Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,609 T→G 2.5% intergenic (+81/+113) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,6090T→G 2.5% 4.6 ‑0.4 ‑0.0205intergenic (+81/+113)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (100/98):  total (102/103)
Fisher's exact test strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 228 total aligned reads displayed.

CTGGCGCGCGCTCTGAGACGAAAC.GGGGG.CGCTGCCCCC.CTC.CTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAG  >  NC_014034/2566513‑2566709
                                                                                                      |                                                                                                     
cTGGCGCGCGCTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                                                                                      <  2:174030/101‑1 (MQ=21)
 tGGCGCGCGCTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                                                                                     <  2:3994153/101‑1 (MQ=21)
   gcgcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCt                                                                                                   >  1:4505293/1‑101 (MQ=21)
    cgcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                  <  1:226240/101‑1 (MQ=21)
      cgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                                <  2:2870611/101‑1 (MQ=21)
      cgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                                <  2:1918172/101‑1 (MQ=21)
       gcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCa                                                                                               <  1:2952744/101‑1 (MQ=21)
        cgcTCTGAGACGAAAC.GGGGGTCGCTTCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                                                                              <  1:1616734/101‑1 (MQ=25)
         gcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                             <  1:3191357/101‑1 (MQ=21)
          ctctGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTTATCt                                                                                            >  1:2119601/1‑101 (MQ=21)
          ctctGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                                                                                            <  2:3411039/101‑1 (MQ=21)
          ctctGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                                                                                            >  2:4678419/1‑101 (MQ=21)
          ctctGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                                                                                            >  2:2387314/1‑101 (MQ=21)
           tctGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                                                                                           <  1:88760/101‑1 (MQ=21)
            ctGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                                                                          <  1:582768/101‑1 (MQ=21)
            ctGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                                                                          <  2:4898629/101‑1 (MQ=21)
            ctGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTATCCTTTCATCTTg                                                                                          <  2:1974418/101‑1 (MQ=21)
             tGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGc                                                                                         <  1:4249700/101‑1 (MQ=21)
             tGAGACGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGAAATCTCGGGCAAGATAAAGAAGGTCGTTTCCTTTCATCTTGc                                                                                         >  1:4849067/1‑101 (MQ=21)
               agaCGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                                                                                       <  1:1748694/101‑1 (MQ=21)
                 aCGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.a                                                                                    <  2:539174/101‑1 (MQ=21)
                  cGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.aa                                                                                   >  1:3357414/1‑101 (MQ=21)
                  cGAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGACATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.aa                                                                                   >  2:613597/1‑101 (MQ=21)
                   gAAACGGGGGG.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.aaa                                                                                  >  2:229319/1‑101 (MQ=21)
                    aaacgggggg.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAat                                                                                 <  2:107585/96‑1 (MQ=21)
                    aaacgggggg.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAat                                                                                 <  1:3944356/96‑1 (MQ=21)
                    aaacgggggg.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAat                                                                                 >  1:3827214/6‑101 (MQ=21)
                    aaacgggggg.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGAAATTCCGGCCAAAAAAAGAAAGGACTTTTCTTTTCTTCTTCCCCG.aaaa                                                                                 >  2:4359411/6‑100 (MQ=2)
                    aaacgggggg.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCCGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCC.AAat                                                                                 >  1:2033105/6‑101 (MQ=21)
                     ggcaggggg.CGCTG.CCCC.CTC.TTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGC.AAATATCCCgg                                                                          >  1:4977195/5‑101 (MQ=0)
                      acgggggg.CGCTTCCCCC.CTCTTTGC.CTTCGGCCAATTCACCCCCCGGGATTTTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAatat                                                                               <  1:853625/98‑1 (MQ=25)
                      acgggggg.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAatat                                                                               <  1:2975659/98‑1 (MQ=25)
                       cgggggg.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATc                                                                              <  2:1023372/99‑1 (MQ=25)
                       cgggggg.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATc                                                                              <  2:237318/99‑1 (MQ=25)
                       cgggggg.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTACTTTCATCTTGCCCG.AAATATc                                                                              >  2:1100723/3‑101 (MQ=21)
                         ggggg.CGCTGCCCCCTCTT.GGC..CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGC.AAATATCCCgggggg                                                                      <  2:4231871/101‑2 (MQ=0)
                         ggggg.CGCTGCCCCCTCTT.GGC..CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGC.AAATATCCCgggggg                                                                      <  2:1478495/101‑2 (MQ=2)
                         ggggg.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATccc                                                                            <  2:2453882/101‑1 (MQ=25)
                         ggggg.CGCTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATccc                                                                            <  1:2137791/101‑1 (MQ=25)
                             g.cgcTGCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCgggg                                                                        >  1:1121439/1‑101 (MQ=37)
                                   gCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGGCCG.AAATATCCCGGGGGAGcc                                                                   >  2:4973558/1‑101 (MQ=25)
                                   gCCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGcc                                                                   <  2:4036376/101‑1 (MQ=37)
                                         ccctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCt                                                               <  1:2645069/91‑1 (MQ=255)
                                          cctcctggccTTCGCCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCtt                                                              <  1:3930720/92‑1 (MQ=37)
                                             cttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTgcg                                                           >  1:3232992/7‑101 (MQ=255)
                                             cttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGCAGCCGGCTTccg                                                           >  2:2502701/7‑101 (MQ=37)
                                                ggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTTCCCG.AAATATCCCGGGGGAGCCCGCTTgcgggc                                                        >  1:2724557/4‑101 (MQ=255)
                                                ggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTgcgggc                                                        >  2:2538902/4‑101 (MQ=255)
                                                   cTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGcgggcggg                                                     >  2:4518154/1‑101 (MQ=255)
                                                   cTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGcgggcggg                                                     >  1:3400121/1‑101 (MQ=255)
                                                    tgcGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCgggg                                                    <  2:37821/99‑1 (MQ=255)
                                                     tCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCggggg                                                   >  1:2669553/1‑101 (MQ=255)
                                                      cGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                  <  1:4162774/101‑1 (MQ=255)
                                                        ggcAAATCCCCCCCCGGGGTTTTTTCGGGAAAAAAAAAAAGGGTTTTTTCTTTCCTTTTTCCCGAAAATTCCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                                 <  1:1020567/99‑1 (MQ=255)
                                                        gCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAg                                                <  2:4848740/101‑1 (MQ=255)
                                                            aTTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                            >  1:1842656/1‑101 (MQ=255)
                                                             ttCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccc                                           >  2:1581810/1‑101 (MQ=255)
                                                              tCACCCCCCGGCATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAGCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                                          <  2:3964271/101‑1 (MQ=255)
                                                                gccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCg                                        <  1:554365/100‑1 (MQ=255)
                                                                aCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCg                                        <  2:3232741/101‑1 (MQ=255)
                                                                 ccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGa                                       <  2:1654836/101‑1 (MQ=255)
                                                                       gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                 >  1:3596420/1‑101 (MQ=255)
                                                                       gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                 >  2:3553740/1‑101 (MQ=255)
                                                                        ggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGccc                                >  2:3162466/1‑101 (MQ=255)
                                                                         gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                               <  1:4666918/101‑1 (MQ=255)
                                                                          atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCgc                              >  2:733843/1‑100 (MQ=255)
                                                                          atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCCCCCGa                              >  2:1511156/1‑101 (MQ=255)
                                                                           tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                             >  2:3867473/1‑101 (MQ=255)
                                                                           tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                             >  2:2717608/1‑101 (MQ=255)
                                                                           tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                             >  1:149204/1‑101 (MQ=255)
                                                                           tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                             >  2:3366566/1‑101 (MQ=255)
                                                                            atTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                            >  2:3639854/1‑101 (MQ=255)
                                                                            atTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                            <  2:3509415/101‑1 (MQ=255)
                                                                              ttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg                          <  1:195066/101‑1 (MQ=255)
                                                                                cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg                        >  1:2528990/1‑101 (MQ=255)
                                                                                 gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                       >  1:698429/1‑101 (MQ=255)
                                                                                  ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                      <  1:3670577/101‑1 (MQ=255)
                                                                                  ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                      <  1:3030695/101‑1 (MQ=255)
                                                                                    cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                    >  1:1903281/1‑101 (MQ=255)
                                                                                    cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                    >  1:2312313/1‑101 (MQ=255)
                                                                                     aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                   >  2:88232/1‑101 (MQ=255)
                                                                                      aGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGc                  >  1:3104665/1‑101 (MQ=255)
                                                                                       gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt                 >  2:2167219/1‑101 (MQ=255)
                                                                                       gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt                 >  2:2084809/1‑101 (MQ=255)
                                                                                        aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                >  1:3296221/1‑101 (MQ=255)
                                                                                        aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCCAAGCGCCCGACGAGCGGCGGGGtt                >  2:3006850/1‑101 (MQ=255)
                                                                                        aTAAAGAAGGTCTTTTCCTTTCATCTTACACG.AACTGTCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                <  2:4754042/101‑1 (MQ=255)
                                                                                          aaaGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc              >  1:2442162/1‑101 (MQ=255)
                                                                                          aaaGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc              >  2:4333584/1‑101 (MQ=255)
                                                                                           aagaagGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc             >  2:2240128/1‑101 (MQ=255)
                                                                                           aagaagGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc             >  1:2834749/1‑101 (MQ=255)
                                                                                               agGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgcc         <  2:3057205/101‑1 (MQ=255)
                                                                                                ggTTTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgccc        <  1:4706496/101‑1 (MQ=255)
                                                                                                  tCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa      >  2:4479791/1‑101 (MQ=255)
                                                                                                   cTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGGAGCGCCCCCGAAGCGCCCCACGCCCCGCGGGGTTTCCCCCCCAag     >  2:4505076/1‑101 (MQ=37)
                                                                                                   cTTTTCCTTTCATCTTACCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag     <  2:4723505/101‑1 (MQ=255)
                                                                                                    ttttCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg    >  2:4493705/1‑101 (MQ=255)
                                                                                                     tttCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga   >  1:803603/1‑101 (MQ=255)
                                                                                                     tttCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga   >  1:4260663/1‑101 (MQ=255)
                                                                                                      ttCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag  >  2:1395306/1‑101 (MQ=255)
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CTGGCGCGCGCTCTGAGACGAAAC.GGGGG.CGCTGCCCCC.CTC.CTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAG  >  NC_014034/2566513‑2566709

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG