Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,596,459 C→G 100% G470G (GGC→GGG)  RCAP_rcc03391 → type I secretion system ATPase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,596,4590C→G100.0% 702.2 189G470G (GGC→GGG) RCAP_rcc03391type I secretion system ATPase
Reads supporting (aligned to +/- strand):  new base (105/84):  ref base (0/0):  total (105/84)

Only 100 of 214 total aligned reads displayed.

CCCGAGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGCCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAATCTCG  >  NC_014034/3596361‑3596556
                                                                                                  |                                                                                                 
cccgaGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcg                                                                                                 >  2:1737952/1‑101 (MQ=255)
   gagaTGATCGTCGATGCCGCGAAACGGGGGGCGTCGCATCAGCTGATCCTCGATCTGCGCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGct                                                                                              <  2:3780190/101‑1 (MQ=255)
    agaTGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGctc                                                                                             <  2:3517187/101‑1 (MQ=255)
     gatgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGctct                                                                                            <  1:2571268/101‑1 (MQ=255)
     gatgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGctcc                                                                                            >  2:4768301/1‑100 (MQ=255)
     gatgatCGTCGATGCCGCGAAACGGGCGGCAGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGgtct                                                                                            >  1:4499588/1‑101 (MQ=255)
      atgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCTCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCtctc                                                                                           <  2:2158366/101‑1 (MQ=255)
       tgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcg                                                                                          <  1:4737595/101‑1 (MQ=255)
       tgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcg                                                                                          >  1:2342053/1‑101 (MQ=255)
        gatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCCcgg                                                                                         >  1:4128629/1‑101 (MQ=255)
         atCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCTcggg                                                                                        >  1:1056099/1‑101 (MQ=255)
           cgtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcg                                                                                      >  1:418539/1‑101 (MQ=255)
            gtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcgg                                                                                     >  1:4883986/1‑101 (MQ=255)
            gtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcgg                                                                                     >  1:4434985/1‑101 (MQ=255)
              cgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCgggcgggc                                                                                   <  1:689753/101‑1 (MQ=255)
                aTGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAg                                                                                 <  1:1928508/101‑1 (MQ=255)
                aTGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAg                                                                                 >  2:2691721/1‑101 (MQ=255)
                aTGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAg                                                                                 <  1:3299371/101‑1 (MQ=255)
                aTGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCCGGGGGCTCTCGGGCGGGCaa                                                                                 >  2:2371695/1‑100 (MQ=255)
                    cgcgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATcc                                                                             <  2:4125109/101‑1 (MQ=255)
                    cgcgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATcc                                                                             <  2:1210265/101‑1 (MQ=255)
                    cgcgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCCCGGGCGGGCAGATcc                                                                             <  1:4259239/101‑1 (MQ=255)
                       gAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgc                                                                          >  2:352910/1‑101 (MQ=255)
                         aaCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgcgc                                                                        >  2:3152858/1‑101 (MQ=255)
                           cGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGGGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGGGGGCGGATTCAGCGCGt                                                                      >  1:1362704/1‑101 (MQ=255)
                           cGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGcgcgc                                                                      >  2:2166460/1‑100 (MQ=255)
                            gggCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCCCTCGGGCGGGCAGATCCAGCGCGTc                                                                     >  1:3906703/1‑101 (MQ=255)
                            gggCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTc                                                                     <  2:288105/101‑1 (MQ=255)
                            gggCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTc                                                                     >  2:2620499/1‑101 (MQ=255)
                             ggcggcggcGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCg                                                                    <  2:3091469/101‑1 (MQ=255)
                              gcggcggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGGTCTCGGGCGGGGAGATCCAGCGCGCCgg                                                                   >  2:740159/1‑101 (MQ=255)
                               cggcggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGc                                                                  >  1:2447370/1‑101 (MQ=255)
                                  cggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTg                                                               <  2:4763205/101‑1 (MQ=255)
                                   ggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTgg                                                              >  1:3711050/1‑101 (MQ=255)
                                     cgcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCCGCGCGTCGGCCCGGcc                                                            >  1:571682/1‑101 (MQ=255)
                                      gcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCTGGCGGGCAGATCCAGCGCGTCGGCCTGGccc                                                           <  1:2852211/101‑1 (MQ=255)
                                      gcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGccc                                                           <  1:3920524/101‑1 (MQ=255)
                                       cATCAGCTGAGCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCg                                                          <  1:1840934/101‑1 (MQ=255)
                                        aTCAGCTGATCCTCGATCTGCCCGAGGGATATGTCACGCGGGTTTCGCAGGCGGGCGCGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCgg                                                         <  1:4314807/101‑1 (MQ=255)
                                        aTCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCgg                                                         >  1:335515/1‑101 (MQ=255)
                                        aTCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCgg                                                         >  2:2643220/1‑101 (MQ=255)
                                            gCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGgcgc                                                     <  1:4592546/101‑1 (MQ=255)
                                              tGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCtt                                                   <  2:2982900/101‑1 (MQ=255)
                                               naTNCNNGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCttt                                                  <  1:2228562/100‑1 (MQ=255)
                                               gATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCttt                                                  >  1:364269/1‑101 (MQ=255)
                                               gATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCttt                                                  <  2:3328427/101‑1 (MQ=255)
                                               gATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCttt                                                  <  2:4898583/101‑1 (MQ=255)
                                                 tCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTAc                                                <  2:2460061/101‑1 (MQ=255)
                                                 tCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTAc                                                >  2:547069/1‑101 (MQ=255)
                                                 tCCTCGATCTGCCCGAGCGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTAc                                                <  2:4542140/101‑1 (MQ=255)
                                                     cGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCCGGCGCTTTACGGCa                                            >  2:294499/1‑101 (MQ=255)
                                                      gATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCTCGGGCGGGCAGATCCAGCGCGACGGCCTGGCCCGGGCGCTTTACGGCaa                                           >  1:3587081/1‑101 (MQ=255)
                                                       aTCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGGAAc                                          >  1:1461058/1‑101 (MQ=255)
                                                       aTCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAAc                                          <  1:5045422/101‑1 (MQ=255)
                                                         cTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAAccc                                        <  1:2312272/101‑1 (MQ=255)
                                                         cTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAAccc                                        >  1:2637335/1‑101 (MQ=255)
                                                         cTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAAccc                                        >  1:264469/1‑101 (MQ=255)
                                                           gCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCgg                                      <  2:4049677/101‑1 (MQ=255)
                                                           gCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGCGCGGGCATATCCAGCGCGTCGGCCTGGCCCCGGCGCTGTACCGCAACCCgg                                      >  2:1511035/1‑101 (MQ=255)
                                                             ccGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCgggg                                    >  1:1037418/1‑99 (MQ=255)
                                                              cGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgc                                   >  1:2908584/1‑101 (MQ=255)
                                                               gAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtggt                                  >  1:1451211/1‑99 (MQ=255)
                                                               gAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgct                                  <  2:619158/101‑1 (MQ=255)
                                                               gAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgct                                  <  1:2760990/101‑1 (MQ=255)
                                                               gAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgct                                  <  1:4523940/101‑1 (MQ=255)
                                                                aGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgctg                                 <  2:4260921/101‑1 (MQ=255)
                                                                 gggATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgctgc                                <  2:487790/101‑1 (MQ=255)
                                                                  ggATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTgctgct                               >  2:2668621/1‑101 (MQ=255)
                                                                   gATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGctgctg                              >  1:1328124/1‑101 (MQ=255)
                                                                     tatGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGt                            <  1:1435563/101‑1 (MQ=255)
                                                                     tatGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGt                            >  2:3678297/1‑101 (MQ=255)
                                                                     tatGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGt                            <  1:4949810/101‑1 (MQ=255)
                                                                      atGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGGGCTGCTgggg                           >  1:2505920/1‑99 (MQ=255)
                                                                       tGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCGCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGc                          <  1:2830504/101‑1 (MQ=255)
                                                                         acacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTc                        >  2:3351480/1‑101 (MQ=255)
                                                                         acacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTc                        >  1:1686587/1‑101 (MQ=255)
                                                                          cacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcg                       <  1:2468759/101‑1 (MQ=255)
                                                                             ccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgacg                    <  1:3589897/101‑1 (MQ=255)
                                                                             ccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgacg                    <  2:2541386/101‑1 (MQ=255)
                                                                             ccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgacg                    <  1:1617691/101‑1 (MQ=255)
                                                                             ccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgacg                    <  2:1287146/101‑1 (MQ=255)
                                                                             ccGGGTTTCGCAGGCGGGCGGGCGGCTCCCGGGGGGGGAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgacg                    >  2:2116494/1‑101 (MQ=255)
                                                                               gggTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAg                  <  2:4317755/101‑1 (MQ=255)
                                                                                 gTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGcc                <  1:916641/101‑1 (MQ=255)
                                                                                 gTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGcc                <  2:1383348/101‑1 (MQ=255)
                                                                                 gTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGcc                <  2:4223559/101‑1 (MQ=255)
                                                                                    tCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGaa             <  1:389638/101‑1 (MQ=255)
                                                                                    tCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGGGCTGCTGGGGCTCGACCAGCCGaa             >  1:964297/1‑101 (MQ=39)
                                                                                     cGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAAt            >  1:3035519/1‑101 (MQ=255)
                                                                                      gCAGGCGGGCGGGCGGCTCCCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATg           <  2:1196575/101‑1 (MQ=255)
                                                                                       cgggcgggcgGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGc          <  2:3625276/99‑1 (MQ=255)
                                                                                       cAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGc          >  2:1823938/1‑101 (MQ=255)
                                                                                       cAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGc          >  2:2227675/1‑101 (MQ=255)
                                                                                       cAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGc          <  2:4483787/101‑1 (MQ=255)
                                                                                       cAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGc          >  1:1802317/1‑101 (MQ=255)
                                                                                       cAGGCGGGAGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATgg          >  2:1892270/1‑100 (MQ=255)
                                                                                        aGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCg         >  2:1072400/1‑101 (MQ=255)
                                                                                        aGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCg         >  2:2444586/1‑101 (MQ=255)
                                                                                        aGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCg         >  2:2589288/1‑101 (MQ=255)
                                                                                             ggcgggCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAtct    >  1:3102396/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CCCGAGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGCCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAATCTCG  >  NC_014034/3596361‑3596556

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG