Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,046 G→T 9.8% intergenic (+92/+84) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0460G→T 9.8% 66.4 ‑0.2 ‑1.5295intergenic (+92/+84)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (14/14):  ref base (134/124):  total (157/138)
Fisher's exact test strand distribution p-value = 8.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.82e-02

Only 100 of 318 total aligned reads displayed.

GAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGA  >  NC_014034/3003946‑3004146
                                                                                                     |                                                                                                    
gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                                      <  1:4224806/101‑1 (MQ=255)
 agaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGc                                                                                                     <  2:1309004/101‑1 (MQ=255)
 agaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGc                                                                                                     >  1:3898431/1‑101 (MQ=255)
 agaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCCGGATATTTGGACCAAGGTGAAAGAAG.ATCCCGGGTTGc                                                                                                     >  1:1130023/1‑101 (MQ=255)
  gaTCTGAGGGGAAGGCGTGTGGCGGCGCCCCCCTCATGCCCTTACGGCCAATTCACCCCCCGGGATATTTAGAGCAAGGGTAAAGCGG.ATACCGGCTTGcc                                                                                                    >  2:2201719/1‑101 (MQ=12)
   aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                                   <  1:3270095/101‑1 (MQ=255)
     cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCttt                                                                                                 <  2:1047715/101‑1 (MQ=255)
     cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCttt                                                                                                 >  2:568875/1‑101 (MQ=255)
      tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                                <  1:1425026/101‑1 (MQ=255)
       gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                               <  1:2985972/101‑1 (MQ=255)
       gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                               >  1:2230103/1‑101 (MQ=255)
       gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                               <  1:454493/101‑1 (MQ=255)
        aGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAc                                                                                              <  1:788213/101‑1 (MQ=255)
         gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAcc                                                                                             <  1:4457989/101‑1 (MQ=255)
           gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                                           <  1:2295134/101‑1 (MQ=255)
            cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAT.ATCCCGGTTTGCCTTTCACCTTg                                                                                          <  2:1645289/101‑1 (MQ=255)
            cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                                          >  1:2412265/1‑101 (MQ=255)
            cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                                          <  1:264199/101‑1 (MQ=255)
               ggCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcg                                                                                       <  2:3732028/101‑1 (MQ=255)
               ggCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCCGGGTATTTTGACCAAGGGGGAAACAG.AAACCCGGTTGCCCTTCACCCTTgcg                                                                                       >  1:1708651/1‑101 (MQ=16)
                  ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCGCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaa                                                                                    <  1:1658579/101‑1 (MQ=255)
                   gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                                   >  1:4025268/1‑101 (MQ=255)
                   gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                                   >  2:810863/1‑101 (MQ=255)
                    ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                  >  2:2358988/1‑101 (MQ=255)
                      ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                <  1:968306/101‑1 (MQ=255)
                      ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                <  2:4732737/101‑1 (MQ=255)
                       gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                               <  2:406364/101‑1 (MQ=255)
                       gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                               <  2:2602618/101‑1 (MQ=255)
                       gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                               <  2:3589046/101‑1 (MQ=255)
                        cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                              <  2:451383/101‑1 (MQ=255)
                         gcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATccc                                                                             <  2:1625137/101‑1 (MQ=255)
                         gcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTTGCGCAAATATccc                                                                             >  1:3718069/1‑101 (MQ=255)
                           tGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                           <  1:41756/101‑1 (MQ=255)
                           tGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCCGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGGTTGCCCTTCACCTTTGCGCAAATATccacg                                                                           >  2:4319864/1‑98 (MQ=25)
                            gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGGCTTTCACCTTGGCGCAAATATCCCggg                                                                          >  2:1376472/1‑101 (MQ=255)
                            gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                          <  1:1639371/101‑1 (MQ=255)
                             ccccccTCTTGGCCT.TCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                                                   <  1:4745535/101‑6 (MQ=37)
                              cccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggggg                                                                        <  2:2344984/101‑1 (MQ=255)
                                   tctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                                   <  1:2359152/101‑1 (MQ=255)
                                   tctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                                   <  2:174158/101‑1 (MQ=255)
                                    ctTGGCCTGCCGGCCAATTCCCCCCCCGGGATAGTTGGACCAAGGTGAAAGCAG.CTCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCCGGGGGAGTcc                                                                  <  1:4367089/101‑1 (MQ=25)
                                    ctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTcc                                                                  <  2:2958657/101‑1 (MQ=255)
                                     ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                                 <  2:3930688/101‑1 (MQ=255)
                                      tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                                <  1:3774155/101‑1 (MQ=255)
                                      tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                                >  2:156278/1‑101 (MQ=255)
                                      tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                                >  1:333130/1‑101 (MQ=255)
                                        gCCTGACGGCCAATTCACCCCCCGGGATATTTTGACCAAGGGGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCCAATATCCCGGGGGGGGTCCcccg                                                              >  1:1032845/1‑101 (MQ=25)
                                        gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgcg                                                              <  1:4624015/101‑1 (MQ=255)
                                        gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgcg                                                              >  2:3102249/1‑101 (MQ=255)
                                         ccTGACGGCCAATTCACCCCCCGGGTCATTTGGACCAAGGCGAAAGCAG.ATCCCGGTTTGCCATTCACCTTCGCGCAAATAACCCGGGGGGGGCCCCGCgg                                                             >  1:2733077/1‑101 (MQ=255)
                                          cTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCggg                                                            >  2:2390908/1‑101 (MQ=255)
                                          cTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCggg                                                            >  2:299214/1‑101 (MQ=255)
                                           tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                           >  1:1516849/1‑101 (MQ=255)
                                             aCGGCCAATTCCCCCCCGGGGATATTGGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTGGGCGAAAATACCCCGGGGGGAGTCCCGCGGGGAc                                                         <  1:1771799/101‑1 (MQ=21)
                                             aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                         >  2:71604/1‑101 (MQ=255)
                                              cGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATCTCCCGGGGGGAGTCCCGCGGGGACg                                                        <  1:289254/101‑1 (MQ=255)
                                               ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                       <  2:3511420/101‑1 (MQ=255)
                                                gCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggg                                                      >  1:2123343/1‑101 (MQ=255)
                                                  cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                    >  2:3024614/1‑101 (MQ=255)
                                                    aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggggg                                                  >  2:2154822/1‑100 (MQ=255)
                                                         ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                             >  2:3466873/1‑101 (MQ=18)
                                                           ccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccc                                           >  2:4675800/1‑101 (MQ=18)
                                                             ccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                         <  1:2530452/101‑1 (MQ=17)
                                                             ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                         >  1:2460884/1‑101 (MQ=255)
                                                              cGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                        <  2:2832019/101‑1 (MQ=17)
                                                               gggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccc                                       >  1:2173829/1‑100 (MQ=18)
                                                               gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                       >  2:4910091/1‑101 (MQ=255)
                                                                 gATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctc                                     >  2:1171050/1‑98 (MQ=18)
                                                                 gATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctc                                     >  1:492849/1‑98 (MQ=18)
                                                                 gATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctc                                     >  2:3204991/1‑98 (MQ=18)
                                                                 gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                     >  2:3828208/1‑101 (MQ=255)
                                                                  atatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                    >  1:5046354/1‑97 (MQ=18)
                                                                   tatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                   >  1:4081421/1‑96 (MQ=18)
                                                                   tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                   >  1:3550677/1‑101 (MQ=255)
                                                                    atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                  >  2:2487914/1‑95 (MQ=18)
                                                                    atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                  <  2:2229948/101‑1 (MQ=255)
                                                                    atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                  >  1:464543/1‑101 (MQ=255)
                                                                    atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCAGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                  >  1:1898578/1‑101 (MQ=255)
                                                                       tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                               >  1:3391738/1‑101 (MQ=255)
                                                                       tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                               >  1:1970427/1‑101 (MQ=255)
                                                                       tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                               >  1:704175/1‑101 (MQ=255)
                                                                         gACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                             <  1:614998/101‑1 (MQ=255)
                                                                          aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                            >  1:1261264/1‑101 (MQ=255)
                                                                              aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                        >  2:2881731/1‑101 (MQ=255)
                                                                              aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                        <  2:2729242/101‑1 (MQ=255)
                                                                                gTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                      >  1:4126718/1‑101 (MQ=255)
                                                                                  gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg                    >  1:3194271/1‑101 (MQ=255)
                                                                                  gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg                    >  1:4294137/1‑101 (MQ=255)
                                                                                     aGCAG.ATCCCGGTTTGCCTTTCGCCTTCGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTc                 <  2:5045843/101‑1 (MQ=255)
                                                                                      gCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                <  2:3708487/101‑1 (MQ=255)
                                                                                      gCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                <  2:2121435/101‑1 (MQ=255)
                                                                                       cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg               >  2:1917209/1‑101 (MQ=255)
                                                                                       cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg               >  2:3130292/1‑101 (MQ=255)
                                                                                           aTCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCg            >  2:4137008/1‑101 (MQ=255)
                                                                                             cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg          >  2:648491/1‑101 (MQ=255)
                                                                                                ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt       <  1:3095584/101‑1 (MQ=255)
                                                                                                 gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg      <  2:3378582/101‑1 (MQ=255)
                                                                                                  tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg     >  2:4815748/1‑101 (MQ=255)
                                                                                                   ttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc    >  1:1325135/1‑101 (MQ=255)
                                                                                                     gCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa  >  2:786867/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGA  >  NC_014034/3003946‑3004146

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG