Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,201 Δ1 bp 100% intergenic (‑177/+99) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2010A→.100.0% 247.7 293intergenic (‑177/+99)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (116/120):  ref base (35/22):  total (151/142)
Fisher's exact test strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.49e-08

Only 100 of 320 total aligned reads displayed.

AGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640102‑2640297
                                                                                                      |                                                                                                
aGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGa                                                                                                  <  2:6868710/101‑1 (MQ=37)
  gCGTGTCAACGGTGGCGCATTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGG.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                                <  1:501542/101‑1 (MQ=25)
   ggtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                               <  1:2720101/100‑2 (MQ=25)
   cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                               <  1:2804442/101‑2 (MQ=25)
     tgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagac                                                                                             <  2:2659503/101‑4 (MQ=25)
          aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                       >  2:3070104/1‑101 (MQ=21)
             gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGtt                                                                                    <  1:3152657/101‑1 (MQ=25)
             gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGtt                                                                                    <  1:1689096/101‑1 (MQ=21)
             gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCCC.TT.GGCCTTCGGCCCATTCACCCCCCGAGGGATTTTGAGCAAGATG.AAAGACCATggggt                                                                                    >  2:911040/1‑99 (MQ=21)
              tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                   <  1:6533404/101‑1 (MQ=25)
              tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                   <  2:5572553/101‑1 (MQ=21)
                gcgtTTGTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTT.GT.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                 <  1:3866541/101‑1 (MQ=21)
                gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                 <  1:1389718/101‑1 (MQ=21)
                     tttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGc                                                                            <  2:5250840/101‑1 (MQ=25)
                     tttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGc                                                                            <  2:4059642/101‑1 (MQ=21)
                         cgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTTCCCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCCTCTTGCCTcc                                                                        >  2:5841859/1‑100 (MQ=21)
                         cgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                        <  1:5441201/101‑1 (MQ=25)
                           gccccgaaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                     >  1:4136595/7‑101 (MQ=14)
                           cAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                      <  2:5014294/101‑1 (MQ=25)
                           cAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                      <  1:4100142/101‑1 (MQ=21)
                            aGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                                     <  1:699049/101‑1 (MQ=21)
                             ggCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                    <  2:5519749/101‑1 (MQ=25)
                             cccgaaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                   >  1:5956748/5‑101 (MQ=17)
                              gCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                   >  2:2837541/1‑101 (MQ=21)
                              gCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                   >  1:1892074/1‑101 (MQ=21)
                               cAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTTCCTCaacaatc                                                                  >  2:4820428/1‑96 (MQ=18)
                               cAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATACACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                                  >  1:437305/1‑101 (MQ=21)
                                gaaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCCAATATccc                                                                >  1:5718541/2‑101 (MQ=17)
                                gaaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                >  2:3537200/2‑101 (MQ=17)
                                gaaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                <  2:1377202/100‑1 (MQ=17)
                                gaaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                >  2:1713952/2‑101 (MQ=17)
                                 aaaGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                                >  1:2109252/1‑101 (MQ=21)
                                 aaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               >  2:6220702/1‑101 (MQ=17)
                                 aaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               <  2:6772506/101‑1 (MQ=17)
                                 aaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               >  1:2922667/1‑101 (MQ=17)
                                  aaagggggCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              >  2:1974105/4‑101 (MQ=17)
                                  aaagggggCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              >  2:5647525/4‑101 (MQ=17)
                                   aagggggCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                             <  2:5761377/99‑1 (MQ=18)
                                    ggggggCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                             >  1:4425812/1‑101 (MQ=21)
                                      ggggCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                          <  2:6343180/101‑2 (MQ=18)
                                       gggCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                          >  1:6383273/1‑101 (MQ=25)
                                        ggCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                        <  2:2410834/101‑4 (MQ=21)
                                        ggCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                        >  1:873343/1‑98 (MQ=21)
                                         gcgcTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGCTTATCTTGCCCCAAATATCCCgggggtgag                                                       <  1:3673284/101‑5 (MQ=18)
                                           gcTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                     >  1:519870/1‑95 (MQ=21)
                                             tGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                    <  1:2035131/101‑1 (MQ=25)
                                             tGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                    <  2:3236123/101‑1 (MQ=25)
                                             tGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                    <  1:3548698/101‑1 (MQ=25)
                                             tGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCCGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGACCCAAATATCTCgggggtgaggcga                                                   >  2:2059231/1‑93 (MQ=21)
                                              gCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                   >  1:3318576/1‑101 (MQ=25)
                                              gCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                   <  1:6058896/101‑1 (MQ=37)
                                              gCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                   <  1:5842161/101‑1 (MQ=37)
                                              gCCCCCCTC.TT.GGCCTTCGGCCAATTAACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                   >  2:4188578/1‑101 (MQ=25)
                                                 ccccTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                >  2:338339/1‑101 (MQ=37)
                                                     tc.tT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                            >  2:2725146/1‑101 (MQ=37)
                                                           ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                        >  2:1186231/4‑101 (MQ=37)
                                                           ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                        >  1:5316573/4‑101 (MQ=37)
                                                            gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                       <  1:1052432/99‑1 (MQ=37)
                                                             ccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                      >  1:1275586/2‑101 (MQ=37)
                                                             ccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                      >  2:6658813/2‑101 (MQ=37)
                                                              cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                     >  2:3661989/1‑101 (MQ=255)
                                                              cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                     <  1:6804276/101‑1 (MQ=255)
                                                               ttCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                                    <  2:6313213/101‑1 (MQ=255)
                                                                 cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                  >  2:257496/1‑101 (MQ=255)
                                                                   gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                >  2:653908/1‑101 (MQ=37)
                                                                    gccatttcccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                               <  1:998709/93‑1 (MQ=37)
                                                                    ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGTGGGCAGAGcc                               <  2:472382/101‑1 (MQ=37)
                                                                     cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                              >  2:2934627/1‑101 (MQ=37)
                                                                      aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             >  2:4453829/1‑101 (MQ=255)
                                                                       aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                            >  1:5547709/1‑101 (MQ=37)
                                                                         tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                          >  1:1151113/4‑101 (MQ=25)
                                                                         tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                          >  2:6000430/4‑101 (MQ=25)
                                                                            ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                       <  2:5907108/101‑1 (MQ=255)
                                                                              ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                     >  1:5371678/1‑101 (MQ=255)
                                                                                 cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                  >  2:7044362/1‑101 (MQ=255)
                                                                                  gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                 >  1:2820844/1‑101 (MQ=255)
                                                                                  gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                 <  2:1054194/101‑1 (MQ=255)
                                                                                    ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt               >  2:4316225/1‑101 (MQ=255)
                                                                                    ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt               >  1:4821543/1‑101 (MQ=255)
                                                                                    ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt               >  1:805513/1‑101 (MQ=255)
                                                                                     gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc              >  1:5484814/1‑101 (MQ=255)
                                                                                     gTATTTGGAGCAAGATG.AAAGACCAGGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc              <  2:3894719/101‑1 (MQ=255)
                                                                                      tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             <  1:6242969/101‑1 (MQ=255)
                                                                                      tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  2:6047102/1‑101 (MQ=255)
                                                                                      tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             <  1:1229927/101‑1 (MQ=255)
                                                                                       aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc            >  1:3505988/1‑101 (MQ=255)
                                                                                       aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc            >  2:5093612/1‑101 (MQ=255)
                                                                                       aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGGCTTtctc            >  1:4080715/1‑101 (MQ=255)
                                                                                        tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           >  1:2686809/1‑101 (MQ=255)
                                                                                        tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           >  1:3427672/1‑101 (MQ=255)
                                                                                        tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           <  2:6178576/101‑1 (MQ=255)
                                                                                            tcttccgATCAAAAGCCCGTGGGTTCATCTGGCCTAAAATACCCCGGGGGGAGTCCGGAAGGGGGGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        <  1:7090863/95‑1 (MQ=21)
                                                                                             agcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGt      <  1:6659155/99‑1 (MQ=255)
                                                                                             agcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGt      >  2:1213591/3‑101 (MQ=255)
                                                                                              gcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGtt     <  2:1945525/100‑1 (MQ=255)
                                                                                               cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc    <  1:743555/101‑1 (MQ=255)
                                                                                                taGATG.AAAGACCATGGTTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc   <  1:6327200/100‑1 (MQ=255)
                                                                                                aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc   <  1:2275051/101‑1 (MQ=255)
                                                                                                 aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  <  2:540408/101‑1 (MQ=255)
                                                                                                 aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  <  2:805489/101‑1 (MQ=255)
                                                                                                      |                                                                                                
AGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640102‑2640297

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG