Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,768,902 +G 97.2% intergenic (+29/‑27) lpxA → / → RCAP_rcc01636 acyl‑ACP‑‑UDP‑N‑acetylglucosamine O‑acyltransferase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,768,9021.→G97.2% 12.2 ‑0.3 ‑1.4249intergenic (+29/‑27)lpxA/RCAP_rcc01636acyl‑ACP‑‑UDP‑N‑acetylglucosamine O‑acyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (127/115):  ref base (3/4):  total (130/119)
Fisher's exact test strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.67e-02

Only 100 of 288 total aligned reads displayed.

AGCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.C.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGTGCT  >  NC_014034/1768802‑1768996
                                                                                                      |                                                                                              
agCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGc.c                                                                                                 >  2:4988034/1‑101 (MQ=255)
agCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGc.c                                                                                                 <  1:4319700/101‑1 (MQ=255)
agCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGc.c                                                                                                 <  2:6565639/101‑1 (MQ=255)
 gccgccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGCTGAGCGCGGGATCTGAAACCGCGCTGATCGC.C.g                                                                                               <  1:3706067/101‑1 (MQ=255)
    gccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.C.gggg                                                                                            >  1:5176766/1‑101 (MQ=255)
     ccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.C.ggggg                                                                                           >  1:3854465/1‑101 (MQ=255)
     ccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.C.ggggg                                                                                           <  1:1601443/101‑1 (MQ=255)
       aCGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.C.ggggggg                                                                                         <  1:6805110/101‑1 (MQ=255)
         gTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.C.ggggggggc                                                                                       >  2:2441628/1‑99 (MQ=255)
         gTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.C.ggggggggc                                                                                       <  2:4850274/101‑3 (MQ=255)
           ccGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.C.ggggggggcgc                                                                                     <  2:5573793/101‑5 (MQ=255)
              cgAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCt                                                                                   <  2:4335716/101‑1 (MQ=255)
              cgAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCt                                                                                   >  2:1110538/1‑101 (MQ=255)
               gAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTg                                                                                  >  1:243787/1‑101 (MQ=255)
               gAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTg                                                                                  >  2:4639434/1‑101 (MQ=255)
                aaGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGc                                                                                 >  1:714935/1‑101 (MQ=255)
                aaGTGCCTGATTTCATTCTTGGTCCCTCGGACCTCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCTC.CGGGGGGGGCGCGCTGc                                                                                 <  1:2001907/101‑1 (MQ=255)
                 aGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGTGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGcc                                                                                <  2:3388155/101‑1 (MQ=255)
                 aGTGCTTGATTTCATTCTTGGGCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGcc                                                                                <  2:6343551/101‑1 (MQ=255)
                  gTGCTTGATTTCATTCTTGGCCCCTCGGCCCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCg                                                                               <  1:5058598/101‑1 (MQ=255)
                     cTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCgggg                                                                            >  2:5151229/1‑101 (MQ=255)
                       tGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGgct                                                                          >  1:1736234/1‑101 (MQ=255)
                       tGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGgct                                                                          >  1:143019/1‑101 (MQ=255)
                       tGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGgct                                                                          >  1:1359569/1‑101 (MQ=255)
                       tGATTTCATTCTTGGCCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGgct                                                                          <  1:3395404/101‑1 (MQ=255)
                        gATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGgctg                                                                         >  2:3758403/1‑101 (MQ=255)
                        gATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGgctg                                                                         >  1:1019975/1‑101 (MQ=255)
                        gATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGgctg                                                                         >  2:806467/1‑101 (MQ=255)
                         aTTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGgctgc                                                                        <  1:5947789/101‑1 (MQ=255)
                          tttCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGgctgct                                                                       <  2:5223271/101‑1 (MQ=255)
                          tttCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGgctgct                                                                       >  1:1051218/1‑101 (MQ=255)
                          tttCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGgctgct                                                                       >  1:7030202/1‑101 (MQ=255)
                           ttCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCtt                                                                      >  2:311572/1‑101 (MQ=255)
                            tCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCttt                                                                     >  1:3359966/1‑101 (MQ=255)
                            tCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCttt                                                                     <  2:6864485/101‑1 (MQ=255)
                             cATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCtttt                                                                    <  2:1488684/101‑1 (MQ=255)
                             cATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCtttt                                                                    <  1:4913315/101‑1 (MQ=255)
                              aTTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTg                                                                   <  2:3559900/101‑1 (MQ=255)
                               ttCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGc                                                                  <  2:3841722/101‑1 (MQ=255)
                                tCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCa                                                                 <  2:4226198/101‑1 (MQ=255)
                                 cTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAg                                                                <  1:6442641/101‑1 (MQ=255)
                                 cTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAg                                                                >  1:2993579/1‑101 (MQ=255)
                                    ggTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGcc                                                             >  1:6540934/1‑101 (MQ=255)
                                    ggTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGcc                                                             >  2:4542351/1‑101 (MQ=255)
                                    ggTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGcc                                                             >  1:4032773/1‑101 (MQ=255)
                                    ggTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGcc                                                             >  2:5334152/1‑101 (MQ=255)
                                      tCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCcgc                                                           <  2:2096126/101‑1 (MQ=255)
                                       cccTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCcgcg                                                          >  1:2836075/1‑101 (MQ=255)
                                           cGGCCCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGCGG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCgg                                                      <  1:2563139/101‑1 (MQ=255)
                                           cGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCgg                                                      >  1:4248751/1‑101 (MQ=255)
                                           cGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCgg                                                      >  2:2220841/1‑101 (MQ=255)
                                            ggACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGa                                                     <  2:7120311/101‑1 (MQ=255)
                                             gACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGaa                                                    <  2:1476041/101‑1 (MQ=255)
                                              aCCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGaaa                                                   >  2:47561/1‑101 (MQ=255)
                                                cGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGCAATg                                                 >  2:289391/1‑101 (MQ=255)
                                                cGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATg                                                 >  2:5429453/1‑101 (MQ=255)
                                                cGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATg                                                 <  2:3043595/101‑1 (MQ=255)
                                                cGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCAGCCGGGGCTGCTTTTGCAGGCGGCGCCGGaaagg                                                 >  1:2091782/1‑99 (MQ=255)
                                                 gCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGc                                                >  1:6285620/1‑101 (MQ=255)
                                                 gCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGc                                                >  1:4593688/1‑101 (MQ=255)
                                                 gCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGc                                                >  1:4853729/1‑101 (MQ=255)
                                                     cTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTggc                                            >  2:6394257/1‑100 (MQ=255)
                                                      ttCCTGACGCCGCGATGAGCCCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTCGTc                                           <  1:6844947/101‑1 (MQ=255)
                                                       tCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCt                                          <  1:5346909/101‑1 (MQ=255)
                                                        ccTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTg                                         >  2:1938932/1‑101 (MQ=255)
                                                         cTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgc                                        >  1:5470135/1‑101 (MQ=255)
                                                         cTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgc                                        >  2:5794311/1‑101 (MQ=255)
                                                           gACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcgc                                      <  2:1002957/101‑1 (MQ=255)
                                                           gACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcgc                                      >  2:7009362/1‑101 (MQ=255)
                                                              gccgcGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCt                                   >  1:4436248/1‑101 (MQ=255)
                                                              gccgcGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCt                                   <  1:5670898/101‑1 (MQ=255)
                                                               ccgcGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTg                                  <  1:5289639/101‑1 (MQ=255)
                                                               ccgcGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTg                                  <  2:1736170/101‑1 (MQ=255)
                                                                cgcgAGGAGCGCGGGATCTGAACCCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcgcaccca                                 <  2:2500468/101‑6 (MQ=255)
                                                                 gcgATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGa                                <  2:1609066/101‑1 (MQ=255)
                                                                  cgATGAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGaa                               >  2:5995593/1‑101 (MQ=255)
                                                                      gAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTc                           <  1:806491/101‑1 (MQ=255)
                                                                      gAGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTc                           >  2:3225940/1‑101 (MQ=255)
                                                                       aGCGCGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCa                          >  2:5246528/1‑101 (MQ=255)
                                                                        gcgcGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAg                         >  2:644092/1‑101 (MQ=255)
                                                                        gcgcGGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAg                         <  2:2742/101‑1 (MQ=255)
                                                                           cgGGATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGccc                      <  1:843721/101‑1 (MQ=255)
                                                                            gggATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCcg                     <  1:319902/101‑1 (MQ=255)
                                                                             ggATCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCcgc                    >  2:38936/1‑101 (MQ=255)
                                                                               aTCTGAAACCGCGCTGATCGC.CGGGGGGGGGGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGCAGGTCAGCCcgccc                  >  2:4427353/1‑99 (MQ=255)
                                                                                tCTGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGcc                 <  2:1856164/101‑1 (MQ=255)
                                                                                  tGAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCgg               >  1:6173376/1‑101 (MQ=255)
                                                                                   gAAACCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCggg              >  2:5887523/1‑101 (MQ=255)
                                                                                    aaaCCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGc             <  1:1292958/101‑1 (MQ=255)
                                                                                    aaaCCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGc             <  1:2220936/101‑1 (MQ=255)
                                                                                     aaCCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCg            >  2:1330244/1‑101 (MQ=255)
                                                                                      aCCGCGCTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCgg           <  1:67682/101‑1 (MQ=255)
                                                                                         gcgcTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATc        >  1:1100594/1‑101 (MQ=255)
                                                                                         gcgcTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCACGCGCCGGGCGGATc        >  2:6227443/1‑101 (MQ=255)
                                                                                         gcgcTGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGCCAGCCCGCGCCGGGCGGATc        >  2:261075/1‑101 (MQ=255)
                                                                                            cTGATCGCGG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGt     <  2:227680/101‑1 (MQ=255)
                                                                                             tGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGGCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGtg    >  2:3440623/1‑101 (MQ=255)
                                                                                             tGATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGtg    >  2:936104/1‑101 (MQ=255)
                                                                                              gATCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGtgc   <  1:3444117/101‑1 (MQ=255)
                                                                                               aTCGC.CGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGtgct  <  1:47562/101‑1 (MQ=255)
                                                                                                      |                                                                                              
AGCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATCGC.C.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGTGCT  >  NC_014034/1768802‑1768996

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG