BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,590,199	+C	100%	intergenic (‑98/‑158)	glnE ← / → RCAP_rcc02415	glutamate‑ammonia‑ligase adenylyltransferase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,590,199	1	.→C	100.0%	17.7	137	intergenic (‑98/‑158)	glnE/RCAP_rcc02415	glutamate‑ammonia‑ligase adenylyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand): new base (129/0): ref base (2/6): total (131/6)
Fisher's exact test strand distribution p-value = 3.41e-09
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.52e-01

Only 100 of 173 total aligned reads displayed.


CATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CG.C.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTGACCCG  >  NC_014034/2590099‑2590294


cATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAg.cg                                                                                                       >  1:6576555/1‑101 (MQ=255)


cATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAg.cg                                                                                                       >  2:6299804/1‑101 (MQ=255)


 aTGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAg.cg.c                                                                                                     >  2:3535935/1‑101 (MQ=255)


    gcgcCAGCCTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CG.c.ccc                                                                                                 <  2:4591548/101‑1 (MQ=255)


    gccccAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CG.c.ccc                                                                                                 <  1:3175279/98‑1 (MQ=255)


     ccccAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CG.c.ccc.c                                                                                               <  1:4419634/99‑1 (MQ=255)


      gcCAGCCTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CG.c.ccc.cc                                                                                              <  2:5749936/101‑2 (MQ=255)


      cccAGCCTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CG.c.ccc.cc                                                                                              <  1:2188454/100‑2 (MQ=255)


        cAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CG.c.ccc.ccgc                                                                                            >  1:1270940/1‑98 (MQ=255)


          gACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CG.c.ccc.ccgccg                                                                                          >  1:4210246/1‑96 (MQ=255)


          gACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CG.c.ccc.ccgccg                                                                                          >  1:1971565/1‑96 (MQ=255)


           aCTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCgg                                                                                       >  1:1003605/1‑101 (MQ=255)


            cTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCggg                                                                                      >  1:6186798/1‑101 (MQ=255)


             tatccgccgCAAGCCCCGGGTCAAGGCGCGCAAATCCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGGGCGGGGGG.CAG.CGCC.CCC.C..G.CCggag                                                                                     >  2:2250401/4‑99 (MQ=37)


                  gccgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCt                                                                                >  1:614468/1‑101 (MQ=255)


                  gccgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCt                                                                                >  1:2553619/1‑101 (MQ=255)


                  gccgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCt                                                                                >  1:3613298/1‑101 (MQ=255)


                   ccgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTc                                                                               >  2:2634579/1‑101 (MQ=255)


                   ccgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTc                                                                               >  2:3482545/1‑101 (MQ=255)


                     gcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCgg                                                                             >  1:2700958/1‑101 (MQ=255)


                      cAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGgc                                                                            >  2:1826900/1‑101 (MQ=255)


                      cAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGgc                                                                            >  1:6349326/1‑101 (MQ=255)


                      cAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGgc                                                                            >  2:3858565/1‑101 (MQ=255)


                       aaGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGCCATCTTGGCGCAAATATCCCGGGGGGGGCGGGGGG.CAG.CG.CTCCC.C..G.CCGGGTCGCCTCGgcg                                                                           >  2:1267098/1‑101 (MQ=38)


                         gccgggggTCAGGGCGGGCAACGCCCCCGCCCCGGTCGTCTGGGCGCGAATAGCCCGGGGGGGGGGGGGGG.CGGCCC.C.CCC.C..G.CCGGGTCGCCTCGGCGCa                                                                         <  1:2314584/96‑1 (MQ=255)


                           cccGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAg                                                                       >  1:3210077/1‑101 (MQ=255)


                            ccGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGc                                                                      >  2:5013140/1‑101 (MQ=255)


                                gTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAg                                                                  >  2:4047871/1‑101 (MQ=255)


                                gTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGACGCCTCGGCGCAAGCCgcg                                                                  >  2:5812720/1‑99 (MQ=255)


                                 tCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAgg                                                                 >  1:6455176/1‑101 (MQ=255)


                                  cAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGc                                                                >  1:6901456/1‑101 (MQ=255)


                                  cAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGc                                                                >  2:33387/1‑101 (MQ=255)


                                   aaGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCg                                                               >  1:4096747/1‑101 (MQ=255)


                                   aaGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCAGCGCAAGCCGAGGCg                                                               >  1:3091721/1‑101 (MQ=255)


                                    aGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGa                                                              >  2:1485232/1‑101 (MQ=255)


                                      gcgcgcAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGGGGCGaca                                                            >  2:3292253/1‑99 (MQ=255)


                                           cAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAAtctct                                                       >  2:4154282/1‑101 (MQ=255)


                                           cAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAAtctct                                                       >  2:3924464/1‑101 (MQ=255)


                                           cAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGCGGCGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGACGCGAAAtctct                                                       >  2:1436687/1‑101 (MQ=37)


                                            aaaTGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAAtctctc                                                      >  2:6567279/1‑101 (MQ=255)


                                              aTGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTccc                                                    >  2:3599829/1‑101 (MQ=255)


                                              aTGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGCGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTccc                                                    >  1:7008310/1‑101 (MQ=255)


                                                 ccccgccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGa                                                 >  1:5272593/1‑101 (MQ=255)


                                                 ccccgccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGa                                                 >  1:840925/1‑101 (MQ=255)


                                                  ccccccctTGTCATCTGGGCGCGAATA.CCCGGGGGGGGGGGGGGG.CCG.CC.C.CCC.CCGG.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGAc                                                <  1:3263788/101‑1 (MQ=21)


                                                   ccgccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGAcc                                               >  2:2772034/1‑101 (MQ=255)


                                                      ccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCt                                            >  2:4600496/1‑101 (MQ=255)


                                                       cccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCtt                                           >  2:2896843/1‑101 (MQ=255)


                                                         cTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCCCGGCCCAAGCCGCGGCGGAATCTCTCCCCGACCCCTTgc                                         >  2:741734/1‑101 (MQ=25)


                                                          tGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcg                                        >  1:4888113/1‑101 (MQ=255)


                                                          tGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcg                                        >  1:148382/1‑101 (MQ=255)


                                                          tGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGCCGCAAGCCGAGGCGAAGTCTCTCCCCGACCCCGTgcg                                        >  2:3265785/1‑101 (MQ=37)


                                                           gTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcgg                                       >  1:3886926/1‑101 (MQ=255)


                                                           gTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcgg                                       >  2:1190796/1‑101 (MQ=255)


                                                            tCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcggc                                      >  2:2081205/1‑101 (MQ=255)


                                                             cATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcggcg                                     >  1:307253/1‑101 (MQ=255)


                                                             cATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcggcg                                     >  2:4698723/1‑101 (MQ=255)


                                                              aTCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGgcgc                                    >  1:5770806/1‑101 (MQ=255)


                                                               tCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCa                                   >  1:3931158/1‑101 (MQ=255)


                                                               tCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCa                                   >  1:6523520/1‑101 (MQ=255)


                                                               tCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.AGCC.CCC.C..G.CCGGGTCGCCTCGGCGCACGCCGAGGCGCAATATCTCACCGACCCCTTGAGGCGCa                                   >  1:83426/1‑101 (MQ=25)


                                                               tCTGGGCGCGAATGCCCGGGGGGGGGCGGGGGG.CGGCCC.C.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCa                                   <  2:3160702/101‑1 (MQ=25)


                                                                  tGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCACTg                                >  1:32884/1‑101 (MQ=255)


                                                                  tGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATg                                >  1:4268583/1‑101 (MQ=255)


                                                                   ggCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGt                               >  2:6647117/1‑101 (MQ=255)


                                                                    gcgcGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTa                              >  2:2677489/1‑101 (MQ=255)


                                                                    gcgcGAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTa                              >  2:5123311/1‑101 (MQ=255)


                                                                       cgAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTaaaa                           >  1:6850529/1‑101 (MQ=255)


                                                                        gAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAGATCTCTCCCCGACCCCTTGCGGCGCAATGTaaaaa                          >  1:3373789/1‑101 (MQ=255)


                                                                        gAATATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTaaaaa                          >  1:4737490/1‑101 (MQ=255)


                                                                         aaTATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAAt                         >  1:1921203/1‑101 (MQ=255)


                                                                         aaTATCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAAt                         >  1:1591787/1‑101 (MQ=255)


                                                                          atatCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATc                        >  1:7076923/1‑101 (MQ=255)


                                                                          atatCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATc                        >  2:4265070/1‑101 (MQ=255)


                                                                          atatCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGGCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGCAAAAATc                        >  2:2988024/1‑101 (MQ=38)


                                                                          aagccccGGGGGGGGGGGGGGGCCCGCCC.C.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAAt                         <  2:1013652/97‑1 (MQ=25)


                                                                           tatCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCt                       >  2:3669020/1‑101 (MQ=255)


                                                                           tatCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCCTGCGGCGCAATGTAAAAATCt                       >  1:2405902/1‑101 (MQ=255)


                                                                            tgcccGGGGGGGGCGGGGGGCCGG.CC.C.CCC.C..GACCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCt                       <  2:5272327/99‑1 (MQ=37)


                                                                            atCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCtt                      >  2:4870134/1‑101 (MQ=255)


                                                                            atCCCGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCCTGCGGCGCAATGTAAAAATCtt                      >  2:5024854/1‑101 (MQ=255)


                                                                              cggggggggGGCGGGCCG.CCC.CG.C.CCC.CG.G.CCGGGTCGCCTGGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTc                    <  2:7062119/98‑1 (MQ=37)


                                                                              cccGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTc                    >  1:5365734/1‑101 (MQ=255)


                                                                              cccGGGGGGGGGGGGGGGCCGG.CC.C.CCC.CG.G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCttt                     <  2:5269482/101‑1 (MQ=37)


                                                                               cgggggggGGGGGGGGG.CCGCCC.C.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTca                   <  2:3142684/99‑1 (MQ=37)


                                                                               ccGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTca                   >  1:754672/1‑101 (MQ=255)


                                                                               ccGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTca                   >  1:4726882/1‑101 (MQ=255)


                                                                                cGGGGGGTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTcac                  >  2:5450995/1‑101 (MQ=255)


                                                                                 ggggggTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTcaca                 >  1:1037765/1‑101 (MQ=255)


                                                                                  gggtgTGGGGGGGC.CGC.CC.C.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATc               <  2:1174797/101‑1 (MQ=37)


                                                                                  gggggTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACAt                >  2:1683430/1‑101 (MQ=255)


                                                                                   ggggTGCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATc               >  2:5708986/1‑101 (MQ=255)


                                                                                    gggggggggggg.CAGCCC.C.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATcc              <  2:1789813/95‑1 (MQ=38)


                                                                                     ggcgCGGGGCG.CCG.CC.C.CCCGC..G.CGGGGTCGCCGCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATccc             <  2:1617816/98‑1 (MQ=37)


                                                                                        gCGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCctc          >  1:4381435/1‑101 (MQ=255)


                                                                                         ggggggg.CCG.CCCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCctct         <  2:6454846/100‑1 (MQ=38)


                                                                                         cGGGGGG.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGGGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCctct         >  1:5013026/1‑101 (MQ=255)


                                                                                           ggggg.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGCCCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTg       >  1:514038/1‑101 (MQ=255)


                                                                                           ggggg.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCtta       >  1:3458613/1‑100 (MQ=255)


                                                                                               g.CAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTgaccc   >  1:6215912/1‑101 (MQ=255)


CATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGG.CAG.CG.C.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTGACCCG  >  NC_014034/2590099‑2590294

Base quality scores:


ATCG

< 3 ≤


ATCG

< 16 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG