Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,333,494	C→G	100%	intergenic (+40/‑126)	ccmH → / → paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,333,494	0	C→G	100.0%	143.2	39	intergenic (+40/‑126)	ccmH/paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  new base (0/39):  ref base (0/0):  total (0/39)

CGGTGCCGGTGCTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCCGCGCG  >  NC_014034/3333396‑3333591                                                                                                   |                                                                                                  cggtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCgcgggggg                                                                                                 <  1:1558842/101‑1 (MQ=255)   gtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                                                               <  2:2263534/101‑1 (MQ=255)   ggcccggtgcTCGCGGCCGAGGAAGAGGCGGGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCt                                                                                               <  1:3454226/98‑1 (MQ=255)    tgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTc                                                                                              <  2:199875/101‑1 (MQ=255)    tgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTc                                                                                              <  2:3273685/101‑1 (MQ=255)    ggccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTc                                                                                              <  1:1251141/100‑1 (MQ=255)     gccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                             <  1:1544125/101‑1 (MQ=255)     gccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                             <  1:498435/101‑1 (MQ=255)     gccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                             <  1:2462928/101‑1 (MQ=255)     gccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                             <  1:4885482/101‑1 (MQ=255)      ccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCg                                                                                            <  1:199377/101‑1 (MQ=255)       cggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGc                                                                                           <  2:4304576/101‑1 (MQ=255)          ggcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGGCCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccc                                                                                        <  2:3299143/100‑1 (MQ=255)           gcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccccc                                                                                       <  2:1944526/101‑1 (MQ=255)            ctctCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                                                      <  2:1628089/101‑1 (MQ=255)            ctctCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                                                      <  2:3187838/101‑1 (MQ=255)             tctcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCg                                                                                     <  2:2808794/101‑1 (MQ=255)              ctcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGc                                                                                    <  1:2748683/101‑1 (MQ=255)              ctcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGc                                                                                    <  2:2537781/101‑1 (MQ=255)                cGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAc                                                                                  <  1:3669979/101‑1 (MQ=255)                 ggCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcc                                                                                 <  1:484302/101‑1 (MQ=255)                  gCCGAGGAAGGGGCGCGGCGTAAAGACCGGCTGAAGGACTAGGGATTCCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAccc                                                                                <  1:1675678/101‑1 (MQ=37)                  gCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAccc                                                                                <  1:360256/101‑1 (MQ=255)                   ccGAGGGAAAGGCGCGGCGAAAAGCCCGGCTGAAGGACTGGGGATTTCGCCCGGGGCTGGCGCCCGGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccc                                                                               <  2:1746290/101‑1 (MQ=18)                         aaGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGc                                                                         <  1:3074187/101‑1 (MQ=255)                             ggCGCGGNGGAAANACNNGCTGAANGNNNANGGATTTCGCCCGGGGCTTGCGNCCCGGGGGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTAt                                                                     <  1:749311/101‑1 (MQ=25)                               cgcgGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTAttt                                                                   <  2:3582782/101‑1 (MQ=255)                                gcgGCTGAAAGACCTGCTGAAGGACGAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGCGGGCTCCGAACCCCGCACCCCCCGGACGTCtttt                                                                  <  2:153611/101‑1 (MQ=37)                                 cgGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTg                                                                 <  1:1930794/101‑1 (MQ=255)                                    cTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCa                                                              <  2:3285388/101‑1 (MQ=255)                                     tGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCaa                                                             <  2:4885302/101‑1 (MQ=255)                                     tGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCAGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCACCGGGCGTATTTTGCCaa                                                             <  1:4898316/101‑1 (MQ=255)                                      gAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAg                                                            <  1:763643/101‑1 (MQ=255)                                          gACCGGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGaaa                                                        <  2:4560113/101‑1 (MQ=255)                                                   gaGGACTGGGAATTTGACCCGGGGCTTGCGCCTCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGt                                               <  2:2753734/100‑1 (MQ=18)                                                    aGGCCTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTc                                              <  2:700537/101‑1 (MQ=255)                                                    aGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTc                                              <  2:4470252/101‑1 (MQ=255)                                                      gACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGCCCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTg                                            <  1:2021366/101‑1 (MQ=255)                                                                             tGCGCCCCGGGCGCCCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGCCGTGACGTTTGGTTTCGCttt                     <  1:2868270/101‑1 (MQ=255)                                                                                           cccGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGccc       <  2:292295/101‑1 (MQ=255)                                                                                                ggggggCTCCGCCCCCCGCACCCCCCGGGCGGATTTTGCCAAGGAAAAGCGGGGGGCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCTGCGCCcgcgcg  >  1:118393/1‑101 (MQ=25)                                                                                                ggggggCTCCGCCCCCCGCACCCCCCGGGCGGATTTTGCCAAGGAAAAGCGGGGGGCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCcgcgcg  >  1:4329378/1‑101 (MQ=255)                                                                                                   |                                                                                                  CGGTGCCGGTGCTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCCGCGCG  >  NC_014034/3333396‑3333591 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 37 ≤  ATCG