Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,069 G→C 24.9% T196T (ACC→ACG RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0690G→C24.9% 0.0 ‑0.6 ‑0.0899T196T (ACC→ACGRCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (110/115):  ref base (347/327):  total (457/442)
Fisher's exact test strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.14e-01

Only 100 of 1196 total aligned reads displayed.

CCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACA  >  NC_014034/221969‑222169
                                                                                                    |                                                                                                    
 cgtgggatcggtgggggccgtggggtccgtcggATCCGTGGGCTGGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGTGGGAtcgg                                                                                                     <  1:6389004/101‑1 (MQ=11)
 cgtgggatcggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGAGGGATCGGGCGGCGCCGTGGGATCGGGCGGGTCCCTGGGAtcgg                                                                                                     >  2:2550454/1‑101 (MQ=11)
 cggggggtcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcgg                                                                                                     <  1:1101313/98‑1 (MQ=11)
  gtgggatcggtcgggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGGCCGTGGGGTCGGTCGGGTCCGAGGGATCGAGCGGCACCGTGGGAtcggc                                                                                                    >  2:265031/1‑100 (MQ=11)
   tggtgtcggtcgggtccgtgggctcggtcggCTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCTGTGGGAtcggtc                                                                                                   <  1:5121494/96‑1 (MQ=14)
   tggggtccgtcggatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGCGGGGtcggtc                                                                                                   >  2:218734/1‑101 (MQ=11)
   tggcgtcggtcggatccgtggggtcggtcggGTCCGAGGGATCGGTCGGGGCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                                                                                                   <  1:257477/96‑1 (MQ=11)
    gggatcggtcgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGAtctgtcg                                                                                                  >  1:5311154/1‑101 (MQ=11)
      gatcggtcggatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGGGGGATCGGTGGGGTCGGTGTGATCGgtcggg                                                                                                >  2:5012217/1‑101 (MQ=14)
        tcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcggatc                                                                                              >  2:5491416/1‑101 (MQ=11)
        tcggtcgggtccgtgggatcggtcggGTCCCTCGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtc                                                                                              <  2:1327369/101‑1 (MQ=11)
         cgggctgggccgtcggatctgtcggATCCGTAGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtcc                                                                                             <  1:2686788/92‑1 (MQ=11)
         ccggcggagccgtgggatcgggcagATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcggatcc                                                                                             <  1:5408185/92‑1 (MQ=11)
          ggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcggatccg                                                                                            >  1:5643725/1‑101 (MQ=11)
          ggtcgggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGTGGGATCGGTCGGGGCGGTGGGCTCGgtggggtccg                                                                                            >  2:5645210/1‑101 (MQ=11)
          ggtcggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccg                                                                                            >  1:4480217/1‑101 (MQ=11)
           gtcggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgt                                                                                           <  2:2423601/101‑1 (MQ=11)
            tgttatccgggggatcgtgtgtATCCGTAGGATCGGTCGTGGCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGgtcgggtccgtc                                                                                          <  2:6701496/96‑1 (MQ=2)
             cgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtcg                                                                                         >  1:4933803/1‑101 (MQ=11)
             cgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgatg                                                                                         >  2:7094162/1‑98 (MQ=11)
             cggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcg                                                                                         >  2:5347959/1‑101 (MQ=11)
              ggttccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggg                                                                                        <  2:4000300/98‑1 (MQ=11)
              gggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGGCCGGGGGGGCCGTCGGATCCGGGGGATAGGTCGGGTCCGGGGGACCGggcgggaccggggg                                                                                        >  1:3947655/1‑97 (MQ=2)
              gggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGTGGGATCGGGCGGGGCCGTGGGATGGgtcgggtccttggg                                                                                        >  1:84199/1‑96 (MQ=11)
              ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATGCGTGGGATCGGACGGGTTCGTGGGATCGgtcgggtccgtggg                                                                                        >  2:892953/4‑101 (MQ=14)
                gtccgtgggatcggtcggATCCGTGGGATCGGTCTGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGCCGGATCCGGGCGATCGgtccgttccgcgagag                                                                                      >  2:2059915/1‑95 (MQ=11)
                ggccgtgggcgggggcggAGCGGTGGGGTCGGTCGGGTGCGTGGGATCGGTCGGCTCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggat                                                                                      <  1:3308605/99‑3 (MQ=14)
                  ccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggggtcg                                                                                    >  1:4119702/1‑100 (MQ=11)
                  ccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcgggtcc                                                                                    <  1:5887939/101‑1 (MQ=11)
                   cgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGtggggtccgtcgggtccg                                                                                   <  2:6973366/101‑1 (MQ=14)
                     ggggatctgtcggGGCCGTGGCTTCCGCCGGGTCCGTGGGACCGGCCGGACCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTcggatccgtgggatcggtc                                                                                 <  2:4157346/100‑2 (MQ=2)
                     cgtccttcgtctgATCCGTGTGATCTGTCGGCTCCGTGCGCTCGCTCGGCTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgtc                                                                                 <  1:2271641/93‑2 (MQ=2)
                      ggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgtcg                                                                                >  1:5578839/1‑99 (MQ=11)
                      gggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTgg                                                                                >  1:5740131/1‑101 (MQ=11)
                      aggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTgg                                                                                <  1:178635/100‑1 (MQ=14)
                       ggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTggg                                                                               >  1:2818372/1‑101 (MQ=14)
                       ggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTggg                                                                               >  1:2017793/1‑101 (MQ=14)
                        ggtcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTgggg                                                                              <  2:2851123/99‑2 (MQ=11)
                         gtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCAGATCCGTCGGGTCCGTGGGac                                                                             >  2:5814807/8‑100 (MQ=14)
                         atcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTggggt                                                                             >  2:6266210/1‑99 (MQ=11)
                          tccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtc                                                                            <  2:2721276/98‑1 (MQ=11)
                           cggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCGGTCGGGTCGGTgggggcg                                                                           >  2:2929820/1‑97 (MQ=14)
                           cggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtcc                                                                           <  2:4585312/101‑2 (MQ=11)
                           ccgtgggATCCGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcg                                                                           <  2:4711708/96‑1 (MQ=17)
                             gtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCATCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtccgt                                                                         <  1:4235703/101‑1 (MQ=11)
                             gtccgATCCGTTGTTTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGAtccgt                                                                         <  2:1613359/101‑1 (MQ=11)
                              tcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtc                                                                        >  1:1195202/1‑101 (MQ=11)
                               gggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcggtcg                                                                       >  1:3054100/2‑101 (MQ=17)
                               cggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTGGGGTCCGTGGGGTCGGTCGGATCGGTGGGGGCAGTGCGAtcggtcg                                                                       >  2:3005723/1‑101 (MQ=11)
                               cggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAgcggtcg                                                                       >  1:2399213/1‑101 (MQ=14)
                               cggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtccgtgg                                                                       <  2:6177014/101‑3 (MQ=11)
                                gggtCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcgg                                                                      <  1:6542474/98‑1 (MQ=14)
                                ggATCGGTCGGATCCGTGGGGTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcggtcgg                                                                      <  2:1779679/101‑1 (MQ=17)
                                 gatcgggcggATCCGTGGGGTCCGTTGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGa                                                                     <  2:4327388/93‑1 (MQ=17)
                                 gATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGGGGGATCGGGCGGGGCCGGGGGGTGCGTCGGacacgggggg                                                                     >  2:3310248/1‑92 (MQ=11)
                                  gtCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtcgggt                                                                    <  2:6398612/100‑3 (MQ=11)
                                  gtCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGGCCGTGGGAtcggtcgggt                                                                    >  2:5110082/2‑99 (MQ=11)
                                  gtCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcgggt                                                                    <  1:957758/100‑3 (MQ=14)
                                  aTCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGAt                                                                    >  1:386015/1‑101 (MQ=17)
                                   tCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATc                                                                   <  1:1714764/101‑1 (MQ=11)
                                   tCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTc                                                                   <  2:3473283/101‑1 (MQ=14)
                                      gccgagtcggtgggGTCCGTGGGATCGGTCGGGACCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGt                                                                <  2:6062759/95‑1 (MQ=18)
                                       tcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTg                                                               >  1:4778679/3‑101 (MQ=17)
                                        aggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGGTCGGTCGGGTCCGtcg                                                              >  2:1900233/2‑99 (MQ=14)
                                         gggtgggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTggg                                                             <  1:1566030/96‑1 (MQ=14)
                                         ggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGGCCGTGGGGTCGGTCGGGTcgggcgg                                                             >  2:3780849/1‑95 (MQ=14)
                                          gATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGa                                                            >  1:6796199/1‑101 (MQ=17)
                                          gATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGa                                                            <  2:485667/101‑1 (MQ=255)
                                            tcggtcggGGCGCTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtc                                                          <  2:800095/101‑1 (MQ=14)
                                             cggtcggGTCCGGGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcg                                                         <  1:3619652/101‑1 (MQ=17)
                                             cctgggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcg                                                         <  2:44608/96‑1 (MQ=255)
                                               gtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggt                                                       >  1:6687179/1‑101 (MQ=17)
                                               gtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTGCGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggt                                                       <  2:6861234/101‑1 (MQ=14)
                                               ggggggTCCGGGGGATCGGGCGGGTCCGGGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggt                                                       <  1:711214/98‑1 (MQ=18)
                                                tcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggtc                                                      <  1:461279/101‑1 (MQ=17)
                                                    ggccGTGGGCTCGGTCGGGTCCGTGGGCTCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGt                                                  <  2:3128811/99‑1 (MQ=255)
                                                      ccGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGGCCGGGGGATCGGCCGGGTcc                                                >  2:5228954/1‑101 (MQ=17)
                                                      ccGTCGGGTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTcc                                                <  2:3624617/101‑1 (MQ=17)
                                                        gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGt                                              >  1:2916117/1‑101 (MQ=1)
                                                          gggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGtag                                            >  1:2126563/1‑99 (MQ=17)
                                                          cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGCTCGGTCGGGTCCGTCGGATCGGTCGGGTCCGGgg                                            >  1:3489249/2‑101 (MQ=17)
                                                          cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgg                                            >  1:3169683/2‑101 (MQ=17)
                                                           ggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTggg                                           >  2:5939173/1‑101 (MQ=17)
                                                            gATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggg                                          >  1:1898061/1‑101 (MQ=255)
                                                             aTCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGGCCGGGGGGc                                         >  1:1022575/1‑101 (MQ=255)
                                                               cggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcg                                       >  1:6177333/1‑97 (MQ=14)
                                                                ggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTaggatcag                                      >  2:3234716/1‑96 (MQ=14)
                                                                ggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcgg                                      <  1:2487480/101‑6 (MQ=14)
                                                                 gtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggg                                     >  2:3693105/1‑95 (MQ=14)
                                                                  tcggactggggggATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggtg                                    <  1:6063849/91‑1 (MQ=18)
                                                                   cggGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGCCCGTACGATCGGTCGGCTCCGTcgggtcagccg                                   >  2:6546028/1‑94 (MQ=2)
                                                                   cggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcg                                   >  2:1488205/1‑93 (MQ=14)
                                                                      gtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAt                                <  2:1086612/100‑1 (MQ=255)
                                                                           tGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtc                           >  1:3401494/1‑101 (MQ=255)
                                                                                tcgttggtgTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGatc                      <  2:1183788/93‑3 (MQ=11)
                                                                                  ggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGc                    >  1:1359499/1‑101 (MQ=255)
                                                                                   gtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGCGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCt                   <  1:5019965/101‑1 (MQ=255)
                                                                                      gggTCCGTGGGATCGGTCCGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                <  2:3910001/101‑1 (MQ=255)
                                                                                        gTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGGCGGGGCCGTGGGGCTGGTGGGGTCGGTCGGACCGCGGTcgc              >  2:2296364/1‑101 (MQ=25)
                                                                                            gTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgcc          >  1:7013978/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACA  >  NC_014034/221969‑222169

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG