Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,546 A→G 8.9% F687F (TTT→TTC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,5460A→G 8.9% 32.4 ‑0.2 ‑1.1179F687F (TTT→TTC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (8/8):  ref base (92/71):  total (100/79)
Fisher's exact test strand distribution p-value = 7.93e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.94e-02

Only 100 of 205 total aligned reads displayed.

CCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCC  >  NC_014034/681446‑681646
                                                                                                     |                                                                                                    
ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCa                                                                                                      <  1:2335056/101‑1 (MQ=255)
ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATCTCATCGGTGCCa                                                                                                      <  1:2732474/101‑1 (MQ=255)
 cTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaa                                                                                                     >  1:2907733/1‑101 (MQ=255)
 cTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTAGTCAGCGCCACGATGTCATCGGTGCCaa                                                                                                     <  1:1034975/101‑1 (MQ=255)
  tCGAGCGCCGCGCTCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                                                                                    >  2:3519808/1‑101 (MQ=255)
  tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                                                                                    <  1:1532426/101‑1 (MQ=255)
   cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                                                                                   >  1:3856994/1‑101 (MQ=255)
   cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                                                                                   <  2:4749914/101‑1 (MQ=255)
    gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                                                                                                  <  1:2579101/101‑1 (MQ=255)
     aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGCCATCGGTGCCAAAGcc                                                                                                 >  1:1315237/1‑101 (MQ=255)
     aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                                                                                 <  2:4721859/101‑1 (MQ=9)
     aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                                                                                 <  1:201605/101‑1 (MQ=9)
     aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                                                                                 >  1:2113145/1‑101 (MQ=2)
       cgccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                                                                                               >  1:4160371/1‑99 (MQ=2)
       cgccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                                                                                               <  2:1690231/101‑3 (MQ=9)
        gccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                                                                                              <  2:1268038/101‑4 (MQ=9)
         ccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGGCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                                                                                             <  2:394344/101‑5 (MQ=9)
           tcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                                                                                           <  2:3618756/100‑1 (MQ=255)
           gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                                                                                           <  1:3971375/101‑1 (MQ=255)
           gcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                                                                                           <  1:4710408/101‑1 (MQ=255)
            cgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc                                                                                          >  2:1597303/1‑101 (MQ=255)
             gATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                                                                         <  2:3072643/101‑1 (MQ=255)
              accTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATcgc                                                                                        >  2:3993603/3‑101 (MQ=2)
                cTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                                                                                      >  2:1405893/1‑101 (MQ=255)
                cTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                                                                                      <  2:4872820/101‑1 (MQ=255)
                       cTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc                                                                               <  1:1841773/101‑1 (MQ=255)
                       cTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAGCGCCGCCGTGcc                                                                               >  1:1380503/1‑101 (MQ=255)
                        tGGTCCGCGCGCCG.ATCTGCGCCGGGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc                                                                              <  2:2417761/101‑1 (MQ=255)
                         tgTCAGCGCGCCG.ATCTGCGCCGACCCCAGACGCGCCGCCTGGGCCGTCGGCAGCGCCACGATGTCATCGGGGCCAAAGCCGCGGATCGCCGCCGTGCCCa                                                                             <  2:3304852/100‑1 (MQ=37)
                           tCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc                                                                           <  1:1918060/101‑1 (MQ=255)
                            cAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCaacc                                                                          >  2:5039061/1‑98 (MQ=255)
                            cAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg                                                                          >  2:3677434/1‑101 (MQ=255)
                            cAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg                                                                          <  2:4422640/101‑1 (MQ=255)
                             cgcgcgcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc                                                                         <  1:2362161/100‑1 (MQ=255)
                              gcgcgcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgcc                                                                        <  1:785559/101‑1 (MQ=255)
                               cgcgcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgccg                                                                       <  2:3803883/101‑1 (MQ=255)
                                gcgcCG.ATCTGCGCCGAGCCCGGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgccgc                                                                      >  1:2862564/1‑101 (MQ=255)
                                  gcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCa                                                                    >  2:3324296/1‑101 (MQ=255)
                                   ccG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAc                                                                   >  1:365400/1‑101 (MQ=255)
                                     g.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg                                                                 >  1:2059540/1‑101 (MQ=255)
                                      gagcTGGGCCGAGGCCAGACGCCCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAACCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg                                                                 <  2:2964576/98‑1 (MQ=25)
                                       aTCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGt                                                                >  1:1812013/1‑101 (MQ=255)
                                          tGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCCCCGTCGc                                                             >  1:2002993/1‑101 (MQ=255)
                                             gcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGa                                                          <  2:3870689/101‑1 (MQ=255)
                                                gAGCCCAGACGCGCCGCCTGCGCCTTCGTCAGCGCCCCGATGTCATCGGTGCCCAAGCCGCGGATCCCCGCCCTGCCCCGCGCCCCCACCCTCGCCGAGGc                                                       >  1:4867193/1‑101 (MQ=37)
                                                gAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGc                                                       >  1:2763104/1‑101 (MQ=255)
                                                gAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGc                                                       >  1:1537200/1‑101 (MQ=255)
                                                 aGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGTCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGcc                                                      <  1:4304949/101‑1 (MQ=255)
                                                 aGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGcc                                                      <  2:670230/101‑1 (MQ=255)
                                                   cccAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGTATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAg                                                    <  2:3326879/101‑1 (MQ=255)
                                                    ccAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgc                                                   <  2:573376/101‑1 (MQ=255)
                                                      aGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCAGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcgc                                                 >  2:771215/1‑101 (MQ=255)
                                                       gACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgcc                                                >  1:4064672/1‑101 (MQ=255)
                                                          gcgcCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCCCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCcgcg                                             <  1:4007754/101‑1 (MQ=255)
                                                           cgccgcCTGCGCCGTCGTCAGTGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgcaa                                            >  1:3723404/1‑99 (MQ=25)
                                                            gccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgcaag                                           >  1:1932727/1‑98 (MQ=25)
                                                            gccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGAt                                           <  1:3578648/101‑1 (MQ=255)
                                                            gcccccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgcaag                                           <  1:2498758/101‑4 (MQ=25)
                                                             ccgccTGCGCCGTCGTCAGCGCCGCGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATc                                          <  2:4490499/101‑1 (MQ=255)
                                                             ccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATc                                          <  1:2825639/101‑1 (MQ=255)
                                                               gccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTg                                        >  2:2539822/1‑101 (MQ=37)
                                                                ccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgc                                       >  2:1751623/1‑101 (MQ=255)
                                                                   gcgcCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGcc                                    >  2:28733/1‑101 (MQ=255)
                                                                   gcgcCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGcc                                    >  2:2332786/1‑101 (MQ=255)
                                                                     gcCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGGCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGt                                  >  1:4271667/1‑101 (MQ=255)
                                                                      ccGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTg                                 >  2:4142264/1‑101 (MQ=255)
                                                                      ccGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTg                                 >  1:1952956/1‑101 (MQ=255)
                                                                       cgtcgtCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGc                                <  2:1946934/101‑1 (MQ=255)
                                                                         tcgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTc                              <  1:1511117/101‑1 (MQ=255)
                                                                          cgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCa                             >  1:967349/1‑101 (MQ=255)
                                                                            tcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgc                           >  2:4622481/1‑101 (MQ=255)
                                                                             cAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                          >  1:1469820/1‑101 (MQ=255)
                                                                             cAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcc                          >  2:3192508/1‑100 (MQ=255)
                                                                               gcgcCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgcc                        <  2:3717616/101‑1 (MQ=255)
                                                                                cgcCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccg                       <  1:3324559/101‑1 (MQ=255)
                                                                                caccACGATGTCATCGGTGCCAAAGCCGCGTATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCGGCGCCGTGCTCAGcgccg                       <  2:3856709/99‑1 (MQ=255)
                                                                                  ccACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgcc                     <  2:2907520/101‑1 (MQ=255)
                                                                                     cGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCgccgccgcc                  >  2:4985112/1‑101 (MQ=255)
                                                                                     cGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCgccgccgcc                  >  1:1412333/1‑101 (MQ=255)
                                                                                      gATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCt                 >  2:3265453/1‑101 (MQ=255)
                                                                                      gATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCt                 >  2:4312867/1‑101 (MQ=255)
                                                                                       aTGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTg                <  2:967244/101‑1 (MQ=255)
                                                                                       aTGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTg                >  1:1862791/1‑101 (MQ=255)
                                                                                       aTGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTg                >  2:1593751/1‑101 (MQ=255)
                                                                                       aTGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTg                >  2:1075359/1‑101 (MQ=255)
                                                                                         gTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCa              >  2:3395735/1‑101 (MQ=255)
                                                                                          tCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAc             >  2:1243477/1‑101 (MQ=255)
                                                                                          tCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAc             >  1:2413334/1‑101 (MQ=255)
                                                                                          tCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAc             <  1:600018/101‑1 (MQ=255)
                                                                                          tCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAc             >  1:2719592/1‑101 (MQ=255)
                                                                                          tCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAc             >  1:4451349/1‑101 (MQ=255)
                                                                                            aTCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCg           >  1:1688751/1‑101 (MQ=255)
                                                                                            aTCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCg           <  1:3050587/101‑1 (MQ=255)
                                                                                            aTCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCg           <  1:4524258/101‑1 (MQ=255)
                                                                                                gTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGcc       >  1:950041/1‑101 (MQ=255)
                                                                                                 tGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCa      <  1:1259911/101‑1 (MQ=255)
                                                                                                    cAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGc   <  2:1931445/101‑1 (MQ=255)
                                                                                                    cAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGc   >  2:851872/1‑101 (MQ=255)
                                                                                                     aaaGCCGCGGATCGCCGCCGTGCCCAGCTCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc  >  1:4574784/1‑101 (MQ=255)
                                                                                                     aaaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc  >  2:2081491/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
CCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCC  >  NC_014034/681446‑681646

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG