Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,157,926 2 bp→AC 100% intergenic (‑298/‑483) RCAP_rcc02004 ← / → RCAP_rcc02005 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,157,9260C→A100.0% 54.1 244intergenic (‑298/‑484)RCAP_rcc02004/RCAP_rcc02005hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (99/125):  ref base (20/0):  total (119/125)
Fisher's exact test strand distribution p-value = 2.38e-07
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00
*NC_0140342,157,9270G→C100.0% 45.5 244intergenic (‑299/‑483)RCAP_rcc02004/RCAP_rcc02005hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (99/125):  ref base (20/0):  total (119/125)
Fisher's exact test strand distribution p-value = 2.38e-07
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

CTCCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.ACGGCCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCCGTCGT  >  NC_014034/2157834‑2158020
                                                                                             ||                                                                                             
 tcCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCgg                                                                                       <  2:3224128/101‑1 (MQ=255)
 tcCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCgg                                                                                       >  1:4547872/1‑101 (MQ=255)
 tcCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCgg                                                                                       <  2:4422283/101‑1 (MQ=255)
  cccAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGt                                                                                      >  1:4252858/1‑101 (MQ=255)
  cccAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGt                                                                                      >  2:279933/1‑101 (MQ=255)
    cAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTc                                                                                    <  1:4351776/101‑1 (MQ=255)
      gCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAg                                                                                  >  1:1613358/1‑101 (MQ=255)
      gCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGAGGAAAATC.AACGGCTTCGGTTCAg                                                                                  >  2:3550659/1‑101 (MQ=38)
       cATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGt                                                                                 <  1:2370676/101‑1 (MQ=255)
        aTACCAAACCGCTGGCGCCCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCCCAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTc                                                                                <  2:1820433/101‑1 (MQ=37)
        aTACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTc                                                                                <  1:690691/101‑1 (MQ=255)
         tACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTcc                                                                               <  2:2405959/101‑1 (MQ=255)
          aCCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTccg                                                                              <  2:3344723/101‑1 (MQ=255)
           ccAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTccgc                                                                             >  1:2818015/1‑101 (MQ=255)
            cAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTccgcc                                                                            >  1:4045539/1‑101 (MQ=255)
               aCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgtc                                                                         <  2:2904938/101‑1 (MQ=255)
                ctgCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgtcg                                                                        <  1:3421588/99‑1 (MQ=37)
                 cGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgtcgt                                                                       >  2:839193/1‑101 (MQ=255)
                 cGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgtcgt                                                                       <  1:1231964/101‑1 (MQ=255)
                  gCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCgtcgtc                                                                      >  2:4263678/1‑101 (MQ=255)
                    tGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATT.AACGGCTTCGGTTCAGTCCGCCGTCGTCtt                                                                    <  2:3980910/101‑1 (MQ=38)
                    tGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCtt                                                                    >  1:3604778/1‑101 (MQ=255)
                     ggCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTg                                                                   >  1:3235386/1‑101 (MQ=255)
                      gCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTgg                                                                  <  2:4471112/101‑1 (MQ=255)
                       cGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGc                                                                 <  1:1775412/101‑1 (MQ=255)
                        gACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCg                                                                >  1:3183023/1‑101 (MQ=255)
                           cATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTGGTCTTGGCGGTg                                                             <  1:2731712/101‑1 (MQ=38)
                            aTCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGa                                                            <  2:4787456/101‑1 (MQ=255)
                            aTCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGa                                                            >  2:1194113/1‑101 (MQ=255)
                             tCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGAc                                                           <  1:4790823/101‑1 (MQ=255)
                              cAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACg                                                          <  2:1655301/101‑1 (MQ=255)
                              cAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACg                                                          >  1:3972009/1‑101 (MQ=255)
                              cAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACg                                                          <  1:4093146/101‑1 (MQ=255)
                                  ttCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAg                                                      <  2:115572/101‑1 (MQ=255)
                                   tCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAgg                                                     <  1:1893197/101‑1 (MQ=255)
                                    cAGTCCGCCGTCGTCTTGGTGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGc                                                    <  1:1837151/101‑1 (MQ=38)
                                    cAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGTCGGGGACGTGGGGc                                                    >  2:4654574/1‑101 (MQ=37)
                                    cAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGc                                                    <  2:3797750/101‑1 (MQ=255)
                                    cAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGc                                                    <  2:3014282/101‑1 (MQ=255)
                                     aGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCa                                                   <  1:3469991/101‑1 (MQ=255)
                                      gTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGACCGCCGTCGTCTTGGCGGTGACGTGAGGCAc                                                  <  1:2852528/101‑1 (MQ=38)
                                        ccgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCt                                                >  1:2520607/1‑101 (MQ=255)
                                        ccgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCt                                                >  1:1734579/1‑101 (MQ=255)
                                        ccgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCt                                                >  2:510089/1‑101 (MQ=255)
                                         cgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTa                                               <  1:4163321/101‑1 (MQ=255)
                                         cgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTa                                               <  1:1482797/101‑1 (MQ=255)
                                          gccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTaa                                              <  1:839295/101‑1 (MQ=255)
                                           ccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTaaa                                             <  1:363746/101‑1 (MQ=255)
                                           ccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTaaa                                             <  1:1986045/101‑1 (MQ=255)
                                            cgtcgtCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAAc                                            <  1:639130/101‑1 (MQ=255)
                                             gtcgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACa                                           >  1:1033112/1‑101 (MQ=255)
                                               ggtcTTGGCGGTGATTTGATGCACAGGAGAAACCGATAAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAag                                         <  2:2377317/100‑1 (MQ=37)
                                                gtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAaga                                        <  1:1583688/101‑1 (MQ=255)
                                                gtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAaga                                        <  1:2195920/101‑1 (MQ=255)
                                                 tcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAagag                                       >  1:2024425/1‑101 (MQ=255)
                                                  cTTGGCGGTGATGTGATGCACAGGAGAGACGGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAgg                                      >  1:4494909/1‑101 (MQ=38)
                                                  cTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAgg                                      >  1:151973/1‑101 (MQ=255)
                                                  cTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAgg                                      >  2:4078141/1‑101 (MQ=255)
                                                     ggCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTc                                   <  1:4263995/101‑1 (MQ=255)
                                                      gCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCt                                  <  2:1786613/101‑1 (MQ=255)
                                                       cGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTa                                 <  1:1407595/101‑1 (MQ=255)
                                                       cGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTa                                 <  1:4379763/101‑1 (MQ=255)
                                                       cGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTa                                 <  2:1357982/101‑1 (MQ=255)
                                                        ggTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTAc                                <  1:2555269/101‑1 (MQ=255)
                                                         gtgatgtgatGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTTACGTGAGGCACCTAAACAAGAGGTTCTACg                               <  2:4547552/101‑1 (MQ=38)
                                                         gtgatgtgatGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACg                               >  1:2602144/1‑101 (MQ=255)
                                                         gtgatgtgatGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACg                               <  2:4324160/101‑1 (MQ=255)
                                                          tgatgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGa                              >  2:2267691/1‑101 (MQ=255)
                                                           gatgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                             >  2:1854919/1‑101 (MQ=255)
                                                           gatgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                             >  1:1028524/1‑101 (MQ=255)
                                                           gatgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                             <  1:2924727/101‑1 (MQ=255)
                                                           gatgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                             >  1:38398/1‑101 (MQ=255)
                                                            atgtgatgCACAGGAGAGACCGATTAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATg                            <  2:3906594/101‑1 (MQ=38)
                                                             tgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGc                           >  2:19776/1‑101 (MQ=255)
                                                             tgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGc                           <  2:1755913/101‑1 (MQ=255)
                                                              gtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGcg                          >  2:1487553/1‑100 (MQ=38)
                                                              gtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGcc                          <  1:4733232/101‑1 (MQ=255)
                                                               tgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                         >  1:539018/1‑101 (MQ=255)
                                                               tgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                         >  1:4822593/1‑101 (MQ=255)
                                                                gatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGa                        >  2:9360/1‑101 (MQ=255)
                                                                gatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGa                        >  2:1341560/1‑101 (MQ=255)
                                                                 atgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGaa                       <  1:2990037/101‑1 (MQ=255)
                                                                   gcccaGGAGAGACCGATTAAAATC.AACGGCTTCGGGTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAg                     <  1:2318448/98‑1 (MQ=37)
                                                                      caGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAgggg                  >  1:168339/1‑101 (MQ=255)
                                                                       aGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCGCCTAAACAAGAGGATCTACGATGCCGAAAGGGGt                 >  2:775386/1‑101 (MQ=37)
                                                                         gagagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTc               <  2:548208/101‑1 (MQ=255)
                                                                          agagaCCGATGAAAATC.AACGGCTTGGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCa              <  2:331036/101‑1 (MQ=38)
                                                                            agaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTTTACGATGCCGAAAGGGGTTCAGt            >  2:2172250/1‑101 (MQ=38)
                                                                             gaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTc           <  2:3170784/101‑1 (MQ=255)
                                                                              aCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTAAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTcc          >  2:5000858/1‑101 (MQ=38)
                                                                               ccGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccg         >  2:2662538/1‑101 (MQ=255)
                                                                               ccGATGAAAATC.AACGGCTTCGGTTCAGTCCCCCGTCGTCTTGGCGGTGACGTGAGGCACCTAACCAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccg         <  2:2520433/101‑1 (MQ=38)
                                                                                cGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccgc        >  1:2482273/1‑101 (MQ=255)
                                                                                 gATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccgcc       >  2:1725683/1‑101 (MQ=255)
                                                                                 gATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccgcc       >  2:3442977/1‑101 (MQ=255)
                                                                                  aTGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccgccg      <  1:5019044/101‑1 (MQ=255)
                                                                                  aTGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTATACGATGCCGAAAGGGGTTCAGTccgccg      >  1:1944136/1‑101 (MQ=38)
                                                                                   tGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgt     <  1:3806269/101‑1 (MQ=255)
                                                                                    gAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtc    <  1:4915374/101‑1 (MQ=255)
                                                                                     aaaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtcg   >  1:2395873/1‑101 (MQ=255)
                                                                                             ||                                                                                             
CTCCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.ACGGCCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCCGTCGT  >  NC_014034/2157834‑2158020

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG