Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,604 T→C 100% A410A (GCT→GCC aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6040T→C100.0% 894.2 235A410A (GCT→GCCaceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (117/118):  ref base (0/0):  total (117/118)

Only 100 of 268 total aligned reads displayed.

CTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCG  >  NC_014034/3531506‑3531703
                                                                                                  |                                                                                                   
cTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTa                                                                                                   >  2:77613/1‑101 (MQ=255)
cTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTa                                                                                                   >  1:3121097/1‑101 (MQ=255)
cTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTa                                                                                                   >  2:1187872/1‑101 (MQ=255)
cTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTa                                                                                                   <  1:1582515/101‑1 (MQ=255)
 tGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTAt                                                                                                  <  2:4723913/101‑1 (MQ=255)
  gACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATc                                                                                                 >  2:2885824/1‑101 (MQ=255)
  gACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATc                                                                                                 >  2:1365985/1‑101 (MQ=255)
  gACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATc                                                                                                 >  1:4707710/1‑101 (MQ=255)
   aCCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATcc                                                                                                <  1:178372/101‑1 (MQ=255)
   aCCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATcc                                                                                                >  2:3772106/1‑101 (MQ=255)
   aCCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATcc                                                                                                <  2:3300510/101‑1 (MQ=255)
    ccTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCg                                                                                               >  2:4407203/1‑101 (MQ=255)
    ccTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCg                                                                                               <  1:3277771/101‑1 (MQ=255)
    ccTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCg                                                                                               <  1:4526399/101‑1 (MQ=255)
       acaacaACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAAc                                                                                            <  2:1222515/101‑1 (MQ=255)
       acaacaACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAAc                                                                                            <  2:2061204/101‑1 (MQ=255)
        caacaaCTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACg                                                                                           <  2:2923931/101‑1 (MQ=255)
         aacaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACgg                                                                                          >  2:4529982/1‑101 (MQ=255)
           caacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGc                                                                                        >  2:947622/1‑101 (MQ=255)
            aacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGcc                                                                                       >  1:1921084/1‑101 (MQ=255)
             acTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACNNNNNCCGAAGACGCCTATCCGAACGGGccc                                                                                      >  1:2590639/1‑101 (MQ=255)
             acTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGCGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGccc                                                                                      >  1:4992549/1‑101 (MQ=255)
              cTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCg                                                                                     <  1:2308915/101‑1 (MQ=255)
              cTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCg                                                                                     <  1:3941646/101‑1 (MQ=255)
                cGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGAAGCCTATCCGAACGGGCCCGAg                                                                                   >  2:3210938/1‑101 (MQ=255)
                 gCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGc                                                                                  >  2:2755021/1‑101 (MQ=255)
                   ccAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtg                                                                                <  1:4006071/101‑1 (MQ=255)
                     aGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgat                                                                              <  1:1461303/101‑1 (MQ=255)
                      gCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgatg                                                                             >  2:519200/1‑101 (MQ=255)
                        ttCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAGGACGCCTATCCGAACGGGCCCGGGCTGATGaa                                                                           >  1:2371693/1‑101 (MQ=255)
                        ttCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGaa                                                                           >  2:2338727/1‑101 (MQ=255)
                        ttCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGaa                                                                           <  1:3978841/101‑1 (MQ=255)
                         tCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAg                                                                          >  2:4647977/1‑101 (MQ=255)
                          cAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGc                                                                         <  1:2615662/101‑1 (MQ=255)
                            aCTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGccc                                                                       >  2:3246423/1‑101 (MQ=255)
                             cTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCg                                                                      <  2:101312/101‑1 (MQ=255)
                             cTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCg                                                                      <  1:2815961/101‑1 (MQ=255)
                             cTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCCGATGAAGCCCg                                                                      <  1:404290/101‑1 (MQ=255)
                              tGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGGCCgc                                                                     >  1:4852656/1‑100 (MQ=255)
                              tGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGa                                                                     >  1:11136/1‑101 (MQ=255)
                              tGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGa                                                                     <  2:3574642/101‑1 (MQ=255)
                                gACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACt                                                                   >  2:3921062/1‑101 (MQ=255)
                                 aCGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTa                                                                  >  1:2075008/1‑101 (MQ=255)
                                   gCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATg                                                                <  1:2601067/101‑1 (MQ=255)
                                   cttGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATg                                                                <  1:3715975/99‑1 (MQ=255)
                                    cTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATga                                                               <  2:4198650/101‑1 (MQ=255)
                                    cTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATga                                                               <  2:169026/101‑1 (MQ=255)
                                     tGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgag                                                              >  2:3715697/1‑100 (MQ=255)
                                     tGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgac                                                              >  1:3434778/1‑101 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                             >  2:2862342/1‑101 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                             <  1:3599154/101‑1 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                             >  2:239920/1‑101 (MQ=255)
                                         ccTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGaca                                                          >  1:629661/1‑101 (MQ=255)
                                         ccTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGaca                                                          >  2:1511736/1‑101 (MQ=255)
                                          cTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGacac                                                         >  2:1905260/1‑101 (MQ=255)
                                           tGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACg                                                        >  1:2176425/1‑101 (MQ=255)
                                            gcgcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGc                                                       <  1:4253489/101‑1 (MQ=255)
                                             cgcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcc                                                      >  2:1079877/1‑101 (MQ=255)
                                              gcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGccc                                                     >  2:701069/1‑101 (MQ=255)
                                               cAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccc                                                    >  1:1603052/1‑100 (MQ=255)
                                                aaGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccct                                                   <  1:3205524/101‑3 (MQ=255)
                                                aaGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccct                                                   <  1:1228668/101‑3 (MQ=255)
                                                aaGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccct                                                   <  2:3120859/101‑3 (MQ=255)
                                                 aGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                                  <  2:4070618/101‑1 (MQ=255)
                                                 aGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                                  <  1:3502401/101‑1 (MQ=255)
                                                  gCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGccactcg                                                 >  2:3620986/1‑96 (MQ=255)
                                                  gCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCg                                                 <  1:2039522/101‑1 (MQ=255)
                                                   cAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCgg                                                <  2:3482151/101‑1 (MQ=255)
                                                    aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGc                                               >  1:1135915/1‑101 (MQ=255)
                                                       tGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCcgc                                            <  2:4869192/101‑1 (MQ=255)
                                                           cgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAg                                        >  2:2555750/1‑101 (MQ=255)
                                                              gcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAATCCg                                     >  1:817213/1‑101 (MQ=255)
                                                              gcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCg                                     >  1:2043377/1‑101 (MQ=255)
                                                               cAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGa                                    >  1:928818/1‑101 (MQ=255)
                                                               cAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGa                                    >  2:719672/1‑101 (MQ=255)
                                                                 gTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATg                                  <  2:176808/101‑1 (MQ=255)
                                                                   ggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCATCGGCCGCGAACCCGAGGcc                                >  2:3374065/1‑100 (MQ=255)
                                                                     ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                              >  1:510799/1‑101 (MQ=255)
                                                                      tggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTgg                             >  2:1612884/1‑101 (MQ=255)
                                                                      tggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTgg                             >  1:197863/1‑101 (MQ=255)
                                                                       ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                            >  2:3852438/1‑101 (MQ=255)
                                                                       ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                            <  2:641636/101‑1 (MQ=255)
                                                                         ccGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTc                          <  1:3332108/101‑1 (MQ=255)
                                                                           gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                        >  1:4762246/1‑101 (MQ=255)
                                                                            aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCAGCGAAGCCGATGCCTGGCTCAAg                       <  1:4648300/101‑1 (MQ=255)
                                                                                cACGATCACCGAAGACGCCTATCCGAACGGGCCTGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCt                   <  1:4305589/101‑1 (MQ=255)
                                                                                 ctgATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATCAACCCCCACTATGACGACACGCCCCTCGGCCCCGAAGCCGATGCCTGGCTCAAAGCCtt                  <  2:4992219/99‑1 (MQ=255)
                                                                                 aCGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                  >  1:45981/1‑101 (MQ=255)
                                                                                   gATCACCGAAGCCCCCTATCCGAACGGGCCCGAGCTGATGAAGACCCACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                <  2:4178643/101‑1 (MQ=255)
                                                                                    acccccGAAGCCGCCTATCCGACCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa               <  2:4547778/97‑1 (MQ=255)
                                                                                      ccccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGa             <  1:1522885/99‑1 (MQ=255)
                                                                                      cACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGa             <  1:4833032/101‑1 (MQ=255)
                                                                                       cccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc            <  1:1376441/100‑1 (MQ=255)
                                                                                       aCCGAAGCCGCTTATCCGACCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc            <  1:1940974/101‑1 (MQ=255)
                                                                                         cGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCg          >  1:3846796/1‑101 (MQ=255)
                                                                                           aaGCCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCACTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag        <  1:3621257/101‑1 (MQ=255)
                                                                                            aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga       >  2:2176650/1‑101 (MQ=255)
                                                                                            aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga       <  2:2660224/101‑1 (MQ=255)
                                                                                              gcGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc     <  2:3938590/100‑1 (MQ=255)
                                                                                               cGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcg    >  2:4732748/1‑101 (MQ=255)
                                                                                                  |                                                                                                   
CTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCG  >  NC_014034/3531506‑3531703

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG