Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,398,0351.→C 0.7% 0.5 0.0 ‑0.4414intergenic (+339/+1559)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (1/2):  ref base (204/207):  total (205/209)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.96e-01

Only 100 of 444 total aligned reads displayed.

TCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATTCCGC  >  NC_014034/1397935‑1398135
                                                                                                       |                                                                                                    
tctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCa                                                                                                       <  1:2141597/101‑1 (MQ=255)
 ctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCa.a                                                                                                     <  2:2407438/101‑1 (MQ=255)
     gggaggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCg                                                                                                 <  2:3236675/101‑1 (MQ=255)
        aggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTc                                                                                              >  2:6975306/1‑101 (MQ=255)
           ctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccc                                                                                           <  2:2729769/101‑1 (MQ=255)
           ctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccc                                                                                           <  2:4360832/101‑1 (MQ=255)
            tagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTccccc                                                                                          >  1:6437922/1‑101 (MQ=255)
              gggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTccccccc                                                                                        >  2:135248/1‑100 (MQ=255)
               ggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCcgc                                                                                       >  1:4047740/1‑101 (MQ=255)
               ggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCcgc                                                                                       <  1:2366142/101‑1 (MQ=255)
                 aggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCgg                                                                                     >  1:1746805/1‑101 (MQ=255)
                  ggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCggg                                                                                    >  1:3603620/1‑101 (MQ=255)
                   gcttCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGACACCGCCGGGCAACAAGTCCGCCAAGAAGGCG..AGCA.AGTCACTCTCCCCGCggac                                                                                   >  2:258344/1‑99 (MQ=25)
                   gctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCgggg                                                                                   >  1:5432183/1‑101 (MQ=255)
                   gctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCgggg                                                                                   >  1:6137303/1‑101 (MQ=255)
                    ctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCggggg                                                                                  >  2:6187268/1‑101 (MQ=255)
                     taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGc                                                                                 >  1:1407642/1‑101 (MQ=255)
                       cGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGt                                                                               >  2:3936880/1‑101 (MQ=255)
                       cGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGt                                                                               <  1:5655982/101‑1 (MQ=255)
                       cGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGt                                                                               >  2:2331354/1‑101 (MQ=255)
                        gCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTg                                                                              >  2:5344512/1‑101 (MQ=255)
                        gCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTg                                                                              >  1:6081682/1‑101 (MQ=255)
                          aCCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGa                                                                            >  2:4817563/1‑101 (MQ=255)
                            cTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAAAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                          >  1:5104088/1‑101 (MQ=255)
                             ttCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                         >  2:3548883/1‑101 (MQ=255)
                             ttCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                         <  2:6649420/101‑1 (MQ=255)
                             ttCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                         >  2:5150474/1‑101 (MQ=255)
                             ccccaaaGTCACACCCCCCGCGGAGGCGTGTATAAAATCCGCGGCGCCAAAAGCCAGCCAAGAACGGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGACCg                                                                         <  2:2095298/97‑1 (MQ=16)
                              tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.CGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                          <  2:3076800/95‑1 (MQ=2)
                              tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.CGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                          <  1:5901071/95‑1 (MQ=2)
                              tCAGAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCTCCCGCGGGGGCGTGGATCGt                                                                        >  1:5595387/1‑101 (MQ=255)
                              tCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGt                                                                        >  1:6986578/1‑101 (MQ=255)
                                   aGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCg                                                                   <  1:6578700/101‑1 (MQ=255)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCggg                                                                 >  2:4335885/1‑100 (MQ=255)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTg                                                                >  2:1933749/1‑101 (MQ=255)
                                           cccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCg                                                           <  1:333622/101‑1 (MQ=255)
                                               cgcgGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGccc                                                       <  1:3503197/101‑1 (MQ=255)
                                                gcgGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCg                                                      <  2:849240/101‑1 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGa                                                     >  2:67276/1‑101 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGa                                                     >  1:5237665/1‑101 (MQ=255)
                                                  gggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGAt                                                    >  1:6710885/1‑101 (MQ=255)
                                                  gggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGAt                                                    >  2:4698507/1‑101 (MQ=255)
                                                  gggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGAt                                                    >  2:3798566/1‑101 (MQ=255)
                                                  gggggCGTAGCTGGAAACCGCCGAGCAAAAAGCCCGCCAAGAAGCCG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATAGTAAGCGGTGCATCGGCCCGAt                                                    <  2:5505505/101‑1 (MQ=38)
                                                     ggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaaa                                                 <  1:917987/101‑1 (MQ=255)
                                                      gggTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAc                                                <  1:1503319/99‑1 (MQ=255)
                                                        gTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAccc                                              >  1:1938364/1‑101 (MQ=255)
                                                            aTCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAt                                          <  1:4817806/101‑1 (MQ=255)
                                                            aTCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAt                                          <  1:4948461/101‑1 (MQ=255)
                                                             tCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtt                                         <  2:1646624/101‑1 (MQ=255)
                                                               gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtttt                                       >  2:4170717/1‑101 (MQ=255)
                                                               gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtttt                                       <  1:4715500/101‑1 (MQ=255)
                                                                 aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg                                     >  2:2191583/1‑101 (MQ=255)
                                                                 aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg                                     <  1:125646/101‑1 (MQ=255)
                                                                   ccgccgCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGc                                   <  1:6809439/101‑1 (MQ=255)
                                                                     gccgcGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGccc                                 <  2:368314/101‑1 (MQ=255)
                                                                      ccgcGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGcccc                                >  1:2255294/1‑101 (MQ=255)
                                                                        gcgcAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTg                              >  2:598955/1‑101 (MQ=255)
                                                                        gcgcAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTg                              <  2:6986205/101‑1 (MQ=255)
                                                                        ccgcAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTg                              <  2:5431911/100‑1 (MQ=255)
                                                                         ggcAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGa                             <  2:1724569/100‑1 (MQ=255)
                                                                         cgcAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCtcg                             <  2:2613014/101‑3 (MQ=255)
                                                                         cgcAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGa                             <  1:2598374/101‑1 (MQ=255)
                                                                          gcAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAg                            <  2:1407592/101‑1 (MQ=255)
                                                                           cAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgg                           >  2:633211/1‑101 (MQ=255)
                                                                           cAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgg                           <  1:1036538/101‑1 (MQ=255)
                                                                           cAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgg                           >  1:322900/1‑101 (MQ=255)
                                                                             aaaaGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgggg                         <  1:4276218/101‑1 (MQ=255)
                                                                              aaaGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGa                        >  1:3168944/1‑101 (MQ=255)
                                                                              aaaGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGa                        >  2:4540437/1‑101 (MQ=255)
                                                                               aaGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATTAACCCCGATTTTCGGCCCCTGAGGGGaa                       <  1:6282502/101‑1 (MQ=255)
                                                                                aGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGaaa                      <  2:4047564/101‑1 (MQ=255)
                                                                                  cccGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGa                    <  1:1144970/101‑1 (MQ=255)
                                                                                  cccGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGa                    <  1:5994994/101‑1 (MQ=255)
                                                                                    cGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGTGGAAAGAtt                  <  1:3864852/101‑1 (MQ=255)
                                                                                     gCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTc                 <  1:2083856/101‑1 (MQ=255)
                                                                                     gCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTc                 <  1:4983547/101‑1 (MQ=255)
                                                                                      ccAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCt                <  2:5237399/101‑1 (MQ=255)
                                                                                       cAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTTtt               >  1:2650064/1‑101 (MQ=255)
                                                                                       cAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCtt               <  1:182952/101‑1 (MQ=255)
                                                                                        aagaagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTg              >  1:2928102/1‑101 (MQ=255)
                                                                                         agaagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTgg             >  2:3367683/1‑101 (MQ=255)
                                                                                         agaagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTgg             >  2:1046111/1‑101 (MQ=255)
                                                                                          gaagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGc            <  1:460209/101‑1 (MQ=255)
                                                                                          gaagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGc            >  1:5187538/1‑101 (MQ=255)
                                                                                           aagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGca           >  2:4657107/1‑101 (MQ=255)
                                                                                           aagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGca           >  2:5668465/1‑101 (MQ=255)
                                                                                            agCCG..AGCC.AGGCGCGCCCCCCGCGGGTGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcac          <  1:709407/101‑1 (MQ=25)
                                                                                             gCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcaca         <  2:5629672/101‑1 (MQ=255)
                                                                                             gCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcaca         <  2:4951407/101‑1 (MQ=255)
                                                                                               gg..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATGGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAt       >  1:3721741/1‑101 (MQ=255)
                                                                                                g..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAtt      >  2:7106957/1‑101 (MQ=255)
                                                                                                g..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAtt      <  2:1822788/101‑1 (MQ=255)
                                                                                                   aGCA.AGTCGCGCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATTc     <  2:5787568/101‑1 (MQ=255)
                                                                                                    gCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATTcc    <  2:6852469/101‑1 (MQ=255)
                                                                                                     cA.AGTCGCTGCCCCCGCGGGGGCGTTGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATTCcg   <  2:2270381/101‑1 (MQ=255)
                                                                                                     cA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGCGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGCTTCTTGGCACAATTCcg   >  2:1240585/1‑101 (MQ=255)
                                                                                                      a.aGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATTCcgc  <  2:2927993/101‑1 (MQ=255)
                                                                                                      a.aGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATTCcgc  >  1:3060085/1‑101 (MQ=255)
                                                                                                      a.aGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATTCcgc  >  1:50394/1‑101 (MQ=255)
                                                                                                       |                                                                                                    
TCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATTCCGC  >  NC_014034/1397935‑1398135

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG