Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 317,927 A→G 100% K338E (AAG→GAG)  RCAP_rcc00280 → hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034317,9270A→G100.0% 1013.8 268K338E (AAG→GAG) RCAP_rcc00280hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (144/124):  ref base (0/0):  total (144/124)

Only 100 of 274 total aligned reads displayed.

AAGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCAAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATCCGC  >  NC_014034/317829‑318024
                                                                                                  |                                                                                                 
 aGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAgg                                                                                                <  1:3494000/101‑1 (MQ=255)
    gACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAtg                                                                                             >  1:1270125/1‑101 (MQ=255)
        cTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGct                                                                                         >  2:4920322/1‑101 (MQ=255)
         tGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGctc                                                                                        <  2:771611/101‑1 (MQ=255)
          ggAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGctct                                                                                       <  1:3799742/101‑1 (MQ=255)
           gAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCtt                                                                                      <  1:61732/101‑1 (MQ=255)
            aGCACGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTg                                                                                     <  2:3176478/101‑1 (MQ=255)
             gCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACTGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGa                                                                                    <  2:204955/101‑1 (MQ=255)
               ccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATc                                                                                  <  2:2310599/101‑1 (MQ=255)
                  cgacgaCCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTGGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACg                                                                               <  1:2996219/101‑1 (MQ=255)
                  cgacgaCCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACg                                                                               >  2:1842828/1‑101 (MQ=255)
                  cgacgaCCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACg                                                                               <  1:3269309/101‑1 (MQ=255)
                     cgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGccc                                                                            <  2:4395212/101‑1 (MQ=255)
                      gacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCg                                                                           <  2:3519186/101‑1 (MQ=255)
                      gacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATATGCGCCCCGAGGATGTGCTCTTGATCACGCCCg                                                                           >  1:4384118/1‑101 (MQ=255)
                         cGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAgg                                                                        >  1:985618/1‑101 (MQ=255)
                           aGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGaa                                                                      <  1:4847588/101‑1 (MQ=255)
                            gggCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAAt                                                                     >  1:1966181/1‑101 (MQ=255)
                             ggCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAAtt                                                                    >  1:2279207/1‑101 (MQ=255)
                              gCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTc                                                                   <  1:132699/101‑1 (MQ=255)
                               cAATGTCTGGATCACGATGAACGGGGTGCATCTGGCCGAGGGGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTATTGATCACGCCCGAGGAATTCg                                                                  >  1:4414875/1‑101 (MQ=255)
                                aaTGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCgg                                                                 <  2:1018762/101‑1 (MQ=255)
                                  tGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcg                                                               >  1:2896733/1‑101 (MQ=255)
                                  tGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcg                                                               >  2:1706634/1‑101 (MQ=255)
                                  tGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcg                                                               >  2:2644898/1‑101 (MQ=255)
                                   gTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcgc                                                              >  2:3186206/1‑101 (MQ=255)
                                     cTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCa                                                            <  2:1573702/101‑1 (MQ=255)
                                     cTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCa                                                            <  1:3892040/101‑1 (MQ=255)
                                      tGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCAt                                                           <  2:1307777/101‑1 (MQ=255)
                                       ggATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATc                                                          <  2:4250102/101‑1 (MQ=255)
                                        gATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCt                                                         <  1:2726071/101‑1 (MQ=255)
                                        gATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCt                                                         >  2:3676782/1‑101 (MQ=255)
                                         aTCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTg                                                        <  2:2907578/101‑1 (MQ=255)
                                          tCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGa                                                       >  1:4174255/1‑101 (MQ=255)
                                          tCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGa                                                       >  2:3467208/1‑101 (MQ=255)
                                           cACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAc                                                      <  1:469029/101‑1 (MQ=255)
                                            aCGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAcc                                                     >  2:4641324/1‑101 (MQ=255)
                                               cTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCggg                                                  >  1:2558879/1‑101 (MQ=255)
                                                 gAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGcc                                                >  2:3403793/1‑101 (MQ=255)
                                                 gAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGcc                                                >  1:1053611/1‑101 (MQ=255)
                                                 gAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGcc                                                >  2:4165726/1‑101 (MQ=255)
                                                  aaCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACGGGGCCt                                               >  1:1191859/1‑101 (MQ=255)
                                                  aaCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGAATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCt                                               <  1:4274320/101‑1 (MQ=255)
                                                    cGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTgg                                             >  2:2968105/1‑101 (MQ=255)
                                                    cGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCACCGAGGATCTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTgg                                             >  2:252402/1‑101 (MQ=255)
                                                       ggTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGc                                          >  2:203579/1‑101 (MQ=255)
                                                        gTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCt                                         >  1:5008896/1‑101 (MQ=255)
                                                        gTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCt                                         >  1:4610771/1‑101 (MQ=255)
                                                        gTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCt                                         >  1:2097567/1‑101 (MQ=255)
                                                         tGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCtt                                        >  2:2171560/1‑101 (MQ=255)
                                                         tGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCtt                                        >  1:183270/1‑101 (MQ=255)
                                                          gCATCTGGCCGAGGTGGTCGATGCGAGCGATCTGCGCCCCGCGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTc                                       >  2:458065/1‑101 (MQ=255)
                                                           cATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTcc                                      <  1:3153544/101‑1 (MQ=255)
                                                             tCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCt                                    <  2:2828786/101‑1 (MQ=255)
                                                             tCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCt                                    >  2:1902834/1‑101 (MQ=255)
                                                              cTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCtt                                   <  2:473543/101‑1 (MQ=255)
                                                              cTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAGGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCtt                                   >  1:4034728/1‑101 (MQ=255)
                                                               tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  2:199591/101‑1 (MQ=255)
                                                               tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  2:3847124/101‑1 (MQ=255)
                                                               tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  1:3071166/101‑1 (MQ=255)
                                                               tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  1:2595614/101‑1 (MQ=255)
                                                               tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  1:2334232/101‑1 (MQ=255)
                                                                ggCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTg                                 <  2:2688942/101‑1 (MQ=255)
                                                                ggCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTg                                 >  1:2455092/1‑101 (MQ=255)
                                                                 gCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGc                                >  2:3981433/1‑101 (MQ=255)
                                                                 gCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGc                                <  1:4201570/101‑1 (MQ=255)
                                                                   cGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGccc                              <  1:1007478/101‑1 (MQ=255)
                                                                    gAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGcccc                             <  1:3350411/101‑1 (MQ=255)
                                                                    gAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGcccc                             >  1:4494423/1‑101 (MQ=255)
                                                                    gAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGcccc                             <  1:701833/101‑1 (MQ=255)
                                                                     gggtggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCg                            <  2:1767971/100‑1 (MQ=255)
                                                                     aGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCg                            <  2:215279/101‑1 (MQ=255)
                                                                     aGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCg                            >  2:1088521/1‑101 (MQ=255)
                                                                       gtggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCt                          <  2:3342186/101‑1 (MQ=255)
                                                                       gtggtCGATGCGGGCGATCTGAGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCt                          >  1:3728497/1‑101 (MQ=255)
                                                                          gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtttt                       <  2:2793051/101‑1 (MQ=255)
                                                                          gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtttt                       <  2:1418748/101‑1 (MQ=255)
                                                                            cGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTcc                     >  1:3106350/1‑101 (MQ=255)
                                                                             gATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCg                    >  2:3132758/1‑101 (MQ=255)
                                                                             gATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCg                    >  2:645232/1‑101 (MQ=255)
                                                                               tGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCt                  <  1:83998/101‑1 (MQ=255)
                                                                                 cGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaa                <  1:1815878/101‑1 (MQ=255)
                                                                                   ggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAc              >  1:206081/1‑101 (MQ=255)
                                                                                   ggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAc              >  2:758515/1‑101 (MQ=255)
                                                                                   ggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAc              <  1:3199437/101‑1 (MQ=255)
                                                                                      gATCTGCTCCCAGAGGATGTGCTCTTTATCGCGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgcc           <  2:1191742/101‑1 (MQ=255)
                                                                                       aTCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccg          >  1:2800342/1‑101 (MQ=255)
                                                                                       aTCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccg          >  2:3203517/1‑101 (MQ=255)
                                                                                       aTCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccg          <  1:1534826/101‑1 (MQ=255)
                                                                                       aTCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGCGGCTTCACTTTGCCCCGCTTTTCCGCTAAAcgccg          >  2:5013053/1‑101 (MQ=255)
                                                                                       aTCTGCGCCCCGAGGATGTGCTCTTGATCACACCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccg          >  1:2177794/1‑101 (MQ=255)
                                                                                         cTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACgccgcc        >  2:3615348/1‑101 (MQ=255)
                                                                                         cTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACgccgcc        <  2:2708610/101‑1 (MQ=255)
                                                                                          tGCGCCGCGAGGATGTGCTCTTGATCACGCCCGTGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCa       <  1:4325087/101‑1 (MQ=255)
                                                                                           gcgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCAt      >  2:4017124/1‑101 (MQ=255)
                                                                                           gcgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCAt      <  2:1440279/101‑1 (MQ=255)
                                                                                            cgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATc     >  1:2832014/1‑101 (MQ=255)
                                                                                             gcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATcc    >  1:1380324/1‑101 (MQ=255)
                                                                                             gcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATcc    <  2:4483291/101‑1 (MQ=255)
                                                                                             gcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATcc    <  1:4741953/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AAGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCAAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATCCGC  >  NC_014034/317829‑318024

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG