BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,951	T→A	12.2%	I278N (ATC→GAC)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,951	0	T→A	12.2%	171.5 ‑2.3 ‑0.4	541	I278N (ATC→AAC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (34/27): ref base (185/238): total (257/284)
Fisher's exact test strand distribution p-value = 9.82e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.15e-01

Only 100 of 563 total aligned reads displayed.


GGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGG  >  NC_014034/1396851‑1397051


ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.t                                                                                                             <  1:4593213/101‑1 (MQ=255)


ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.t                                                                                                             >  2:2678799/1‑101 (MQ=255)


 gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                                            <  2:2474962/101‑1 (MQ=255)


     gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCt                                                                                                      >  2:1336815/1‑101 (MQ=255)


       ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.Tc                                                                                                   >  2:468507/1‑101 (MQ=255)


        gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.Tcc                                                                                                  <  2:6844051/101‑1 (MQ=255)


            gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAANNN.NNCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.Ct                                                                                             >  1:4253190/1‑101 (MQ=255)


            gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGc.cc                                                                                             >  2:6091773/1‑100 (MQ=255)


            gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.Ct                                                                                             <  1:1794405/101‑1 (MQ=255)


             aaaCCGGTCCAGAGATATACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTg                                                                                            >  2:2892474/1‑101 (MQ=255)


             aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTg                                                                                            >  2:2114988/1‑101 (MQ=255)


             aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTg                                                                                            >  1:2401046/1‑101 (MQ=255)


                 cGGTACAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTg                                                                                      >  2:1644415/1‑101 (MQ=255)


                  ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTg.g                                                                                    >  1:6241349/1‑101 (MQ=255)


                   gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.Gt                                                                                   <  1:1014352/101‑1 (MQ=255)


                    tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTc                                                                                  >  1:5672399/1‑101 (MQ=255)


                    tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTc                                                                                  <  2:6438024/101‑1 (MQ=255)


                    tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.TCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTc                                                                                  <  1:6409987/101‑1 (MQ=255)


                     ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCg                                                                                 >  2:4008051/1‑101 (MQ=255)


                      cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGGG.GTCGc                                                                                >  2:1502329/1‑101 (MQ=255)


                         agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTcc                                                                             <  2:541266/101‑1 (MQ=255)


                          gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccc                                                                            >  1:4431330/1‑101 (MQ=255)


                           aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTcccc                                                                           >  2:577398/1‑101 (MQ=255)


                           aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTcccc                                                                           >  2:5004847/1‑101 (MQ=255)


                            tagacccgccgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                          <  2:3794856/92‑1 (MQ=255)


                            tAGACCGCGCGGCCCTCCTCAGACGTCGGTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGTG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                          >  1:5068089/1‑101 (MQ=255)


                            tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                          <  2:2515112/101‑1 (MQ=255)


                            tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                          <  1:7091316/101‑1 (MQ=255)


                            tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                          <  1:4821969/101‑1 (MQ=255)


                             aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                         <  1:5474537/101‑1 (MQ=255)


                              gACCGCGCGGCCCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcg                                                                        <  2:5670868/101‑1 (MQ=255)


                                         ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                              >  1:5778906/11‑101 (MQ=0)


                                         cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CCG..CCGG.GCCGCTCCCCCCGCCGGGGCGTgg                                                             >  1:4962085/1‑101 (MQ=37)


                                          tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                             >  2:1404911/10‑101 (MQ=0)


                                          ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:3141917/91‑1 (MQ=9)


                                          ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                             >  1:6745831/11‑101 (MQ=9)


                                           ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           >  2:3867606/10‑101 (MQ=2)


                                            ccccAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:266896/98‑1 (MQ=255)


                                             cccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                            <  2:5954474/95‑1 (MQ=2)


                                             caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTGCCCCCGCGGGGGCGTGGATc                                                          <  2:1786359/94‑1 (MQ=9)


                                             caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  2:1101817/94‑1 (MQ=9)


                                              gccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  1:3750729/7‑101 (MQ=2)


                                              aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  1:6917777/7‑101 (MQ=9)


                                               cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  1:3176418/1‑101 (MQ=255)


                                               acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  2:4229678/6‑101 (MQ=9)


                                                 gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     >  1:6151766/1‑101 (MQ=255)


                                                  gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     <  1:3332509/100‑1 (MQ=2)


                                                   tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                    >  1:2216576/2‑100 (MQ=9)


                                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                   >  1:1266215/2‑99 (MQ=2)


                                                   cggcGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                   <  1:3125862/98‑1 (MQ=255)


                                                   cGTCGCTCCCCCCGCCGGGCGGTGGATCCAAACA.GCC..GCTCG.AC.CGCA.TCGCACCG.GGTCC.CCG..CGCC.GCCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                    <  2:2319531/101‑2 (MQ=2)


                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     >  2:3047057/1‑101 (MQ=2)


                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                  <  1:1934950/101‑4 (MQ=2)


                                                    gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     <  1:1169098/101‑1 (MQ=2)


                                                    gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                                   <  1:2852264/101‑3 (MQ=2)


                                                     tCGCTCCCCCCGCGGGGGCGTTGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccg                                                  <  1:2977699/101‑4 (MQ=2)


                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccc                                                  >  2:1022373/1‑98 (MQ=2)


                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                 <  1:6006965/101‑1 (MQ=255)


                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                 <  2:4592094/101‑1 (MQ=255)


                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                                 >  1:854282/1‑97 (MQ=2)


                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGACActacac                                                >  2:5560949/1‑96 (MQ=2)


                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                                 >  2:5863637/1‑97 (MQ=9)


                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                  >  1:7000007/1‑99 (MQ=2)


                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                >  2:1907558/1‑96 (MQ=2)


                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                               <  1:1645540/101‑7 (MQ=2)


                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGGGGATGGAAActcggc                                                >  1:2276543/1‑96 (MQ=9)


                                                         tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                             >  1:6443168/1‑101 (MQ=255)


                                                          ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                               <  1:1358053/101‑3 (MQ=2)


                                                          ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCGCCCGCGGGGGCGTGGATCGAAACATCAGcccc                                            >  1:4741080/1‑98 (MQ=255)


                                                          ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                            >  2:893597/1‑92 (MQ=2)


                                                          ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                            >  2:744687/1‑92 (MQ=2)


                                                          ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                            >  2:4139063/1‑92 (MQ=2)


                                                          ccccccGCGGGGGCGGGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacg                                             <  2:1994351/101‑9 (MQ=2)


                                                           cccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgt                                            <  2:3201685/101‑10 (MQ=2)


                                                            ccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgtc                                           <  2:5229637/101‑11 (MQ=2)


                                                              ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                           <  2:7025323/101‑7 (MQ=0)


                                                              ccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TGCGC.CTG..TGTG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAAATCAGCTcgtcgt                                        >  2:2843027/1‑101 (MQ=38)


                                                              ccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                                        <  2:2232829/101‑1 (MQ=255)


                                                               cgcgGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                          <  1:3556455/101‑8 (MQ=0)


                                                                 cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                     <  1:5591954/101‑1 (MQ=255)


                                                                    gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                  <  2:6670335/101‑1 (MQ=255)


                                                                    gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                  <  1:1585959/101‑1 (MQ=255)


                                                                    gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                  <  1:340206/101‑1 (MQ=255)


                                                                      gCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                                >  1:5332705/1‑101 (MQ=255)


                                                                       cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                               <  1:52113/101‑1 (MQ=255)


                                                                         tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                             >  2:1227194/1‑101 (MQ=255)


                                                                           gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                           <  1:2767352/101‑1 (MQ=255)


                                                                             gcGAACCC.GCCG.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGa                         <  2:2156340/100‑1 (MQ=255)


                                                                               gAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                       <  2:61725/101‑1 (MQ=255)


                                                                                  aCA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                    <  2:3363490/101‑1 (MQ=255)


                                                                                        cT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc               >  2:3561792/1‑101 (MQ=255)


                                                                                         t.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt              <  2:4905796/101‑1 (MQ=255)


                                                                                              cg.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccc          <  1:2160954/101‑1 (MQ=255)


                                                                                               gccgggcccgtCG..GCT.TCCGG.CTT..TGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcc            <  2:655325/91‑1 (MQ=37)


                                                                                               g.gC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc         >  1:2489390/1‑101 (MQ=255)


                                                                                               g.gC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc         >  1:2032115/1‑101 (MQ=255)


                                                                                               g.gC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCCTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc         >  2:3451264/1‑101 (MQ=255)


                                                                                                  c.cGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcg       <  1:3847628/101‑1 (MQ=255)


                                                                                                    cGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgc      <  1:4186446/101‑1 (MQ=255)


                                                                                                     gCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg     >  1:3972070/1‑101 (MQ=255)


GGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGG  >  NC_014034/1396851‑1397051

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG