BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,843,426	+G	100%	intergenic (+88/+60)	ppiB → / ← tyrS	peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,843,426	1	.→G	100.0%	911.1	288	intergenic (+88/+60)	ppiB/tyrS	peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): new base (155/131): ref base (1/1): total (156/132)

Only 100 of 304 total aligned reads displayed.


CGATCGAGTGACACGGGCGC.A.GGGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCC.GGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGCTTCTT  >  NC_014034/1843328‑1843518


cGATCGAGTGACACGGGCGC.AGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCt                                                                                                  <  2:3201131/101‑1 (MQ=25)


 gagcGAGTGACACGGGCGC.AGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.g                                                                                                <  2:6362063/98‑1 (MQ=21)


      aGTGACACGGGCGC.AGGGGGGCTCTGCCCCCCTCCCC.TGCGCATTCCCCCCCCCGGCAATTTTCGCCAAGGCGAAATGGAAAAGGGCGCTCCGCT.gggggc                                                                                           >  2:577299/1‑99 (MQ=255)


      aGTGACACGGGCGC.AGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggc                                                                                           <  2:5287485/101‑3 (MQ=21)


         gACACGGGCGCAA.GGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggcgcc                                                                                        >  2:2540654/1‑96 (MQ=18)


           cacGGGCGC.AGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCt                                                                                      <  2:680853/101‑1 (MQ=21)


              gggCGC.AGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTc                                                                                   <  2:373887/101‑1 (MQ=21)


              gggCGC.AGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTc                                                                                   <  1:6893035/101‑1 (MQ=21)


              gggCGC.AGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTc                                                                                   <  1:929904/101‑1 (MQ=21)


               ggCGC.AGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCa                                                                                  <  1:1586057/101‑1 (MQ=21)


               ggCGC.AGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCa                                                                                  <  1:4870677/101‑1 (MQ=21)


                   gcaggggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCt                                                                               <  1:3491802/97‑1 (MQ=21)


                     aggggggCTCTG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTg                                                                             <  1:6988301/99‑1 (MQ=21)


                       gggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGcc                                                                           >  2:4886391/1‑101 (MQ=21)


                        ggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCca                                                                          >  1:4594834/1‑101 (MQ=25)


                               ctgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAata                                                                    >  1:1763090/4‑101 (MQ=25)


                                cgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAatat                                                                   <  1:1917805/99‑1 (MQ=37)


                                  ccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATcc                                                                 <  2:1432875/101‑1 (MQ=37)


                                  ccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATcc                                                                 <  2:2790387/101‑1 (MQ=37)


                                   cccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATccc                                                                <  1:6119968/101‑1 (MQ=37)


                                       ctctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCgg.gg                                                           >  2:6473953/7‑101 (MQ=37)


                                        tctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGt                                                          <  1:3920023/96‑1 (MQ=38)


                                         tccgatctcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CACCCTTTCATCTTGCCACAAATATCCCGG.GGTc                                                         <  1:5432238/93‑1 (MQ=37)


                                          tgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTCTCAGCTTGCCACAAATATCCCGG.GGtgc                                                        >  2:7034623/4‑99 (MQ=37)


                                           gctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCC.g                                                      >  1:6455625/3‑101 (MQ=38)


                                           gctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCC.g                                                      >  1:5212488/3‑101 (MQ=38)


                                               cgcATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCC.ggggc                                                  <  2:5049091/101‑3 (MQ=37)


                                                gcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCC.ggggca                                                 >  2:3230460/1‑98 (MQ=38)


                                                gcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCATTTCATCTTGCCACAAATATCCCGG.GGTCC.ggggca                                                 >  1:6406630/1‑98 (MQ=37)


                                                 cATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTcagatcgga                                                 >  2:5431966/1‑93 (MQ=37)


                                                  aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCC.ggggcaga                                               >  2:5627970/1‑96 (MQ=37)


                                                     cACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGcc                                             >  2:4511388/1‑101 (MQ=25)


                                                      aCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCNACAAATATCCCGG.GGTCCGGGGCAGAGccc                                            >  1:499281/1‑101 (MQ=25)


                                                       ccccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGcccc                                           <  1:1345660/101‑1 (MQ=25)


                                                        gccgccggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCGCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCg                                          <  1:2262563/95‑1 (MQ=21)


                                                        cccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCg                                          <  1:2409859/101‑1 (MQ=25)


                                                         ccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCgg                                         <  2:1932420/101‑1 (MQ=25)


                                                            cGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCt                                      <  1:6582981/101‑1 (MQ=25)


                                                             gggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCtt                                     >  1:437554/1‑101 (MQ=25)


                                                             gggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCtt                                     <  2:4552630/101‑1 (MQ=25)


                                                             gggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCtt                                     <  2:1525306/101‑1 (MQ=25)


                                                             gggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCtt                                     <  1:4846467/101‑1 (MQ=25)


                                                              ggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCCCAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTg                                    <  1:6325883/101‑1 (MQ=25)


                                                              ggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTg                                    >  2:1377817/1‑101 (MQ=25)


                                                              ggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTg                                    >  1:6172424/1‑101 (MQ=25)


                                                              ggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTg                                    >  2:2660188/1‑101 (MQ=25)


                                                               gATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGc                                   >  2:4316270/1‑101 (MQ=25)


                                                                atatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCt                                  >  2:1868726/1‑101 (MQ=25)


                                                                atatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCt                                  >  1:3066589/1‑101 (MQ=25)


                                                                  tttttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCa                                <  1:6465546/100‑1 (MQ=25)


                                                                  atTCTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCTGCCTTGCTCa                                >  2:1733349/1‑101 (MQ=21)


                                                                    tttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGc                              >  2:2868906/1‑101 (MQ=25)


                                                                    tttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGc                              >  2:617562/1‑101 (MQ=25)


                                                                     ttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGcc                             >  2:6229977/1‑101 (MQ=25)


                                                                     ttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGcc                             >  1:3758980/1‑101 (MQ=25)


                                                                      tCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGccc                            >  2:3004122/1‑101 (MQ=25)


                                                                      tCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGccc                            <  1:3297199/101‑1 (MQ=25)


                                                                      tCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGccc                            <  1:415374/101‑1 (MQ=25)


                                                                       cGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCa                           <  1:354031/101‑1 (MQ=25)


                                                                         ccAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCaga                         >  1:57366/1‑101 (MQ=25)


                                                                          cAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCagag                        >  1:841321/1‑101 (MQ=25)


                                                                          cAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCagag                        >  2:11868/1‑101 (MQ=25)


                                                                          cAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCagag                        <  2:4161295/101‑1 (MQ=25)


                                                                           aaGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAgaga                       >  2:5179122/1‑101 (MQ=25)


                                                                           aaGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAgaga                       >  1:5457205/1‑101 (MQ=25)


                                                                            aGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGac                      >  2:6184501/1‑101 (MQ=25)


                                                                             ggTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGaca                     >  2:6004683/1‑101 (MQ=25)


                                                                              gTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCTG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGacac                    <  2:3451819/101‑1 (MQ=25)


                                                                               tGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACAcc                   >  1:2826808/1‑101 (MQ=25)


                                                                               tGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACAcc                   >  1:3177347/1‑101 (MQ=25)


                                                                               tGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACAcc                   >  2:3996778/1‑101 (MQ=25)


                                                                                gAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa                  >  1:988943/1‑101 (MQ=25)


                                                                                gAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa                  >  2:3156398/1‑101 (MQ=25)


                                                                                gAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa                  <  2:6054837/101‑1 (MQ=25)


                                                                                 aaaTGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAg                 <  1:1200326/101‑1 (MQ=25)


                                                                                 aaaTGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAg                 <  1:3462558/101‑1 (MQ=25)


                                                                                  aaTGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgc                >  1:1021862/1‑101 (MQ=25)


                                                                                   aTGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcg               <  2:2379289/101‑1 (MQ=25)


                                                                                   aTGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcg               <  2:2147681/101‑1 (MQ=25)


                                                                                    tGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcgc              <  2:1248756/101‑1 (MQ=25)


                                                                                    tGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcgc              >  1:3420173/1‑101 (MQ=25)


                                                                                     ggAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGTGGCCG.GGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa             <  1:6916652/101‑1 (MQ=25)


                                                                                     ggAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa             >  2:4235965/1‑101 (MQ=25)


                                                                                     ggAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa             >  2:1340702/1‑101 (MQ=25)


                                                                                      gAAAAGTGCGCTCCGCT.GGGGCCGCCCTTTCATCCTGCCCCAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCAt            <  1:2076620/101‑1 (MQ=25)


                                                                                      gAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCAt            <  2:3366882/101‑1 (MQ=25)


                                                                                      gAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCAt            >  1:5789234/1‑101 (MQ=25)


                                                                                       aaaaGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATg           <  2:813604/101‑1 (MQ=25)


                                                                                       aaaaGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATg           <  1:5906091/101‑1 (MQ=25)


                                                                                        aaaGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATgg          >  1:4111674/1‑101 (MQ=25)


                                                                                          aGGGCGCTCCGCTGGGGG.CTCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgcg        <  1:2669381/101‑1 (MQ=25)


                                                                                          aGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgcg        <  1:3001846/101‑1 (MQ=25)


                                                                                            ggcgctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGct      >  2:6165062/6‑101 (MQ=25)


                                                                                             gcgctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGctt     >  1:6744959/5‑101 (MQ=25)


                                                                                              cgctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttc    <  1:3614397/98‑1 (MQ=25)


                                                                                              cgctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttc    <  1:1465274/98‑1 (MQ=25)


                                                                                              cgctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttc    >  1:5970704/4‑101 (MQ=25)


                                                                                               gctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttct   >  1:2538080/3‑101 (MQ=37)


                                                                                                ctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttctt  <  1:3230576/100‑1 (MQ=37)


                                                                                                ctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCCGGGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttctt  >  2:6970402/2‑101 (MQ=37)


CGATCGAGTGACACGGGCGC.A.GGGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGG.CGCCCTTTCATCTTGCCACAAATATCCCGG.GGTCC.GGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGCTTCTT  >  NC_014034/1843328‑1843518

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG