCcgcg                                                                           <  1:3484931/101‑1 (MQ=255)
                       caGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCcgcgc                                                                          >  2:3434474/1‑101 (MQ=255)
                        aGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCcgcgcg                                                                         >  2:3204545/1‑101 (MQ=255)
                        aGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCcgcgcg                                                                         >  1:1841306/1‑101 (MQ=255)
                           ccGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATGGCCGCGCGGaa                                                                      >  2:1574402/1‑101 (MQ=255)
                           ccGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGaa                                                                      >  1:2895532/1‑101 (MQ=255)
                           ccGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGaa                                                                      <  1:2254437/101‑1 (MQ=255)
                            cGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAc                                                                     <  1:952215/101‑1 (MQ=255)
                             gACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAcg                                                                    <  1:1689962/101‑1 (MQ=255)
                             gACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAcg                                                                    >  2:1975627/1‑101 (MQ=255)
                              aCAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAcgc                                                                   <  2:3869705/101‑1 (MQ=255)
                               cAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCCAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAcgcg                                                                  <  2:2573927/101‑1 (MQ=255)
                               cAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAcgcg                                                                  >  1:13518/1‑101 (MQ=255)
                               cAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAAcgcg                                                                  >  1:435717/1‑101 (MQ=255)
                                aaGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACgcgc                                                                 >  2:4427892/1‑101 (MQ=255)
                                aaGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACgcgc                                                                 >  1:71094/1‑101 (MQ=255)
                                aaGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACgcgc                                                                 <  2:5003572/101‑1 (MQ=255)
                                  gcgcgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGccc                                                               <  1:740689/101‑1 (MQ=255)
                                   cgcgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCTGCGCGGAACGCGcccc                                                              >  1:3121101/1‑101 (MQ=255)
                                   cgcgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGcccc                                                              <  2:795009/101‑1 (MQ=255)
                                   cgcgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGcccc                                                              <  2:2895319/101‑1 (MQ=255)
                                   cgcgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGcccc                                                              <  2:2054202/101‑1 (MQ=255)
                                    gcgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCcg                                                             <  1:299091/101‑1 (MQ=255)
                                     cgcCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCcgc                                                            >  1:3168079/1‑101 (MQ=255)
                                       cccccGGGCGCGATCGGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGa                                                          <  1:4024693/101‑1 (MQ=255)
                                        ccccGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGAc                                                         <  2:1851581/101‑1 (MQ=255)
                                          ccGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAg                                                       >  2:3826976/1‑101 (MQ=255)
                                          ccGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAg                                                       <  2:2821634/101‑1 (MQ=255)
                                           cGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGc                                                      >  2:585559/1‑101 (MQ=255)
                                           cGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGc                                                      <  2:4237927/101‑1 (MQ=255)
                                           cGGGCGCGATCAGCAGCGCCGGACGGGCGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGc                                                      <  2:1421305/101‑1 (MQ=255)
                                            ggtcgcgATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCa                                                     <  2:3038473/98‑1 (MQ=255)
                                            gggCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCa                                                     >  1:2167579/1‑101 (MQ=255)
                                             ggCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAc                                                    >  1:2019692/1‑101 (MQ=255)
                                              gcgcGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAcc                                                   <  2:4017710/101‑1 (MQ=255)
                                               cgcgATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccg                                                  <  1:1666597/101‑1 (MQ=255)
                                                gcgATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgc                                                 >  2:2052239/1‑101 (MQ=255)
                                                 cgATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgcc                                                >  2:2014361/1‑101 (MQ=255)
                                                 cgATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgcc                                                <  1:660443/101‑1 (MQ=255)
                                                 cgATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgcc                                                <  1:3314357/101‑1 (MQ=255)
                                                  gATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgccg                                               >  1:1979295/1‑101 (MQ=255)
                                                  gATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCAccgccg                                               >  1:3604583/1‑101 (MQ=255)
                                                         agcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGc                                        >  1:4981029/1‑101 (MQ=255)
                                                         agcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGc                                        >  1:2276944/1‑101 (MQ=255)
                                                         agcGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGc                                        >  2:3011931/1‑101 (MQ=255)
                                                         agcGCCGGACGGGGGGCAAGCACCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGc                                        >  1:2876017/1‑101 (MQ=255)
                                                          gcgcGGGACGGGGGCCAAGCCCCAAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGcc                                       <  2:4966090/101‑1 (MQ=255)
                                                          gccccGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGcc                                       <  2:980482/98‑1 (MQ=255)
                                                             ccGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCa                                    >  1:1804007/1‑101 (MQ=255)
                                                               ggACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCGAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAg                                  >  2:4368840/1‑101 (MQ=255)
                                                               ggACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAg                                  >  1:3340932/1‑101 (MQ=255)
                                                               ggACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAg                                  >  1:3847254/1‑101 (MQ=255)
                                                               ggACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAg                                  >  2:3790485/1‑101 (MQ=255)
                                                               ggACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAg                                  >  1:2191070/1‑101 (MQ=255)
                                                                 aCGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAgcg                                >  1:3956040/1‑101 (MQ=255)
                                                                  cGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAgcgc                               <  1:2605708/101‑1 (MQ=255)
                                                                  cGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAgcgc                               >  1:1574051/1‑101 (MQ=255)
                                                                    gggggCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCa                             >  2:3669528/1‑101 (MQ=255)
                                                                     ggggCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAg                            >  1:4278248/1‑101 (MQ=255)
                                                                     ggggCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAg                            <  2:2465687/101‑1 (MQ=255)
                                                                      gggCAAGCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGc                           <  2:4188641/101‑1 (MQ=255)
                                                                      gggCAACCCCCCAGCCCGCAAGGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGc                           <  2:3749579/101‑1 (MQ=255)
                                                                         cagacccccAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCAcc                        <  1:4507531/97‑1 (MQ=255)
                                                                            gCCCCCAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGgc                     <  1:4559671/101‑1 (MQ=255)
                                                                              ccccAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGgcag                   >  2:4489352/1‑101 (MQ=255)
                                                                               cccAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGgcagc                  <  2:13510/101‑1 (MQ=255)
                                                                               cccAGCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGgcagc                  <  1:3375898/101‑1 (MQ=255)
                                                                                   gCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCAccc              <  2:2499362/101‑1 (MQ=255)
                                                                                   gCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCAccc              <  1:1975771/101‑1 (MQ=255)
                                                                                   gCCCGCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCAccc              <  2:1549493/101‑1 (MQ=255)
                                                                                       gCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGcc          <  1:2161015/101‑1 (MQ=255)
                                                                                       gCAAAGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGcc          >  2:1770033/1‑101 (MQ=255)
                                                                                         aaaGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCCGCCgg        >  2:1498575/1‑101 (MQ=255)
                                                                                         aaaGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCgg        >  2:4085559/1‑101 (MQ=255)
                                                                                         aaaGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCgg        >  1:4570619/1‑101 (MQ=255)
                                                                                         aaaGGCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCgg        >  1:1374233/1‑101 (MQ=255)
                                                                                            ggCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGggcg     >  2:2170623/1‑101 (MQ=255)
                                                                                            ggCCACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGggcg     >  2:3712298/1‑101 (MQ=255)
                                                                                               cACAAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGgcggcg  >  1:4945468/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
AGCGCCCGCCCCTCGGGCGTCATCAGCCCGACAAGCGCGCCCCCGGGCGCGATCAGCAGCGCCGGACGGGGGGCAAGCCCCCAGCCCGCAAAGGCCACGAGCGCGAGCGCCAGCATCGCCGCGCGGAACGCGCCCCGCGACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCG  >  NC_014034/2531477‑2531672

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG