Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,158 Δ1 bp 2.5% coding (40/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1580C→. 2.5% 11.2 0.0 ‑0.0280coding (40/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/3):  ref base (136/137):  total (140/140)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.60e-01

Only 100 of 367 total aligned reads displayed.

CGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1396058‑1396258
                                                                                                    |                                                                                                    
 gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCgccgcc                                                                                                     >  1:1472845/1‑101 (MQ=255)
 gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCgccgcc                                                                                                     <  1:6168175/101‑1 (MQ=255)
 gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCgccccc                                                                                                     >  2:435996/1‑101 (MQ=255)
  aaaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCa                                                                                                    >  1:3916225/1‑101 (MQ=255)
  aaaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCa                                                                                                    <  1:5104262/101‑1 (MQ=255)
    aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACg                                                                                                  >  2:956214/1‑101 (MQ=255)
    aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACg                                                                                                  >  2:6553864/1‑101 (MQ=255)
     cAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGc                                                                                                 >  2:4060873/1‑101 (MQ=255)
       ggCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCgccccccccccg                                                                                               >  1:1135623/1‑95 (MQ=255)
       ggCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTg                                                                                               <  2:211272/101‑1 (MQ=255)
         cgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCCCGCTGCg                                                                                             >  1:4859309/1‑101 (MQ=255)
         cgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCg                                                                                             <  2:4579898/101‑1 (MQ=255)
          gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCgg                                                                                            >  2:823637/1‑100 (MQ=255)
           cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTc                                                                                           <  2:678125/101‑1 (MQ=255)
            aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCCCGCTGCGTCg                                                                                          >  2:1148533/1‑101 (MQ=255)
            aTCGGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCGCGCGCCGCCACGCTGCGTCg                                                                                          >  1:6841523/1‑101 (MQ=255)
              cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCCCGCCGCGGCGcc                                                                                        >  1:6618906/1‑100 (MQ=255)
              cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCt                                                                                        <  2:4442102/101‑1 (MQ=255)
               tGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTc                                                                                       >  2:1629934/1‑101 (MQ=255)
               tGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTc                                                                                       >  2:4900942/1‑101 (MQ=255)
                gATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTcc                                                                                      >  1:6648922/1‑101 (MQ=255)
                gATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTcc                                                                                      <  1:3988859/101‑1 (MQ=255)
                 aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTccc                                                                                     >  2:1946763/1‑101 (MQ=255)
                 aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCCGCGTCGCTccc                                                                                     >  2:5460036/1‑101 (MQ=255)
                  tGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTcccc                                                                                    >  1:5970428/1‑101 (MQ=255)
                   gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTccccc                                                                                   <  1:2163460/101‑1 (MQ=255)
                   gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTcaccc                                                                                   <  1:5605324/101‑1 (MQ=255)
                    tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                                  <  1:849438/101‑1 (MQ=255)
                     atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCcg                                                                                 <  1:167191/101‑1 (MQ=255)
                      tatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCcgc                                                                                >  2:1503789/1‑101 (MQ=255)
                       atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCcgcg                                                                               >  1:5789222/1‑101 (MQ=255)
                            cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGc                                                                          >  1:6246682/1‑101 (MQ=255)
                              gTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCgg                                                                        >  1:5740862/1‑100 (MQ=255)
                               tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCCCCCCCCGCGGGGGCGTg                                                                       >  2:4020239/1‑101 (MQ=255)
                                 agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGGTCGCGCCGCCACGCTGGGGCGCTCCCCCCCCGGGGGCGGGGa                                                                     >  2:3288056/1‑101 (MQ=37)
                                 agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTggg                                                                     >  1:6513682/1‑100 (MQ=255)
                                 agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  1:3810071/1‑101 (MQ=255)
                                   gcgccctgcGCCCCCCCCGGGGGGGCGTGGAGCGAAGCTTTTCGGCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:3299122/93‑1 (MQ=37)
                                   aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:1230815/101‑1 (MQ=255)
                                    cGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:4061049/101‑1 (MQ=255)
                                     ggCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 <  2:7115773/101‑1 (MQ=255)
                                     gccTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 <  1:54668/99‑1 (MQ=255)
                                       cTGGCGCCCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               <  1:4706623/101‑1 (MQ=255)
                                        tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              >  1:6312556/1‑101 (MQ=255)
                                        tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              <  2:1236324/101‑1 (MQ=255)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                             >  1:953901/1‑101 (MQ=255)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCCCGCCGCCACGCTGCGTCGCCCCCCCCGCGGGGGGGTGGATCGAAAcc                                                             >  2:736957/1‑101 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCg                                                           >  1:5301632/1‑101 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCCCGCTGCGTCGCTCCCCCCGCGGGGGCGTGGAGCGCAAccccg                                                          >  1:2329858/1‑99 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCgg                                                          >  1:1121218/1‑101 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCgg                                                          <  2:1318028/101‑1 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCg                                                        >  2:2568470/1‑101 (MQ=255)
                                                  cccGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaaa                                                    <  1:4596321/101‑1 (MQ=255)
                                                   ccGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaaaa                                                   <  2:6648580/101‑1 (MQ=255)
                                                   ccGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaaaa                                                   <  1:135447/101‑1 (MQ=255)
                                                      cgGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                                                <  1:3458636/101‑1 (MQ=255)
                                                      cgGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAAgg                                                >  1:4506140/1‑101 (MQ=255)
                                                       gggggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGc                                               >  1:4098986/1‑101 (MQ=255)
                                                        ggggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGcc                                              >  2:4706483/1‑101 (MQ=255)
                                                        ggggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGcc                                              <  2:2929902/101‑1 (MQ=255)
                                                         gggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCa                                             >  2:6815711/1‑101 (MQ=255)
                                                          ggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                            >  2:1705860/1‑101 (MQ=255)
                                                           gCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATg                                           <  1:7416/101‑1 (MQ=255)
                                                            cGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGc                                          >  1:2767068/1‑101 (MQ=255)
                                                            cGTGGATCGAAACTTTTCGTCATCTGGCAGGCGCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGc                                          <  2:5282864/101‑1 (MQ=255)
                                                             gTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGcc                                         >  2:3022634/1‑101 (MQ=255)
                                                               ggATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                                       >  1:1573248/1‑101 (MQ=255)
                                                               ggATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                                       >  2:3866757/1‑101 (MQ=255)
                                                               ggATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                                       >  2:4212069/1‑101 (MQ=255)
                                                               ggATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGGTCGAAAACCGGGGAAAAACGGCCagggccc                                       >  2:5704360/1‑95 (MQ=38)
                                                                gATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTcc                                      >  1:6753524/1‑101 (MQ=255)
                                                                 aTCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCg                                     <  2:4774794/101‑1 (MQ=255)
                                                                    gAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGgt                                  <  1:2467610/101‑1 (MQ=255)
                                                                      aaCTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGgtgt                                <  2:703646/101‑1 (MQ=255)
                                                                         ttttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                             <  1:3107282/101‑1 (MQ=255)
                                                                         ttttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                             <  1:2961607/101‑1 (MQ=255)
                                                                          tttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg                            <  2:5807012/101‑1 (MQ=255)
                                                                           ttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGc                           <  2:1009000/101‑1 (MQ=255)
                                                                               tCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                       <  2:2365675/101‑1 (MQ=255)
                                                                                cATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                      <  1:5665196/101‑1 (MQ=255)
                                                                                cATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                      >  2:5097299/1‑101 (MQ=255)
                                                                                cATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTGGCTGTCg                      >  1:1857157/1‑101 (MQ=255)
                                                                                 aTCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGc                     >  2:5956320/1‑101 (MQ=255)
                                                                                        aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc              <  1:5156989/101‑1 (MQ=255)
                                                                                        aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc              <  2:518183/101‑1 (MQ=255)
                                                                                        aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc              <  1:6984135/101‑1 (MQ=255)
                                                                                        aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc              >  2:2235163/1‑101 (MQ=255)
                                                                                        aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc              <  1:2610860/101‑1 (MQ=255)
                                                                                        aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc              <  1:7095751/101‑1 (MQ=255)
                                                                                         ggCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcg             <  2:3699392/101‑1 (MQ=255)
                                                                                         ggCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcg             >  2:6742536/1‑101 (MQ=255)
                                                                                         ggCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcg             <  2:2031501/101‑1 (MQ=255)
                                                                                         ggCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAAGGCCATGCCTCCGGGGGTCTGGCTGTCGCTCCCCCcg             >  1:2274017/1‑101 (MQ=255)
                                                                                          gCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcgc            <  2:2766966/101‑1 (MQ=255)
                                                                                               cgccgcCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCggggg       <  1:2829158/101‑1 (MQ=255)
                                                                                                gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc      <  2:6202523/101‑1 (MQ=255)
                                                                                                gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc      <  2:5152461/101‑1 (MQ=255)
                                                                                                gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc      >  1:1024453/1‑101 (MQ=255)
                                                                                                   gccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTg   <  1:3692603/101‑1 (MQ=255)
                                                                                                    ccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTgg  <  2:3214306/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1396058‑1396258

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG