BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,254,694	A→C	100%	intergenic (+19/‑164)	glpX → / → recJ	fructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,254,694	0	A→C	100.0%	423.0	126	intergenic (+19/‑164)	glpX/recJ	fructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand): new base (7/114): ref base (0/0): total (12/114)

Only 100 of 150 total aligned reads displayed.


TCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTGCCGACAGG  >  NC_014034/3254599‑3254788


tCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.ATGc.ccg                                                                                                >  2:1595029/1‑99 (MQ=255)


    gaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.Tcccc                                                                                           <  2:3744939/101‑1 (MQ=255)


     aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGG.CGGC.CG.Tccccc                                                                                          >  2:2000155/1‑101 (MQ=255)


     aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.Tccccc                                                                                          <  1:1163313/101‑1 (MQ=255)


     aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.Tccccc                                                                                          <  1:4123047/101‑1 (MQ=255)


       cGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGACTG..CG.GCCCCCGc                                                                                        >  2:4131950/1‑101 (MQ=255)


             gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCAGGGGGTCTGC.CT.TCCCCCGCAccc                                                                                    >  2:4503104/1‑101 (MQ=255)


             gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCAccccc                                                                                  <  2:650785/101‑1 (MQ=255)


              aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCt                                                                                 <  2:1172477/101‑1 (MQ=255)


              aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCt                                                                                 <  2:3536668/101‑1 (MQ=255)


               accTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTg                                                                                <  1:1139185/100‑1 (MQ=255)


                ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACACGGGG.CTGCGCG.TCCCCCCCCccccccc                                                                                >  1:300974/1‑99 (MQ=255)


                 cTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTgcg                                                                              <  2:1225473/101‑1 (MQ=255)


                  tGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTgcgc                                                                             <  2:2077008/101‑1 (MQ=255)


                  tGATGCGCCCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTgcgc                                                                             <  1:4511704/101‑1 (MQ=255)


                  ggATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTgcgc                                                                             <  1:984804/100‑1 (MQ=255)


                   gATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGC.CG.CCCCCCCCCcccccccccc                                                                             >  1:3883461/1‑96 (MQ=255)


                    aTGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGG.CTGC.CG.TCCCCCCCCcccccccccccc                                                                           >  2:602535/1‑94 (MQ=255)


                    aTGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGt                                                                           <  1:2511198/101‑1 (MQ=255)


                    aTGCGCCCGAAACCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGt                                                                           <  2:1761916/101‑1 (MQ=255)


                     tGCGCTCGAAACCCGACTCGGTGCGCCGCGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACACCCTGCGCGTa                                                                          <  2:3380724/101‑1 (MQ=255)


                       cgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.tt                                                                       <  2:3757239/101‑1 (MQ=255)


                        tcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.ttt                                                                      <  2:113710/100‑1 (MQ=255)


                        gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.ttt                                                                      <  1:2454896/101‑1 (MQ=255)


                        gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.ttt                                                                      <  2:1502133/101‑1 (MQ=255)


                        gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.ttt                                                                      <  1:4590681/101‑1 (MQ=255)


                        cctggAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.ttt                                                                      <  1:2645827/97‑1 (MQ=255)


                         cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.tttt                                                                     <  2:3191954/101‑1 (MQ=255)


                          ccgaaccccGGCTCGGTGGGGCGGGGGATCTATCGCAGCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTg                                                                    <  2:2678738/94‑1 (MQ=255)


                           cGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGAATGC.CG.TCCCCCCCACCCCCTTCGCGTT.tttttt                                                                   >  2:1690751/1‑99 (MQ=255)


                            gAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGcc                                                                  <  1:3810092/101‑1 (MQ=255)


                             aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GCGC.CC.CCCCCCGCCCCCCCCCCGCGCG.ttttttca                                                                 >  2:154999/1‑97 (MQ=255)


                             aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCa                                                                 <  2:3264212/101‑1 (MQ=255)


                              aaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCaa                                                                <  1:4095279/101‑1 (MQ=255)


                               aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAg                                                               <  1:2985918/101‑1 (MQ=255)


                               aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAg                                                               <  1:2363306/101‑1 (MQ=255)


                                 ccGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.a                                                            <  1:2508353/101‑1 (MQ=38)


                                   ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGC.CG.TCCCCCCCCCCCCCTGCGCGGA.TTTTTTCAAGG.aaa                                                          >  2:2400507/1‑101 (MQ=255)


                                   ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.aaa                                                          <  2:4354334/101‑1 (MQ=38)


                                   ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.aaa                                                          <  2:52710/101‑1 (MQ=38)


                                   ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.aaa                                                          <  1:881615/101‑1 (MQ=38)


                                      tCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACg                                                       <  1:3459689/101‑1 (MQ=38)


                                       cGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGC.GG.GCCCCCCCCCCCCCCCCCCCCC.TTTTGCCCAGG.aaaaag                                                       >  2:4288134/1‑99 (MQ=255)


                                       cGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GGGC.CG.CCCCCCCCCCCCCCCCCCCGTC.TTTTTTCCACG.AAAAccg                                                      >  1:3132371/1‑99 (MQ=255)


                                        ggTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.GAAACggg                                                     >  2:4686994/1‑101 (MQ=38)


                                        ggTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCAACCCCTGCGCGTA.TTTTGCCAAGG.AAAACggg                                                     <  2:3428920/101‑1 (MQ=38)


                                             gcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGC.CG.GCCCCCCCACCCCCCGCGCGGA.TTTTGCCCAGGAAAAACGGGGcgg                                                 >  2:1351487/1‑99 (MQ=255)


                                              ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGC.GGCCCCCCCCCCCCCCCCGCGCGT..TTTTTCCAGGA.AAAAAGGGGcggg                                                >  1:3701196/1‑98 (MQ=255)


                                                gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTc                                             <  2:1329646/101‑1 (MQ=38)


                                                gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTc                                             <  1:4202535/101‑1 (MQ=38)


                                                gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTc                                             <  1:35759/101‑1 (MQ=38)


                                                gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTc                                             <  1:4681595/101‑1 (MQ=38)


                                                 ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCa                                            <  2:2651208/101‑1 (MQ=38)


                                                 ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCa                                            <  1:1336623/101‑1 (MQ=38)


                                                 ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCa                                            <  2:2651129/101‑1 (MQ=38)


                                                 ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCa                                            <  2:4883215/101‑1 (MQ=38)


                                                 ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCa                                            <  2:2357809/101‑1 (MQ=38)


                                                  gTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGTGGC.GG.CCCCCCCCCCCCCCCCCCCCCT.TTTTCCCAGGC.AAAAAGGGGCGGtttac                                            >  1:740662/1‑99 (MQ=255)


                                                   tGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCAcc                                          <  1:606383/101‑1 (MQ=38)


                                                    gATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCt                                         <  2:4809995/101‑1 (MQ=38)


                                                      tCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGC.CG.TCCCCCCCACCCCCCGCGCGTATTTTTCCCAGGG.AAAACGGGG.CGTTTCACCCTg                                       >  2:4795371/1‑101 (MQ=255)


                                                       cTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTgg                                      <  1:1855781/101‑1 (MQ=38)


                                                         aTCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGCCTGC.GG.CCCCCCCCCCCCCCTCCGCGTC.TTTGGCACAGG.AAACGGGGGCCGttttccttcggg                                     >  1:1260766/1‑92 (MQ=255)


                                                          tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..Cg                                 <  2:40796/101‑1 (MQ=38)


                                                            gCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGaa                               <  1:4940112/101‑1 (MQ=38)


                                                             cAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGc..cagaa                              <  2:1659075/101‑5 (MQ=38)


                                                             cAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGaaa                              <  1:716970/101‑1 (MQ=38)


                                                              aaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAat                             <  2:2513724/101‑1 (MQ=38)


                                                              aaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAat                             <  1:3371963/101‑1 (MQ=38)


                                                              aaCCCCATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAat                             <  1:1729029/101‑1 (MQ=38)


                                                                cccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAatat                           <  1:2126111/101‑1 (MQ=38)


                                                                cccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAatat                           <  2:775468/101‑1 (MQ=38)


                                                                cccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAatat                           <  2:1221725/101‑1 (MQ=38)


                                                                cccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAatat                           <  1:4595005/101‑1 (MQ=38)


                                                                 ccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.c                         <  2:5001863/101‑1 (MQ=38)


                                                                 ccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.c                         <  1:4917796/101‑1 (MQ=38)


                                                                  cGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.cc                        <  1:5029297/101‑1 (MQ=38)


                                                                  cGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.cc                        <  2:4882179/101‑1 (MQ=38)


                                                                  cGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.cc                        <  2:4636215/101‑1 (MQ=38)


                                                                   gATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.ccc                       <  1:4585089/101‑1 (MQ=38)


                                                                    aTCCGCTGAGGCAACGCT.ACGGGGGG.GGGC.CGCCCCCCCCCCCCCCCCCCCCCCT.TTTTTCCAAGA.AAAAAGGGGCCGTTTCCCCTTGCC..CGAAAAAC.ccc                       >  1:504632/1‑101 (MQ=255)


                                                                           gAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGa               <  2:3361977/101‑1 (MQ=38)


                                                                           gAGGCAACGCT.AC.GGGGGACGGC.CG.CCCCCCGCACCCCCTGCGCGGC.TTTTTGCAAGG.AAAACAGGGGCGGTTCTCCTCGGCTGCGAAATA..CCAggggggg                >  2:3364709/1‑101 (MQ=255)


                                                                            aGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGaa              <  2:278420/101‑1 (MQ=38)


                                                                            aGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGaa              <  1:2753824/101‑1 (MQ=38)


                                                                            aGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGaa              <  1:2400681/101‑1 (MQ=38)


                                                                              gCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAAtt            <  1:2428872/101‑1 (MQ=38)


                                                                               cAACGCT.ACGGGGGG.CTGC.CG.GCCCCCCCCCCCCCCCCGCGGA.TTTTTCCAAGG.AAAACGGGGCCGGTTCACCTTTGG..CGCAAAATCCCCGGGGGGGAAtt            >  2:4565034/1‑101 (MQ=255)


                                                                               cAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAAttt           <  1:3816169/101‑1 (MQ=38)


                                                                                aaCGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTg          <  1:4687317/101‑1 (MQ=38)


                                                                                aaCGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTg          <  1:502706/101‑1 (MQ=38)


                                                                                   acgctacgggggACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTGc         <  2:2595796/94‑1 (MQ=38)


                                                                                   acgctacgggggACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTGc         <  2:3720797/94‑1 (MQ=38)


                                                                                   acgctacgggggACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTGc         <  2:4997551/94‑1 (MQ=38)


                                                                                    cgctacgggggACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTGcc        <  2:1822261/95‑1 (MQ=38)


                                                                                    cgctacgggggACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTGcc        <  2:3825448/95‑1 (MQ=38)


                                                                                        acgggggGCTGC.CG.TCCCCCGCACCCCCCGCGCGGA.TTTTGGCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGGATTTGCCGACa    >  2:1643346/3‑101 (MQ=37)


                                                                                         cgggggACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTGCCGACAg   <  1:1963716/100‑1 (MQ=255)


                                                                                         cgggggACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTGCCGACAg   <  1:3433183/100‑1 (MQ=255)


                                                                                         cgggggACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTGCCGACAg   <  2:3860812/100‑1 (MQ=255)


TCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTGCCGACAGG  >  NC_014034/3254599‑3254788

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG