BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	432,428	G→T	2.1%	intergenic (+31/+117)	RCAP_rcc00403 → / ← RCAP_rcc00404	hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	432,428	0	G→T	2.1%	2.9 ‑2.9 ‑0.2	194	intergenic (+31/+117)	RCAP_rcc00403/RCAP_rcc00404	hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand): new base (3/1): ref base (48/142): total (51/143)
Fisher's exact test strand distribution p-value = 5.64e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.94e-01

Only 100 of 212 total aligned reads displayed.


ACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.T.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAA  >  NC_014034/432332‑432528


aCCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCgg                                                                                                    <  2:4596215/101‑1 (MQ=255)


aCCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCgg                                                                                                    <  2:4433535/101‑1 (MQ=255)


  ccGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGAc                                                                                                  <  2:2289827/101‑1 (MQ=255)


  ccGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGAc                                                                                                  <  2:195533/101‑1 (MQ=255)


       cgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTcccc                                                                                             >  1:1544049/1‑101 (MQ=255)


         cTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTcccccc                                                                                           <  2:2531365/101‑1 (MQ=255)


         cTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.CGCCGACTcccccc                                                                                           >  1:3302411/1‑101 (MQ=38)


         cTGGGCACGATGGGGACCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTcccccc                                                                                           <  2:401281/101‑1 (MQ=255)


          tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCg                                                                                          <  2:2419290/101‑1 (MQ=255)


           gggCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCgg                                                                                         <  2:3670682/101‑1 (MQ=255)


            ggCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCggg                                                                                        <  2:1367735/101‑1 (MQ=255)


              cACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGat                                                                                      <  1:570398/101‑1 (MQ=255)


              cACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGat                                                                                      <  1:1940700/101‑1 (MQ=255)


              cACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGat                                                                                      <  2:4092785/101‑1 (MQ=255)


              cACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGat                                                                                      <  1:1042097/101‑1 (MQ=255)


               aCGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGata                                                                                     <  2:1173834/101‑1 (MQ=255)


               aCGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGata                                                                                     <  1:3591930/101‑1 (MQ=255)


               aCGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGata                                                                                     <  1:2818534/101‑1 (MQ=255)


                cGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTccccccccgccaa                                                                                    >  1:114464/1‑94 (MQ=37)


                cGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGatat                                                                                    <  2:1532565/101‑1 (MQ=255)


                cGATGGGAATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGatat                                                                                    <  2:2586980/101‑1 (MQ=255)


                  aTGGGGATCGATCCGCGCACGCTTTCGGCGGGGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGGTGCCCCCC.G.TCC.TGCGGACTCCCCCCCGGATAttt                                                                                  >  1:4027497/1‑101 (MQ=25)


                  aTGGGGATCGATCCGCGCACGCTTTCGGCGGCGTCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATAttt                                                                                  <  1:4325687/101‑1 (MQ=255)


                  aTGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATAttt                                                                                  <  1:1152644/101‑1 (MQ=255)


                    ggggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCCGGATATTTGa                                                                                >  2:267588/1‑101 (MQ=255)


                     gggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCAAGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGG.C.C.TGCGGGCTCACCCCGGGGTCTTTGAg                                                                               >  2:4761560/1‑101 (MQ=25)


                      ggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAgg                                                                              <  1:3505025/101‑1 (MQ=255)


                      ggATCGATCCGCGCACGCTTTCGGCGGCGGCCGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGGGATTTGAgg                                                                              >  1:4514564/1‑101 (MQ=25)


                        atcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCa                                                                            <  1:226228/101‑1 (MQ=255)


                        atcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCa                                                                            >  2:3405162/1‑101 (MQ=255)


                         tcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCaa                                                                           <  1:4454060/101‑1 (MQ=255)


                           gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGa                                                                         <  2:3649754/101‑1 (MQ=255)


                            atcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGAt                                                                        <  2:387218/101‑1 (MQ=255)


                            atcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGAt                                                                        <  1:1642681/101‑1 (MQ=255)


                             tcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATg                                                                       <  2:2969983/101‑1 (MQ=255)


                              ccGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGa                                                                      <  2:2156034/101‑1 (MQ=255)


                                gcgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaaa                                                                    <  2:1745362/101‑1 (MQ=255)


                                gcgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaaa                                                                    <  2:4057177/101‑1 (MQ=255)


                                  gcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGAAATTTGAGGCAAGATGAAAGc                                                                  >  2:175555/1‑101 (MQ=255)


                                   cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                 <  2:826476/101‑1 (MQ=255)


                                     cGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGa                                                               <  2:128966/101‑1 (MQ=255)


                                       cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                             <  1:142804/101‑1 (MQ=255)


                                        tttCGGCGGGGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATcc                                                            <  1:4757869/101‑1 (MQ=255)


                                        tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GCC.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGTTcc                                                            <  1:3022955/101‑1 (MQ=21)


                                        tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATcc                                                            <  1:4621467/101‑1 (MQ=255)


                                             gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGcc                                                       >  1:368404/1‑100 (MQ=255)


                                              cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                      <  1:4086522/101‑1 (MQ=255)


                                                gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                    <  2:3441428/101‑1 (MQ=255)


                                                 gggcggGCCACGCCTGAGGCCGGGCAAGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                   <  1:4238478/100‑1 (MQ=255)


                                                   gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                 <  1:755558/101‑1 (MQ=255)


                                                    gtgtgcACGCCTGAGCGTGGGCCGGGGGCGCCTCCCCCCC.G.TCC.TGCGGACTCCCCCCGGGAGATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                <  2:4244139/96‑1 (MQ=16)


                                                    ggggCCACGCCTGAGGCGGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                <  1:1792129/100‑1 (MQ=255)


                                                      ggCCACGCCTGGGGCGGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                              <  2:1073898/101‑1 (MQ=21)


                                                      ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                              <  1:2017232/101‑1 (MQ=255)


                                                      ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                              <  2:1607290/101‑1 (MQ=255)


                                                      ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                              <  1:2346572/101‑1 (MQ=255)


                                                       gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                             <  2:1331622/101‑1 (MQ=255)


                                                        ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.t                                           <  2:686553/101‑1 (MQ=255)


                                                         cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                          <  1:867968/101‑1 (MQ=255)


                                                            gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGcc                                       <  1:3203470/101‑1 (MQ=255)


                                                              cTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGcccc                                     <  2:3240898/101‑1 (MQ=255)


                                                              cTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGcccc                                     >  1:2894562/1‑101 (MQ=255)


                                                               tGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCa                                    <  2:1086873/101‑1 (MQ=255)


                                                               tGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCa                                    <  1:925358/101‑1 (MQ=255)


                                                                gAGGCCGGGCAGGGGGGCGCTGGCCCCC.G.TCC.TGCGGACTCCCCCCGGGACATTTGAGGCAAGATGAAAGCCGATCCGGGCTTAGTTTCATG.TTGTCCCaa                                   >  2:4492459/1‑101 (MQ=18)


                                                                gAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTTGGGCAAGATGAAAAACGATACCGGCGTCGATTCATCTTTGCCCCa                                    >  2:4402018/1‑101 (MQ=11)


                                                                gAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaa                                   >  2:3227401/1‑101 (MQ=255)


                                                                 gggCCGGGCGGGGGGGGGCTGCCCCCC.T.C.C.CGCCGGCCCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAat                                 <  1:2749055/100‑1 (MQ=21)


                                                                 aGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                  <  2:50344/101‑1 (MQ=255)


                                                                 aGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                  <  2:3022136/101‑1 (MQ=255)


                                                                 aGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                  <  2:394358/101‑1 (MQ=255)


                                                                  ggCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAat                                 <  1:3858554/101‑1 (MQ=255)


                                                                   gCCGGGCAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                <  1:245528/101‑1 (MQ=255)


                                                                       ggacggggggCTCTGCCCCCC.G.G.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAtt                 >  2:2709821/5‑101 (MQ=2)


                                                                         cAGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCgg                          <  2:1019912/101‑1 (MQ=255)


                                                                          aGGGGGGCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGTTCCTTAAAACCAGATAAAAGGCCATACAGGCTTCGTTTCATC.TTGCCCCCAAAATCCCggg                         >  1:4135910/1‑101 (MQ=9)


                                                                            gggggCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCggggg                       >  2:3098637/1‑101 (MQ=255)


                                                                             ggggCGCTGCCCCCC.G.T.CGTGCGGACACCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCAGGGGt                      <  2:3379116/101‑1 (MQ=25)


                                                                              gggCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                     <  2:1553086/101‑1 (MQ=255)


                                                                              gggCGCTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                     <  2:3485013/101‑1 (MQ=255)


                                                                                gcgcTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGaa                   <  2:49757/101‑1 (MQ=255)


                                                                                gcgcTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGaa                   >  2:49535/1‑101 (MQ=255)


                                                                                 cgcTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAt                  >  2:3170758/1‑101 (MQ=255)


                                                                                 cgcTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAt                  <  2:4203244/101‑1 (MQ=255)


                                                                                 cgcTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAt                  <  1:2426842/101‑1 (MQ=255)


                                                                                  gcTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtt                 >  1:3449848/1‑101 (MQ=255)


                                                                                   cTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                <  1:1878669/101‑1 (MQ=255)


                                                                                   cTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                <  2:54148/101‑1 (MQ=255)


                                                                                   cTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                <  2:2753450/101‑1 (MQ=255)


                                                                                   cTGCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                >  1:4212722/1‑101 (MQ=255)


                                                                                     gCCCCCC.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTg              <  2:325722/101‑1 (MQ=255)


                                                                                      cccccc.G.TCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTgg             <  1:4837390/101‑1 (MQ=255)


                                                                                            ccgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCa         <  1:2609886/96‑1 (MQ=255)


                                                                                             cgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAg        <  1:3959461/97‑1 (MQ=255)


                                                                                              gtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc       >  1:3123025/4‑101 (MQ=255)


                                                                                              gtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc       <  1:1579785/98‑1 (MQ=255)


                                                                                                cc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa     <  1:1758668/100‑1 (MQ=255)


                                                                                                cc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCa     >  1:3162226/2‑101 (MQ=255)


                                                                                                 c.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaa    <  1:534096/101‑1 (MQ=255)


                                                                                                    gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGCATTTTGGCGCAGCCaaaa  >  2:4268636/1‑101 (MQ=255)


ACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.T.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAA  >  NC_014034/432332‑432528

Base quality scores:


ATCG

< 3 ≤


ATCG

< 16 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG