Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,950 A→G 21.3% M278V (ATC→GAC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9500A→G21.3% 288.5 ‑1.4 ‑0.1539M278V (ATC→GTC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (60/55):  ref base (194/230):  total (254/285)
Fisher's exact test strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.68e-01

Only 100 of 566 total aligned reads displayed.

GGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGG  >  NC_014034/1396850‑1397050
                                                                                                        |                                                                                                          
gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCa                                                                                                            >  1:1547404/1‑101 (MQ=255)
  gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                                         <  2:2743890/101‑1 (MQ=255)
      gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt                                                                                                     >  2:6047217/1‑101 (MQ=255)
       tGGATCTAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt.t                                                                                                   <  2:5943423/101‑1 (MQ=255)
        ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCCGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACA.GCCC.GCGCG.GC.CGCA.TCGGCtcc                                                                                                   >  2:2295174/1‑99 (MQ=255)
         gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.Tcc                                                                                                 <  2:6844051/101‑1 (MQ=255)
             gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                            >  2:5670544/1‑101 (MQ=255)
               aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                        <  2:146485/101‑1 (MQ=255)
                    gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.Gt                                                                                  <  1:1014352/101‑1 (MQ=255)
                     tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                                 >  1:5672399/1‑101 (MQ=255)
                     tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                                 <  2:2187321/101‑1 (MQ=255)
                           gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccc                                                                           >  1:1145102/1‑101 (MQ=255)
                           gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccc                                                                           <  1:1711357/101‑1 (MQ=255)
                              aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                        <  2:4378986/101‑1 (MQ=255)
                               gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccccc                                                                       >  2:1585898/1‑100 (MQ=255)
                               gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcg                                                                       <  1:468519/101‑1 (MQ=255)
                                   gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                                   >  2:3400314/1‑101 (MQ=255)
                                     gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                 <  2:2060205/101‑1 (MQ=255)
                                       ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                               >  2:4194044/1‑101 (MQ=255)
                                       ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                               >  2:3857666/1‑101 (MQ=255)
                                        gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCCC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCgg                                                              >  2:2152926/1‑100 (MQ=255)
                                          ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                             <  1:3973994/91‑1 (MQ=0)
                                           attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                           >  1:335455/11‑101 (MQ=0)
                                           attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                           <  2:3541586/91‑1 (MQ=0)
                                            tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                          >  1:4793131/1‑101 (MQ=255)
                                            cgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGG.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                           >  1:1994423/9‑101 (MQ=0)
                                             tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:6433906/93‑1 (MQ=2)
                                             tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         >  1:5774372/9‑101 (MQ=2)
                                             cctcGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  1:2742438/101‑1 (MQ=255)
                                             cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:1181542/101‑1 (MQ=255)
                                             ccccAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  1:266896/98‑1 (MQ=255)
                                              ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                        <  1:1904131/101‑1 (MQ=255)
                                              cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGG.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         >  2:2634859/7‑101 (MQ=0)
                                              caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         >  1:6233245/8‑101 (MQ=9)
                                                acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                       <  1:2400198/96‑1 (MQ=9)
                                                 ttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      <  1:5204680/98‑1 (MQ=2)
                                                  tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     >  1:4226807/3‑101 (MQ=2)
                                                  gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                    >  1:6151766/1‑101 (MQ=255)
                                                  ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                    >  1:2480413/4‑101 (MQ=2)
                                                  ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                    <  1:491709/98‑1 (MQ=2)
                                                  actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     >  1:4419317/4‑101 (MQ=9)
                                                   gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                    <  2:5003180/100‑1 (MQ=2)
                                                   gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                    <  1:1357235/100‑1 (MQ=2)
                                                   gcGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                    <  1:6676257/100‑1 (MQ=2)
                                                   cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                   <  2:2987923/99‑2 (MQ=2)
                                                    tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                   <  1:5313027/100‑2 (MQ=9)
                                                    ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                  >  2:4670868/2‑99 (MQ=2)
                                                    cggcGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                  <  1:3125862/98‑1 (MQ=255)
                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                 >  2:32791/1‑98 (MQ=2)
                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                  <  2:6528541/101‑3 (MQ=9)
                                                     gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                                  <  1:2852264/101‑3 (MQ=2)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                                 >  2:2877016/1‑98 (MQ=9)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                                 <  1:789161/101‑4 (MQ=9)
                                                      cccgcccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                <  2:533246/96‑1 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                  >  1:6984598/1‑100 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                               <  1:6895816/101‑6 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                 <  1:557487/101‑3 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                               >  1:125432/1‑96 (MQ=9)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGGGGATGGAAActcggc                                               >  1:2276543/1‑96 (MQ=9)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                              >  2:3088208/1‑95 (MQ=9)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaagatcgga                                             >  1:3202270/1‑93 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                            >  2:4083967/1‑101 (MQ=255)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                            >  1:6443168/1‑101 (MQ=255)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                             >  1:5472416/1‑94 (MQ=9)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                             <  1:1621520/101‑8 (MQ=9)
                                                           ccccccGGGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                            >  1:5982194/1‑93 (MQ=9)
                                                           ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                              >  2:6159428/1‑99 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                           >  2:893597/1‑92 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                           >  2:744687/1‑92 (MQ=2)
                                                            cccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgt                                           <  2:248111/101‑10 (MQ=2)
                                                             ccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaagat                                            >  1:7083844/1‑97 (MQ=2)
                                                             ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                         <  1:2153006/101‑1 (MQ=255)
                                                             ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                          >  2:2440326/1‑91 (MQ=2)
                                                               ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                          <  2:7025323/101‑7 (MQ=0)
                                                               ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                          <  2:553732/101‑7 (MQ=0)
                                                                cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                      <  1:4538050/101‑1 (MQ=255)
                                                                   gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                   >  1:6383036/1‑101 (MQ=255)
                                                                    ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                                  <  2:3656962/101‑1 (MQ=255)
                                                                     gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                 <  1:340206/101‑1 (MQ=255)
                                                                     gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                 <  2:6670335/101‑1 (MQ=255)
                                                                        cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                              <  2:1305590/101‑1 (MQ=255)
                                                                        cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                              <  2:1381927/101‑1 (MQ=255)
                                                                         gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCTCGTCGTTGGTCAGCCg                             >  2:5755535/1‑101 (MQ=255)
                                                                         gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                             >  1:3754556/1‑101 (MQ=255)
                                                                          tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                            >  1:2205117/1‑101 (MQ=255)
                                                                          tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                            >  2:5227036/1‑101 (MQ=255)
                                                                          tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                            >  2:1671299/1‑101 (MQ=255)
                                                                           ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCggc                           >  2:1040174/1‑100 (MQ=255)
                                                                           ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                           >  1:3051146/1‑101 (MQ=255)
                                                                             aTCGAACCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                         <  2:3175434/101‑1 (MQ=255)
                                                                                 aaaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCCC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAACCATCAGCTCGGCGTTGGTCAGCCCGACGATGt                     >  2:5800637/1‑101 (MQ=38)
                                                                                              gcg.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccc          >  2:1842478/1‑101 (MQ=255)
                                                                                                  gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc       >  2:487106/1‑101 (MQ=255)
                                                                                                   c.cGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcg      <  2:1983307/101‑1 (MQ=255)
                                                                                                   c.cGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcg      <  1:3847628/101‑1 (MQ=255)
                                                                                                     cGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgc     <  1:4186446/101‑1 (MQ=255)
                                                                                                      gCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg    >  1:5025948/1‑101 (MQ=255)
                                                                                                      gCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg    >  2:3976264/1‑101 (MQ=255)
                                                                                                        a.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGGCGGACGATGTCCGTCGCTCCCCCCGCggg  >  1:3562485/1‑101 (MQ=255)
                                                                                                        a.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg  >  1:95926/1‑101 (MQ=255)
                                                                                                        |                                                                                                          
GGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGG  >  NC_014034/1396850‑1397050

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG