BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	580,960	G→A	2.0%	intergenic (+81/‑290)	RCAP_rcc00541 → / → RCAP_rcc00542	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	580,960	0	G→A	2.0%	2.3 ‑2.1 ‑0.1	197	intergenic (+81/‑290)	RCAP_rcc00541/RCAP_rcc00542	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (0/4): ref base (92/101): total (92/105)
Fisher's exact test strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.95e-01

Only 100 of 217 total aligned reads displayed.


GTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGG  >  NC_014034/580862‑581059


 tgtTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGgcg                                                                                                   <  1:3240439/101‑1 (MQ=255)


     ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                               <  2:4426866/101‑1 (MQ=255)


       gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                                                             <  1:4757738/101‑1 (MQ=255)


       gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                                                             <  1:3403107/101‑1 (MQ=255)


       gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                                                             <  2:1907239/101‑1 (MQ=255)


       gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                                                             <  1:4425145/101‑1 (MQ=255)


       gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                                                             <  2:2810510/101‑1 (MQ=255)


       cccTCGATTGAGGCGGCCCGGGCAGGGGGCGCTCCCCCCTCTTTCGGCTGCGCCGAAATTCC.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                                                             <  1:767079/100‑1 (MQ=25)


          tCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCC.CCCCCCGGGTATTTGAGGCAAGATAAACGGGCGCCTATCAcc                                                                                          >  1:2407286/1‑101 (MQ=25)


              ttGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGGGCCTATACCCTTgg                                                                                      >  2:3995544/1‑101 (MQ=21)


              ttGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTgg                                                                                      >  1:1531128/1‑101 (MQ=255)


               tGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCC.CCCCCGGGATATTTGAGGCCAGATAAACGGGCGCCTATCACCTTGGt                                                                                     >  1:2129768/1‑101 (MQ=25)


               tGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCCAAATTCA.CCCCCGGGATATTTGAGGGAAGATAAACGGGGGCCTATCACCTTGGt                                                                                     >  2:3806112/1‑101 (MQ=21)


                gAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTgggg                                                                                    >  2:539348/1‑99 (MQ=255)


                 aGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGc                                                                                   >  2:832280/1‑101 (MQ=255)


                  ggcggcCCGGGCAGGGGGGGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGGAACATAAACGGGCGCCTATTCCCTTTGGGCa                                                                                  >  2:2142073/1‑101 (MQ=18)


                  ggcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTTGGCTGCGCCGAAATTTA.CCCCCGGGATATTTTAGGCAAGATAAACGGGCGCCTATCACCTTGGTGcc                                                                                  >  1:4812793/1‑100 (MQ=21)


                  ggcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCa                                                                                  <  2:3787483/101‑1 (MQ=255)


                   gcggcCCGGGCAGGGGGGGCCGCCCACACTTTCGGCTGCGCCGGCATTCA.CCCCCGGGCGATTTGAGGGAAGATAAACAGGCGCCTATCACCCTCGTGcca                                                                                 >  2:3226163/1‑99 (MQ=255)


                    cggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCaaa                                                                                <  2:3736550/101‑1 (MQ=255)


                     ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAat                                                                               <  2:2709816/101‑1 (MQ=255)


                           ggCAGGGGGCGCTGCCCCCTCTTTCGGGTGGGCCGGAAATTA.CCCCCGGGGTATTTTAGGGAAGATAAACGGGCGCCCATCCCCCTGGGGCCAATATTCgg                                                                         >  2:352764/1‑101 (MQ=16)


                             cAGGGGGCGCTGCCCCCTCTTTTGGCTGCGCCGAAATTCA.CCCCCCGGGTATTTGAGGCAAGATAAAAGGGGGCCCATCACCCTGGTGCCAATAACCgggg                                                                       >  2:86290/1‑101 (MQ=17)


                             cAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgggg                                                                       >  2:4984320/1‑101 (MQ=255)


                              aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggggg                                                                      <  2:3596884/101‑1 (MQ=255)


                               gggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCAGCTTGGTGCAAATATCCgggggg                                                                     >  2:848348/1‑101 (MQ=255)


                                tccgatcTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGt                                                                    <  1:3655851/95‑1 (MQ=25)


                                ggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGGGCCTATCACCTTGGTGCAAATATCCggggggg                                                                    >  2:77383/1‑100 (MQ=255)


                                ggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGt                                                                    >  2:3038443/1‑101 (MQ=255)


                                  ggCGCTGCCCCCTCTTGCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGa                                                                  <  2:264896/101‑1 (MQ=255)


                                        gCCCCCTCTTTCGGCTGCGCCGAAATTCC.CCCCCGGGATATTTTAGGCAAGATAAACGGGCGCCTATCCCCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                                                            <  2:24419/101‑1 (MQ=255)


                                        gCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                                                            <  1:1900393/101‑1 (MQ=255)


                                        gCCCCCTCTTTCGGCCGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                                                            <  1:1199307/101‑1 (MQ=255)


                                          ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                          <  2:2863793/101‑1 (MQ=255)


                                          ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                          <  1:1879933/101‑1 (MQ=255)


                                          ccccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                          <  2:1063749/101‑1 (MQ=255)


                                           cccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                                         <  1:752232/101‑1 (MQ=255)


                                           cccTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGAGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAg                                                         >  1:2516206/1‑101 (MQ=255)


                                              tctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                      <  1:1774647/101‑1 (MQ=255)


                                               ctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGa                                                     >  2:3558872/1‑101 (MQ=255)


                                               ctTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGa                                                     >  1:1032956/1‑101 (MQ=255)


                                                tttCGGCTGCGCCGAAATTTA.CCCCCGCGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGGGAGGCCGAAAGCCGAg                                                    >  2:1323311/1‑101 (MQ=38)


                                                 ttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                                   >  2:3103399/1‑101 (MQ=255)


                                                 ttCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgg                                                   <  2:1766351/101‑1 (MQ=255)


                                                    ggCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAggggg                                                >  1:2091462/1‑101 (MQ=255)


                                                     gCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                               <  2:2509119/101‑1 (MQ=255)


                                                      cTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                              <  1:3363887/101‑1 (MQ=255)


                                                        ttcggccaaTTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTTTATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcg                                           <  1:4155632/94‑1 (MQ=37)


                                                        ttcggccaaTTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGGGGTTTATCTTGCCCCAAATATCCCGGGGGTGAGG.CG.AAGCCGAGGGGGCAgcg                                           <  2:205968/94‑1 (MQ=37)


                                                        gcgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgg                                            >  2:4835861/1‑100 (MQ=255)


                                                        gcgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgc                                            <  1:4463664/101‑1 (MQ=255)


                                                        gcgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGGGAGGCCGAAAGCCGAGGGGGCAgc                                            >  1:574855/1‑101 (MQ=255)


                                                         ggcCGAAATTCG.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                           <  1:3123790/100‑1 (MQ=255)


                                                         cgcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                           <  2:2977395/101‑1 (MQ=255)


                                                          gcCGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcgc                                          >  1:4378459/1‑101 (MQ=255)


                                                            cGAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccc                                        >  1:3876859/1‑101 (MQ=255)


                                                             gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                       >  2:1669061/1‑101 (MQ=255)


                                                             gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                       >  1:3064639/1‑101 (MQ=255)


                                                             gAAATTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                       >  2:2527134/1‑101 (MQ=255)


                                                               aaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                     >  1:2842200/1‑101 (MQ=255)


                                                               aaTTCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCt                                     <  1:518923/101‑1 (MQ=255)


                                                                 ttCG.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCttt                                   <  1:2457404/101‑1 (MQ=255)


                                                                 ttCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCGCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCttt                                   <  1:4917706/101‑1 (MQ=255)


                                                                  tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                  >  1:4764339/1‑101 (MQ=255)


                                                                  tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                  <  2:2378174/101‑1 (MQ=255)


                                                                  tCA.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCttta                                  >  1:851186/1‑100 (MQ=255)


                                                                   tcccccccGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                  <  2:405255/98‑1 (MQ=255)


                                                                    a.CCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                                >  2:1870794/1‑101 (MQ=255)


                                                                         ccGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtc                            <  1:4018968/101‑1 (MQ=255)


                                                                          cGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcg                           >  1:1981934/1‑101 (MQ=255)


                                                                              atatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGAAGCGCCCCCTTTTCCCGTCGtgtg                       >  2:2051313/1‑101 (MQ=255)


                                                                              atatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGCGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGCCGtgtg                       >  1:3953067/1‑101 (MQ=37)


                                                                               tatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGGCGTGTgg                      <  1:598176/101‑1 (MQ=255)


                                                                                atTTGAGGCAAGATAAACGGGCGCCTCTCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                     <  1:3038681/101‑1 (MQ=255)


                                                                                atTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGTAGCGCCCCCTTTTCCCGTCGTGTGGa                     >  1:4238994/1‑101 (MQ=255)


                                                                                atTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                     >  2:1162390/1‑101 (MQ=255)


                                                                                atTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                     >  1:3581139/1‑101 (MQ=255)


                                                                                  ttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                   <  2:2129620/101‑1 (MQ=255)


                                                                                   tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                  >  2:607729/1‑101 (MQ=255)


                                                                                   tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                  >  1:952556/1‑101 (MQ=255)


                                                                                   tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                  >  1:4711680/1‑101 (MQ=255)


                                                                                    gAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcgc                 >  2:327315/1‑101 (MQ=255)


                                                                                    gAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcgc                 <  1:2574058/101‑1 (MQ=255)


                                                                                       gCAAGATAAACGGGCGCCTATCACCTTGGTGCATCTACCCGGGGGGTGAGGCCGAAACCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCa              <  1:4320467/101‑1 (MQ=37)


                                                                                        cAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa             >  1:1725449/1‑101 (MQ=255)


                                                                                         aaGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAc            >  2:1818548/1‑101 (MQ=255)


                                                                                          agataaaCGGGTGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGCCAGCGCCCCCTTTTCCCGACGTGTGGATCGCGCAACg           >  2:3375130/6‑101 (MQ=38)


                                                                                            ataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGtt         >  2:2167091/4‑101 (MQ=255)


                                                                                             taaaCGGGCGCCTATCCCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc        <  1:2527315/99‑1 (MQ=255)


                                                                                             taaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc        >  2:2948921/3‑101 (MQ=255)


                                                                                             aaagcGGGCCCCTATCACCTTGGTGAAAATATCGGGGGGGTGAGGCCGAAAGCCGGGGGGGCAGCCCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc        <  1:2626506/97‑1 (MQ=255)


                                                                                              aaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGACGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa       >  2:2572410/2‑101 (MQ=255)


                                                                                               aaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAg      <  1:4611448/101‑1 (MQ=255)


                                                                                                aCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAgg     >  2:4559105/1‑101 (MQ=255)


                                                                                                 cGGGCGCCTATCACCTTGGTGCAAATATCGGGGGGGTGAGGCCGAAAGCGGAGGGGGGAGCGCTCCCTTTTCCCGTCGGGTGCATCGGGCAACGTTccgcc    >  2:3928035/1‑97 (MQ=255)


                                                                                                 cGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAggg    >  2:1484266/1‑100 (MQ=255)


                                                                                                  gggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCg   <  1:1692127/101‑1 (MQ=255)


                                                                                                  gggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCg   >  2:1605055/1‑101 (MQ=255)


                                                                                                   ggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCgg  <  2:4764016/101‑1 (MQ=255)


                                                                                                   ggCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCgg  >  1:4640802/1‑101 (MQ=255)


GTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCA.CCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGCGG  >  NC_014034/580862‑581059

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG