BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,566,610	T→C	2.4%	intergenic (+82/+112)	purD → / ← sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,566,610	0	T→C	2.4%	4.0 0.0 ‑0.1	205	intergenic (+82/+112)	purD/sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand): new base (2/3): ref base (100/100): total (102/103)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.89e-01

Only 100 of 231 total aligned reads displayed.


CTGGCGCGCGCTCTGAGACGAAAC.GGGGG.CGCTGCCCCCCTCCTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGG  >  NC_014034/2566513‑2566710


 tGGCGCGCGCTCTGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                                                                                      <  2:3994153/101‑1 (MQ=21)


 tGGCGCGCGCTCTGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                                                                                      <  2:1768660/101‑1 (MQ=21)


  ggCGCGCGCTCTGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                                                                                     <  2:4673142/101‑1 (MQ=21)


   gcgcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCt                                                                                                    <  2:2861298/101‑1 (MQ=21)


    cgcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                   <  1:226240/101‑1 (MQ=21)


      cgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                                 <  2:3302022/101‑1 (MQ=21)


      cgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                                 <  2:192013/101‑1 (MQ=21)


       gcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCa                                                                                                <  1:2952744/101‑1 (MQ=21)


        cgcTCTGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                                                                               <  1:3208980/101‑1 (MQ=21)


        cgcTCTGAGACGAAAC.GGGGGTCGCTTCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                                                                               <  1:1616734/101‑1 (MQ=25)


         gcTCTGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                              <  2:2891000/101‑1 (MQ=21)


         gcTCTGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                              <  1:2154159/101‑1 (MQ=21)


         gcTCTGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                              <  1:2565977/101‑1 (MQ=21)


          ctctGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTTATCt                                                                                             >  1:2119601/1‑101 (MQ=21)


          ctctGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCAGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                                                                                             <  2:1093383/101‑1 (MQ=21)


          ctctGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGAAATTTCGGGGCAGAAAAAAAAGGGCTTTTTCttttcttt                                                                                             >  1:899201/1‑96 (MQ=9)


           tctGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTCTCCTTTCATCtt                                                                                            <  1:2728105/101‑1 (MQ=21)


            ctGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                                                                           <  2:4898629/101‑1 (MQ=21)


             tGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGc                                                                                          <  1:4249700/101‑1 (MQ=21)


             tGAGACGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGc                                                                                          >  2:3223909/1‑101 (MQ=21)


              gagaCGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCCGGAGATTTTGGGGCAGATAAAAAAGGGGTTTTTCTTTTATTttttc                                                                                         >  1:3572030/1‑98 (MQ=2)


               agaCGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                                                                                        <  2:1699276/101‑1 (MQ=21)


               agaCGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                                                                                        <  1:1748694/101‑1 (MQ=21)


                gaCGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                                                                                       <  2:3407080/101‑1 (MQ=21)


                 aCGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.a                                                                                     <  2:539174/101‑1 (MQ=21)


                  cGAAACGGGGGG.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGACATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.aa                                                                                    >  2:613597/1‑101 (MQ=21)


                   gaagcgggggg.CGCAGCCCCCCTCTTGGCCATCGGCCAAGTCAGCCACAGGGATATATCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.aaa                                                                                   <  2:4183595/95‑1 (MQ=255)


                    aaacgggggg.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGAAATTCCGGCCAAAAAAAGAAAGGACTTTTCTTTTCTTCTTCCCCG.aaaa                                                                                  >  2:4359411/6‑100 (MQ=2)


                    aaacgggggg.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCCGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCC.AAat                                                                                  >  1:2033105/6‑101 (MQ=21)


                     ggcaggggg.CGCTG.CCCCCTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGC.AAATATCCCgg                                                                           >  1:4977195/5‑101 (MQ=0)


                     aacgggggg.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTATTTTCCTTTCATCTTGCCCG.AAata                                                                                 >  1:557794/5‑101 (MQ=21)


                       cgggggg.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATc                                                                               <  2:1023372/99‑1 (MQ=25)


                       cgggggg.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATc                                                                               >  2:3038455/3‑101 (MQ=25)


                         ggggg.CGCTG.CCCCCTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGC.AAATATCCCgggggg                                                                       >  2:1480212/1‑100 (MQ=0)


                          gggg.CCCTGCCCCCTTTTGGC.CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGC.AAATATCCCgggggga                                                                      <  2:359618/101‑3 (MQ=2)


                           ggg.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCgg                                                                           <  2:2353969/101‑1 (MQ=37)


                            gg.CGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCggg                                                                          >  2:2674843/1‑101 (MQ=25)


                             g.cgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCgggg                                                                         >  1:1121439/1‑101 (MQ=37)


                                   gCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGGCCG.AAATATCCCGGGGGAGcc                                                                    >  2:4973558/1‑101 (MQ=25)


                                   gCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGcc                                                                    <  2:4036376/101‑1 (MQ=37)


                                       ccctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCt                                                                <  1:2645069/91‑1 (MQ=255)


                                         ctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTg                                                              <  2:2205523/93‑1 (MQ=255)


                                         ctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTg                                                              <  1:3269055/93‑1 (MQ=255)


                                           cttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTgcg                                                            >  2:2497216/7‑101 (MQ=255)


                                             tggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCCTgcggg                                                          >  2:240400/5‑101 (MQ=255)


                                             tggccTTCGGCCAATTCACCCCCCGGGATAATTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AGATATCCCGGGGGAGCCCGCTTgcggg                                                          >  2:1612481/5‑101 (MQ=255)


                                                 cTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGcgggcggg                                                      <  1:4678742/101‑1 (MQ=255)


                                                 cTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGcgggcggg                                                      >  2:4518154/1‑101 (MQ=255)


                                                  tgcGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCgggg                                                     <  2:37821/99‑1 (MQ=255)


                                                   tCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCggggg                                                    >  1:2669553/1‑101 (MQ=255)


                                                    cGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                   <  1:4162774/101‑1 (MQ=255)


                                                      ggcAAATCCCCCCCCGGGGTTTTTTCGGGAAAAAAAAAAAGGGTTTTTTCTTTCCTTTTTCCCGAAAATTCCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                                  <  1:1020567/99‑1 (MQ=255)


                                                      gCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCATTTCATCTTGCCCG.AAATATCCCGGGGGGGCCCGCTTGGGGGAggggggga                                                 >  1:3076679/1‑98 (MQ=37)


                                                          aTTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGGGGGGGCAGCGcc                                             >  2:2375588/1‑101 (MQ=255)


                                                          aTTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                             >  1:1842656/1‑101 (MQ=255)


                                                          aTTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTTCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                             >  2:2525011/1‑101 (MQ=255)


                                                            tCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                                           >  1:3874599/1‑101 (MQ=255)


                                                             cACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                          >  1:3361587/1‑101 (MQ=255)


                                                             cACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                          >  2:2158328/1‑101 (MQ=255)


                                                                     gggATATTTCGGTCAATATAAAGAAGGTCTTTTCCTTTCTTCTTGCCCA.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCATCGCCCCCGAAGCGcc                                  <  1:1987380/101‑1 (MQ=255)


                                                                     gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATTTTGCCCG.AAATATCCCGGGGAAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                  >  1:857155/1‑101 (MQ=255)


                                                                      ggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGccc                                 >  2:3162466/1‑101 (MQ=255)


                                                                       gATATTTGGGGCAAGATAAGGAAGGTTTTTTCTTTTCATCTTGCCGG.AAATACCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                                <  2:22593/101‑1 (MQ=255)


                                                                        atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCgc                               >  2:733843/1‑100 (MQ=255)


                                                                        atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGa                               >  2:3544099/1‑101 (MQ=255)


                                                                         tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                              >  2:4480908/1‑101 (MQ=255)


                                                                         tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                              >  1:149204/1‑101 (MQ=255)


                                                                            ttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg                           >  1:3613787/1‑101 (MQ=255)


                                                                               gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                        >  1:466027/1‑101 (MQ=255)


                                                                               gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                        >  1:698429/1‑101 (MQ=255)


                                                                                ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                       <  1:3670577/101‑1 (MQ=255)


                                                                                 gCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCCTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggcg                      >  1:4711090/1‑101 (MQ=255)


                                                                                  cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                     >  1:2312313/1‑101 (MQ=255)


                                                                                  cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                     >  1:1903281/1‑101 (MQ=255)


                                                                                   aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                    >  2:88232/1‑101 (MQ=255)


                                                                                   aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                    >  2:4406318/1‑101 (MQ=255)


                                                                                    aGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGc                   >  1:3104665/1‑101 (MQ=255)


                                                                                    aGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGc                   >  1:650738/1‑101 (MQ=255)


                                                                                      aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                 >  1:2990843/1‑101 (MQ=255)


                                                                                      aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                 >  1:3296221/1‑101 (MQ=255)


                                                                                      aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                 >  2:484452/1‑101 (MQ=255)


                                                                                      aTAAAGAAGGTCTTTTCCTTTCATCTTACACG.AACTGTCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                 <  2:4754042/101‑1 (MQ=255)


                                                                                       tAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTg                <  2:1577258/101‑1 (MQ=255)


                                                                                       tAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTg                >  1:3944030/1‑101 (MQ=255)


                                                                                       tAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATACCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTg                <  2:3496729/101‑1 (MQ=255)


                                                                                        aaaGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc               >  2:3912354/1‑101 (MQ=255)


                                                                                        aaaGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc               >  2:4333584/1‑101 (MQ=255)


                                                                                         aagaagGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc              >  1:2454976/1‑101 (MQ=255)


                                                                                         aagaagGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc              >  1:2834749/1‑101 (MQ=255)


                                                                                                 cTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGGAGCGCCCCCGAAGCGCCCCACGCCCCGCGGGGTTTCCCCCCCAag      >  2:4505076/1‑101 (MQ=37)


                                                                                                 cTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag      >  2:3847062/1‑101 (MQ=255)


                                                                                                 cTTTTCCTTTCATCTTACCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag      <  2:4723505/101‑1 (MQ=255)


                                                                                                  ttttCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCAGACGCGCGGCGGGCTTGCCCGCCCAagg     >  2:2041820/1‑101 (MQ=255)


                                                                                                   tttCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCNCGACGCGCGGCGGGGCTGCCCGCCCAaggg    >  1:4883707/1‑100 (MQ=255)


                                                                                                   tttCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga    >  1:4260663/1‑101 (MQ=255)


                                                                                                   tttCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga    >  1:803603/1‑101 (MQ=255)


                                                                                                    ttCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag   >  2:1330689/1‑101 (MQ=255)


                                                                                                    ttCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag   >  2:1395306/1‑101 (MQ=255)


                                                                                                     tCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg  >  1:3976060/1‑101 (MQ=255)


                                                                                                     tCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg  <  1:4481228/101‑1 (MQ=255)


CTGGCGCGCGCTCTGAGACGAAAC.GGGGG.CGCTGCCCCCCTCCTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGG  >  NC_014034/2566513‑2566710

Base quality scores:


ATCG

< 3 ≤


ATCG

< 16 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG