Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	834,953	C→G	100%	P185R (CCC→CGC)	hoxH →	NAD‑reducing hydrogenase HoxS subunit beta

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	834,953	0	C→G	100.0%	236.1	66	P185R (CCC→CGC)	hoxH	NAD‑reducing hydrogenase HoxS subunit beta
Reads supporting (aligned to +/- strand):  new base (29/37):  ref base (0/0):  total (29/37)

ATGTCGCGCGCGCCTTTCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCCCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGATCGACTGGGCG  >  NC_014034/834857‑835050                                                                                                 |                                                                                                  aTGTCGCGCGCGCCTTTCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGgc                                                                                               <  1:3199751/101‑1 (MQ=255) aTGTCGCGCGCGCCTTTCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGgc                                                                                               <  2:5037659/101‑1 (MQ=255)    tCGCGCGCGCCTTTCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGGTGCGCGGggcg                                                                                            >  1:3571319/1‑101 (MQ=255)     cgcgcgcgCCTTTCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCgg                                                                                           <  2:3940422/101‑1 (MQ=255)      gcgcgcgcCTTTCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGGggg                                                                                          >  2:251515/1‑101 (MQ=255)       cgcgcgcCTTTCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCgggg                                                                                         <  1:1256827/101‑1 (MQ=255)         cgcgcCTTTCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGc                                                                                       <  2:2694813/101‑1 (MQ=255)         cgcgcCTTTCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGc                                                                                       <  2:2565026/101‑1 (MQ=255)                ttCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGGGGTGccc                                                                                >  2:2347360/1‑101 (MQ=255)                ttCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGccc                                                                                <  2:1624365/101‑1 (MQ=255)                 tCCGCAGCTGGTTACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCg                                                                               <  2:3977112/101‑1 (MQ=255)                 tCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCg                                                                               <  1:181248/101‑1 (MQ=255)                      aGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGc                                                                          <  1:415464/101‑1 (MQ=255)                       gCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCa                                                                         <  2:2522252/101‑1 (MQ=255)                       gCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGGTGCGCGGCGCGGGGGCGGGGCCCggggggc                                                                         >  1:4763195/1‑99 (MQ=255)                          ggCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATgc                                                                      <  2:192237/101‑1 (MQ=255)                          ggCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATgc                                                                      <  1:3235019/101‑1 (MQ=255)                          ggCGACCGAGGGGGTGCTGATGCGCCGCTTAGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATgc                                                                      <  1:2667024/101‑1 (MQ=255)                            cGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATgcgg                                                                    <  1:4229511/101‑1 (MQ=255)                             gACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATgcggc                                                                   <  2:1305705/101‑1 (MQ=255)                               ccGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGgcgc                                                                 <  2:4160512/101‑1 (MQ=255)                                cGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGcc                                                                <  1:3523486/101‑1 (MQ=255)                                 gAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGGTGCGCGGCGCGGGGGCGGGGCCCGGGGGCGTGCGGCGccc                                                               >  1:2528444/1‑101 (MQ=255)                                  aGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGGATGCGGGGCCCg                                                              >  1:1402779/1‑101 (MQ=255)                                  aGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCg                                                              <  2:3345313/101‑1 (MQ=255)                                  aGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCg                                                              >  2:796712/1‑101 (MQ=255)                                   gggggTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGGGCCCGGGGGCATGCGGCGCCCGc                                                             >  1:4933102/1‑101 (MQ=255)                                    ggggTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCt                                                            <  1:1770824/101‑1 (MQ=255)                                      ggTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGGGGCGCCCGCTGc                                                          <  1:2733258/101‑1 (MQ=255)                                      ggTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGc                                                          >  2:3722231/1‑101 (MQ=255)                                       gTGCTGATGCGCCGCTTCGGTCGGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGcc                                                         <  2:848048/101‑1 (MQ=255)                                       gTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGcc                                                         <  2:1911811/101‑1 (MQ=255)                                        tGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCcg                                                        <  1:2603335/101‑1 (MQ=255)                                            gATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGGGCCCGGGGGGATGCGGGGCCCCCTGCcgcgcc                                                    >  2:2296429/1‑100 (MQ=255)                                            gATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGGTGCGCGGCGCGGGGGCGGTGCCCGGGGGGATGCGGCGCCCGCTGCcgcgcg                                                    >  2:985107/1‑101 (MQ=255)                                             aTGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGTGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGTGCCCGCTGCcgcgcgc                                                   <  2:4033239/101‑1 (MQ=255)                                             aTGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCcgcgcgc                                                   <  2:3983427/101‑1 (MQ=255)                                                cgccgcTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGc                                                <  2:1334533/101‑1 (MQ=255)                                                 gccgcTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGcc                                               <  1:796814/101‑1 (MQ=255)                                                 gccgcTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCGGCCGCGCGCGGcc                                               >  1:4118890/1‑101 (MQ=255)                                                  ccgcTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCTCGCGCGGccc                                              <  2:1086709/101‑1 (MQ=255)                                                   cgcTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCcg                                             >  1:1659607/1‑101 (MQ=255)                                                    gcgtCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCcgc                                            <  2:3131887/98‑1 (MQ=255)                                                     cTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGGGCCCGGGGGGATGCGGGGCCCGCTGCCGCGCGCGGGCcgcg                                           >  2:1786033/1‑101 (MQ=255)                                                      ttCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGa                                          >  2:263537/1‑101 (MQ=255)                                                       tCGGGCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGAt                                         <  1:1863479/101‑1 (MQ=255)                                                         ggTCAGGAGGTGGTGCGGGCGATCCCCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCa                                       <  2:658065/101‑1 (MQ=255)                                                          gTCAGGAGGTGGTGCGGGCGATCACCGGCGGTCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAg                                      >  1:4479213/1‑101 (MQ=255)                                                          gTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAg                                      <  2:2528263/101‑1 (MQ=255)                                                          gTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGGGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGGCCGCGATCAg                                      >  1:4300018/1‑101 (MQ=255)                                                            cAGGAGGGGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCt                                    <  2:2925/101‑1 (MQ=255)                                                             aggaggTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTg                                   <  1:2347524/101‑1 (MQ=255)                                                               ggggtgggGCGGGCGATCCCCGGCGGGCGGTTGCGGGGCGCGGGGGCGGTGCCGGGGGGCAGGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTgcg                                 <  1:164409/99‑1 (MQ=255)                                                                aggTGGTGCGGGCGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTgcgc                                >  1:2327678/1‑101 (MQ=255)                                                                 ggtggtTCGGGCGATCACCGGGGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTgcgcg                               <  1:985217/101‑1 (MQ=255)                                                                   gggggCGGGCGATCCCCGGCGGGGGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCCCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGcc                             <  1:1981280/97‑1 (MQ=255)                                                                        ggggCGATCCCCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGt                        <  1:3722509/100‑1 (MQ=255)                                                                            cGATCACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGAt                    >  2:4678301/1‑101 (MQ=255)                                                                                cACCGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAgg                >  1:50356/1‑101 (MQ=255)                                                                                 aCCGGCGGGCGGTTGCGCGGCGCGGGGGGGGGGCCCGGGGGGATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGAGCAGCTGGGCGCCCATGGCGATCAggg               >  1:1807440/1‑100 (MQ=255)                                                                                  ccGGCGGGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTg              >  1:347587/1‑101 (MQ=255)                                                                                  ccGGCGGGCGGTTGCGCGGCGCCGGCGCCGTGCGCGGGGGCGCGCGGCCCCCGCTGCCGCGGGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTg              <  2:4812599/101‑1 (MQ=255)                                                                                   cggcggGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGa             <  2:4118573/101‑1 (MQ=255)                                                                                   cggcggGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGa             >  2:4610812/1‑101 (MQ=255)                                                                                   cggcggGCGGTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGGGa             >  2:4056761/1‑101 (MQ=255)                                                                                      cgggcggTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGATCg          >  1:3216814/1‑101 (MQ=255)                                                                                      cgggcggTGGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGATCg          <  2:2275227/101‑1 (MQ=255)                                                                                       gggcggTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGATCGa         >  2:213887/1‑101 (MQ=255)                                                                                       gggcggTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGATCGa         >  1:4224685/1‑101 (MQ=255)                                                                                         gcggTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGATCGACt       <  1:2296593/101‑1 (MQ=255)                                                                                          cggTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGATCGACTg      >  1:2535652/1‑101 (MQ=255)                                                                                           ggTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGATCGACTgg     >  1:1378509/1‑101 (MQ=255)                                                                                            gTTGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGATCGACTggg    >  1:1320115/1‑101 (MQ=255)                                                                                             ttGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGATCGACTgggg   >  2:58986/1‑100 (MQ=255)                                                                                              tGCGCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGATCGACTGggcg  >  1:5037026/1‑101 (MQ=255)                                                                                                 |                                                                                                  ATGTCGCGCGCGCCTTTCCGCAGCTGGCGACCGAGGGGGTGCTGATGCGCCGCTTCGGTCAGGAGGTGGTGCGGGCGATCACCGGCGGGCGGTTGCCCGGCGCGGGGGCGGTGCCCGGGGGCATGCGGCGCCCGCTGCCGCGCGCGGCCCGCGATCAGCTGCGCGCCGATGTCGATCAGGTGATCGACTGGGCG  >  NC_014034/834857‑835050 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG