BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	539,743	T→G	100%	Y201D (TAC→GAC)	RCAP_rcc00505 →	type II secretion system protein E

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	539,743	0	T→G	100.0%	943.0	253	Y201D (TAC→GAC)	RCAP_rcc00505	type II secretion system protein E
Reads supporting (aligned to +/- strand): new base (143/110): ref base (0/0): total (143/110)

Only 100 of 270 total aligned reads displayed.


CGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCTACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACAA  >  NC_014034/539645‑539840


cGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGAc                                                                                                 <  2:149400/101‑1 (MQ=255)


cGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGAc                                                                                                 >  1:2953921/1‑101 (MQ=255)


 ggACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACg                                                                                                >  2:3152449/1‑101 (MQ=255)


  gACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGc                                                                                               >  1:2513396/1‑101 (MQ=255)


     gTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCg                                                                                            >  2:4828426/1‑101 (MQ=255)


        aCCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCt                                                                                         <  2:4774120/101‑1 (MQ=255)


        aCCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCt                                                                                         <  1:481186/101‑1 (MQ=255)


        aCCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCt                                                                                         <  1:3857190/101‑1 (MQ=255)


         ccTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTTGATGAAAGCAACCCCTATGTCGACGCCCGTCTg                                                                                        <  1:1819364/101‑1 (MQ=255)


         ccTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTg                                                                                        <  2:491679/101‑1 (MQ=255)


          cTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGGGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTgg                                                                                       >  1:590541/1‑101 (MQ=255)


            tCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGcc                                                                                     >  1:1463249/1‑101 (MQ=255)


            tCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGcc                                                                                     <  1:46175/101‑1 (MQ=255)


              aaGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGa                                                                                   >  2:1317287/1‑101 (MQ=255)


               aGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGAt                                                                                  >  1:446009/1‑101 (MQ=255)


                ggATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATg                                                                                 >  1:4418668/1‑101 (MQ=255)


                  aTGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGc                                                                               >  2:2911385/1‑101 (MQ=255)


                   tGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCt                                                                              >  2:4345590/1‑101 (MQ=255)


                   tGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCt                                                                              >  2:5029770/1‑101 (MQ=255)


                    gAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTc                                                                             <  2:3551662/101‑1 (MQ=255)


                    gAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTc                                                                             >  1:2392917/1‑101 (MQ=255)


                       aaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcgcg                                                                          <  2:4694587/101‑1 (MQ=255)


                          ccccTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGttt                                                                       <  2:225536/99‑1 (MQ=255)


                          cACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGttt                                                                       <  2:3764576/101‑1 (MQ=255)


                          cACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGttt                                                                       >  1:2020384/1‑101 (MQ=255)


                           aCCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGGGTTTc                                                                      >  1:2235260/1‑101 (MQ=255)


                           aCCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTc                                                                      <  1:3152700/101‑1 (MQ=255)


                           aCCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTc                                                                      <  1:1501564/101‑1 (MQ=255)


                            ccTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCa                                                                     <  2:1179693/101‑1 (MQ=255)


                            ccTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCa                                                                     <  1:3993805/101‑1 (MQ=255)


                                 tGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGGGTGTCGATGAAAGCAACCCCTATGTCTACGCCCGTCTGGCCGATGGCTCGCGTCTCAAcccg                                                                >  1:4142024/1‑101 (MQ=255)


                                  gCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGa                                                               >  1:3465123/1‑101 (MQ=255)


                                    ggATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATg                                                             <  1:2848910/101‑1 (MQ=255)


                                    ggATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATg                                                             <  1:1553727/101‑1 (MQ=255)


                                    ggATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATg                                                             >  2:3337117/1‑101 (MQ=255)


                                     gATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATgg                                                            <  1:1013937/101‑1 (MQ=255)


                                      atcatcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGt                                                           <  1:1317408/101‑1 (MQ=255)


                                      atcatcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGt                                                           >  1:2619513/1‑101 (MQ=255)


                                      atcagcGACAAGATCGTGTCCGCGGTGGGGCAGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGt                                                           <  2:670525/101‑1 (MQ=255)


                                         atcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGCGcc                                                        >  1:2314810/1‑101 (MQ=255)


                                          tcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGccc                                                       <  1:1487389/101‑1 (MQ=255)


                                           cgcaaaGCTCGTGTCCGCGGGGGGGCGGCGTGTCGATAAAAGCACCCCCTAGGCCGACCCCCGTCGGGCCGAGGGCTCGCGTTTAAACGCGATGGTGcccc                                                      <  2:4936147/97‑1 (MQ=255)


                                           cGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGcccc                                                      >  2:1306918/1‑101 (MQ=255)


                                             aCAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCg                                                    <  2:3014802/101‑1 (MQ=255)


                                              cAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCgg                                                   <  1:5026413/101‑1 (MQ=255)


                                              cAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCgg                                                   <  1:1394833/101‑1 (MQ=255)


                                               aaGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGt                                                  >  2:1406516/1‑101 (MQ=255)


                                               aaGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGt                                                  >  1:802576/1‑101 (MQ=255)


                                                aGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTc                                                 >  1:5042982/1‑101 (MQ=255)


                                                aGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTc                                                 <  2:2630356/101‑1 (MQ=255)


                                                aGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTc                                                 >  1:650968/1‑101 (MQ=255)


                                                aGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTc                                                 >  1:3892807/1‑101 (MQ=255)


                                                 gATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCCATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTcg                                                <  2:1672045/101‑1 (MQ=255)


                                                  aTCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTcgc                                               >  2:4544134/1‑101 (MQ=255)


                                                      tgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTg                                           >  1:3188121/1‑101 (MQ=255)


                                                      tgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTg                                           <  1:4940081/101‑1 (MQ=255)


                                                         ccGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGAt                                        <  2:3187870/101‑1 (MQ=255)


                                                           gcgGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATgg                                      <  2:16087/101‑1 (MQ=255)


                                                              gTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctc                                   <  1:4858098/101‑1 (MQ=255)


                                                                ggggCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgc                                 >  2:723280/1‑101 (MQ=255)


                                                                ggggCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgc                                 >  2:3686178/1‑101 (MQ=255)


                                                                  ggcggcGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgctc                               <  1:918977/101‑1 (MQ=255)


                                                                    cggcgTGACGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGt                             <  2:3388152/101‑1 (MQ=255)


                                                                      gcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGtct                           <  2:2825832/101‑1 (MQ=255)


                                                                       cgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGtctc                          >  2:4265087/1‑101 (MQ=255)


                                                                       cgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGtctc                          <  1:719742/101‑1 (MQ=255)


                                                                       cgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGtctc                          >  1:3588122/1‑101 (MQ=255)


                                                                        gtgtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCg                         <  1:3742221/101‑1 (MQ=255)


                                                                         tgtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGa                        >  2:1086569/1‑101 (MQ=255)


                                                                         tgtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGa                        >  1:3658650/1‑101 (MQ=255)


                                                                         tgtCGATGAAAGCAACCCCTATGTCGACGCCCGGCTGCCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGa                        <  1:4321916/101‑1 (MQ=255)


                                                                          gtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCNGGTCGCGGTGGATGGCTGCTTCGTCTCggg                       >  1:1932918/1‑99 (MQ=255)


                                                                           tCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATc                      <  1:2464528/101‑1 (MQ=255)


                                                                           tCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATc                      <  2:2787642/101‑1 (MQ=255)


                                                                            cGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATcc                     <  1:3351156/101‑1 (MQ=255)


                                                                             gATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCg                    >  1:4089077/1‑101 (MQ=255)


                                                                               tGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGa                  >  1:1135138/1‑101 (MQ=255)


                                                                                gcaaGGAAACCCTATGTCGACGCCCGTCCGGCCGATGGCTCGCGCTTCAACGCGAGGGTGCCCCCGGTCGCCGTGGGTGGCTCGCTCGTCTCGactcggga                 >  2:2436018/3‑94 (MQ=255)


                                                                                gAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGGGCCCCCGGTCGCGGGGGATGGCTCGCTCGTCTCGATCCGGaa                 >  1:3058581/1‑101 (MQ=255)


                                                                                gAAAGCAACCCCTAGGTCGACGCCCGTCTTGCCGATTGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGCCTCGCTCGTCTCGATCCGGaa                 <  2:4141707/101‑1 (MQ=255)


                                                                                  aaGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGCTCCGGAAAt               >  1:4206402/1‑101 (MQ=255)


                                                                                  aaGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAAt               <  1:3431708/101‑1 (MQ=255)


                                                                                   aGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAAtt              >  2:2024324/1‑101 (MQ=255)


                                                                                    gCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTc             >  1:4109431/1‑101 (MQ=255)


                                                                                     cAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCa            >  2:4026922/1‑101 (MQ=255)


                                                                                      aaCCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaa           <  2:4573388/101‑1 (MQ=255)


                                                                                      aaCCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaa           >  2:915827/1‑101 (MQ=255)


                                                                                        ccccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaaga         <  2:1279295/101‑1 (MQ=255)


                                                                                         cccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAATGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCCCGTCTCGATCCGGAAATTCaagaa        >  2:628659/1‑101 (MQ=255)


                                                                                          ccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaagaag       >  1:3232000/1‑101 (MQ=255)


                                                                                          ccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaagaag       <  1:2778223/101‑1 (MQ=255)


                                                                                           cTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAgg      <  2:1744226/101‑1 (MQ=255)


                                                                                           cTAGGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTTAGCGCGATGGTGCCCCGGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAgg      <  2:3858708/101‑1 (MQ=255)


                                                                                            tATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGa     >  1:1345840/1‑101 (MQ=255)


                                                                                            tATGTCGACGCCCGTCTGGCCGATGGCTCCCGTTTCAACGCGATGGGGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGa     >  2:2445514/1‑101 (MQ=255)


                                                                                              tGTCGACGCCCGTTTGGCCGATGGCTCGCGTTTCAACGCGATGCTGCCCCCGGTCGGGGTGGATGGCGCGCTCGGCTCGATCCGGAAATTCAAGAAGGACa   <  2:4170136/101‑1 (MQ=255)


                                                                                              tGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACa   >  2:2719202/1‑101 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTTAAGAAGGACaa  >  2:1986820/1‑101 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACaa  >  2:4393865/1‑101 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACaa  <  1:1079283/101‑1 (MQ=255)


CGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCTACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACAA  >  NC_014034/539645‑539840

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG