BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,256,204	T→C	100%	C449C (TGT→TGC)	recJ →	single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,256,204	0	T→C	100.0%	460.3	126	C449C (TGT→TGC)	recJ	single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand): new base (54/72): ref base (0/0): total (54/72)

Only 100 of 135 total aligned reads displayed.


GCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGA.A.CC.GCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGG..CG.C.G.GCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256117‑3256293


gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGccc                                                                                            >  2:4043266/1‑101 (MQ=255)


gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGG.CACGGCTGGCCGA.GTTGCTCGccc                                                                                            <  2:2214537/101‑1 (MQ=255)


   tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                                                         >  1:4629387/1‑101 (MQ=255)


   tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                                                         <  1:1201644/101‑1 (MQ=255)


    ttGCTCAAGGGCTGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCCGCa                                                                                        >  1:4128457/1‑101 (MQ=255)


    ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGcc                                                                                        >  2:1292603/1‑100 (MQ=255)


     tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAg                                                                                       >  1:1374178/1‑101 (MQ=255)


      gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCCgg                                                                                      >  2:1347881/1‑101 (MQ=255)


      gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCAgg                                                                                      <  1:51266/101‑1 (MQ=255)


       cTCAAGGGCGTGGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCAggg                                                                                     <  2:920933/101‑1 (MQ=255)


        tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..c                                                                                  <  1:1870849/101‑1 (MQ=255)


         cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..cg                                                                                 >  1:3508661/1‑101 (MQ=255)


          aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..gg.c                                                                               >  2:2618333/1‑101 (MQ=255)


          aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCCGCCGGg..gg.c                                                                               >  2:1179403/1‑101 (MQ=255)


           aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGg..cggg                                                                               >  1:2526948/1‑99 (MQ=255)


             ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCCGGG..CG.C.g.gg                                                                          >  1:1062966/1‑100 (MQ=255)


              gcggcgGGCCCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.C.g.ggc                                                                         <  2:1572442/101‑3 (MQ=255)


                ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.C.g.ggcac                                                                       <  1:3410614/101‑5 (MQ=255)


                 gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.C.g.gggacg                                                                      >  2:1124347/1‑96 (MQ=255)


                 gcggGCACCGCATGGCCGCGGGTCTCACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.G.g.ggcacg                                                                      <  2:4859418/101‑6 (MQ=255)


                  cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGGTTGCCCCGCACGG..CG.CGG.GCA.c.gg                                                                    >  1:1559104/1‑101 (MQ=255)


                  cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.c.gg                                                                    <  2:4779617/101‑1 (MQ=255)


                    ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..GG.CGG.GCA.c.gggg                                                                  >  1:3980762/1‑99 (MQ=255)


                     gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.C.g.ggcccgggcg                                                                  >  1:737715/1‑92 (MQ=255)


                      cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.c.gggcgg                                                                <  1:314654/101‑5 (MQ=255)


                      cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.C.g.gggccgggggg                                                                 >  1:3083632/1‑91 (MQ=255)


                       aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.c.gggcggc                                                               <  1:1011539/101‑6 (MQ=255)


                         cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGcggccc                                                              <  1:4918924/101‑1 (MQ=255)


                         cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCCA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGcggccc                                                              <  2:4492720/101‑1 (MQ=255)


                           cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGcggcccgg                                                            <  2:3414345/101‑1 (MQ=255)


                             tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGcggcccggcc                                                          <  2:43663/101‑1 (MQ=255)


                              ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GGA.CGGGCGGGCCGGCCg                                                         >  2:2069807/1‑101 (MQ=255)


                              ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCg                                                         >  1:2145889/1‑101 (MQ=255)


                                ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGACC.CC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGAt                                                       <  1:2787319/101‑1 (MQ=255)


                                ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGAt                                                       <  2:3508391/101‑1 (MQ=255)


                                ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGAt                                                       <  2:1810577/101‑1 (MQ=255)


                                   cgGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCACC.GGCGGCCCGGCCCATCTg                                                    >  1:5013210/1‑101 (MQ=255)


                                   cgGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTg                                                    >  1:1526449/1‑101 (MQ=255)


                                    gggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGc                                                   <  1:751126/101‑1 (MQ=255)


                                    gggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCCGCCGATCTgg                                                   >  2:1363505/1‑100 (MQ=255)


                                     ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCg                                                  <  2:4682627/101‑1 (MQ=255)


                                     ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCg                                                  <  1:159393/101‑1 (MQ=255)


                                      gTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCgg                                                 <  1:3975962/101‑1 (MQ=255)


                                       tCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGAGG.TGCTCGCCCGGCAGGG..CG.C.GCGCACC.GGGGGGCCGGCCGCTTTGCCGc                                                >  2:3509414/1‑101 (MQ=255)


                                       tCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGc                                                >  2:4925906/1‑101 (MQ=255)


                                        cTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCt                                               >  1:1276473/1‑101 (MQ=255)


                                        cTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCt                                               >  1:1306090/1‑101 (MQ=255)


                                        cTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCCGCCCGTCTGCgggc                                               >  2:2808572/1‑99 (MQ=255)


                                         tGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCTGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCtt                                              <  2:1593756/101‑2 (MQ=255)


                                         tGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGGGGCCCCGCCGGTCTGCGGGTg                                              >  1:802149/1‑101 (MQ=255)


                                         tGACCCTGGCCGAGGAGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCCGGCGCTCTGCGGccg                                              >  1:4256371/1‑99 (MQ=255)


                                          gACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTgg                                             <  1:2616883/101‑1 (MQ=255)


                                             cctggCCGAGGCGCAGCTGGA.ACCC.GCGATGGCGC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACg                                          <  1:4331629/97‑1 (MQ=255)


                                                ggCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCC.CGGGGGGCCCGGCCCATCTGCGGCTGGGCGGGc                                       >  1:4870866/2‑101 (MQ=255)


                                                 gCCGAGGCGCAGCTGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                                      <  1:4542247/101‑1 (MQ=255)


                                                    gaggcgcagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                                   <  2:1411706/91‑1 (MQ=255)


                                                     gggcgcagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                  <  1:4546943/92‑1 (MQ=255)


                                                     aggcgcagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGGTCTGCGGCTGGGCGGGGCCCTg                                  >  2:1791275/10‑101 (MQ=255)


                                                     aggcgcagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                  <  2:4047142/92‑1 (MQ=255)


                                                      ggcgcagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                 <  2:4128140/93‑1 (MQ=255)


                                                       gcgcagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                <  1:2357771/94‑1 (MQ=255)


                                                       gcgcagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                <  1:4143007/94‑1 (MQ=255)


                                                       gcgcagctGGA.A.CCAGCGATGGCAG.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                <  2:4433272/94‑1 (MQ=255)


                                                        cgcagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGTCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                               <  2:158647/95‑1 (MQ=255)


                                                         gcagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGc                              >  2:2657273/6‑101 (MQ=255)


                                                         gcagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc                              <  1:3096687/96‑1 (MQ=255)


                                                          cagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGTAGGGCCCG.G.G.GAA.G.GGCGGTCCGGCCGATCTGCGGCTGGACGGGCTTCTGAAGcc                             >  2:2838301/5‑101 (MQ=255)


                                                          cagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CGCC.G.GCA.CGGGCGGCCCGGCCGATCTGCGGGTGGGACGGCTCCCTATGcc                             >  2:354160/5‑101 (MQ=255)


                                                          cagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             <  2:2801378/97‑1 (MQ=255)


                                                          cagctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGACGcc                             <  2:4279401/97‑1 (MQ=255)


                                                           gagctggatccc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             <  2:711062/91‑1 (MQ=255)


                                                           agctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCg                            >  1:4955129/4‑101 (MQ=255)


                                                           agctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCg                            >  1:1813104/4‑101 (MQ=255)


                                                             ctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGGGGGCTCCTGATGCCggg                          >  2:333202/2‑101 (MQ=255)


                                                             ctGGA.ACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGGTGCCggg                          >  1:1402532/2‑101 (MQ=255)


                                                                ggaaccc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGGGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCggggg                        >  2:2070732/6‑99 (MQ=255)


                                                                ggaaccc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcg                        >  1:3286955/6‑101 (MQ=255)


                                                                 gaaccc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGGCGGgggc                       >  1:1089849/5‑101 (MQ=255)


                                                                 gaaccc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcgc                       <  2:2145741/97‑1 (MQ=255)


                                                                  aaccc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                      <  1:1174397/98‑1 (MQ=255)


                                                                     cc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                   <  1:1327610/101‑1 (MQ=255)


                                                                      c.gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGa                  >  1:1949471/1‑101 (MQ=255)


                                                                        gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCCGGCGCcgcggc                 >  2:2243740/1‑99 (MQ=255)


                                                                        gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                 <  2:741093/101‑1 (MQ=255)


                                                                        gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                 <  2:1509941/101‑1 (MQ=255)


                                                                        gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                 <  1:2657458/101‑1 (MQ=255)


                                                                              ggcacggctggCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcg            <  2:2624383/91‑1 (MQ=255)


                                                                              ggcacggctggCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcg            <  2:1751087/91‑1 (MQ=255)


                                                                                 acggctggCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCgcgc         <  1:1124461/94‑1 (MQ=255)


                                                                                   ggctggCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg       <  2:2469764/96‑1 (MQ=255)


                                                                                   ggctggCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg       <  2:4954799/96‑1 (MQ=255)


                                                                                   ggctggCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg       <  2:802047/96‑1 (MQ=255)


                                                                                   ggctggCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCCGCTGGACGGGCCCCTGAGGCCGGGCGCCGCGAAGCCCACGCTg       >  1:4907567/6‑101 (MQ=255)


                                                                                    gcgggCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGa      <  1:440328/97‑1 (MQ=255)


                                                                                     ctggCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt     >  1:2711236/4‑101 (MQ=255)


                                                                                     ctggCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt     <  1:1298182/98‑1 (MQ=255)


                                                                                      tggCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc    <  1:250224/99‑1 (MQ=255)


                                                                                       ggCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg   <  2:1374052/100‑1 (MQ=255)


                                                                                        gCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGa  >  1:3449586/1‑101 (MQ=255)


                                                                                        gCCGA.GTTGCTCGCCCGGCAGGG..CG.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGa  <  2:900057/101‑1 (MQ=255)


GCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGA.A.CC.GCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGG..CG.C.G.GCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256117‑3256293

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG