BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	681,461	T→C	20.3%	M716V (ATC→GTC)	RCAP_rcc00630 ←	ice nucleation protein repeat family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	681,461	0	T→C	20.3%	105.9 ‑2.2 ‑0.2	202	M716V (ATC→GTC)	RCAP_rcc00630	ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand): new base (15/26): ref base (82/78): total (98/104)
Fisher's exact test strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.72e-01

Only 100 of 215 total aligned reads displayed.


GATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCC  >  NC_014034/681363‑681561


 aTCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTg                                                                                                     >  2:1129468/1‑101 (MQ=255)


  tCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGc                                                                                                    <  2:2445812/101‑1 (MQ=255)


    tGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGt                                                                                                  <  1:1656132/101‑1 (MQ=255)


     gcgcgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTg                                                                                                 >  2:2928914/1‑101 (MQ=255)


      cgcgcCTGAAAGCCCAGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGc                                                                                                >  1:2396552/1‑101 (MQ=255)


       gcgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCt                                                                                               <  1:2843335/101‑1 (MQ=255)


        cgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCta                                                                                              >  1:5042131/1‑100 (MQ=255)


           cTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTc                                                                                           <  2:2056033/101‑1 (MQ=255)


             gAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTTCGTGCTGGTCAg                                                                                         >  1:2027285/1‑101 (MQ=255)


              aaaGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgc                                                                                        >  2:472597/1‑101 (MQ=255)


               aaGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgcg                                                                                       >  1:4505818/1‑101 (MQ=255)


                aGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgcgc                                                                                      >  2:4487618/1‑101 (MQ=255)


                aGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgcgc                                                                                      <  2:3048653/101‑1 (MQ=255)


                aGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCGCGAGCGCCGCGATCTGCGTGCTGGTCAgcgc                                                                                      <  1:4481860/101‑1 (MQ=255)


                   ccGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGNGTGCTGGTCAGCGCGcc                                                                                   >  2:1838227/1‑101 (MQ=255)


                    cGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCg                                                                                  >  2:1532296/1‑101 (MQ=255)


                      gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.At                                                                               <  2:4790044/101‑1 (MQ=255)


                       ccATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATc                                                                              <  2:4616803/101‑1 (MQ=255)


                       ccATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATc                                                                              <  1:1387547/101‑1 (MQ=255)


                         aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTg                                                                            >  2:5014923/1‑101 (MQ=255)


                          tCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTgc                                                                           >  2:2026532/1‑101 (MQ=255)


                          tCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTgc                                                                           <  2:2505273/101‑1 (MQ=255)


                             ttgCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGTGCGCCGCGATCTGCCTGCTGGTGGGCGCGCCG.ATCTgcgcg                                                                        >  2:932109/3‑100 (MQ=38)


                             tGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGcc                                                                        <  1:4487938/101‑1 (MQ=255)


                              ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGGGCCG.ATCTGCGCCg                                                                       >  2:2361997/1‑101 (MQ=255)


                              ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCg                                                                       >  2:1797641/1‑101 (MQ=255)


                                ccGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAg                                                                     <  2:3735592/101‑1 (MQ=255)


                                 cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGc                                                                    <  1:3872949/101‑1 (MQ=255)


                                 cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGc                                                                    >  2:866258/1‑101 (MQ=255)


                                 cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGc                                                                    >  1:573455/1‑101 (MQ=255)


                                  gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGcc                                                                   >  2:785457/1‑101 (MQ=255)


                                    gcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCa                                                                 <  2:3982639/101‑1 (MQ=255)


                                     cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                <  1:519500/101‑1 (MQ=255)


                                     cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                <  1:871152/101‑1 (MQ=255)


                                     cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                <  2:4143183/101‑1 (MQ=255)


                                            cGGAGAGCTCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gcc                                                        <  1:4246863/101‑1 (MQ=255)


                                            cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gcc                                                        <  1:371856/101‑1 (MQ=255)


                                             ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gccg                                                       >  1:127957/1‑101 (MQ=255)


                                              gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gccgc                                                      >  1:4873147/1‑101 (MQ=255)


                                               agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.gccgcc                                                     >  2:4523938/1‑101 (MQ=255)


                                               agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.gccgcc                                                     <  1:1019758/101‑1 (MQ=255)


                                                   cgcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTgcg                                                 >  1:1941853/1‑101 (MQ=255)


                                                     ccGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGcc                                               <  1:4075698/101‑1 (MQ=255)


                                                      cgccgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATGTGCGTGCTGGTCAGGGCGCCG.ATCTGCGCCGAGCCAAGACGC.GCCGCCTGCGCcg                                              <  2:619325/98‑1 (MQ=25)


                                                      cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcg                                              <  2:1850689/101‑1 (MQ=255)


                                                       gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcgt                                             >  2:48974/1‑101 (MQ=255)


                                                       gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCATACGCcgt                                             >  2:4007452/1‑101 (MQ=255)


                                                        tCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.TCCGCCTGCGCcgtc                                            <  1:4995485/101‑1 (MQ=255)


                                                        gcgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcgtc                                            <  2:1452435/100‑1 (MQ=255)


                                                          gcgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcgtcgt                                          <  2:2917503/101‑1 (MQ=255)


                                                             cggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAg                                       <  1:3216274/91‑1 (MQ=2)


                                                              tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAgc                                      >  1:4154665/1‑101 (MQ=255)


                                                              ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAgc                                      <  1:1093843/92‑1 (MQ=2)


                                                                  cTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCa                                  <  1:3995673/101‑1 (MQ=255)


                                                                    gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                >  2:3791042/1‑101 (MQ=255)


                                                                    gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCTTGCTCGACAGGGCCAGA.AGCTGCGTCGAGCCCAG.CGCGGCCGCCTGCGCCGTCGTCAGCCCCACg                                >  2:1636690/4‑101 (MQ=2)


                                                                    gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTGGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                <  2:3281452/98‑1 (MQ=2)


                                                                    gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                <  2:2598648/98‑1 (MQ=2)


                                                                     cgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGa                               >  1:794114/1‑101 (MQ=255)


                                                                     caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGa                               <  2:1836725/99‑1 (MQ=9)


                                                                     caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGACGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGGGCCACGa                               >  2:5021035/3‑101 (MQ=9)


                                                                      gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGAt                              <  1:18655/101‑1 (MQ=255)


                                                                      accAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGAt                              <  1:3689095/100‑1 (MQ=2)


                                                                       ccAGATCCGCCGGCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATg                             >  1:2738498/1‑101 (MQ=255)


                                                                       ccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATg                             >  1:3937935/1‑101 (MQ=255)


                                                                        cAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGt                            <  1:3513890/101‑1 (MQ=255)


                                                                           aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                         <  2:3819267/101‑1 (MQ=255)


                                                                            tCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGATCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                        >  2:2825426/1‑101 (MQ=255)


                                                                             ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                       <  1:3046268/101‑1 (MQ=255)


                                                                             ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                       >  1:4419831/1‑101 (MQ=255)


                                                                             ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                       <  1:4181143/101‑1 (MQ=255)


                                                                               gcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                     >  2:550453/10‑101 (MQ=9)


                                                                                 ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                   <  1:4573722/94‑1 (MQ=9)


                                                                                  gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                  <  2:2089590/95‑1 (MQ=12)


                                                                                    ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                >  2:3423974/5‑99 (MQ=9)


                                                                                    ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                <  2:1078842/97‑3 (MQ=9)


                                                                                    cTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaa                >  1:2907733/1‑101 (MQ=255)


                                                                                     tCGAGCGCCGCGCTCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa               >  2:3519808/1‑101 (MQ=255)


                                                                                     tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa               <  1:1532426/101‑1 (MQ=255)


                                                                                      cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg              <  1:330870/101‑1 (MQ=255)


                                                                                      cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg              >  1:3856994/1‑101 (MQ=255)


                                                                                      cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg              >  2:4819878/1‑101 (MQ=255)


                                                                                       gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc             >  2:4187818/1‑101 (MQ=255)


                                                                                       gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc             <  1:2579101/101‑1 (MQ=255)


                                                                                       caGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc             <  2:1892595/100‑6 (MQ=9)


                                                                                        aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGCCATCGGTGCCAAAGcc            >  1:1315237/1‑101 (MQ=255)


                                                                                        aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc            <  1:201605/101‑1 (MQ=9)


                                                                                        aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc            >  2:3152601/1‑101 (MQ=9)


                                                                                        aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc            >  1:2113145/1‑101 (MQ=2)


                                                                                          cgccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct          >  2:2498584/1‑99 (MQ=2)


                                                                                           gccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt         <  2:1268038/101‑4 (MQ=9)


                                                                                            ccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGGCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg        <  2:394344/101‑5 (MQ=9)


                                                                                             cgcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGa       <  1:229757/101‑1 (MQ=255)


                                                                                              tcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt      <  2:3618756/100‑1 (MQ=255)


                                                                                              gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt      <  1:3969540/101‑1 (MQ=255)


                                                                                              gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt      <  1:4710408/101‑1 (MQ=255)


                                                                                               cgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAta     >  1:3619027/1‑100 (MQ=255)


                                                                                               cgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc     >  2:1597303/1‑101 (MQ=255)


                                                                                                gATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg    <  2:514597/101‑1 (MQ=255)


                                                                                                gATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg    <  2:3072643/101‑1 (MQ=255)


GATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCC  >  NC_014034/681363‑681561

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG