Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,612,426 C→A 1.9% intergenic (+104/‑257) purN → / → rnd2 phosphoribosylglycinamide formyltransferase/ribonuclease D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,612,4260C→A 1.9% 2.7 ‑2.8 ‑0.0261intergenic (+104/‑257)purN/rnd2phosphoribosylglycinamide formyltransferase/ribonuclease D
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (132/124):  total (137/124)
Fisher's exact test strand distribution p-value = 6.17e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.79e-01

Only 100 of 308 total aligned reads displayed.

CGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAGGC  >  NC_014034/1612326‑1612526
                                                                                                      |                                                                                                     
 gATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCa                                                                                                      >  1:3730438/1‑101 (MQ=255)
 gATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCa                                                                                                      >  1:5194465/1‑101 (MQ=255)
 gATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCa                                                                                                      >  2:5435616/1‑101 (MQ=255)
  acccGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCAt                                                                                                     <  2:3119902/99‑1 (MQ=255)
   tCCGCTGATCTGCCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATg                                                                                                    <  1:6310210/101‑1 (MQ=255)
   tCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATg                                                                                                    <  2:4598632/101‑1 (MQ=255)
   tCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATg                                                                                                    <  2:82282/101‑1 (MQ=255)
    ccGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATgg                                                                                                   >  1:1954744/1‑101 (MQ=255)
     cGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATggc                                                                                                  >  1:4368036/1‑101 (MQ=255)
        tGATCTGACCGAGGCCATGGCCGAGGTCCCGCGCCACATTGGCCCGGTGACCGCCGGGGGCCCAGAGG..CCTCCGGCTGGGCCCGCCCCCCCCCATggcggg                                                                                               >  2:1511090/1‑101 (MQ=2)
        tGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATggcggg                                                                                               >  1:4105040/1‑101 (MQ=255)
             tGACCGAGGCCATCGCCGGGGTCACGCGACACATGGGCCCCGGGCCCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCAt                                                                                          <  1:1209334/101‑1 (MQ=17)
             ggcccGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCAt                                                                                          <  2:128986/98‑1 (MQ=255)
              gACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCAtt                                                                                         <  2:6848139/101‑1 (MQ=255)
               cccGGGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGC.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTc                                                                                        <  1:104128/100‑1 (MQ=255)
               cccGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTc                                                                                        <  2:3380462/100‑1 (MQ=255)
               acgggggCCACCGCCGAGGCCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTc                                                                                        <  2:6977366/96‑1 (MQ=255)
               aCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGGCTGCGCCCG.CCCCGCCCATGGCGGgcgccatc                                                                                        >  1:3551666/1‑97 (MQ=255)
                ccGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGGAGTcg                                                                                       >  1:246678/1‑101 (MQ=255)
                ccGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCCTTcg                                                                                       >  1:4860788/1‑101 (MQ=255)
                 cGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.GCCCGCCCATGGCGGgggccgttgc                                                                                      >  2:2143964/1‑95 (MQ=17)
                  gAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGGGCATTcgcg                                                                                     >  1:2074764/1‑101 (MQ=255)
                  gAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCGTTcgcg                                                                                     >  2:6123669/1‑101 (MQ=255)
                  gAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCGCTcgcg                                                                                     >  2:1166473/1‑101 (MQ=255)
                  gAGGCAATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGGGCATTcccc                                                                                     >  1:6858437/1‑98 (MQ=255)
                     gCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGGGCCCTCGcgccc                                                                                  >  1:6503130/1‑100 (MQ=255)
                      cgctCGCCGAGGTCACGCGACACATGGGCCCCGTGACCGCCGGGGGCCCAGAGC.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGcgccgc                                                                                 <  2:1765153/98‑1 (MQ=17)
                      ccATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGcgcccc                                                                                 >  2:2978770/1‑99 (MQ=255)
                          cGCGGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCCTTCGAGCCCCCggg                                                                             >  1:5611721/1‑101 (MQ=18)
                          cGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGGGCAATCGCGGCGCCcgg                                                                             >  1:4952986/1‑101 (MQ=18)
                          cGCCGAGGCCCCGCGACCCATTGGCCCCGTGCCCGCCGGGGGCCCGGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCggg                                                                             <  1:3422493/101‑1 (MQ=14)
                           gCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCCCCgcgg                                                                            >  2:719879/1‑101 (MQ=255)
                            ccGGGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGc                                                                           <  1:730456/101‑1 (MQ=255)
                             cGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCCATCGCGCCCCCcggggg                                                                          >  2:5273499/1‑99 (MQ=17)
                             cGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGCGGCg                                                                          >  1:5814315/1‑101 (MQ=255)
                              gAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCCCTCGCgccgcccgcgggg                                                                         >  2:6361210/1‑94 (MQ=18)
                               aGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCCCCCGGGCggg                                                                        >  1:6047079/1‑101 (MQ=255)
                               aGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCCACATCGCGCCCCCCGGGCggg                                                                        >  1:6621963/1‑101 (MQ=17)
                                   cACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCCTTCGCGCCCCCCGGGCggggggc                                                                    >  1:190889/1‑97 (MQ=17)
                                    ccgcgGCACATTGGCCCGGTGCCCGCCGGGGGCCGAGGGG.CCCTGGGCCGGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCa                                                                   <  1:2925108/100‑1 (MQ=17)
                                       cgACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGc                                                                <  1:549454/101‑1 (MQ=255)
                                       cgACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGc                                                                <  2:3205201/101‑1 (MQ=255)
                                         ccacaTTGCCCCCGTGCCCGCGGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCt                                                              <  2:1423579/100‑1 (MQ=255)
                                          cacaTTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCCTTCGCGCCGCCCGGGGGGGGCGCACGCCTc                                                             >  1:4655825/1‑101 (MQ=14)
                                          cacaTTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCACTCCGGCCGCCCGCGCGGGGCGCGgggccc                                                             >  2:5109640/1‑97 (MQ=14)
                                               tGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGGGCCGTTGCGCCGCCGGGGCGGGGGGCCGGCCAcccccc                                                        >  2:5852450/1‑98 (MQ=14)
                                                ggCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTTGCGCCGCCGGGGGGGGGCGCCGGGCTCCcgcgc                                                       >  2:5185795/1‑101 (MQ=14)
                                                    ccGTGCCCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.Ctt                                                  <  1:4550691/101‑1 (MQ=255)
                                                      gTGCCCGCCGAGGGCCCAGAGC.CCCTCGGCCGGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTgg                                                <  1:4784257/101‑1 (MQ=255)
                                                      gTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCCCCGGGGGGGGGCGCAGGCCCCCCGCGCG.CCTgg                                                >  1:3859667/1‑101 (MQ=14)
                                                      gTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCCCGCCGCCGGGGGGGGGCGCAGGCCCCCCGCGCG.CTTgg                                                >  1:2787054/1‑101 (MQ=17)
                                                       tGACCGCCGGGGGCCCAAAGG.CCCTCGGCCTGCGCCCG.CCCCGCCATCGGCGGGCGCCCTCCCGCCCCCCGGGCGGGGGGCCGGGCCCCCGCCCG.CTTggg                                               >  1:5850820/1‑101 (MQ=2)
                                                        gcccgcggGGGCCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTgggg                                              <  2:3437623/99‑1 (MQ=255)
                                                        gACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTTGCGCCGCCGGGGCGGGGCGCCGGGCTCCCGCGCG.CCTgggg                                              >  1:5322138/1‑101 (MQ=14)
                                                         aCCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCCCCGGGGCGGGGCGCCGGCCTCCCCCCCG.CCTggggg                                             >  2:6667381/1‑100 (MQ=14)
                                                            gccgAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTTGCGCCGCCGGGGCGGGGCGCAGGCCCCCCGCGCG.CCTGGGGGggg                                          >  2:4432461/1‑100 (MQ=14)
                                                            gccgAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCCCCGGGGGGGGACGCCAGCCCCCCCCGCG.CCTGGGGGggg                                          >  2:4030921/1‑100 (MQ=14)
                                                            cccgAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGc                                          <  2:3543513/100‑1 (MQ=255)
                                                             ccgAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCCGGGCGGGGCGCAGGGCTCCCGCGCC.CCTGGGGGGGCg                                         >  2:675437/1‑101 (MQ=14)
                                                              cgAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGGGCATTCGCGCCCCCCGGGGGGGGCGCCGGCCCCCCCCCCG.CCTGGGGGGGCgg                                        >  1:2572104/1‑100 (MQ=14)
                                                               gAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCCTTCGCGCCGCCGGGGGGGGACGCAGGCCTCCCGCGCG.CTTGGGGGGGCGAt                                       >  2:4227590/1‑101 (MQ=255)
                                                               gAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCCTTCGCGCCGCCGGGGCGGGGCGCAGGCCCCCCCCGCGCCTTGGGGGggggt                                        >  1:1010485/1‑98 (MQ=14)
                                                                ggggCCCCGGGGCCCCTCGGCCGGCCCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGAt                                       <  1:5266540/100‑1 (MQ=18)
                                                                aGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGGGCCTTCCCGCCCCCGGGGCGGGGCGCACGCCCCCCCGGCC.GTTGGGGGggggcgc                                      >  1:1706896/1‑96 (MQ=2)
                                                                aGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGGCGCAGGCCTCCCCCGCC.CCTGGGGGgggggtc                                      >  1:3130681/1‑96 (MQ=14)
                                                                 ggccccAGGGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCt                                     <  2:351654/98‑1 (MQ=255)
                                                                     ccggggg.CCCGCGGCCGGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTc                                 <  1:6965768/96‑1 (MQ=255)
                                                                     ccggagG.CCCCCGGCCTGCCCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTc                                 <  1:2156518/98‑1 (MQ=255)
                                                                     ccAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCCGGGGGGGGCGCAGGCCTCCCGCGCG.CTTGGGGAGGCGAGCTTGTc                                 >  2:4109819/1‑101 (MQ=18)
                                                                      cggagG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCa                                <  2:5799896/99‑1 (MQ=255)
                                                                      ccggggccccTGGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTc                                 <  2:1040622/94‑1 (MQ=255)
                                                                        gagC.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCAcc                              <  2:190886/101‑1 (MQ=255)
                                                                         agG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCCGGCCCCCCGCGCG.CTTGGGGGGGCGATCTTGTCACCg                             >  2:6239840/1‑101 (MQ=255)
                                                                             cccTCGGCCGGCCCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCt                          <  2:198769/101‑1 (MQ=255)
                                                                               cgcGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTg                        <  2:3941129/99‑1 (MQ=255)
                                                                               cTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGGGCATTTGCGCCGCCGGGGGGGGGCGCAGGGCGCCCCCGCG.CGTGGGGGGGGGATCTTGTGCCCGGCGTg                        >  2:2262649/1‑101 (MQ=9)
                                                                                tCGGCGTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTgg                       <  1:2312348/101‑1 (MQ=255)
                                                                                 cGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGt                      <  1:6402120/101‑1 (MQ=255)
                                                                                  ggCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGGCGCAGGCCTCCCGCGCG.CTTGGGGGGGCGATCTTGTCACCGGCtttggt                     >  1:4912489/1‑97 (MQ=255)
                                                                                  ggCCGGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTc                     <  1:6109936/101‑1 (MQ=255)
                                                                                   gCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCCGGCCTCCCCCGCG.CTTGGGGGGGCGATCTTGTCACCGGCTTTGTcg                    >  1:1576266/1‑101 (MQ=21)
                                                                                    ccgccccccg.ccccgcCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTcgc                   <  2:6447237/95‑1 (MQ=255)
                                                                                     cTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCtttggcccg                  >  2:6530434/1‑94 (MQ=255)
                                                                                       gcgcCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGAc                <  2:2566973/101‑1 (MQ=255)
                                                                                       gcgcCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGAc                <  2:4367814/101‑1 (MQ=255)
                                                                                          cccg.ccccgcCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGAcggg             <  1:4412019/101‑1 (MQ=255)
                                                                                            cg.ccccgcccATGGCGGGGGCCTTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CCTGGGGGGGCGGTCTTGTCCTCGGCTTGGTCCCGGcggggg           >  2:2826321/1‑99 (MQ=18)
                                                                                             g.ccccgcccATGGCGGGCGCATTCGCGCCGCCGGGGGGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGAGCTTGTCACCCGCTTGGGCGCGAccggcgg          >  1:5663824/1‑101 (MQ=25)
                                                                                             g.ccccgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGGCGCAGGCCTCCCGCGCG.CTTGGGGAGGCGGTCTTGTCACCGGCTTTGTTGCGGccggcgg          >  1:2115834/1‑101 (MQ=18)
                                                                                             g.ccccgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTTGGTGGGAcgggggg          >  2:6306742/1‑101 (MQ=21)
                                                                                             g.ccccgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGAcgggcgg          <  2:3588004/101‑1 (MQ=255)
                                                                                             g.ccccgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGAAGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTTGTcgcggccggggg          >  2:2629930/1‑93 (MQ=25)
                                                                                               ccccgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGGCGCGGccggcggg         >  2:5374088/1‑101 (MQ=255)
                                                                                                cccgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGGCGCGGCGGGCgggg        >  2:4701896/1‑101 (MQ=255)
                                                                                                 ccgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTGGCGACGGGCggggg       >  2:309901/1‑100 (MQ=255)
                                                                                                  cgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGaa      <  1:7079703/101‑1 (MQ=255)
                                                                                                    cccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAg    <  2:565172/101‑1 (MQ=255)
                                                                                                    cccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAg    <  2:5513489/101‑1 (MQ=255)
                                                                                                    cccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAg    <  1:802021/101‑1 (MQ=255)
                                                                                                    cccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAg    <  1:2424409/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
CGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGG.CCCTCGGCCTGCGCCCG.CCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAGGC  >  NC_014034/1612326‑1612526

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG