Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,024,766 Δ1 bp 100% intergenic (‑176/‑152) chvI ← / → pckA transcriptional regulator ChvI/phosphoenolpyruvate carboxykinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,024,7660T→.100.0% 1671.6 381intergenic (‑176/‑152)chvI/pckAtranscriptional regulator ChvI/phosphoenolpyruvate carboxykinase
Reads supporting (aligned to +/- strand):  new base (191/188):  ref base (0/0):  total (193/188)

Only 100 of 420 total aligned reads displayed.

TGCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGTTTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTGCCCGCCC  >  NC_014034/3024666‑3024862
                                                                                                    |                                                                                                
tgCTAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGt                                                                                                  <  2:915516/101‑1 (MQ=255)
tgCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGt                                                                                                  >  1:3774808/1‑101 (MQ=255)
tgCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGt                                                                                                  >  1:2773400/1‑101 (MQ=255)
 gCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtt                                                                                                 <  1:3980062/101‑1 (MQ=255)
 gCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtt                                                                                                 <  1:3143244/101‑1 (MQ=255)
  cGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGttt                                                                                                <  1:413668/101‑1 (MQ=255)
  cGAATCGCCCACGTGATGCATATTAGCTGAGAGAAAAAAAACGAAGGTTTGACTAAGTTGTCTCACCGCTTCGAAATGATGGGGATAAAACGGCACTGttt                                                                                                <  2:2026601/101‑1 (MQ=255)
   gAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttg                                                                                               >  1:6154166/1‑100 (MQ=255)
   gAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttg                                                                                               >  1:828047/1‑100 (MQ=255)
   gAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttg                                                                                               >  1:2957153/1‑100 (MQ=255)
    aaTCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgc                                                                                              >  1:5472618/1‑99 (MQ=255)
    aaTCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgc                                                                                              >  1:5188668/1‑99 (MQ=255)
    aaTCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgc                                                                                              <  1:3661688/101‑3 (MQ=255)
     aTCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgcg                                                                                             <  1:5932604/101‑4 (MQ=255)
      tCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgcgc                                                                                            >  2:7056551/1‑97 (MQ=255)
      tCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgcgc                                                                                            <  2:6878450/101‑5 (MQ=255)
       cGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCt                                                                                          <  1:5588118/101‑1 (MQ=255)
         cccACGTGATGCGAATCAACTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAg                                                                                        <  2:6155602/101‑1 (MQ=255)
           cACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCt                                                                                      <  1:5419219/101‑1 (MQ=255)
            aCGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTg                                                                                     >  1:4662014/1‑101 (MQ=255)
            aCGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTCGCGCTAGCTg                                                                                     >  2:4845998/1‑101 (MQ=255)
             cGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGc                                                                                    >  2:1786073/1‑101 (MQ=255)
                 aTGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCtagatcg                                                                                >  1:779489/1‑95 (MQ=255)
                  tGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACaa                                                                               <  2:4594572/101‑1 (MQ=255)
                    cGAATGATCTGAGCGGAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAAc                                                                             >  1:6867063/1‑101 (MQ=255)
                    cGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAAc                                                                             >  1:1928559/1‑101 (MQ=255)
                     gAATCATCTGAGCGCAAAAAAACGAATGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAAcc                                                                            <  2:627200/101‑1 (MQ=255)
                     gAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTGGCTGCGACAAAcc                                                                            <  1:1473463/101‑1 (MQ=255)
                     gAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAAcc                                                                            >  1:2816345/1‑101 (MQ=255)
                      aaTCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAAccc                                                                           >  2:7100245/1‑101 (MQ=255)
                       atcatcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCt                                                                          >  1:2656190/1‑101 (MQ=255)
                         catcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTc                                                                        <  1:1825468/101‑1 (MQ=255)
                          atcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCt                                                                       >  1:6630261/1‑101 (MQ=255)
                          atcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCt                                                                       >  1:4341870/1‑101 (MQ=255)
                          atcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCt                                                                       >  2:683624/1‑101 (MQ=255)
                             tGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGtt                                                                    <  1:6251551/101‑1 (MQ=255)
                             tGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGtt                                                                    <  2:2195055/101‑1 (MQ=255)
                             tGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGtt                                                                    <  1:1016627/101‑1 (MQ=255)
                              gAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTc                                                                   <  2:4126975/101‑1 (MQ=255)
                              gAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTc                                                                   >  1:4460695/1‑101 (MQ=255)
                               aGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTcc                                                                  <  1:739878/101‑1 (MQ=255)
                               aGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCT.ATTTGCGCTAGCTGCGACAAACCCTTCTGTTcc                                                                  >  1:3546283/1‑101 (MQ=255)
                                 cgcAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCtt                                                                <  2:4746458/101‑1 (MQ=255)
                                   cAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCg                                                              >  1:1224480/1‑101 (MQ=255)
                                   cAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCg                                                              <  1:1372501/101‑1 (MQ=255)
                                      aaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCaa                                                           >  1:3214205/1‑101 (MQ=255)
                                       aaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAt                                                          <  2:2801910/101‑1 (MQ=255)
                                         aaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTg                                                        <  1:4706066/101‑1 (MQ=255)
                                          agcaaGGTTTGTCTAAACTGACGCACCGCTTCGCGATGATGGCGGTAAATCGCCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTgc                                                       <  2:3106481/98‑1 (MQ=255)
                                           cGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTgcg                                                      >  2:5282264/1‑101 (MQ=255)
                                            gAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTgcgc                                                     >  1:7024587/1‑101 (MQ=255)
                                             aaGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGcc                                                    <  1:4073766/101‑1 (MQ=255)
                                             aaGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTACCTGAGAAAAACCCTTCTGTTCCTTCGCAAttcgtcc                                                    >  2:2312418/1‑96 (MQ=255)
                                              aGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCa                                                   <  2:1413087/101‑1 (MQ=255)
                                              aGGTCTGCCTAGTCTGTCTGCGCTCTTCGCGATGATGGCGCTAGATCGGCGCTG.TTTGCGCTAGCTGCGACAAGCCCTTCTGTTCCTTCGCAATTGCGCCa                                                   <  2:1168177/101‑1 (MQ=255)
                                                gTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATg                                                 >  2:3886969/1‑101 (MQ=255)
                                                 tttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATgg                                                <  1:6417222/101‑1 (MQ=255)
                                                 tttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATgg                                                >  1:2544874/1‑101 (MQ=255)
                                                  ttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCaagat                                               >  2:4984314/1‑97 (MQ=255)
                                                  ttGACTAATTTGTATCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGt                                               >  2:1902246/1‑101 (MQ=255)
                                                    gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                             >  2:2478502/1‑101 (MQ=255)
                                                    gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                             <  2:3143820/101‑1 (MQ=255)
                                                      cTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAg                                           <  2:5188408/101‑1 (MQ=255)
                                                       tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                          >  2:5393725/1‑101 (MQ=255)
                                                        aaTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAggg                                         >  2:3422670/1‑101 (MQ=255)
                                                        aaTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAggg                                         >  1:7096866/1‑101 (MQ=255)
                                                         aTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgc                                        >  2:252115/1‑101 (MQ=255)
                                                         aTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgc                                        >  1:913140/1‑101 (MQ=255)
                                                           ttGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgcgc                                      >  1:4941005/1‑101 (MQ=255)
                                                            tGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgcgca                                     >  2:2767214/1‑100 (MQ=255)
                                                            tGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGcc                                     <  2:1369064/101‑1 (MQ=255)
                                                            tGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGcc                                     >  2:4439549/1‑101 (MQ=255)
                                                             gTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCa                                    <  2:2995618/101‑1 (MQ=255)
                                                              tctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCAt                                   <  2:6050931/101‑1 (MQ=255)
                                                               ctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATc                                  >  1:2206253/1‑101 (MQ=255)
                                                                tcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATcc                                 >  2:6393663/1‑101 (MQ=255)
                                                                 cACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCg                                >  2:4356156/1‑101 (MQ=255)
                                                                  aCCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGc                               >  1:5740056/1‑101 (MQ=255)
                                                                        tCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACAtt                         <  2:1512295/101‑1 (MQ=255)
                                                                           cgATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCtt                      >  2:6692483/1‑101 (MQ=255)
                                                                           cgATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCtt                      >  1:1628874/1‑101 (MQ=255)
                                                                           cgATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCtt                      <  2:6999049/101‑1 (MQ=255)
                                                                            gatgatGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTg                     <  1:5311345/101‑1 (MQ=255)
                                                                            gatgatGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTg                     <  2:4093680/101‑1 (MQ=255)
                                                                            gatgaaGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTg                     >  2:3514961/1‑101 (MQ=255)
                                                                             atgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgc                    <  2:5869353/101‑1 (MQ=255)
                                                                             atgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgc                    <  2:3691671/101‑1 (MQ=255)
                                                                              tgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgcg                   >  2:506047/1‑101 (MQ=255)
                                                                              tgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgcg                   >  1:1981276/1‑101 (MQ=255)
                                                                              tgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgcg                   >  1:3076710/1‑101 (MQ=255)
                                                                                 tgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCgg                >  2:1243900/1‑101 (MQ=255)
                                                                                 tgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCgg                >  1:5808658/1‑101 (MQ=255)
                                                                                  ggCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCACCATTCTTGCCCggc               >  1:2310936/1‑100 (MQ=255)
                                                                                    cgcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATc             <  2:5810518/101‑1 (MQ=255)
                                                                                      cTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCgg           <  2:5668401/101‑1 (MQ=255)
                                                                                       tAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGt          >  1:6779343/1‑101 (MQ=255)
                                                                                        aaaTCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTg         >  2:2651778/1‑101 (MQ=255)
                                                                                        aaaTCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTg         >  1:3377610/1‑101 (MQ=255)
                                                                                              gCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcccgcc   >  2:3775561/1‑101 (MQ=255)
                                                                                               cccTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcccgccc  <  2:801795/101‑1 (MQ=255)
                                                                                                    |                                                                                                
TGCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGTTTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTGCCCGCCC  >  NC_014034/3024666‑3024862

Base quality scores:  ATCG  < 3 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG