Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	797,964	G→C	100%	G183G (GGG→GGC)	sucB →	dihydrolipoyllysine‑residue succinyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	797,964	0	G→C	100.0%	173.6	47	G183G (GGG→GGC)	sucB	dihydrolipoyllysine‑residue succinyltransferase
Reads supporting (aligned to +/- strand):  new base (3/44):  ref base (0/0):  total (3/44)

CGAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGGTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGC.GAAGGATGTCGAACATGCCCCCGCGGCGA  >  NC_014034/797865‑798058                                                                                                    |                                                                                                cGAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGGGGGCGTCTGGCGATCCTCGGGGGggggg                                                                                                >  1:4236684/1‑100 (MQ=255)  gAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGTGCGGTCGTCTGGCGATCCTGGCGGCGGGCTc                                                                                               <  1:4481968/101‑1 (MQ=255)  gAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTc                                                                                               <  1:2379167/101‑1 (MQ=255)   cggtgtcGGCCCGGGCGGCGGGCGTGCTGGGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCt                                                                                              <  1:2315498/95‑1 (MQ=255)   aGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCt                                                                                              <  1:2923992/101‑1 (MQ=255)      ttCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGcc                                                                                           <  2:4989352/101‑1 (MQ=255)      ttCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGcc                                                                                           <  1:2127554/101‑1 (MQ=255)       tCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCcg                                                                                          <  2:2080427/101‑1 (MQ=255)       tCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCcg                                                                                          <  2:1111451/101‑1 (MQ=255)        ccGGCCCCGGCGGCGGGCGTGCTGAGCGAGACCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCcgc                                                                                         <  1:1021334/101‑1 (MQ=255)          ggCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTTCCGCgg                                                                                       >  1:3255127/1‑101 (MQ=255)              ccGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTcc                                                                                   <  2:637226/101‑1 (MQ=255)              ccGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTcc                                                                                   <  2:1346973/101‑1 (MQ=255)              ccGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTcc                                                                                   <  1:951155/101‑1 (MQ=255)               cggcggcggGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCa                                                                                  <  2:3454756/101‑1 (MQ=255)               cggcggcggGCGGGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCa                                                                                  <  1:3033710/101‑1 (MQ=255)                  cggcggGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGcc                                                                               <  2:3028841/101‑1 (MQ=255)                   ggcgggcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGccc                                                                              <  2:2276032/101‑1 (MQ=255)                     cgggcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCg                                                                            <  2:3019275/101‑1 (MQ=255)                      gggcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCgg                                                                           <  2:3720896/101‑1 (MQ=255)                        gcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGcc                                                                         <  2:2950541/101‑1 (MQ=255)                        gcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGcc                                                                         <  1:1890440/101‑1 (MQ=255)                         cgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCg                                                                        <  1:393356/101‑1 (MQ=255)                           tGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCt                                                                      <  1:432143/101‑1 (MQ=255)                             cTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTgc                                                                    <  1:4433027/101‑1 (MQ=255)                               gAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTgcgg                                                                  <  2:2399694/101‑1 (MQ=255)                                aGCGAGATCCTCGCCCCTGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTgcggc                                                                 <  2:4540419/101‑1 (MQ=255)                                aGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTgcggc                                                                 <  2:4228857/101‑1 (MQ=255)                                    ggaTCCTCGCCCCGGTGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGTCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGgcc                                                             <  2:471631/100‑1 (MQ=255)                                    agaTCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGgcc                                                             <  2:3312371/101‑1 (MQ=255)                                      aTCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGgccgc                                                           <  1:1403890/101‑1 (MQ=255)                                        ccTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGccc                                                         <  1:1888019/101‑1 (MQ=255)                                         cTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGcccc                                                        <  1:3968752/101‑1 (MQ=255)                                         cTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGcccc                                                        <  1:2052814/101‑1 (MQ=255)                                          tCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGccccc                                                       <  2:4904508/101‑1 (MQ=255)                                          tCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGccccc                                                       <  1:1362495/101‑1 (MQ=255)                                           cGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCcg                                                      <  1:1753221/101‑1 (MQ=255)                                           cGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCcg                                                      <  1:2311483/101‑1 (MQ=255)                                             cccccGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCcgcg                                                    <  1:339974/101‑1 (MQ=255)                                                ccGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGcgccc                                                 <  1:2950362/101‑1 (MQ=255)                                                ccGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGcgccc                                                 <  2:2720052/101‑1 (MQ=255)                                                 cGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGcgcccg                                                <  1:1052647/101‑1 (MQ=255)                                                  gAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGGGGGGTCTGCCTCGGCTCCAGGACCCGCCACTCCGGCGGCCGGCCCCCcggcccc                                               >  2:2167779/1‑99 (MQ=255)                                                  gAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGcgcccgc                                               <  2:4236367/101‑1 (MQ=255)                                                   aGGGCGCCTCGGTCGCGGCGGGCGGACGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAACCCCGGCCGCAGCGGGGGACGGCCCCCcggccgcc                                              >  2:4666210/1‑101 (MQ=255)                                                     ggCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCCCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGcccc                                            <  1:1400710/101‑1 (MQ=255)                                                       cgcCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCcgg                                          <  1:588109/101‑1 (MQ=255)                                                        gcCTCTGTCGCGGCGGGCGGTCGCCTGTCGGTCCTGCCGCCGGGCTCTGCCGCGTCTCCACCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCcggc                                         <  1:2167934/101‑1 (MQ=255)                                                           tCGTTCGCGGCGGGCGGTCGCCTGGCGATCCTGGCGGCGTGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCggcggc                                      <  1:4666533/101‑1 (MQ=255)                                                                                cTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGC.GAAGGATGTCGagct                >  2:130027/1‑98 (MQ=255)                                                                                   ttgATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGC.GAAGGATGTCGAACATGc             <  1:125912/99‑1 (MQ=255)                                                                                     gATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCCGCCCCCGCGGCGGGCGCCCCCCCGCCCCCCCCCGGGGGGGCCGCGGAAGGA.GTCGAACAAGcca           >  2:125896/1‑100 (MQ=255)                                                                                           ggcggcGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGC.GAAGGATGTCGAACATGCCCCCgcgg     >  1:4792462/1‑101 (MQ=255)                                                                                              ggcggGCTCTGCCGCGGCTCCAGCCCCGGCCCCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGC.GGAGGATGTCGAACATGCCCCCGCGGCGa  >  1:4574935/1‑101 (MQ=255)                                                                                                    |                                                                                                CGAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGGTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGC.GAAGGATGTCGAACATGCCCCCGCGGCGA  >  NC_014034/797865‑798058 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG