Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,333 G→C 1.9% intergenic (‑129/‑176) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3330G→C 1.9% 6.4 ‑0.3 ‑0.1361intergenic (‑129/‑176)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (3/4):  ref base (181/172):  total (184/177)
Fisher's exact test strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.25e-01

Only 100 of 393 total aligned reads displayed.

TGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCG  >  NC_014034/2249234‑2249432
                                                                                                       |                                                                                                        
tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTATGCCCCC.GTCCCGTGCCGGGACTCCCTCGGGATATTTGAGGCAAGATGAAgggg..gg                                                                                                          <  2:4561484/101‑1 (MQ=39)
 gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGc                                                                                                         >  1:4547336/1‑101 (MQ=255)
  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCt                                                                                                        <  2:6931060/101‑1 (MQ=255)
  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCt                                                                                                        >  1:5213294/1‑101 (MQ=255)
  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCt                                                                                                        >  1:2970890/1‑101 (MQ=255)
  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCt                                                                                                        <  1:2723189/101‑1 (MQ=255)
  cacaGATACCGCAGCTGGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCt                                                                                                        <  2:6541101/101‑1 (MQ=255)
   acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCtt                                                                                                       <  2:50145/101‑1 (MQ=255)
    caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCttt                                                                                                      >  2:2983964/1‑101 (MQ=255)
     aGATGCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTc                                                                                                     <  1:5713385/101‑1 (MQ=255)
     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTc                                                                                                     <  1:4127248/101‑1 (MQ=255)
     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTc                                                                                                     <  2:3634720/101‑1 (MQ=255)
     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTc                                                                                                     <  1:5933804/101‑1 (MQ=255)
       aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCAt                                                                                                   >  2:1482032/1‑101 (MQ=255)
              aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCg                                                                                            >  2:1906171/1‑101 (MQ=255)
               gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGa                                                                                           >  1:6156798/1‑101 (MQ=255)
                cTTGGTCACCCGGGGAAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGaa                                                                                          >  1:6863780/1‑101 (MQ=38)
                cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGaa                                                                                          >  1:4227783/1‑101 (MQ=255)
                  tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTAATCTTGCCGagaa                                                                                        >  2:3300747/1‑101 (MQ=38)
                   gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAat                                                                                       >  2:5280081/1‑101 (MQ=255)
                    ggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAata                                                                                      <  1:6618084/101‑1 (MQ=255)
                      cACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                    >  1:382347/1‑101 (MQ=255)
                        cccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                                  >  2:859900/1‑101 (MQ=255)
                         ccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                 <  1:6379156/101‑1 (MQ=255)
                          cGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                <  2:561713/101‑1 (MQ=255)
                           ggggCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                                               >  2:2198174/1‑101 (MQ=255)
                              gCAAGAAGGGGGCTCTG.CCCCTGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                            <  2:1181186/101‑1 (MQ=255)
                                 agaagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                         <  1:366746/101‑1 (MQ=255)
                                   aagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                      >  2:2718343/1‑101 (MQ=255)
                                   aagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                      >  2:4112686/1‑101 (MQ=255)
                                   aagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                      <  2:6189599/101‑1 (MQ=255)
                                   aagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                      >  1:5555789/1‑101 (MQ=255)
                                    agGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                     >  2:166822/1‑101 (MQ=255)
                                    agGGGGCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                     <  2:2666785/101‑1 (MQ=255)
                                     gggggCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tgg                                                                    <  1:1188798/101‑1 (MQ=255)
                                       gggCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                                  <  1:1009599/101‑1 (MQ=255)
                                       gggCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                                  >  1:2913370/1‑101 (MQ=255)
                                        ggCTCTGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                 >  2:6075470/1‑101 (MQ=255)
                                          ctctGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                             >  2:440226/1‑101 (MQ=255)
                                          ctctGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                             >  2:1947672/1‑101 (MQ=255)
                                          ctctGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                             <  1:3419422/101‑1 (MQ=255)
                                          ctctGCCCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                             <  2:1547515/101‑1 (MQ=255)
                                           tctgccccc.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                                            >  1:1436022/6‑101 (MQ=255)
                                             tgccccc.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                                          <  1:5088714/98‑1 (MQ=255)
                                              gccccc.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                         >  2:5410155/3‑101 (MQ=255)
                                              gccccc.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                         >  2:2925381/3‑101 (MQ=255)
                                                 ccc.gtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                      >  2:3838024/1‑101 (MQ=255)
                                                 ccc.gtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                      >  1:5460727/1‑101 (MQ=255)
                                                  cc.gtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                     <  1:1987405/101‑1 (MQ=255)
                                                      tcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                  <  2:1059368/101‑1 (MQ=255)
                                                      tcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                  >  1:6248853/1‑101 (MQ=255)
                                                       cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                 <  1:3041851/101‑1 (MQ=255)
                                                       cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                 <  1:3350564/101‑1 (MQ=255)
                                                        ccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                >  1:1727764/1‑101 (MQ=255)
                                                         cgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                               <  2:3073668/101‑1 (MQ=255)
                                                         cgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                               <  2:6706884/101‑1 (MQ=255)
                                                         cgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                               >  2:1339030/1‑101 (MQ=255)
                                                          gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                              >  2:6251473/1‑101 (MQ=255)
                                                          gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                              <  1:4434498/101‑1 (MQ=255)
                                                          gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                              >  2:1023740/1‑101 (MQ=255)
                                                              cGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                                         >  1:2353522/1‑101 (MQ=255)
                                                               gggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                                        <  2:5556588/101‑1 (MQ=255)
                                                                ggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccc                                       >  2:1249176/1‑101 (MQ=255)
                                                                  aCTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTTATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccct                                     <  1:6672530/101‑3 (MQ=255)
                                                                  aCTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccct                                     <  1:5280347/101‑3 (MQ=255)
                                                                  aCTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccct                                     >  1:3128543/1‑99 (MQ=255)
                                                                  aCTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccct                                     <  2:68211/101‑3 (MQ=255)
                                                                   cTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                                    <  2:6863426/101‑4 (MQ=255)
                                                                   cTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                                    <  1:741631/101‑4 (MQ=255)
                                                                    tCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                                   <  1:6739474/101‑5 (MQ=255)
                                                                    tCCCCCGGGATATCTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                                   <  2:4337424/101‑5 (MQ=255)
                                                                    ccccccGGGATATGTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.ccccc                                       <  2:4787575/100‑1 (MQ=2)
                                                                     cccccGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttcg                                  >  2:3151444/1‑96 (MQ=255)
                                                                        ccGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgg                               <  1:208961/101‑1 (MQ=255)
                                                                        ccGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgg                               >  2:5860343/1‑101 (MQ=255)
                                                                         cGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCC.tgccc                                 >  1:1102536/1‑97 (MQ=0)
                                                                          gggATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggc                             <  1:2171587/101‑1 (MQ=255)
                                                                          gggATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggc                             >  2:1144534/1‑101 (MQ=255)
                                                                          gggATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggc                             <  1:355356/101‑1 (MQ=255)
                                                                          gggATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TAGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggc                             >  2:2394347/1‑101 (MQ=255)
                                                                            gATATTTGAGCCAAGATGAAGGGG..GGCTTTCAGCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggcgg                           <  2:717367/101‑1 (MQ=25)
                                                                               atTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcg                        >  1:481809/1‑101 (MQ=255)
                                                                                tttGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgc                       <  2:3958137/101‑1 (MQ=255)
                                                                                tttGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgc                       <  2:4182580/101‑1 (MQ=255)
                                                                                    aGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGAcg                   <  2:547596/101‑1 (MQ=255)
                                                                                    aGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGAcg                   >  2:5770225/1‑101 (MQ=255)
                                                                                         aGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACgcgcgc              <  1:4762198/101‑1 (MQ=255)
                                                                                          gATGGAGGGG..GGCTTTCATCTTGCCGAAAATTTGCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgcc             <  1:1593521/101‑1 (MQ=37)
                                                                                          gATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgcc             >  1:1339850/1‑101 (MQ=255)
                                                                                           aTGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgccg            >  2:776742/1‑101 (MQ=255)
                                                                                           aTGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGAGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgccg            >  1:7052704/1‑101 (MQ=255)
                                                                                            tGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgccgc           <  1:2206746/101‑1 (MQ=255)
                                                                                             gAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCa          <  2:2063484/101‑1 (MQ=255)
                                                                                               aGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAAc        >  2:2828444/1‑101 (MQ=255)
                                                                                                gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACa       <  1:3782101/101‑1 (MQ=255)
                                                                                                gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACa       >  1:4009044/1‑101 (MQ=255)
                                                                                                gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACa       <  1:6048378/101‑1 (MQ=255)
                                                                                                gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACa       <  2:3507362/101‑1 (MQ=255)
                                                                                                  gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCATTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg     <  1:5007893/101‑1 (MQ=255)
                                                                                                      ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc   >  2:5093846/1‑101 (MQ=255)
                                                                                                       |                                                                                                        
TGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCG  >  NC_014034/2249234‑2249432

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG