Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,648,333 +G 99.0% intergenic (‑83/‑73) serB ← / → serC phosphoserine phosphatase SerB/phosphoserine aminotransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,648,3331.→G99.0% 2.1 0.0 ‑1.8402intergenic (‑83/‑73)serB/serCphosphoserine phosphatase SerB/phosphoserine aminotransferase
Reads supporting (aligned to +/- strand):  new base (204/194):  ref base (2/2):  total (206/196)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.47e-02

Only 100 of 438 total aligned reads displayed.

AAGCGTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.A.GGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGCCCCGGCCAA  >  NC_014034/3648233‑3648428
                                                                                                       |                                                                                               
gaGCGGGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.Cag.a                                                                                                  <  2:6933361/100‑1 (MQ=255)
aaGCGTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.Cag.a                                                                                                  <  1:2666998/101‑1 (MQ=255)
 aGCGTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.Cag.a.g                                                                                                >  2:6630694/1‑101 (MQ=255)
   cGTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.A.ggg                                                                                              >  1:641143/1‑101 (MQ=255)
    gTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.A.gggg                                                                                             >  1:1230341/1‑100 (MQ=255)
    gTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.A.gggg                                                                                             >  2:1593291/1‑100 (MQ=255)
     tGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.A.ggggc                                                                                            >  1:5328085/1‑99 (MQ=255)
      ggCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.A.ggggca                                                                                           >  1:5026897/1‑98 (MQ=255)
         gATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAg                                                                                         >  2:1839632/1‑101 (MQ=255)
          aTGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGc                                                                                        >  2:231297/1‑101 (MQ=255)
           tGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGcc                                                                                       >  1:3943483/1‑101 (MQ=255)
            gAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCt                                                                                      >  2:4922883/1‑101 (MQ=255)
            gAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCt                                                                                      <  2:3308928/101‑1 (MQ=255)
               cATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTa                                                                                   <  2:564599/101‑1 (MQ=255)
               cATGGATCAGGGGTCCCGGGGCTGGAGGGACGTCGCTTTTGATAACCGATCCGGCGCTTTAGCGTGAATTTCCATCTAGTGCACAA.G.GTGCAAAGCCTgca                                                                                   >  1:7076360/1‑99 (MQ=255)
                 tGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTagc                                                                                 <  1:5961920/101‑1 (MQ=255)
                 tGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTagc                                                                                 <  1:5128471/101‑1 (MQ=255)
                  ggATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCTATCTTGTGC.CAG.AGGGGCAAAGCCTGTagca                                                                                <  2:3898302/101‑1 (MQ=255)
                  ggATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTagca                                                                                <  2:1241054/101‑1 (MQ=255)
                    aTCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTagcagc                                                                              >  1:6611155/1‑101 (MQ=255)
                      cAGGGGGCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGa                                                                            <  2:6760491/101‑1 (MQ=255)
                        ggggTCCCGGGGCGGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGAGC.CAG.AGGGGCAAAGCCTGTAGCAGCGAcg                                                                          <  2:3554736/101‑1 (MQ=255)
                          ggTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGAcggc                                                                        <  2:27088/101‑1 (MQ=255)
                          ggTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGAcggc                                                                        <  1:1682589/101‑1 (MQ=255)
                           gTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGAcggcg                                                                       >  1:6702870/1‑101 (MQ=255)
                             gcgggggCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCggg                                                                     <  1:5691798/98‑1 (MQ=255)
                             cccGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCggg                                                                     >  1:404199/1‑101 (MQ=255)
                              ccGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGa                                                                    <  1:5463215/101‑1 (MQ=255)
                               cGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGac                                                                   <  1:4408563/101‑1 (MQ=255)
                               cGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGac                                                                   <  1:2251200/101‑1 (MQ=255)
                                ggggCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAGTG.GGGCAAAGCCTGTAGCAGCGACGGCGGGaca                                                                  >  1:3638071/1‑101 (MQ=255)
                                ggggCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGaca                                                                  <  2:2831582/101‑1 (MQ=255)
                                   gCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACAccc                                                               <  1:5226383/101‑1 (MQ=255)
                                    cTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCt                                                              >  2:7023879/1‑101 (MQ=255)
                                    cTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCt                                                              <  1:1113588/101‑1 (MQ=255)
                                     tGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGGCACCCtt                                                             >  1:6493182/1‑101 (MQ=255)
                                     tGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCtt                                                             >  2:763243/1‑101 (MQ=255)
                                     tGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCtt                                                             <  2:4489293/101‑1 (MQ=255)
                                     tGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCtt                                                             <  2:4274877/101‑1 (MQ=255)
                                      ggAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTc                                                            >  2:2467499/1‑101 (MQ=255)
                                      ggAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTc                                                            >  2:1320326/1‑101 (MQ=255)
                                      ggAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTc                                                            <  2:4455159/101‑1 (MQ=255)
                                      ggAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTc                                                            >  1:1938100/1‑101 (MQ=255)
                                       gAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTcc                                                           >  2:4105668/1‑101 (MQ=255)
                                        aGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGAACCCCTTtcc                                                          >  2:5999475/1‑101 (MQ=255)
                                          ggATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCa                                                        <  2:6283724/101‑1 (MQ=255)
                                          ggATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCa                                                        <  1:3005652/101‑1 (MQ=255)
                                            aTGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAAc                                                      <  1:1500737/101‑1 (MQ=255)
                                             tGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACg                                                     >  1:974240/1‑101 (MQ=255)
                                                cGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAg                                                  >  2:6098049/1‑101 (MQ=255)
                                                   ttttGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGc                                               >  2:5875247/1‑101 (MQ=255)
                                                    tttGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCg                                              <  2:4060745/101‑1 (MQ=255)
                                                     ttGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGt                                             >  1:1053669/1‑101 (MQ=255)
                                                     ttGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGt                                             >  1:4284757/1‑101 (MQ=255)
                                                       gATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGttt                                           <  2:4641742/101‑1 (MQ=255)
                                                       gATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGttt                                           <  1:4915499/101‑1 (MQ=255)
                                                        atatCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTa                                          <  2:581318/101‑1 (MQ=255)
                                                        atatCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTa                                          <  1:1593352/101‑1 (MQ=255)
                                                          atCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATc                                        <  1:6317775/101‑1 (MQ=255)
                                                           tCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCt                                       >  2:5257812/1‑101 (MQ=255)
                                                            ccGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTg                                      <  2:880968/101‑1 (MQ=255)
                                                             cGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTgg                                     <  2:5445222/101‑1 (MQ=255)
                                                              gATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGa                                    >  2:6215015/1‑101 (MQ=255)
                                                               aTGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGaa                                   >  1:6924245/1‑101 (MQ=255)
                                                               aTGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGaa                                   <  1:2863707/101‑1 (MQ=255)
                                                                tGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAg                                  <  2:1971271/101‑1 (MQ=255)
                                                                 gcggcgCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAgg                                 >  2:1664762/1‑101 (MQ=255)
                                                                   ggcgCTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGaa                               >  2:4211722/1‑100 (MQ=255)
                                                                     cgcTTTAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAg                             >  1:6804980/1‑101 (MQ=255)
                                                                        tttAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGGCGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGa                          >  1:4209101/1‑101 (MQ=255)
                                                                        tttAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGa                          <  1:4484721/101‑1 (MQ=255)
                                                                          tAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACa                        <  1:357628/101‑1 (MQ=255)
                                                                          tAGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACa                        <  2:5320341/101‑1 (MQ=255)
                                                                           aGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCTACGAAGGGCGTTTATCTGGAAGGATAGCGACAt                       <  1:3716186/101‑1 (MQ=255)
                                                                           aGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACAt                       >  1:2053851/1‑101 (MQ=255)
                                                                           aGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACAt                       >  1:6060759/1‑101 (MQ=255)
                                                                           aGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACAt                       <  1:1288009/101‑1 (MQ=255)
                                                                           aGCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTAATCTGGAAGGATAGCGACAt                       >  1:5601612/1‑101 (MQ=255)
                                                                            gCGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATg                      <  2:6490802/101‑1 (MQ=255)
                                                                             cGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGa                     <  1:2343188/101‑1 (MQ=255)
                                                                             cGTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGa                     >  1:3210692/1‑101 (MQ=255)
                                                                              gTGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGAc                    <  2:3057020/101‑1 (MQ=255)
                                                                               tGATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACg                   >  1:3285757/1‑101 (MQ=255)
                                                                                gATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGa                  <  1:1796638/101‑1 (MQ=255)
                                                                                gATTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGa                  >  2:1435822/1‑101 (MQ=255)
                                                                                 aTTTTCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGaa                 >  1:3356396/1‑101 (MQ=255)
                                                                                  ttttCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAAt                <  1:2929356/101‑1 (MQ=255)
                                                                                  ttttCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAAt                <  1:1321602/101‑1 (MQ=255)
                                                                                  ttttCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAAt                <  1:3803198/101‑1 (MQ=255)
                                                                                   tttCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATa               <  2:1095781/101‑1 (MQ=255)
                                                                                   tttCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATa               >  1:253266/1‑101 (MQ=255)
                                                                                   gttCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATa               <  2:266654/100‑1 (MQ=255)
                                                                                    ttCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATAc              <  1:4649619/101‑1 (MQ=255)
                                                                                    ttCCATCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATAc              >  1:1024366/1‑101 (MQ=255)
                                                                                        aTCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGcc          <  2:4820715/101‑1 (MQ=255)
                                                                                         tCTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGccc         <  2:5122595/101‑1 (MQ=255)
                                                                                          cTTGTGC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGcccc        >  2:2067707/1‑101 (MQ=255)
                                                                                             gtgC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGCCCCGGc     <  1:47586/101‑1 (MQ=255)
                                                                                             gtgC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGCCCCGGc     >  1:834450/1‑101 (MQ=255)
                                                                                              tgC.CAG.AGGGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGCCCCGGcc    <  1:4109240/101‑1 (MQ=255)
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AAGCGTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGC.CAG.A.GGGCAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGCCCCGGCCAA  >  NC_014034/3648233‑3648428

Base quality scores:  ATCG  < 3 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG