BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,734,908	T→G	1.4%	intergenic (‑161/+32)	RCAP_rcc01603 ← / ← RCAP_rcc01604	hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,734,908	0	T→G	1.4%	2.5 ‑2.8 ‑0.0	360	intergenic (‑161/+32)	RCAP_rcc01603/RCAP_rcc01604	hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (5/0): ref base (180/175): total (185/175)
Fisher's exact test strand distribution p-value = 6.12e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.92e-01

Only 100 of 377 total aligned reads displayed.


GCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTT  >  NC_014034/1734809‑1735008


gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCAt                                                                                                      >  2:946394/1‑101 (MQ=255)


 cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATa                                                                                                     <  2:1045509/101‑1 (MQ=255)


  gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATaa                                                                                                    >  1:5396155/1‑101 (MQ=255)


   tcggcagACGGTCCGGGCAGGGGTGCGCTGCCCC.CCGTCCTGCGCACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATaaa                                                                                                   <  2:3035703/95‑1 (MQ=14)


   tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATaaa                                                                                                   <  1:7017028/101‑1 (MQ=255)


   tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATaaa                                                                                                   <  2:1761788/101‑1 (MQ=255)


   tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATaaa                                                                                                   >  2:6555055/1‑101 (MQ=255)


   tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATaaa                                                                                                   <  1:4350512/101‑1 (MQ=255)


    cAGCCTACGGGCCTGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAat                                                                                                  <  2:2282432/101‑1 (MQ=17)


    cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAat                                                                                                  <  2:6221839/101‑1 (MQ=255)


    cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAat                                                                                                  >  1:218161/1‑101 (MQ=255)


     aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAata                                                                                                 <  2:6494078/101‑1 (MQ=255)


      gCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAatat                                                                                                <  2:2618494/101‑1 (MQ=255)


       ccGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATc                                                                                               <  1:7092982/101‑1 (MQ=255)


       ccGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATc                                                                                               <  1:1113648/101‑1 (MQ=255)


       ccGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCAGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATc                                                                                               <  1:5903206/101‑1 (MQ=255)


        cGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATcc                                                                                              <  1:4711680/101‑1 (MQ=255)


           cttccgatctaGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCgg                                                                                           <  2:4110157/91‑1 (MQ=14)


            gggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCggg                                                                                          <  2:1518542/101‑1 (MQ=255)


            gggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCggg                                                                                          <  1:6117497/101‑1 (MQ=255)


              gccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGt                                                                                        >  1:5807041/1‑101 (MQ=255)


               ccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTg                                                                                       >  1:3651629/1‑101 (MQ=255)


               ccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTg                                                                                       <  1:3793242/101‑1 (MQ=255)


                ggggcTGGGGGGCGCTC.CCCGCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATGTCCCGGGGTGc                                                                                      <  2:361738/100‑1 (MQ=17)


                cgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGNCTTCATCTTGCCATAAATATCCCGGGGTGc                                                                                      >  1:3338723/1‑101 (MQ=255)


                cgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGc                                                                                      <  2:3167905/101‑1 (MQ=255)


                 gggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCg                                                                                     <  1:5172568/101‑1 (MQ=255)


                  ggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCgg                                                                                    <  2:1093664/101‑1 (MQ=255)


                     aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGTCATAAATATCACGGGGTGCGGGGc                                                                                 >  2:2023470/1‑101 (MQ=17)


                     aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGc                                                                                 <  2:5800419/101‑1 (MQ=255)


                      ggggggCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCa                                                                                <  2:1637115/101‑1 (MQ=255)


                      ggggggCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCa                                                                                >  2:2357502/1‑101 (MQ=255)


                        ggggCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCaga                                                                              <  1:449014/101‑1 (MQ=255)


                                gcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCt                                                                      >  1:3123532/3‑101 (MQ=255)


                                  ccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCa                                                                    <  2:884048/101‑1 (MQ=255)


                                  ccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGCGCAGAGCCCCGCTCa                                                                    <  1:5623954/101‑1 (MQ=255)


                                   cc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCaa                                                                   >  2:5495475/1‑101 (MQ=255)


                                      ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAAcc                                                                 <  1:4312509/101‑1 (MQ=255)


                                        gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                               >  2:714239/1‑101 (MQ=255)


                                        gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                               >  1:4651167/1‑101 (MQ=255)


                                        gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                               >  1:1797223/1‑101 (MQ=255)


                                        gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                               >  2:35392/1‑101 (MQ=255)


                                         tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGaa                                                              <  1:5870510/101‑1 (MQ=255)


                                          ccTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGGGCGGGGCAGAGCCCCGCTCAACCGAAg                                                             >  2:5908243/1‑101 (MQ=255)


                                           cTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGNGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                                            >  1:2631159/1‑101 (MQ=255)


                                              cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                                         >  1:4836940/1‑101 (MQ=255)


                                              cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                                         >  1:1036477/1‑101 (MQ=255)


                                                gACTCCCCCCGGGATATTTATGGCAAGATGAATCTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                                       <  2:329526/101‑1 (MQ=255)


                                                  cTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                                     <  2:5240431/101‑1 (MQ=255)


                                                   tCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGa                                                    >  1:6699167/1‑101 (MQ=255)


                                                      ccccGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                                                 <  1:753740/101‑1 (MQ=255)


                                                      ccccGGGATATTTAAGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                                                 <  1:5285122/101‑1 (MQ=255)


                                                         cGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                                              >  2:4960481/1‑101 (MQ=255)


                                                          gggATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                                             >  2:380304/1‑101 (MQ=255)


                                                            gATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                                           >  2:6794514/1‑101 (MQ=255)


                                                            gATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                                           >  2:6364948/1‑101 (MQ=255)


                                                            gATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                                           >  1:443999/1‑101 (MQ=255)


                                                             atatTTATGGCAGGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGGTCCGCGGccc                                          >  1:5076591/1‑101 (MQ=255)


                                                                tttATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                       >  1:1561976/1‑101 (MQ=255)


                                                                  tATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                                     <  1:1328464/101‑1 (MQ=255)


                                                                  tATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                                     >  2:5109002/1‑101 (MQ=255)


                                                                  tATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                                     >  1:2828973/1‑101 (MQ=255)


                                                                   aTGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                                    >  2:4348950/1‑101 (MQ=255)


                                                                      gCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                                 >  1:3490581/1‑101 (MQ=255)


                                                                       cAAGATGAAACTGCAGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCt                                <  1:2989964/101‑1 (MQ=255)


                                                                        aaGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                               >  2:6879180/1‑101 (MQ=255)


                                                                        aaGATGAAACTGCGGCCTTCATCTAGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                               >  2:3402552/1‑101 (MQ=255)


                                                                         aGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtga                              <  1:4191169/101‑1 (MQ=255)


                                                                           aTGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg                            >  1:2299060/1‑101 (MQ=255)


                                                                            tGAAACTGCGGCCTTCATCTTGCCATAAATATCCCTGGGGGCGGGTCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCTGGTTTTCGCtgatga                           >  2:7071566/1‑101 (MQ=255)


                                                                            tGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga                           <  2:125799/101‑1 (MQ=255)


                                                                             gAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg                          <  2:4721566/101‑1 (MQ=255)


                                                                               aaCTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                        <  1:882696/101‑1 (MQ=255)


                                                                               aaCTGCGGCCTTCATCTTGCCATAAATATCCCGGGGGGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                        <  1:1745171/101‑1 (MQ=255)


                                                                                aCTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                       <  1:6813387/101‑1 (MQ=255)


                                                                                aCTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                       <  1:1429450/101‑1 (MQ=255)


                                                                                aCTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                       >  1:1927639/1‑101 (MQ=255)


                                                                                aCTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                       <  2:4824557/101‑1 (MQ=255)


                                                                                  tGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc                     <  1:4148268/101‑1 (MQ=255)


                                                                                  tGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc                     >  1:385786/1‑101 (MQ=255)


                                                                                   gCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgcg                    <  2:3328628/101‑1 (MQ=255)


                                                                                      gCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGccc                 <  1:649583/101‑1 (MQ=255)


                                                                                       ccTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCg                >  1:6369806/1‑101 (MQ=255)


                                                                                       ccTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCg                >  2:6623013/1‑101 (MQ=255)


                                                                                       ccTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCg                <  1:1290354/101‑1 (MQ=255)


                                                                                        cTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGt               >  2:5048702/1‑101 (MQ=255)


                                                                                        cTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGt               >  2:5411512/1‑101 (MQ=255)


                                                                                         ttCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt              <  1:6956123/101‑1 (MQ=255)


                                                                                          tCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc             <  2:4074190/101‑1 (MQ=255)


                                                                                          tCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc             <  1:1651363/101‑1 (MQ=255)


                                                                                           cATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCg            <  2:59838/101‑1 (MQ=255)


                                                                                               ttGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAg        >  2:273947/1‑101 (MQ=255)


                                                                                                tGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg       >  2:585085/1‑101 (MQ=255)


                                                                                                tGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg       >  1:2053515/1‑101 (MQ=255)


                                                                                                 gCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGt      <  1:3967199/101‑1 (MQ=255)


                                                                                                   cATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCg    >  2:2222549/1‑101 (MQ=255)


                                                                                                   cATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCg    <  2:6995907/101‑1 (MQ=255)


                                                                                                     tAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGtt  >  1:3549057/1‑101 (MQ=255)


                                                                                                     tAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGtt  <  1:1005401/101‑1 (MQ=255)


                                                                                                     tAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCCCCCGTTCGGCAGTACGtt  >  2:281059/1‑101 (MQ=255)


GCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGCGGCCTTCATCTTGCCATAAATATCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTT  >  NC_014034/1734809‑1735008

Base quality scores:


ATCG

< 3 ≤


ATCG

< 23 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG