Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,447 C→T 9.5% E720E (GAG→ACT)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4470C→T 9.5% 32.7 ‑4.1 ‑0.0168E720E (GAG→GAA) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (3/13):  ref base (79/73):  total (82/86)
Fisher's exact test strand distribution p-value = 1.61e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.03e-01

Only 100 of 189 total aligned reads displayed.

GCGTCGACAGCGCCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAA  >  NC_014034/681347‑681547
                                                                                                    |                                                                                                    
gcgTCGACAGCGCCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCgccgcc                                                                                                      <  2:4125793/101‑1 (MQ=255)
  gTCGACAGCGCCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTc                                                                                                    >  1:1100707/1‑101 (MQ=255)
  gTCGACAGCGCCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTc                                                                                                    >  2:2888385/1‑101 (MQ=255)
   acggcAGCGCCGCAATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCg                                                                                                   <  1:1519002/97‑1 (MQ=255)
     gACAGCGCCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAg                                                                                                 >  1:3982941/1‑101 (MQ=255)
     gACAGCGCCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAg                                                                                                 >  2:2352019/1‑101 (MQ=255)
        aGCGCCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAgcag                                                                                              >  1:3115245/1‑99 (MQ=255)
         gcgcCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGcgcc                                                                                             <  2:986524/101‑1 (MQ=255)
           gccgcGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGcgccgc                                                                                           <  2:1171606/101‑1 (MQ=255)
           ccctcgATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGcgccgc                                                                                           <  2:4501568/97‑1 (MQ=255)
            ccgcGATGTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCcgcg                                                                                          <  2:4850214/101‑1 (MQ=255)
             cgcgATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGa                                                                                         >  2:4309452/1‑101 (MQ=255)
                gATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCt                                                                                      <  2:2097249/101‑1 (MQ=255)
                 aTCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTg                                                                                     >  2:1129468/1‑101 (MQ=255)
                  tCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGc                                                                                    <  1:4519515/101‑1 (MQ=255)
                  tCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGc                                                                                    <  2:2445812/101‑1 (MQ=255)
                   cTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCg                                                                                   >  2:3050349/1‑101 (MQ=255)
                   cTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCg                                                                                   >  1:514676/1‑101 (MQ=255)
                   cTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCg                                                                                   >  2:3995371/1‑101 (MQ=255)
                    tGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGt                                                                                  <  1:1656132/101‑1 (MQ=255)
                     gcgcgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTg                                                                                 >  2:889777/1‑101 (MQ=255)
                     gcgcgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTg                                                                                 <  2:2013700/101‑1 (MQ=255)
                     gcgcgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTg                                                                                 >  2:2928914/1‑101 (MQ=255)
                     gcgcgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTg                                                                                 <  2:3141744/101‑1 (MQ=255)
                      cgcgcCTGAAAGCCCAGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGc                                                                                >  1:2396552/1‑101 (MQ=255)
                       tcgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCt                                                                               <  2:5041801/100‑1 (MQ=255)
                       gcgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCt                                                                               <  1:2843335/101‑1 (MQ=255)
                           cTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTc                                                                           <  2:2056033/101‑1 (MQ=255)
                           cTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTc                                                                           <  1:4390299/101‑1 (MQ=255)
                           cTGAAAGCCCGACCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTc                                                                           >  1:1955285/1‑101 (MQ=255)
                             gAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTTCGTGCTGGTCAg                                                                         >  1:2027285/1‑101 (MQ=255)
                             gAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAg                                                                         <  2:1845928/101‑1 (MQ=255)
                                aGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgcgc                                                                      >  2:4487618/1‑101 (MQ=255)
                                aGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgcgc                                                                      <  2:1368828/101‑1 (MQ=255)
                                aGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgcgc                                                                      <  2:3048653/101‑1 (MQ=255)
                                aGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCGCGAGCGCCGCGATCTGCGTGCTGGTCAgcgc                                                                      <  1:4481860/101‑1 (MQ=255)
                                    cGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCg                                                                  >  2:1532296/1‑101 (MQ=255)
                                      gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAt                                                                <  2:4790044/101‑1 (MQ=255)
                                      gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAt                                                                <  1:690547/101‑1 (MQ=255)
                                       ccATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATc                                                               <  2:4616803/101‑1 (MQ=255)
                                       ccATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATc                                                               <  1:1387547/101‑1 (MQ=255)
                                        cATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCt                                                              >  1:3875933/1‑101 (MQ=255)
                                         aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGCGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTg                                                             >  1:4751252/1‑101 (MQ=255)
                                         aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTg                                                             >  2:5014923/1‑101 (MQ=255)
                                          tCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgc                                                            <  2:2505273/101‑1 (MQ=255)
                                           cGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgcg                                                           >  2:3969248/1‑101 (MQ=255)
                                            gTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgcgc                                                          <  1:2085021/101‑1 (MQ=255)
                                             tggcttagcgcagcccGGAGAGCGCCGTCGCGCGGATCTACGTCAGATCTTCCGAATAGAGCGCCGCGTTCTGCGTGGTGGTCAGCGCGCCGATCTGCGcc                                                         <  2:2027141/95‑1 (MQ=19)
                                             tGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGcc                                                         <  1:4487938/101‑1 (MQ=255)
                                                 cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGc                                                     <  2:53838/101‑1 (MQ=255)
                                                  gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGcc                                                    <  1:547224/101‑1 (MQ=255)
                                                  gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGcc                                                    >  2:785457/1‑101 (MQ=255)
                                                  gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGcc                                                    <  2:2828017/101‑1 (MQ=255)
                                                          cccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGAcgcg                                            >  1:4137795/1‑101 (MQ=255)
                                                            cGGAGAGCTCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGcgcc                                          <  1:4246863/101‑1 (MQ=255)
                                                            cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGcgcc                                          <  1:4912142/101‑1 (MQ=255)
                                                             ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGcgccg                                         >  1:127957/1‑101 (MQ=255)
                                                               agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCgccgcc                                       >  2:3818583/1‑101 (MQ=255)
                                                               agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCgccgcc                                       <  1:1019758/101‑1 (MQ=255)
                                                                gagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCt                                      <  2:341714/101‑1 (MQ=255)
                                                                 agCGCCGTCGCGCTGATCTGCGCTAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTg                                     <  2:1829619/101‑1 (MQ=255)
                                                                   cgcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTgcg                                   >  1:1941853/1‑101 (MQ=255)
                                                                    gcCGTCGCGCTGATCTGCGCCAGATCCGCCGGCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTgcgc                                  >  1:4490819/1‑101 (MQ=255)
                                                                     ccGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGcc                                 <  1:4075698/101‑1 (MQ=255)
                                                                      cgccgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATGTGCGTGCTGGTCAGGGCGCCGATCTGCGCCGAGCCAAGACGCGCCGCCTGCGCcg                                <  2:619325/98‑1 (MQ=25)
                                                                       gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgt                               >  1:3677838/1‑101 (MQ=255)
                                                                       gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCATACGCcgt                               >  2:4007452/1‑101 (MQ=255)
                                                                        tCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtc                              <  1:2542191/101‑1 (MQ=255)
                                                                         cgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcg                             >  2:4795266/1‑101 (MQ=255)
                                                                         cgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcg                             >  2:2406248/1‑101 (MQ=255)
                                                                           cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                           >  1:4803815/1‑101 (MQ=255)
                                                                             cggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAg                         <  1:3216274/91‑1 (MQ=2)
                                                                             cTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAg                         >  2:3578378/1‑101 (MQ=255)
                                                                               gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                       >  2:4562526/1‑101 (MQ=255)
                                                                                  ccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCa                    <  1:5008976/96‑1 (MQ=2)
                                                                                  cTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCa                    <  1:3995673/101‑1 (MQ=255)
                                                                                    gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTGGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACg                  <  2:3281452/98‑1 (MQ=2)
                                                                                    gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACg                  <  2:2598648/98‑1 (MQ=2)
                                                                                     cgcCAGATCCGCCGCCTCGAGGGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGa                 >  1:3717894/1‑101 (MQ=255)
                                                                                     cgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGa                 >  1:794114/1‑101 (MQ=255)
                                                                                     cgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGa                 >  2:1629356/1‑101 (MQ=255)
                                                                                     caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGa                 <  2:1836725/99‑1 (MQ=9)
                                                                                      gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                <  1:18655/101‑1 (MQ=255)
                                                                                       ccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATg               <  2:2788658/101‑1 (MQ=9)
                                                                                       ccAGATCCGCCGGCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATg               >  1:2738498/1‑101 (MQ=255)
                                                                                       ccAGATCCGCCGCCTGGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATg               <  1:889882/101‑1 (MQ=255)
                                                                                       ccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATg               >  1:3937935/1‑101 (MQ=255)
                                                                                        cAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGt              <  1:3513890/101‑1 (MQ=255)
                                                                                           aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt           >  1:3870981/1‑101 (MQ=255)
                                                                                           aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGAAGCGCAGCCTGCGCCGTCGTCAGCGCCACGATGTCAt           >  2:2497699/1‑101 (MQ=255)
                                                                                             ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg         <  1:4181143/101‑1 (MQ=255)
                                                                                             ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg         <  1:3046268/101‑1 (MQ=255)
                                                                                             ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg         <  1:3280713/101‑1 (MQ=255)
                                                                                             ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg         >  1:4419831/1‑101 (MQ=255)
                                                                                               gccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCggg       >  2:3741002/1‑100 (MQ=255)
                                                                                                  gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc    <  2:4154348/101‑1 (MQ=255)
                                                                                                  gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc    >  2:173597/1‑101 (MQ=255)
                                                                                                    cTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaa  >  2:1854572/1‑101 (MQ=255)
                                                                                                    cTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaa  >  1:2907733/1‑101 (MQ=255)
                                                                                                    cTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTAGTCAGCGCCACGATGTCATCGGTGCCaa  <  1:1034975/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GCGTCGACAGCGCCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAA  >  NC_014034/681347‑681547

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG