Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,473 T→A 20.8% T712S (ACC→TCC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4730T→A20.8% 119.5 ‑2.1 ‑0.3202T712S (ACC→TCC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (15/27):  ref base (81/79):  total (96/106)
Fisher's exact test strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.28e-01

Only 100 of 220 total aligned reads displayed.

CTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGC  >  NC_014034/681374‑681573
                                                                                                   |                                                                                                      
cTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTc                                                                                                       <  2:2056033/101‑1 (MQ=255)
cTGAAAGCCCGACCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTc                                                                                                       >  1:1955285/1‑101 (MQ=255)
   aaaGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgc                                                                                                    >  2:472597/1‑101 (MQ=255)
     aGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGGCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgcgc                                                                                                  >  2:272043/1‑101 (MQ=255)
        ccGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGNGTGCTGGTCAGCGCGcc                                                                                               >  2:1838227/1‑101 (MQ=255)
         cGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCg                                                                                              >  2:1532296/1‑101 (MQ=255)
           gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.At                                                                                           <  2:4790044/101‑1 (MQ=255)
            ccATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATc                                                                                          <  2:4616803/101‑1 (MQ=255)
              aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGCGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTg                                                                                        >  1:4751252/1‑101 (MQ=255)
              aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTg                                                                                        >  2:5014923/1‑101 (MQ=255)
               tCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTgc                                                                                       >  2:2026532/1‑101 (MQ=255)
                cGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGACCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTgcg                                                                                      <  2:2595383/101‑1 (MQ=255)
                   ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGGGCCG.ATCTGCGCCg                                                                                   >  2:2361997/1‑101 (MQ=255)
                   ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCg                                                                                   >  1:4486761/1‑101 (MQ=255)
                    gCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGa                                                                                  >  1:3513976/1‑101 (MQ=255)
                     ccGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAg                                                                                 <  2:3735592/101‑1 (MQ=255)
                      cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGc                                                                                >  2:866258/1‑101 (MQ=255)
                      cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGc                                                                                <  1:3872949/101‑1 (MQ=255)
                       gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGcc                                                                               <  1:547224/101‑1 (MQ=255)
                          cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                            <  1:871152/101‑1 (MQ=255)
                          cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                            >  1:3356525/1‑101 (MQ=255)
                          cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                            <  2:3015075/101‑1 (MQ=255)
                          cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                            <  2:4143183/101‑1 (MQ=255)
                           ccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGa                                                                           >  1:1621578/1‑101 (MQ=255)
                              gcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGAcgc                                                                        <  1:3949258/101‑1 (MQ=255)
                                 cGGAGAGCTCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gcc                                                                    <  1:4246863/101‑1 (MQ=255)
                                 cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gcc                                                                    <  1:4912142/101‑1 (MQ=255)
                                 cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gcc                                                                    <  1:371856/101‑1 (MQ=255)
                                 cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gcc                                                                    <  2:4839140/101‑1 (MQ=255)
                                  ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gccg                                                                   >  2:330817/1‑101 (MQ=255)
                                  ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGc.gccg                                                                   >  1:127957/1‑101 (MQ=255)
                                    agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.gccgcc                                                                 <  1:1019758/101‑1 (MQ=255)
                                         gcCGTCGCGCTGATCTGCGCCAGATCCGCCGGCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTgcgc                                                            >  1:4490819/1‑101 (MQ=255)
                                          ccGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGcc                                                           <  1:4075698/101‑1 (MQ=255)
                                           cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcg                                                          <  2:1850689/101‑1 (MQ=255)
                                           cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcg                                                          >  2:4075235/1‑101 (MQ=255)
                                            gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcgt                                                         >  1:3677838/1‑101 (MQ=255)
                                            gTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcgt                                                         >  2:48974/1‑101 (MQ=255)
                                             tCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcgtc                                                        <  1:2542191/101‑1 (MQ=255)
                                             gcgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcgtc                                                        <  2:1452435/100‑1 (MQ=255)
                                               gcgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCcgtcgt                                                      <  2:2917503/101‑1 (MQ=255)
                                                cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCgtcgtc                                                     >  1:4803815/1‑101 (MQ=255)
                                                  cggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAg                                                   <  1:3216274/91‑1 (MQ=2)
                                                   tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GGCGCCTGCGCCGTCGTCAgc                                                  >  1:2964804/1‑101 (MQ=255)
                                                   tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAgc                                                  >  1:4154665/1‑101 (MQ=255)
                                                   ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAgc                                                  <  1:1093843/92‑1 (MQ=2)
                                                    gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAgcg                                                 >  2:4562526/1‑101 (MQ=255)
                                                       ccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCa                                              <  1:5008976/96‑1 (MQ=2)
                                                       cTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCa                                              <  1:3995673/101‑1 (MQ=255)
                                                        cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCAc                                             <  1:901341/97‑1 (MQ=2)
                                                         gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                            >  2:3791042/1‑101 (MQ=255)
                                                         gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCTTGCTCGACAGGGCCAGA.AGCTGCGTCGAGCCCAG.CGCGGCCGCCTGCGCCGTCGTCAGCCCCACg                                            >  2:1636690/4‑101 (MQ=2)
                                                         gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTGGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                            <  2:3281452/98‑1 (MQ=2)
                                                         gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACg                                            <  1:3925425/98‑1 (MQ=2)
                                                          cgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGa                                           >  1:794114/1‑101 (MQ=255)
                                                          caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGa                                           <  2:1836725/99‑1 (MQ=9)
                                                          caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGACGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGGGCCACGa                                           >  2:5021035/3‑101 (MQ=9)
                                                           gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGAt                                          <  1:18655/101‑1 (MQ=255)
                                                               gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                      >  2:4499916/1‑101 (MQ=255)
                                                                aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                     <  2:3819267/101‑1 (MQ=255)
                                                                 tCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGATCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                                    >  2:2825426/1‑101 (MQ=255)
                                                                  ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                                   <  1:3280713/101‑1 (MQ=255)
                                                                  ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                                   <  1:4181143/101‑1 (MQ=255)
                                                                  ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                                   >  1:4419831/1‑101 (MQ=255)
                                                                   cgccgcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                  >  2:599939/1‑101 (MQ=255)
                                                                      ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                               <  1:4573722/94‑1 (MQ=9)
                                                                       gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                              <  2:2089590/95‑1 (MQ=12)
                                                                       gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                              >  2:173597/1‑101 (MQ=255)
                                                                        ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATCTCATCGGTGCCa                             <  1:2742357/101‑1 (MQ=255)
                                                                        ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATCTCATCGGTGCCa                             <  1:2732474/101‑1 (MQ=255)
                                                                         ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                            >  2:932608/5‑99 (MQ=9)
                                                                         ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                            >  2:3423974/5‑99 (MQ=9)
                                                                          tCGAGCGCCGCGCTCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                           >  2:3519808/1‑101 (MQ=255)
                                                                           cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                          <  2:3875641/101‑1 (MQ=255)
                                                                           cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                          >  1:3856994/1‑101 (MQ=255)
                                                                            gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                         >  2:4187818/1‑101 (MQ=255)
                                                                            gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                         <  1:2026676/101‑1 (MQ=255)
                                                                            caGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                         <  2:1892595/100‑6 (MQ=9)
                                                                             aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGCCATCGGTGCCAAAGcc                        >  1:1315237/1‑101 (MQ=255)
                                                                             aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                        >  2:3152601/1‑101 (MQ=9)
                                                                             aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                        >  1:2113145/1‑101 (MQ=2)
                                                                               cgccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                      >  2:2498584/1‑99 (MQ=2)
                                                                                gccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                     <  2:1268038/101‑4 (MQ=9)
                                                                                 ccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                    <  2:3644085/101‑5 (MQ=2)
                                                                                  cgcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGa                   <  1:229757/101‑1 (MQ=255)
                                                                                    cgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc                 >  2:1597303/1‑101 (MQ=255)
                                                                                     gATCTGCGTGCTGGTCGGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                <  2:794012/101‑1 (MQ=255)
                                                                                     gATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                <  2:514597/101‑1 (MQ=255)
                                                                                      accTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATcgc               >  2:3993603/3‑101 (MQ=2)
                                                                                       tCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc              <  2:1093129/101‑1 (MQ=255)
                                                                                       tCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcccc              <  1:2885131/101‑1 (MQ=255)
                                                                                        cTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg             >  2:762985/1‑101 (MQ=255)
                                                                                          gCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCgccgcc           >  2:3974770/1‑101 (MQ=255)
                                                                                              gCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc       >  1:2064511/1‑101 (MQ=255)
                                                                                               cTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc      <  1:1841773/101‑1 (MQ=255)
                                                                                               cTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAGCGCCGCCGTGcc      >  1:1380503/1‑101 (MQ=255)
                                                                                               cTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCAGATcggaagagcaca      >  1:4457354/1‑91 (MQ=25)
                                                                                                tGGTCCGCGCGCCG.ATCTGCGCCGGGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc     <  2:2417761/101‑1 (MQ=255)
                                                                                                tGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc     >  1:1529280/1‑101 (MQ=255)
                                                                                                   tCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc  <  1:1918060/101‑1 (MQ=255)
                                                                                                   |                                                                                                      
CTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGC.GCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGC  >  NC_014034/681374‑681573

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG