BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,566,555	C→T	100%	intergenic (+27/+167)	purD → / ← sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,566,555	0	C→T	100.0%	579.4	202	intergenic (+27/+167)	purD/sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand): new base (35/166): ref base (0/0): total (35/167)

Only 100 of 225 total aligned reads displayed.


TATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.GG.GGG.CGCTGCCCCC.CTC.CT..G..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCC  >  NC_014034/2566457‑2566644


tATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACGAAAAG.GG.GGG.CGGTGCCCCc.ccc.ct                                                                                              >  2:967782/1‑101 (MQ=255)


 aTGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..g                                                                                           <  2:1108243/101‑1 (MQ=255)


 aTGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..g                                                                                           >  2:3418250/1‑101 (MQ=255)


    cGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..ggcc                                                                                        <  1:146102/101‑4 (MQ=39)


     gATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGGGCGCGCCCTGGGACGAAAAC.GG.GGG.GGCTGCCCCC.CCC.CT..ggccc                                                                                       >  2:2812003/1‑97 (MQ=255)


      ggggTTGATGCGATCGACTGGCCCGGGGTGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.GGAGGG.CGCTGCCCCC.CTC.TT..gttctt                                                                                      <  2:162798/99‑6 (MQ=255)


       tGGTCGAGCCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..ggccttc                                                                                     <  2:4084918/101‑7 (MQ=37)


          tCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..ggccttcggc                                                                                  <  2:744489/101‑10 (MQ=37)


                cGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTc                                                                            <  1:1152670/101‑1 (MQ=37)


                cGAGCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTc                                                                            <  1:1697672/101‑1 (MQ=25)


                 gATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCa                                                                           <  2:1860831/101‑1 (MQ=37)


                   tCGACTGGCCCGGGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCAcc                                                                         <  1:2288985/101‑1 (MQ=25)


                     gACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGGTGCCCCC.CTC.CTTGG..CCTCGGCCCATTTAcccc                                                                       >  2:195032/1‑101 (MQ=255)


                       cTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCAcccccc                                                                     <  1:3407201/101‑1 (MQ=25)


                       cTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCAcccccc                                                                     <  1:2895076/101‑1 (MQ=25)


                        tGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCg                                                                    <  2:2580723/101‑1 (MQ=25)


                        tGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CCC.CT..GGCCCTCCGCCGATTCACCCCCCg                                                                    >  1:3059487/1‑101 (MQ=255)


                           cccGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGa                                                                 <  2:3895147/101‑1 (MQ=25)


                             cGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGGTGCCCCC.CTC.TT..GGCCTTCGGCCCATTCACCCCCCGGGaca                                                               >  2:554286/1‑99 (MQ=25)


                             cGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGata                                                               <  1:3046968/101‑1 (MQ=25)


                              gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGatat                                                              <  1:3757308/101‑1 (MQ=25)


                               aGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATAtt                                                             <  1:5046351/101‑1 (MQ=21)


                                ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATAttt                                                            <  1:4823843/101‑1 (MQ=21)


                                ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATAttt                                                            <  2:82969/101‑1 (MQ=21)


                                 gggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTc                                                           <  2:3172939/101‑1 (MQ=21)


                                  ggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCTCCCGGGATATTTCg                                                          <  2:4988316/101‑1 (MQ=21)


                                  ggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CCC.TT..GGCCTTCGGCCCATTCACCCCCCCGGAGATTTct                                                          >  2:201523/1‑100 (MQ=21)


                                   gTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.GGCTGCCCCC.CTC.TT..GGCCTTCGGCCCATTTCCCCCCCGGGGGATTTCgg                                                         >  1:2398914/1‑101 (MQ=21)


                                    ttCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCggg                                                        >  2:2644884/1‑101 (MQ=21)


                                     tCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGc                                                       <  2:2196847/101‑1 (MQ=21)


                                      cTGCCGCCGCGACTTCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCa                                                      <  2:213462/101‑1 (MQ=21)


                                        gccgccGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAg                                                    <  1:1811994/101‑1 (MQ=21)


                                        gccgccGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAg                                                    <  2:404170/101‑1 (MQ=21)


                                        gccgccGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAg                                                    <  1:2592847/101‑1 (MQ=21)


                                         ccgccgCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGa                                                   <  1:4252492/101‑1 (MQ=21)


                                          cgccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAt                                                  <  2:475921/101‑1 (MQ=21)


                                          cgccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAt                                                  <  1:3134645/101‑1 (MQ=21)


                                          cgccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAt                                                  <  1:4733697/101‑1 (MQ=21)


                                           gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                 <  1:758907/101‑1 (MQ=21)


                                            ccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaa                                                <  2:1821522/101‑1 (MQ=21)


                                            ccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaa                                                <  2:4311421/101‑1 (MQ=21)


                                             cgcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaaa                                               <  1:431612/101‑1 (MQ=21)


                                             cgcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaaa                                               <  1:4025861/101‑1 (MQ=21)


                                              gtgACTTCGGCGGGCGCGCGCTCTGAGACA.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAaag                                              <  1:2206603/99‑1 (MQ=18)


                                               cgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaaga                                             <  2:2991831/101‑1 (MQ=21)


                                                gACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCAACCCCCGGGATATTTCGGGCAAGATAaagaa                                            <  1:1375187/101‑1 (MQ=21)


                                                 aCATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTTGGGCAAGATAaaaaag                                           >  1:1738075/1‑101 (MQ=18)


                                                 aCATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTACGGGCAAGATAaagaag                                           <  2:2525570/101‑1 (MQ=21)


                                                  cATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAgg                                          <  1:48548/101‑1 (MQ=21)


                                                  cATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAgg                                          <  2:3278843/101‑1 (MQ=21)


                                                  cATCGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAgg                                          <  2:610399/101‑1 (MQ=21)


                                                     cGGCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCt                                       <  1:535576/101‑1 (MQ=21)


                                                      ggCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                      <  2:3402325/101‑1 (MQ=21)


                                                      ggCTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                      >  2:4666595/1‑101 (MQ=21)


                                                        cTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                    <  1:1041996/101‑1 (MQ=21)


                                                        cTGGCGCGCGCTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                    <  2:174030/101‑1 (MQ=21)


                                                          ggCGCGCGCTCTGAAACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                  <  1:3651329/101‑1 (MQ=21)


                                                            cgcgcgcTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                <  1:2093234/101‑1 (MQ=21)


                                                            cgcgcgcTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                <  1:226240/101‑1 (MQ=21)


                                                             gcgcgcTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                               <  2:4016807/101‑1 (MQ=21)


                                                             gcgcgcTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                               <  1:4426888/101‑1 (MQ=21)


                                                              cgcgcTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                              <  2:192013/101‑1 (MQ=21)


                                                              cgcgcTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                              <  2:3302022/101‑1 (MQ=21)


                                                              cgcgcTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                              <  2:2870611/101‑1 (MQ=21)


                                                                cgcTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                            <  1:3208980/101‑1 (MQ=21)


                                                                cgcTCTGAGACG.AAAC.GG.GGGTCGCTTCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                            <  1:1616734/101‑1 (MQ=25)


                                                                 gcTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                           <  1:2565977/101‑1 (MQ=21)


                                                                 gcTCTGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                           <  1:3191357/101‑1 (MQ=21)


                                                                  ctctGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                          <  2:3411039/101‑1 (MQ=21)


                                                                  ctctGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGAAATTTCGGGGCAGAAAAAAAAGGGCTTTTTCttttcttt                          >  1:899201/1‑96 (MQ=9)


                                                                   tctGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                         >  2:4397614/1‑101 (MQ=21)


                                                                   tctGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTCTCCTTTCATCtt                         <  1:2728105/101‑1 (MQ=21)


                                                                    ctGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                        <  2:4898629/101‑1 (MQ=21)


                                                                    ctGAGACG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                        <  1:582768/101‑1 (MQ=21)


                                                                      gagaCG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGcc                      >  1:4110983/1‑101 (MQ=21)


                                                                       agaCG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                     <  1:191171/101‑1 (MQ=21)


                                                                         aCG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGa                   <  2:539174/101‑1 (MQ=21)


                                                                         aCG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGa                   <  2:4727329/101‑1 (MQ=21)


                                                                          cG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaa                  >  1:3357414/1‑101 (MQ=21)


                                                                          cG.AAACGGG.GGG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGACATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaa                  >  2:613597/1‑101 (MQ=21)


                                                                             aaacggg.ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                <  1:3944356/96‑1 (MQ=21)


                                                                             aaacggg.ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                >  1:3827214/6‑101 (MQ=21)


                                                                             aaacggg.ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                <  2:107585/96‑1 (MQ=21)


                                                                             aaacggg.ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCCGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCCAAat                >  1:2033105/6‑101 (MQ=21)


                                                                              ggcagg.ggg.CGCTG.CCCC.CTC.TT..GGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgg         >  1:4977195/5‑101 (MQ=0)


                                                                               acggg.ggg.CGCTTCCCCC.CTCTTT..GC.CTTCGGCCAATTCACCCCCCGGGATTTTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat              <  1:853625/98‑1 (MQ=25)


                                                                               acggg.ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCTCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat              <  2:3198269/98‑1 (MQ=25)


                                                                               acggg.ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat              <  1:2975659/98‑1 (MQ=25)


                                                                               acggg.ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat              <  1:2129400/98‑1 (MQ=25)


                                                                                cggg.ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATc             <  2:237318/99‑1 (MQ=25)


                                                                                cggg.ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATc             <  2:1023372/99‑1 (MQ=25)


                                                                                cggg.ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATc             >  2:3038455/3‑101 (MQ=25)


                                                                                cggg.ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTACTTTCATCTTGCCCGAAATATc             >  2:1100723/3‑101 (MQ=21)


                                                                                  gg.ggg.CGCTGCCCCCTCTT.GG..C..CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggggg     <  2:4231871/101‑2 (MQ=0)


                                                                                  gg.ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc           <  2:2453882/101‑1 (MQ=25)


                                                                                  gg.ggg.CGCTG.CCCC.CTC.TT..GGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggggg     >  2:1480212/1‑100 (MQ=0)


                                                                                   g.ggg.CCCTGCCCCC.TTT.TG..GC.CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggggga    <  2:359618/101‑3 (MQ=2)


                                                                                     ggg.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCgg         <  2:2353969/101‑1 (MQ=37)


                                                                                         cgcTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCggggg      <  2:4869814/101‑1 (MQ=37)


                                                                                             gCCCCC.CTC.TT..GGCCTTCGGCCAATTCTCCCCCCGGGATATTTCGGTCAAGATAAAGAAGGTCTTTTCCTTTCATCTTTCCCGAAATATCCCGGGGGAGcc  <  1:709533/101‑1 (MQ=25)


TATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.GG.GGG.CGCTGCCCCC.CTC.CT..G..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCC  >  NC_014034/2566457‑2566644

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG