BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	904,934	+C	87.8%	intergenic (+38/‑158)	RCAP_rcc00821 → / → murB	hypothetical protein/UDP‑N‑acetylmuramate dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	904,934	2	.→C	87.8%	75.8 ‑2.4 ‑0.3	205	intergenic (+38/‑158)	RCAP_rcc00821/murB	hypothetical protein/UDP‑N‑acetylmuramate dehydrogenase
Reads supporting (aligned to +/- strand): new base (92/88): ref base (8/17): total (100/105)
Fisher's exact test strand distribution p-value = 8.88e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.94e-01

Only 100 of 224 total aligned reads displayed.


GGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTTG.G.CT..GC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCGGACG  >  NC_014034/904834‑905025


gggCTGAGCGTGCTGCGCTGGCCGGTGCGGGGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGGGGTGGCCCCCTC.TTTTG.G.CT..g                                                                                              >  1:3993445/1‑101 (MQ=255)


gggCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..g                                                                                              >  1:4303170/1‑101 (MQ=255)


gggCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..g                                                                                              >  1:4154306/1‑101 (MQ=255)


     gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTGTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgcca                                                                                         <  1:6655536/101‑5 (MQ=37)


     gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGGGCTGCCCCCCTC.TTTTG.G.CT..Gcgcca                                                                                         >  1:1924330/1‑97 (MQ=38)


     gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgcca                                                                                         >  2:7105150/1‑97 (MQ=255)


     gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgcca                                                                                         <  2:2514375/101‑5 (MQ=255)


     gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgcca                                                                                         <  1:5545952/101‑5 (MQ=255)


     gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCC.CCTTTTG.G.CT..Gcgcca                                                                                         >  2:4034612/1‑97 (MQ=38)


         gTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaat                                                                                     <  2:4825610/101‑9 (MQ=37)


         gTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaat                                                                                     >  2:2245973/1‑93 (MQ=37)


         gTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaat                                                                                     >  1:4391708/1‑93 (MQ=37)


         gTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaat                                                                                     >  1:2979184/1‑93 (MQ=37)


         gTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaat                                                                                     >  1:100660/1‑93 (MQ=37)


          tgctgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CT..Gcgccaaaatt                                                                                    >  2:4602732/1‑92 (MQ=25)


            ctgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCa                                                                                  <  2:6474232/101‑1 (MQ=25)


               cgcTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCAccc                                                                               <  1:1542579/101‑1 (MQ=25)


               cgcTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCAccc                                                                               <  1:6909551/101‑1 (MQ=25)


                gcTGGCTGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.CAAATTCAcccc                                                                              >  1:5516672/1‑101 (MQ=21)


                gcTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCAcccc                                                                              <  2:4442851/101‑1 (MQ=25)


                gcTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCAcccc                                                                              <  1:4162323/101‑1 (MQ=25)


                  tGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCAcccccc                                                                            <  2:5680620/101‑1 (MQ=21)


                   ggCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGGTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCg                                                                           >  1:3977891/1‑101 (MQ=21)


                   ggCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCg                                                                           >  1:5965542/1‑101 (MQ=21)


                   ggCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCg                                                                           >  2:1021576/1‑101 (MQ=21)


                     cgcgTGCGGCGGCTCTGTGCCGCGATGCACCGCCGCGACTGAGGGCGGCGCAGGGGGGCGCCGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGc                                                                         <  1:3307565/98‑1 (MQ=18)


                      cGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCg                                                                        >  2:2056464/1‑101 (MQ=21)


                       ggTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTTACCCCCCCGcct                                                                       >  2:577040/1‑99 (MQ=21)


                        gTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTa                                                                      >  1:1085318/1‑101 (MQ=21)


                         tGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTAt                                                                     <  2:6223749/101‑1 (MQ=21)


                          gcggcgGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTAtt                                                                    <  2:2424785/101‑1 (MQ=21)


                          gcggcgGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGGAtt                                                                    >  1:3396738/1‑101 (MQ=21)


                           gcgcgccTCTTTGCCGCGCTGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTAttt                                                                   <  2:6922718/99‑1 (MQ=18)


                            ggcggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTGG.NTGC..GC.C.AAAATTCAACCCCCGGCGTATTTg                                                                  >  1:1248329/1‑101 (MQ=21)


                            ggcggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTg                                                                  >  2:6569537/1‑101 (MQ=21)


                             gcggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGc                                                                 >  2:683868/1‑101 (MQ=21)


                              cggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGGCTTTGCa                                                                >  1:2575927/1‑101 (MQ=18)


                                  tctTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.CAAATTCACCCCCCGGCGGATTTGCACCAg                                                            >  2:49534/1‑101 (MQ=21)


                                  tctTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCAcccg                                                            >  1:1562277/1‑99 (MQ=18)


                                   ctctGCCGCGATGCTCGGGCGCGGCTGAGGGCGGGGCAGGGGCGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAgg                                                           <  2:5619983/98‑1 (MQ=17)


                                   ctTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.NAAATTCACCCCCCGGCGTATTTGCACCAgg                                                           >  1:2933035/1‑101 (MQ=21)


                                    tttGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGa                                                          <  1:1825527/101‑1 (MQ=21)


                                    tttGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGa                                                          >  2:4267925/1‑101 (MQ=21)


                                    tttGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGa                                                          >  2:4110532/1‑101 (MQ=21)


                                    tttGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGa                                                          <  1:5003993/101‑1 (MQ=21)


                                     ttGCCGGGATGCACCGGCGCCACTGAGGGCTGGGTGGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCCCCCCCCGGCGTATTTGCACCACGaa                                                         <  1:6828886/101‑1 (MQ=17)


                                     ttGCCGCGATGCACCGGCACGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCTCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGaa                                                         <  2:1240396/101‑1 (MQ=21)


                                      tGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGaaa                                                        >  2:1064592/1‑101 (MQ=21)


                                        ccGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGaaaaa                                                      >  2:5452588/1‑101 (MQ=21)


                                        ccGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGaaaaa                                                      <  2:3585706/101‑1 (MQ=21)


                                          gcgATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGc                                                    >  2:2847289/1‑101 (MQ=21)


                                            gATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGccc                                                  >  1:6087587/1‑101 (MQ=21)


                                                   cGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGttt                                           <  1:6220331/101‑1 (MQ=21)


                                                   cGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGACCGGCGttt                                           >  2:3254240/1‑101 (MQ=18)


                                                    ggCGCGACTGAGGGCGGGGCAGGGGGGCGGTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtttt                                          >  1:3903357/1‑101 (MQ=21)


                                                    ggCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtttt                                          >  2:1335760/1‑101 (MQ=21)


                                                     gggcgACTGAGGGCGGGGCAGGGGGGCGCTGCCTCCCTC.TTTTG.G.CTGCGC.C.AAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGttttt                                         <  1:113890/99‑1 (MQ=25)


                                                     gcgcGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGttttt                                         <  1:3659260/101‑1 (MQ=21)


                                                      cgcgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTc                                        >  1:1411551/1‑101 (MQ=21)


                                                        cgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCt                                      >  2:3018131/1‑101 (MQ=21)


                                                         gACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTg                                     >  2:5959744/1‑101 (MQ=21)


                                                         gACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTg                                     <  1:6475807/101‑1 (MQ=21)


                                                             gAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCg                                 <  1:4670348/101‑1 (MQ=21)


                                                                 gCGGGGCCGGGGGGCGCTGCCCCCCTCTTTTAGCT.GC..GC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAAt                             <  2:6140629/101‑1 (MQ=21)


                                                                 gCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAAt                             <  2:5370921/101‑1 (MQ=21)


                                                                  cGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATa                            >  1:753298/1‑101 (MQ=21)


                                                                   ggggCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATAc                           >  1:3258497/1‑101 (MQ=21)


                                                                    gggCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACg                          >  2:4931187/1‑101 (MQ=21)


                                                                     ggCAGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGc                         >  2:398822/1‑101 (MQ=21)


                                                                      gCAGGGGGGCGCCGCCCCCCCCTTTTGG.GTGC..GC.C.AAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGcc                        <  2:6051194/101‑1 (MQ=37)


                                                                        aGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgg                      >  2:5356451/1‑101 (MQ=25)


                                                                        aGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgg                      >  1:663199/1‑101 (MQ=25)


                                                                        aGGGGGGCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgg                      >  1:2857747/1‑101 (MQ=25)


                                                                          gggggCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCGCGGCGTATGTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCGgggg                    <  2:2591441/101‑1 (MQ=21)


                                                                          gggggCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggg                    >  1:5686377/1‑101 (MQ=25)


                                                                            gggCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAGATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggggg                  <  1:2736898/101‑1 (MQ=25)


                                                                            gggCGCTGCCCCCCTC.GTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggggg                  <  2:484087/101‑1 (MQ=25)


                                                                             ggCGCTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGa                 >  1:4749875/1‑101 (MQ=25)


                                                                              gcgcTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAg                <  1:5356723/101‑1 (MQ=25)


                                                                               cgcTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGt               <  1:1606149/101‑1 (MQ=25)


                                                                               cgcTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGt               <  1:7105524/101‑1 (MQ=25)


                                                                               cgcTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGt               <  2:1924258/101‑1 (MQ=25)


                                                                                gcTGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTc              >  2:4510434/1‑101 (MQ=37)


                                                                                 cTGCCCCCGTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc             <  2:467877/101‑1 (MQ=25)


                                                                                  tGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCg            >  2:3156823/1‑101 (MQ=37)


                                                                                  tGCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCg            <  1:1665263/101‑1 (MQ=37)


                                                                                   gCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGc           >  1:2355670/1‑101 (MQ=37)


                                                                                   gCCCCCCTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGGCCGc           >  2:4736382/1‑101 (MQ=25)


                                                                                    ccccccTC.TTTTG.G.CTGCGC.C.AAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCt          <  2:6810260/101‑1 (MQ=25)


                                                                                    ccccccTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCt          <  2:1628784/101‑1 (MQ=37)


                                                                                    ccccccTC.TTTTG.G.CT..GCGCGAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCt          <  1:4369364/101‑1 (MQ=25)


                                                                                     cccccTC.TTTTG.G.CTGCGC.C.AAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCtt         <  1:3416459/101‑1 (MQ=25)


                                                                                     cccccTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCtt         <  2:6855193/101‑1 (MQ=37)


                                                                                       cccTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGTCCGAAATACGCGGGGGGGAGCCCCCTTGc       >  1:6698488/1‑101 (MQ=25)


                                                                                       cccTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGc       <  2:1114356/101‑1 (MQ=37)


                                                                                       cccTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGc       >  1:5999311/1‑101 (MQ=37)


                                                                                       cccTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGc       <  2:6585511/101‑1 (MQ=37)


                                                                                       cccTC.TTTTG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGc       <  2:2145426/101‑1 (MQ=37)


                                                                                           tcttttG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCGGa    <  2:4302948/99‑1 (MQ=37)


                                                                                            cttttG.G.CTGCGC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCGGAc   >  1:5217670/2‑101 (MQ=255)


GGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTTG.G.CT..GC.C.AAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCGGACG  >  NC_014034/904834‑905025

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG