Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 367,286 G→A 100% A214T (GCC→ACC)  bztD → glutamate/aspartate ABC transporter ATP‑binding protein BztD

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034367,2860G→A100.0% 1121.2 307A214T (GCC→ACC) bztDglutamate/aspartate ABC transporter ATP‑binding protein BztD
Reads supporting (aligned to +/- strand):  new base (169/136):  ref base (0/1):  total (169/138)

Only 100 of 315 total aligned reads displayed.

TCGACGAACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCGCCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCG  >  NC_014034/367188‑367383
                                                                                                  |                                                                                                 
tCGACGAACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCAcc                                                                                                 <  1:566740/101‑1 (MQ=255)
 cgacgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCAccc                                                                                                <  1:1757526/101‑1 (MQ=255)
  gacgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCa                                                                                               >  1:3662939/1‑101 (MQ=255)
   acgaACCCCCCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCCTGACCATGCTCTGCGTCCCCCAt                                                                                              >  2:3071346/1‑101 (MQ=255)
   acgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCAt                                                                                              >  1:1785341/1‑101 (MQ=255)
    cgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATg                                                                                             >  1:520910/1‑101 (MQ=255)
    cgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATg                                                                                             <  1:2651629/101‑1 (MQ=255)
    cgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATg                                                                                             <  1:163113/101‑1 (MQ=255)
    cgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATg                                                                                             <  1:3902135/101‑1 (MQ=255)
     gaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATga                                                                                            >  1:3246923/1‑101 (MQ=255)
      aaCCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATgag                                                                                           >  2:68116/1‑101 (MQ=255)
      aaCCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATgag                                                                                           >  2:1471094/1‑101 (MQ=255)
         ccACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATg                                                                                        <  1:1461718/101‑1 (MQ=255)
           aCCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATggg                                                                                      >  1:126001/1‑101 (MQ=255)
            ccTCGGCGCTTGACCCCGAGATGATCAAGGGAGTGCTGGATCCGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGc                                                                                     <  1:2454863/101‑1 (MQ=255)
               cGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCttt                                                                                  <  1:3399018/101‑1 (MQ=255)
                ggCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTg                                                                                 <  1:4646911/101‑1 (MQ=255)
                   gcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGccc                                                                              <  1:1958337/101‑1 (MQ=255)
                   gcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGccc                                                                              >  2:3938037/1‑101 (MQ=255)
                   gcTTGACCCCGAGACGATCAAGGAAGTGCTGGAGACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGccc                                                                              <  1:4358702/101‑1 (MQ=255)
                     ttGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAg                                                                            >  2:886800/1‑101 (MQ=255)
                      tGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAgg                                                                           >  2:4825310/1‑101 (MQ=255)
                      tGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAgg                                                                           >  2:2325882/1‑101 (MQ=255)
                      tGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAgg                                                                           >  2:4578167/1‑101 (MQ=255)
                       gACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGc                                                                          >  1:983327/1‑101 (MQ=255)
                          cccGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGt                                                                       >  2:2594123/1‑101 (MQ=255)
                              agaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCg                                                                   <  1:4743701/101‑1 (MQ=255)
                              agaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCg                                                                   <  2:3248516/101‑1 (MQ=255)
                               gatgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGa                                                                  >  2:2289145/1‑101 (MQ=255)
                                atgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGaa                                                                 >  1:3621980/1‑101 (MQ=255)
                                atgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGGGGCGaa                                                                 >  1:3488661/1‑101 (MQ=255)
                                 tgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAc                                                                <  2:4057159/101‑1 (MQ=255)
                                   atCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCg                                                              <  2:3035326/101‑1 (MQ=255)
                                   atCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCg                                                              <  1:4392809/101‑1 (MQ=255)
                                   atCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCg                                                              <  1:3695519/101‑1 (MQ=255)
                                      aaGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGt                                                           >  2:3355274/1‑101 (MQ=255)
                                        ggAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGa                                                         >  2:4341742/1‑101 (MQ=255)
                                          aaGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATc                                                       >  1:4940738/1‑101 (MQ=255)
                                          aaGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATc                                                       <  1:1080221/101‑1 (MQ=255)
                                           aGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCt                                                      >  2:3190283/1‑101 (MQ=255)
                                              gCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCa                                                   <  2:2055279/101‑1 (MQ=255)
                                               cTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCAt                                                  <  1:3355537/101‑1 (MQ=255)
                                               cTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCAt                                                  <  2:4117442/101‑1 (MQ=255)
                                                 ggATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATgg                                                <  2:4504544/101‑1 (MQ=255)
                                                 ggATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATgg                                                >  2:4375924/1‑101 (MQ=255)
                                                 ggATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATgg                                                <  1:3003041/101‑1 (MQ=255)
                                                  gATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGc                                               >  2:3836291/1‑101 (MQ=255)
                                                  gATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGc                                               >  1:4864423/1‑101 (MQ=255)
                                                   aTACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGcc                                              <  2:604273/101‑1 (MQ=255)
                                                    tACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCg                                             <  1:3927866/101‑1 (MQ=255)
                                                    tACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATCAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCg                                             <  2:525307/101‑1 (MQ=255)
                                                      cGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGAc                                           >  1:789839/1‑101 (MQ=255)
                                                       gatgatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACg                                          >  2:845301/1‑101 (MQ=255)
                                                        atgatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACgg                                         <  2:487575/101‑1 (MQ=255)
                                                        atgatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACgg                                         >  2:198317/1‑101 (MQ=255)
                                                          gatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGc                                       <  1:1137431/101‑1 (MQ=255)
                                                           atCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCa                                      <  1:4509722/101‑1 (MQ=255)
                                                             ccAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGa                                    >  1:2125330/1‑101 (MQ=255)
                                                             ccAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGa                                    >  2:1210590/1‑101 (MQ=255)
                                                              cAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAt                                   <  1:68126/101‑1 (MQ=255)
                                                              cAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAt                                   >  2:3335929/1‑101 (MQ=255)
                                                              cAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAt                                   <  1:2716928/101‑1 (MQ=255)
                                                               aGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtc                                  <  2:2756892/101‑1 (MQ=255)
                                                               aGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtc                                  <  2:1121420/101‑1 (MQ=255)
                                                               aGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGCGATCTTCATGGCCGACGGGCAGAtc                                  >  2:1689264/1‑101 (MQ=255)
                                                                gCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcg                                 <  1:3819810/101‑1 (MQ=255)
                                                                 cTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgt                                >  2:2208329/1‑101 (MQ=255)
                                                                 cTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgt                                >  2:3562640/1‑101 (MQ=255)
                                                                    gCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGa                             >  2:1509209/1‑101 (MQ=255)
                                                                     ccGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAg                            >  1:2199978/1‑101 (MQ=255)
                                                                     ccGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAg                            <  2:4771286/101‑1 (MQ=255)
                                                                      cGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGc                           <  2:4940408/101‑1 (MQ=255)
                                                                        aGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAg                         >  2:214382/1‑101 (MQ=255)
                                                                        aGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAg                         <  2:3425577/101‑1 (MQ=255)
                                                                         ggAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGa                        <  1:856226/101‑1 (MQ=255)
                                                                          gAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaa                       <  1:3336192/101‑1 (MQ=255)
                                                                           aaGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaac                      <  1:3190534/101‑1 (MQ=255)
                                                                            aGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaaca                     <  1:1015578/101‑1 (MQ=255)
                                                                            aGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaaca                     >  2:1245874/1‑101 (MQ=255)
                                                                            aGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaaca                     >  2:346366/1‑101 (MQ=255)
                                                                              gCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaacaac                   >  2:1762123/1‑101 (MQ=255)
                                                                              gCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaacaac                   >  2:4159502/1‑101 (MQ=255)
                                                                               cATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAcc                  >  2:1365501/1‑101 (MQ=255)
                                                                               cATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAcc                  >  1:4114320/1‑101 (MQ=255)
                                                                                aTGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAccc                 <  2:1913127/101‑1 (MQ=255)
                                                                                aTGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAccc                 <  2:629699/101‑1 (MQ=255)
                                                                                  gACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGc               <  1:2326046/101‑1 (MQ=255)
                                                                                    cGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCAt             <  1:4437721/101‑1 (MQ=255)
                                                                                      aTGCTCTGCGTCGCCCATGACCGGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGa           <  2:2941828/101‑1 (MQ=255)
                                                                                      aTGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGa           <  1:2979037/101‑1 (MQ=255)
                                                                                      aTGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGa           >  2:3629489/1‑101 (MQ=255)
                                                                                         ctctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAGCCGGGTGATCTTCATGGCCGACTGGCAGATCGTCGAGCAGAACAACCCGCATGActt        >  1:4320101/1‑101 (MQ=255)
                                                                                         ctctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActt        >  1:92516/1‑101 (MQ=255)
                                                                                          tctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActtc       >  1:4226053/1‑101 (MQ=255)
                                                                                          tctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActtc       >  1:3122893/1‑101 (MQ=255)
                                                                                             gCGTCACCCATGAGATGGGCTGTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACttcttc    <  2:423400/101‑1 (MQ=255)
                                                                                              cGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcc   >  1:334099/1‑101 (MQ=255)
                                                                                              cGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcc   <  1:222929/101‑1 (MQ=255)
                                                                                              cGTCACCCATGAGATGGGCTTAGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcc   >  1:2861632/1‑101 (MQ=255)
                                                                                               gTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcca  >  1:201959/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
TCGACGAACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCGCCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCG  >  NC_014034/367188‑367383

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG