Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,753 C→G 6.2% A1618A (GCG→GCC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,7530C→G 6.2% 22.8 ‑1.4 ‑0.0208A1618A (GCG→GCC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (6/7):  ref base (124/71):  total (130/78)
Fisher's exact test strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 228 total aligned reads displayed.

TCTGCGCCGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCG  >  NC_014034/678653‑678853
                                                                                                    |                                                                                                    
  tGCGCCGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCcgc                                                                                                    <  1:4712661/101‑1 (MQ=255)
      ccGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGtt                                                                                                <  1:3060924/101‑1 (MQ=255)
       cGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGttt                                                                                               <  2:107466/101‑1 (MQ=255)
       cGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGttt                                                                                               <  2:1295905/101‑1 (MQ=255)
       cGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGttt                                                                                               >  2:2178585/1‑101 (MQ=255)
        gTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTc                                                                                              >  2:146205/1‑101 (MQ=255)
        gTGGTCACGGCCAGGATCCCCGCCGTGCTCGGGCCTGGCATGAGCGCCCTCCCGATCCCCGCCATGGCCGAGACCGAAACCTGGGCGATATCCGCGGTTTc                                                                                              <  2:726145/101‑1 (MQ=255)
         tGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCTATATCCGCGGTTTcc                                                                                             <  1:4258956/101‑1 (MQ=255)
         tGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTcc                                                                                             >  1:4959169/1‑101 (MQ=255)
          ggTCAGGGCCCGGATCGTAGCCGCGCTCAGTCTTGGCATCAGCCCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCa                                                                                            <  2:2951681/101‑1 (MQ=255)
           gTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAg                                                                                           <  2:3034483/101‑1 (MQ=255)
            tCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAgc                                                                                          >  1:257180/1‑101 (MQ=255)
            tCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAgc                                                                                          >  2:4149101/1‑101 (MQ=255)
            tCAGGGCGCGGATCTGCGCCGTGCTCAGGCGTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAgc                                                                                          <  2:1980436/101‑1 (MQ=255)
             cAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAgcg                                                                                         >  1:4882831/1‑101 (MQ=255)
             cAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAgcg                                                                                         <  2:375925/101‑1 (MQ=255)
                ggCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGcgccg                                                                                      >  2:4982559/1‑101 (MQ=255)
                ggCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGcgccg                                                                                      <  1:1953912/101‑1 (MQ=255)
                 gcgcGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGcgccgc                                                                                     <  1:4816617/101‑1 (MQ=255)
                  cgcgGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGTGATATCCGCGGTTTCCAGCGCcgcg                                                                                    <  1:582053/101‑1 (MQ=255)
                      gATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCt                                                                                <  1:1935153/101‑1 (MQ=255)
                      gATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCt                                                                                <  2:629191/101‑1 (MQ=255)
                      gATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCt                                                                                <  2:3524717/101‑1 (MQ=255)
                       aTCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTg                                                                               <  2:3172362/101‑1 (MQ=255)
                       aTCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTg                                                                               >  1:2363590/1‑101 (MQ=255)
                        tCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTgc                                                                              >  2:454199/1‑101 (MQ=255)
                        tCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTgc                                                                              <  1:368022/101‑1 (MQ=255)
                         cTGCGCCGTGGTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTgcg                                                                             >  1:4366679/1‑101 (MQ=255)
                            cgcCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCg                                                                          <  1:4559366/101‑1 (MQ=255)
                                gTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCt                                                                      >  2:4008177/1‑101 (MQ=255)
                                gTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCt                                                                      >  2:4380430/1‑101 (MQ=255)
                                 tGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTgcgagatcgga                                                                     >  2:1825214/1‑93 (MQ=255)
                                 tGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTc                                                                     >  2:2109747/1‑101 (MQ=255)
                                   cTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAg                                                                   >  1:1004151/1‑101 (MQ=255)
                                        gCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccg                                                              <  1:3995561/101‑1 (MQ=255)
                                         cTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgc                                                             >  2:1101326/1‑101 (MQ=255)
                                         cTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGAGCCGCGATCTGCGCCGTGCTCAGcgccgc                                                             >  2:4740848/1‑101 (MQ=255)
                                          ttGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCcgcg                                                            >  2:2416628/1‑101 (MQ=255)
                                           tGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGa                                                           >  1:3289169/1‑101 (MQ=255)
                                            ggCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGAt                                                          >  1:4973590/1‑101 (MQ=255)
                                             gCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATc                                                         >  2:3943361/1‑101 (MQ=255)
                                               aTCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTg                                                       >  2:1004791/1‑101 (MQ=255)
                                                tCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGc                                                      >  1:592229/1‑101 (MQ=255)
                                                 cAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCg                                                     >  1:226740/1‑101 (MQ=255)
                                                  aGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGt                                                    >  1:1123827/1‑101 (MQ=255)
                                                   gcgcCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTc                                                   >  1:4432270/1‑101 (MQ=255)
                                                     gcCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTcgc                                                 <  2:4020743/101‑1 (MQ=255)
                                                     gcCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTcgc                                                 >  1:2893193/1‑101 (MQ=255)
                                                      ccGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTcgcg                                                >  2:220611/1‑101 (MQ=255)
                                                       cGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCgcgc                                               >  1:3957290/1‑101 (MQ=255)
                                                         tCCCGCTCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTc                                             <  1:1018428/101‑1 (MQ=255)
                                                         tCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTc                                             <  1:2814440/101‑1 (MQ=255)
                                                             gATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGcc                                         >  2:4408458/1‑101 (MQ=255)
                                                             gATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGcc                                         >  2:3278896/1‑101 (MQ=255)
                                                             gATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGcc                                         >  2:1405623/1‑101 (MQ=255)
                                                              aTCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCg                                        >  1:3868824/1‑101 (MQ=255)
                                                                  ccGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgcc                                    <  2:813293/101‑1 (MQ=255)
                                                                    gCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccgc                                  >  1:3342939/1‑101 (MQ=255)
                                                                     ccATTGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccgcc                                 >  1:607837/1‑101 (MQ=255)
                                                                       aTGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTg                               >  2:3818772/1‑101 (MQ=255)
                                                                         ggCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgcg                             >  2:541434/1‑101 (MQ=255)
                                                                         ggCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgcg                             <  2:4910722/101‑1 (MQ=255)
                                                                         ggCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgcg                             >  1:3505347/1‑101 (MQ=255)
                                                                          gCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgcgc                            >  2:586183/1‑101 (MQ=255)
                                                                           ccGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGcc                           <  1:2183917/101‑1 (MQ=255)
                                                                            cGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCg                          >  2:677546/1‑101 (MQ=255)
                                                                              agaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTg                        >  1:4566957/1‑101 (MQ=255)
                                                                               gaCCGAAAGCTGGGCAATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTgg                       >  1:5000868/1‑101 (MQ=255)
                                                                                aCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTCAGCCGTggc                      >  2:1237982/1‑100 (MQ=255)
                                                                                 ccGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTc                     <  2:2363426/101‑1 (MQ=255)
                                                                                       gCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGcc               <  1:2968669/101‑1 (MQ=255)
                                                                                       gCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGcc               >  2:3652488/1‑101 (MQ=255)
                                                                                       gCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGcc               >  1:4293179/1‑101 (MQ=255)
                                                                                        cTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCa              >  2:278468/1‑101 (MQ=255)
                                                                                        cTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCa              >  2:4930940/1‑101 (MQ=255)
                                                                                         tGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCAc             >  2:1512839/1‑101 (MQ=255)
                                                                                            accagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGAt          >  1:2546915/6‑101 (MQ=2)
                                                                                             cGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATg         <  1:3819057/101‑1 (MQ=255)
                                                                                             cGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATg         <  2:1855715/101‑1 (MQ=255)
                                                                                              gATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGt        <  1:2439238/101‑1 (MQ=255)
                                                                                              gATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGt        >  2:1680322/1‑101 (MQ=255)
                                                                                               atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGGCGCCTGCGCCGTGGTCAGCGCCACGATGTc       >  1:343626/1‑101 (MQ=255)
                                                                                               atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTc       >  1:277263/1‑101 (MQ=255)
                                                                                               atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTc       <  2:3911133/101‑1 (MQ=255)
                                                                                               atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTc       >  1:1783208/1‑101 (MQ=255)
                                                                                               atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTc       >  1:1493783/1‑101 (MQ=255)
                                                                                               agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc       <  2:36773/99‑1 (MQ=2)
                                                                                                tatCCGCGGTTTCCAGCTCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCa      <  1:3272290/101‑1 (MQ=255)
                                                                                                gatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa      <  2:2969633/100‑1 (MQ=2)
                                                                                                gatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa      >  1:4624860/2‑101 (MQ=2)
                                                                                                 atCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCTTCAGCGCCACGATGTCAt     >  1:1906569/1‑101 (MQ=2)
                                                                                                 atCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt     <  2:3344740/101‑1 (MQ=2)
                                                                                                 atCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAt     >  1:1453423/1‑101 (MQ=255)
                                                                                                 atCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAt     >  1:2096005/1‑101 (MQ=255)
                                                                                                 atCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAt     <  1:1101440/101‑1 (MQ=255)
                                                                                                  tCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAtt    <  2:4603452/101‑1 (MQ=255)
                                                                                                   ccGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTc   >  1:1371075/1‑101 (MQ=255)
                                                                                                   ccGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTc   <  2:2365956/101‑1 (MQ=255)
                                                                                                   ccGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTc   <  1:535249/101‑1 (MQ=255)
                                                                                                    cgcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCg  <  2:2558929/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
TCTGCGCCGTGGTCAGGGCGCGGATCTGCGCCGTGCTCAGGCTTGGCATCAGCGCCGTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCG  >  NC_014034/678653‑678853

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG